International Journal of Cell Biology最新文献_第6页

Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining 微波辅助组织准备快速固定，脱钙，抗原检索，冷冻切片和免疫染色

International Journal of Cell Biology Pub Date : 2016-10-20 DOI: 10.1155/2016/7076910

K. Katoh

引用次数: 18

Carbohydrate Moieties and Cytoenzymatic Characterization of Hemocytes in Whiteleg Shrimp Litopenaeus vannamei 凡纳滨对虾(Litopenaeus vannamei)血细胞碳水化合物组成及细胞酶特性研究

International Journal of Cell Biology Pub Date : 2016-10-19 DOI: 10.1155/2016/9032181

Norma Estrada, E. Velázquez, C. Rodríguez‐Jaramillo, F. Ascencio

引用次数: 9

Connexin's Connection in Breast Cancer Growth and Progression 连接蛋白在乳腺癌生长和进展中的作用

International Journal of Cell Biology Pub Date : 2016-08-23 DOI: 10.1155/2016/9025905

Debarshi Banerjee

{"title":"Connexin's Connection in Breast Cancer Growth and Progression","authors":"Debarshi Banerjee","doi":"10.1155/2016/9025905","DOIUrl":"https://doi.org/10.1155/2016/9025905","url":null,"abstract":"Gap junctions are cell-to-cell junctions that are located in the basolateral surface of two adjoining cells. A gap junction channel is composed of a family of proteins called connexins. Gap junction channels maintain intercellular communication between two cells through the exchange of ions, small metabolites, and electrical signals. Gap junction channels or connexins are widespread in terms of their expression and function in maintaining the development, differentiation, and homeostasis of vertebrate tissues. Gap junction connexins play a major role in maintaining intercellular communication among different cell types of normal mammary gland for proper development and homeostasis. Connexins have also been implicated in the pathogenesis of breast cancer. Differential expression pattern of connexins and their gap junction dependent or independent functions provide pivotal cross talk of breast tumor cells with the surrounding stromal cell in the microenvironment. Substantial research from the last 20 years has accumulated ample evidences that allow us a better understanding of the roles that connexins play in the tumorigenesis of primary breast tumor and its metastatic progression. This review will summarize the knowledge about the connexins and gap junction activities in breast cancer highlighting the differential expression and functional dynamics of connexins in the pathogenesis of the disease.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9025905","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64593989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Stem Cells Applications in Regenerative Medicine and Disease Therapeutics. 干细胞在再生医学和疾病治疗中的应用。

International Journal of Cell Biology Pub Date : 2016-01-01 Epub Date: 2016-07-19 DOI: 10.1155/2016/6940283

Ranjeet Singh Mahla

引用次数: 0

Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay Wnt3a对HEK293T细胞增殖的影响取决于应用的实验

International Journal of Cell Biology Pub Date : 2015-12-22 DOI: 10.1155/2015/928502

Patricia Reischmann, Johanna Fiebeck, Nadine von der Weiden, O. Müller

{"title":"Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay","authors":"Patricia Reischmann, Johanna Fiebeck, Nadine von der Weiden, O. Müller","doi":"10.1155/2015/928502","DOIUrl":"https://doi.org/10.1155/2015/928502","url":null,"abstract":"The Wnt signaling pathway has been associated with many essential cell processes. This study aims to examine the effects of Wnt signaling on proliferation of cultured HEK293T cells. Cells were incubated with Wnt3a, and the activation of the Wnt pathway was followed by analysis of the level of the β-catenin protein and of the expression levels of the target genes MYC and CCND1. The level of β-catenin protein increased up to fourfold. While the mRNA levels of c-Myc and cyclin D1 increased slightly, the protein levels increased up to a factor of 1.5. Remarkably, MTT and BrdU assays showed different results when measuring the proliferation rate of Wnt3a stimulated HEK293T cells. In the BrdU assays an increase of the proliferation rate could be detected, which correlated to the applied Wnt3a concentration. Oppositely, this correlation could not be shown in the MTT assays. The MTT results, which are based on the mitochondrial activity, were confirmed by analysis of the succinate dehydrogenase complex by immunofluorescence and by western blotting. Taken together, our study shows that Wnt3a activates proliferation of HEK293 cells. These effects can be detected by measuring DNA synthesis rather than by measuring changes of mitochondrial activity.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2015 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/928502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64167809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Integration of Mitochondrial Targeting for Molecular Cancer Therapeutics 线粒体靶向在分子癌症治疗中的整合

International Journal of Cell Biology Pub Date : 2015-12-02 DOI: 10.1155/2015/283145

P. Marchetti, P. Guerreschi, L. Mortier, J. Kluza

引用次数: 26

Effects of Activating Mutations on EGFR Cellular Protein Turnover and Amino Acid Recycling Determined Using SILAC Mass Spectrometry 激活突变对EGFR细胞蛋白质周转和氨基酸再循环的影响

International Journal of Cell Biology Pub Date : 2015-11-24 DOI: 10.1155/2015/798936

M. Greig, Sherry L. Niessen, S. Weinrich, J. Feng, Manli Shi, Ted Johnson

{"title":"Effects of Activating Mutations on EGFR Cellular Protein Turnover and Amino Acid Recycling Determined Using SILAC Mass Spectrometry","authors":"M. Greig, Sherry L. Niessen, S. Weinrich, J. Feng, Manli Shi, Ted Johnson","doi":"10.1155/2015/798936","DOIUrl":"https://doi.org/10.1155/2015/798936","url":null,"abstract":"Rapid mutations of proteins that are targeted in cancer therapy often lead to drug resistance. Often, the mutation directly affects a drug's binding site, effectively blocking binding of the drug, but these mutations can have other effects such as changing the protein turnover half-life. Utilizing SILAC MS, we measured the cellular turnover rates of an important non-small cell lung cancer target, epidermal growth factor receptor (EGFR). Wild-type (WT) EGFR, EGFR with a single activating mutant (Del 746–750 or L858R), and the drug-resistant double mutant (L858R/T790M) EGFR were analyzed. In non-small cell lung cancer cell lines, EGFR turnover rates ranged from 28 hours in A431 cells (WT) to 7.5 hours in the PC-9 cells (Del 746–750 mutant). The measurement of EGFR turnover rate in PC-9 cells dosed with irreversible inhibitors has additional complexity due to inhibitor effects on cell viability and results were reported as a range. Finally, essential amino acid recycling (K and R) was measured in different cell lines. The recycling was different in each cell line, but the overall inclusion of the effect of amino acid recycling on calculating EGFR turnover rates resulted in a 10–20% reduction in rates.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2015 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/798936","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65154700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips 一种不使用细胞离心机和细胞夹制备细胞载玻片和染色的简单有效方法

International Journal of Cell Biology Pub Date : 2015-11-17 DOI: 10.1155/2015/813216

Xiaotang Hu, V. Laguerre, Daniel Packert, Alice Nakasone, L. Moscinski

{"title":"A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips","authors":"Xiaotang Hu, V. Laguerre, Daniel Packert, Alice Nakasone, L. Moscinski","doi":"10.1155/2015/813216","DOIUrl":"https://doi.org/10.1155/2015/813216","url":null,"abstract":"Cell staining is a necessary and useful technique for visualizing cell morphology and structure under a microscope. This technique has been used in many areas such as cytology, hematology, oncology, histology, virology, serology, microbiology, cell biology, and immunochemistry. One of the key pieces of equipment for preparing a slide for cell staining is cytology centrifuge (cytocentrifuge) such as cytospin. However, many small labs do not have this expensive equipment and its accessory, cytoclips (also expensive relatively), which makes them difficult to study cell cytology. Here we present an alternative method for preparing a slide and cell staining in the absence of a cytocentrifuge (and cytoclips). This method is based on the principle that a regular cell centrifuge can be used to concentrate cells harvested from cell culture and then deposit the concentrated cell suspension to a slide evenly by using a cell spreader, followed by cell staining. The method presented is simple, rapid, economic, and efficient. This method may also avoid a possible change in cell morphology induced by cytocentrifuge.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/813216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65162306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Hyaluronan Synthase 3 Null Mice Exhibit Decreased Intestinal Inflammation and Tissue Damage in the DSS-Induced Colitis Model. 透明质酸合酶3缺失小鼠在dss诱导的结肠炎模型中表现出肠道炎症和组织损伤的减轻。

International Journal of Cell Biology Pub Date : 2015-01-01 Epub Date: 2015-09-10 DOI: 10.1155/2015/745237

Sean P Kessler, Dana R Obery, Carol de la Motte

{"title":"Hyaluronan Synthase 3 Null Mice Exhibit Decreased Intestinal Inflammation and Tissue Damage in the DSS-Induced Colitis Model.","authors":"Sean P Kessler, Dana R Obery, Carol de la Motte","doi":"10.1155/2015/745237","DOIUrl":"https://doi.org/10.1155/2015/745237","url":null,"abstract":"Hyaluronan (HA) overproduction is a hallmark of multiple inflammatory diseases, including inflammatory bowel disease (IBD). Hyaluronan can act as a leukocyte recruitment molecule and in the most common mouse model of intestinal inflammation, the chemically induced dextran sodium sulfate (DSS) experimental colitis model, we previously determined that changes in colon distribution of HA occur before inflammation. Therefore, we hypothesized that, during a pathologic challenge, HA promotes inflammation. In this study, we tested the progression of inflammation in mice null for the hyaluronan synthase genes (HAS1, HAS3, or both HAS1 and HAS3) in the DSS-colitis model. Our data demonstrate that both the HAS1/HAS3 double and the HAS3 null mice are protected from colitis, compared to wild-type and HAS1 null mice, as determined by measurement of weight loss, disease activity, serum IL-6 levels, histologic scoring, and immunohistochemistry. Most notable is the dramatic increase in submucosal microvasculature, hyaluronan deposition, and leukocyte infiltration in the inflamed colon tissue of wild-type and HAS1 null mice. Our data suggest, HAS3 plays a crucial role in driving gut inflammation. Developing a temporary targeted therapeutic intervention of HAS3 expression or function in the microcirculation may emerge as a desirable strategy toward tempering colitis in patients undergoing flares of IBD. ","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2015 ","pages":"745237"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/745237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34138712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery. 用于抗癌药物递送的羧甲基透明质酸稳定纳米颗粒。

International Journal of Cell Biology Pub Date : 2015-01-01 Epub Date: 2015-09-10 DOI: 10.1155/2015/249573

Jessica L Woodman, Min Sung Suh, Jianxing Zhang, Yuvabharath Kondaveeti, Diane J Burgess, Bruce A White, Glenn D Prestwich, Liisa T Kuhn

{"title":"Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery.","authors":"Jessica L Woodman, Min Sung Suh, Jianxing Zhang, Yuvabharath Kondaveeti, Diane J Burgess, Bruce A White, Glenn D Prestwich, Liisa T Kuhn","doi":"10.1155/2015/249573","DOIUrl":"https://doi.org/10.1155/2015/249573","url":null,"abstract":"Carboxymethyl hyaluronic acid (CMHA) is a semisynthetic derivative of HA that is recognized by HA binding proteins but contains an additional carboxylic acid on some of the 6-hydroxyl groups of the N-acetyl glucosamine sugar units. These studies tested the ability of CMHA to stabilize the formation of calcium phosphate nanoparticles and evaluated their potential to target therapy resistant, CD44(+)/CD24(-/low) human breast cancer cells (BT-474EMT). CMHA stabilized particles (nCaP(CMHA)) were loaded with the chemotherapy drug cis-diamminedichloroplatinum(II) (CDDP) to form nCaP(CMHA)CDDP. nCaP(CMHA)CDDP was determined to be poorly crystalline hydroxyapatite, 200 nm in diameter with a -43 mV zeta potential. nCaP(CMHA)CDDP exhibited a two-day burst release of CDDP that tapered resulting in 86% release by 7 days. Surface plasmon resonance showed that nCaP(CMHA)CDDP binds to CD44, but less effectively than CMHA or hyaluronan. nCaP(CMHA-AF488) was taken up by CD44(+)/CD24(-) BT-474EMT breast cancer cells within 18 hours. nCaP(CMHA)CDDP was as cytotoxic as free CDDP against the BT-474EMT cells. Subcutaneous BT-474EMT tumors were more reproducibly inhibited by a near tumor dose of 2.8 mg/kg CDDP than a 7 mg/kg dose nCaP(CMHA)CDDP. This was likely due to a lack of distribution of nCaP(CMHA)CDDP throughout the dense tumor tissue that limited drug diffusion. ","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2015 ","pages":"249573"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/249573","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34240812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12