Anne Meinzinger, Áron Zsigmond, Péter Horváth, Alexandra Kellenberger, Katalin Paréj, Tiziano Tallone, Beáta Flachner, Marcell Cserhalmi, Zsolt Lőrincz, Sándor Cseh, Doron Shmerling
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引用次数: 0
Abstract
Inducible gene regulation methods are indispensable in diverse biological applications, yet many of them have severe limitations in their applicability. These include inducer toxicity, a limited variety of organisms the given system can be used in, and side effects of the induction method. In this study, a novel inducible system, the RuX system, was created using a mutant ligand-binding domain of the glucocorticoid receptor (CS1/CD), used together with various genetic elements such as the Gal4 DNA-binding domain or Cre recombinase. The RuX system is shown to be capable of over 1000-fold inducibility, has flexible applications, and is offered for use in cell cultures.
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