International Journal of Cell Biology最新文献

{"title":"Exploring the Expanding Role of Nuclear Receptor LRH-1/NR5A2 in Cell Biology and Immunity.","authors":"Eleni Tsapalou, Panagiotis Michael, Maria Georgoula, Konstantinos Michail, Stamatia Papoutsopoulou, Barry J Campbell","doi":"10.1155/ijcb/3335387","DOIUrl":"10.1155/ijcb/3335387","url":null,"abstract":"<p><p>LRH-1/NR5A2 is a member of the NR5A subfamily of nuclear receptors, acting as a crucial transcription factor involved in diverse biological processes. Although LRH-1 has been extensively studied in vitro and in vivo, in relation to its role in embryonic development and differentiation and in adult tissue processes, such as cholesterol, glucose, and ethanol metabolism, current knowledge about its function in immunity is very limited. This review, therefore, presents the most recent knowledge regarding <i>NR5A2</i> regulation and LRH-1 function, including the wide spectrum of its target genes. In addition to LRH-1 regulating steroidogenesis in tissues such as the ovaries, testes, pancreas, and breast adipocytes, it is also key in driving glucocorticoid production in intestine, where its immunomodulatory activity is undoubtedly important in maintaining gut homeostasis and countering inflammation. Likewise, LRH-1 also appears to be crucial to the enterohepatic circulation, regulating hepatic bile acid synthesis and secretion, and intestinal transport/reabsorption. Our review also highlights fundamental roles for LRH-1 in macrophage polarization and in T cell homeostasis, revealing a major role in Th2 immune responses. Although no studies to date have explored the association of LRH-1 with respect to B-lymphocyte biology, we highlight the need for understanding its wider role in the immune system and its interplay with tissues and organs in health and disease.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2026 ","pages":"3335387"},"PeriodicalIF":0.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13643060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The HDAC Inhibitor Butyrate Reduces SOD1 Aggregation and Improves Motor Function in <i>C. elegans</i> and Cellular Models of ALS.","authors":"Fiona C Dresel, Martin Michaelis, Campbell W Gourlay","doi":"10.1155/ijcb/3022967","DOIUrl":"10.1155/ijcb/3022967","url":null,"abstract":"<p><p>Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterised by motor neuron loss and protein aggregation, commonly driven by mutations in superoxide dismutase 1 (Sod1). Recent evidence implicates gut microbiota-derived metabolites, such as butyrate, in modulating neurodegeneration, but the underlying mechanisms remain unclear. Here, we demonstrate that sodium butyrate (NaB), a histone deacetylase inhibitor and microbial metabolite, ameliorates ALS-related phenotypes in <i>C. elegans</i> and mammalian cell models expressing mutant isoforms of Sod1 linked to ALS. NaB treatment prevented Sod1 aggregation and restored motor function and axonal integrity in transgenic worms overexpressing Sod1^G85R. Mechanistically, NaB recapitulated the effects of the pan-HDAC inhibitor trichostatin A, suggesting HDAC inhibition as key to reducing Sod1 aggregation and its downstream effects. Application of NaB or the HDAC inhibitor valproic acid also prevented aggregation of Sod1^A4V, Sod1^G85R or Sod1^G37R in transfected human neuroblastoma cells. These findings support a conserved neuroprotective role for NaB and HDAC inhibitors via their antiaggregation activity. Our findings also verify <i>C. elegans</i> and neuroblastoma cell lines as excellent research tools to explore the mechanisms underlying the antiaggregation action of NaB and HDAC inhibitors, as well as their potential for future therapeutic development.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2026 ","pages":"3022967"},"PeriodicalIF":0.0,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13520122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148841551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Heme Oxygenase Contributes to Alleviate Salinity Damage in <i>Glycine max</i> L. Leaves\".","authors":"","doi":"10.1155/ijcb/9785268","DOIUrl":"https://doi.org/10.1155/ijcb/9785268","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2009/848516.].</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2026 ","pages":"9785268"},"PeriodicalIF":0.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Inhibition of TGF-<i>β</i>1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-<i>β</i>1-Induced EMT in Renal Fibrosis\".","authors":"","doi":"10.1155/ijcb/9758548","DOIUrl":"https://doi.org/10.1155/ijcb/9758548","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2019/9151394.].</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2026 ","pages":"9758548"},"PeriodicalIF":0.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13420739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148632208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Meta-Analysis and WGCNA Reveal Candidate Diagnostic Hub Genes in Clear Cell Carcinoma.","authors":"Haideh Namdari, Farhad Rezaei, Maryam Karamigolbaghi","doi":"10.1155/ijcb/5567255","DOIUrl":"10.1155/ijcb/5567255","url":null,"abstract":"<p><p>Clear cell renal cell carcinoma (ccRCC) remains poorly understood at the genetic level. In this study, 12 GEO microarray datasets were combined, and meta-analysis and multiparameter gene prioritization were used to reveal candidate diagnostic biomarkers. Improved preprocessing and analysis were used to identify differentially expressed genes (DEGs), which led to 4651 DEGs enriched in metabolic pathways, immune response, and kidney development. Key transcription factors (TFs), including ZNF692, ZNF395, and ZNF582, were identified. Utilizing WGCNA, 21 hub genes were identified, and diagnostic potential was determined via ROC analysis. Seventeen of the genes with AUC > 0.7 were validated as diagnostic hub genes, including ALDH2, ACADM, KIF11, and PTPRC. Nine of these genes showed significant prognostic relevance. The current work identifies key biomarkers that can enhance ccRCC diagnosis and guide future therapy.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2026 ","pages":"5567255"},"PeriodicalIF":0.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12865128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146120639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Important Aspects of siRNA Design for Optimal Efficacy In Vitro and In Vivo.","authors":"Mili S Bhakta-Yadav, Thomas L Brown","doi":"10.1155/ijcb/6663816","DOIUrl":"10.1155/ijcb/6663816","url":null,"abstract":"<p><p>siRNA is a versatile tool with practical applications in various fields, such as fundamental research, therapeutic development, and plant genetics. A few siRNA therapeutics have been FDA-approved, such as Onpattro (patisiran) and Leqvio (inclisiran) to treat hereditary transthyretin amyloidosis and primary hypercholesterolemia, respectively. In addition, several others are currently in clinical trials, highlighting the potential of siRNA-based treatment for undruggable targets. siRNA is a double-stranded RNA molecule that has the potential to inhibit gene expression by degrading target mRNA. The siRNA sequence must be precisely designed for effective gene knockdown and to minimize off-target effects. Strategies for designing siRNA to achieve optimal efficacy are presented in this review. We emphasize approaches that promote effective gene knockdown by siRNA. These approaches include preventing off-target RNAi and ensuring incorporation of the intended guide/antisense strand into RISC for targeted gene knockdown. This review also discusses the assessment of siRNA efficacy in vitro and the design of appropriate nonsilencing controls. Furthermore, the challenges of in vivo applications are identified, and strategies to overcome these challenges, such as siRNA delivery methods, biodistribution, and immunotoxicity prevention, are highlighted. Lastly, nucleotide chemical modifications to the ribose sugar and phosphodiester bonds and their effects on siRNA stability, activity, and interaction with the RISC complex are discussed. Overall, this review serves as a guide for well-designed and rigorously tested siRNA sequences, starting from initial in silico design to the application of siRNA for research or development of siRNA-based therapeutics.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2025 ","pages":"6663816"},"PeriodicalIF":0.0,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12752829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preservative Effectiveness of Lactic Acid Bacteria on Fruits and Vegetables.","authors":"Desalegn Amenu, Ayantu Nugusa, Temesgen Tafesse","doi":"10.1155/ijcb/5833236","DOIUrl":"10.1155/ijcb/5833236","url":null,"abstract":"<p><p><b>Background:</b> Lactic acid bacteria (LAB) are extensively used in the preservation of fruits and vegetables due to their ability to inhibit spoilage organisms and pathogenic bacteria. The fermentation process mediated by LAB not only extends the shelf life of produce but also enhances its nutritional and sensory properties. <b>Objective:</b> This study is aimed at reviewing the mechanisms through which LAB preserve fruits and vegetables, evaluating the benefits of their application, and highlighting recent advances in this field. <b>Methods:</b> A comprehensive literature review was conducted, focusing on the various preservative mechanisms of LAB, including acid production, bacteriocin secretion, competition for nutrients, and exopolysaccharide production. The impact of LAB on the shelf life, safety, nutritional value, and sensory attributes of fruits and vegetables was assessed through an analysis of recent scientific studies and case examples. <b>Results:</b> LAB extends the shelf life of fruits and vegetables primarily through the production of lactic acid, which lowers the pH and inhibits the growth of spoilage organisms and pathogens. The secretion of bacteriocins by LAB provides additional antimicrobial activity. Competition for nutrients further suppresses unwanted microbial growth. Exopolysaccharides produced by LAB can improve texture and form protective biofilms. Notable examples of LAB application include the fermentation of cabbage into sauerkraut, the production of kimchi, and the pickling of cucumbers. Advances in biotechnology have led to the development of optimized LAB strains and starter cultures, enhancing the efficacy of preservation processes. <b>Conclusion:</b> LAB are highly effective in preserving fruits and vegetables through multiple mechanisms, offering significant benefits in terms of extended shelf life, improved safety, enhanced nutritional value, and better sensory qualities. Ongoing research and technological advancements are expected to further improve the application of LAB in food preservation, making it a more efficient and widespread practice.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2025 ","pages":"5833236"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144800576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radioprotective Effects of Vitamin C, Cimetidine, and Famotidine on Lipid Peroxidase and Hepatic Glutathione Levels in Mouse Liver.","authors":"Mana Gholami, Ali Asghar Ahmadi, Reza Yusofvand, Milad Khanchoupan, Shima Hajimazdarany, Reza Najibi","doi":"10.1155/ijcb/1106920","DOIUrl":"10.1155/ijcb/1106920","url":null,"abstract":"<p><p>Radiation therapy is one of the most effective treatments for approximately 60% of patients with cancer. During radiation exposure, the overproduction of reactive oxygen species (ROS) disrupts the lipid layer of the membrane, leading to subsequent peroxide radical formation. Cimetidine (Cim) and famotidine (Fam) are histamine H2 receptor antagonists (H2 blocker), also known as peptic ulcer drugs, that exert radioprotective effects. Vitamin C (Vit.C) is an effective free radical and ROS scavenger with significant radioprotective effects. In this experimental study, male mice (6-8 weeks and 28 ± 3 g) were used in five groups. To evaluate ionizing radiation, gamma rays were used at two doses of 2 and 4 Gy and different doses of Cim, Fam, and Vit.C administered as the protectives. Finally, the livers of the mice were isolated and homogenized. The levels of lipid peroxidase and reduced and oxidized glutathione were measured using standard methods. With increasing radiation dose, lipid peroxidase activity, GSSG level, and glutathione content increased. The findings showed that in the drug-only group, Vit.C had better protection than the other two drugs, and the combination of the three drugs had excellent radiation protection. Radiation protection of normal cells in radiotherapy is a valuable necessity. A number of drugs can protect cells against ionizing radiation through different mechanisms. The results suggest that Fam, Cim, and Vit.C can be radioprotective individually or in combination.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2025 ","pages":"1106920"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11724733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study on the Effects of Mesenchymal Stem Cells and Their Conditioned Medium on Caco-2 Cells as an In Vitro Model for Inflammatory Bowel Disease.","authors":"Naser Kalhor, Hoda Fazaeli, Faezeh Davoodi Asl, Azar Sheikholeslami, Seyed Jalal Eshagh Hoseini, Mohsen Sheykhhasan","doi":"10.1155/2024/1022338","DOIUrl":"https://doi.org/10.1155/2024/1022338","url":null,"abstract":"<p><p>Recent research indicates that mesenchymal stem cells (MSCs), known for their anti-inflammatory and anti-infectious properties, could be a promising alternative for treating inflammatory diseases such as inflammatory bowel disease (IBD). This study examined how MSCs and their derivatives, when cocultured with interleukin-1<i>β</i> (IL-1<i>β</i>)-stimulated Caco-2 cells, affect the expression of genes related to inflammation, microbes, and apoptosis. In the experiment, Caco-2 cells were exposed to 10 ng/mL of IL-1<i>β</i> for 24 h. MSCs were sourced from human bone marrow, adipose tissue (AD-MSC), and menstrual blood. These MSCs and their conditioned medium (CM) were then cocultured with the IL-1<i>β</i>-induced Caco-2 cells. After 48 h, gene expression levels were analyzed using real-time PCR, and the data were statistically evaluated using <i>T</i>-tests, <i>U</i>-Mann-Whitney, and Tukey's post hoc analyses. The results indicated that IL-1<i>β</i> at 10 ng/mL was the optimal concentration for inducing Caco-2 cells with the highest viability and minimal damage. Among the MSCs tested, AD-MSCs were the most effective in regulating gene expression. Specifically, AD-MSC treatment significantly reduced the mRNA expression of TNF-<i>α</i> and IL-1<i>β</i>, both of which are crucial in sustaining inflammatory responses (<i>p</i> ≤ 0.05). This study concludes that AD-MSCs have superior effects compared to other MSC sources in modulating genes associated with inflammation, antibacterial effects, and apoptosis in an in vitro model of IBD using Caco-2 cells.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2024 ","pages":"1022338"},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Exposure to Mobile Phones on Electrical Cardiac Measurements: A Multivariate Analysis and a Variable Selection Algorithm to Detect the Relationship With Mean Changes.","authors":"Nader Alharbi, Mohammed Alassiri","doi":"10.1155/2024/7093771","DOIUrl":"10.1155/2024/7093771","url":null,"abstract":"<p><p><b>Background:</b> The exponential growth in mobile phone usage has raised concerns about electromagnetic field (EMF) exposure and its health risks. Blood pressure and BMI, which impair heart function due to decreased adrenoreceptor responsiveness, parasympathetic tone withdrawal, and increased sympathetic activity, may further exacerbate these risks. However, the effects of radiofrequency electromagnetic (RF-EM) exposure from mobile phones on electrocardiograms (ECGs) and heart rate variability (HRV) in individuals remain unclear. <b>Purpose:</b> Building upon our previous findings on HRV changes due to mobile phone proximity, this study is aimed at significantly enhancing the analytical approach used to assess the effects of mobile phones on cardiac parameters. This study exploits data from a previous study but with a different purpose. The aim of this study is twofold: (a) to examine whether exposure to mobile phones changes the five variables (P-R, QRS, QT, ST, and HR) in a multivariate manner and (b) to examine whether the blood pressure and/or the body mass index (BMI), which acts as a proxy for obesity, have an effect on the change of these five variables. For both aspects of the study, four cycles are performed. <b>Method:</b> We conducted multivariate analysis on previously collected electrical cardiac measurement data from 20 healthy male subjects exposed to mobile phone EMF, with the mobile phones placed at four different body locations. The one-sample Hotelling <i>T</i> ² test on the mean vector of differences was utilised instead of multiple paired <i>t</i>-tests. This multivariate method comprehensively analyzes data features and accounts for variable correlations, unlike multiple univariate analyses. Given our small sample size, we employed the MMPC variable selection algorithm to identify predictor variables significantly related to mean changes. <b>Results:</b> Significant alterations in ECG intervals and heart rate were noted in the subjects before and after the first EMF exposure cycle, independent of their BMI. Notably, heart rate, P-R, and QRS intervals fell postexposure while QT and ST intervals increased. These changes were influenced by variations in systolic blood pressure, with BMI showing no significant effect. <b>Conclusion:</b> The observed modifications in cardiac electrical measurements due to mobile phone EMF exposure are attributed to the effects of EMF itself, with no impact from BMI on the extent of these changes.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2024 ","pages":"7093771"},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}