International Journal of Cell Biology最新文献

{"title":"Inhibition of TGF-<i>β</i>1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-<i>β</i>1-Induced EMT in Renal Fibrosis.","authors":"Aurore Devocelle, Lola Lecru, Hélène François, Christophe Desterke, Cindy Gallerne, Pierre Eid, Oberlin Estelle, Bruno Azzarone, Julien Giron-Michel","doi":"10.1155/2019/9151394","DOIUrl":"10.1155/2019/9151394","url":null,"abstract":"<p><p>Renal tubulointerstitial fibrosis is the final common pathway in end-stage renal disease and is characterized by aberrant accumulation of extracellular matrix (ECM) components secreted by myofibroblasts. Tubular type 2 EMT, induced by TGF-<i>β</i>, plays an important role in renal fibrosis, by participating directly or indirectly in myofibroblasts generation. TGF-<i>β</i>1-induced apoptosis and fibrosis in experimental chronic murine kidney diseases are concomitantly associated with an intrarenal decreased expression of the IL-15 survival factor. Since IL-15 counteracts TGF-<i>β</i>1 effects in different cell models, we analyzed whether (1) human chronic inflammatory nephropathies evolving towards fibrosis could be also characterized by a weak intrarenal IL-15 expression and (2) IL-15 could inhibit epithelial-mesenchymal transition (EMT) and excess matrix deposition in human renal proximal tubular epithelial cells (RPTEC). Our data show that different human chronic kidney diseases are characterized by a strong decreased expression of intrarenal IL-15, which is particularly relevant in diabetic nephropathy, in which type 2 tubular EMT plays an important role in fibrosis. Moreover, primary epithelial tubular cultures deprived of growth supplements rapidly produce active TGF-<i>β</i>1 inducing a \"spontaneous\" EMT process characterized by the loss of membrane-bound IL-15 (mbIL-15) expression. Both \"spontaneous\" EMT and recombinant human (rh) TGF-<i>β</i>1-induced EMT models can be inhibited by treating RPTEC and HK2 cells with rhIL-15. Through a long-lasting phospho-c-jun activation, IL-15 inhibits rhTGF-<i>β</i>1-induced Snail1 expression, the master inducer of EMT, and blocks TGF-<i>β</i>1-induced tubular EMT and downstream collagen synthesis. In conclusion, our data suggest that intrarenal IL-15 could be a natural inhibitor of TGF-<i>β</i> in human kidney able to guarantee epithelial homeostasis and to prevent EMT process. Thus, both <i>in vivo</i> and <i>in vitro</i> an unbalance in intrarenal IL-15 and TGF-<i>β</i>1 levels could render RPTEC cells more prone to undergo EMT process. Exogenous IL-15 treatment could be beneficial in some human nephropathies such as diabetic nephropathy.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2019 1","pages":"9151394"},"PeriodicalIF":0.0,"publicationDate":"2019-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46403905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering of L-Plastin Peptide-Loaded Biodegradable Nanoparticles for Sustained Delivery and Suppression of Osteoclast Function In Vitro.","authors":"Sunipa Majumdar, Aniket S Wadajkar, Hanan Aljohani, Mark A Reynolds, Anthony J Kim, Meenakshi Chellaiah","doi":"10.1155/2019/6943986","DOIUrl":"10.1155/2019/6943986","url":null,"abstract":"<p><p>We have recently demonstrated that a small molecular weight amino-terminal peptide of L-plastin (10 amino acids; \"MARGSVSDEE\") suppressed the phosphorylation of endogenous L-plastin. Therefore, the formation of nascent sealing zones (NSZs) and bone resorption are reduced. The aim of this study was to develop a biodegradable and biocompatible PLGA nanocarrier that could be loaded with the L-plastin peptide of interest and determine the efficacy <i>in vitro</i> in osteoclast cultures. L-plastin MARGSVSDEE (P1) and scrambled control (P3) peptide-loaded PLGA-PEG nanoparticles (NP1 and NP3, respectively) were synthesized by double emulsion technique. The biological effect of nanoparticles on osteoclasts was evaluated by immunoprecipitation, immunoblotting, rhodamine-phalloidin staining of actin filaments, and pit forming assays. Physical characterization of well-dispersed NP1 and NP3 demonstrated ~130-150 nm size, < 0.07 polydispersity index, ~-3 mV <i>ζ</i>-potential, and a sustained release of the peptide for three weeks. Biological characterization in osteoclast cultures demonstrated the following: NP1 significantly reduced (a) endogenous L-plastin phosphorylation; (b) formation of NSZs and sealing rings; (c) resorption. However, the assembly of podosomes which are critical for cell adhesion was not affected. L-plastin peptide-loaded PLGA-PEG nanocarriers have promising potential for the treatment of diseases associated with bone loss. Future studies will use this sustained release of peptide strategy to systematically suppress osteoclast bone resorption activity <i>in vivo</i> in mouse models demonstrating bone loss.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2019 ","pages":"6943986"},"PeriodicalIF":0.0,"publicationDate":"2019-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37328972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Targeting HIF-1<i>α</i> to Prevent Renal Ischemia-Reperfusion Injury: Does It Work?\"","authors":"Kapil Sethi,&nbsp;Kenny Rao,&nbsp;Arthur Shulkes,&nbsp;Graham Baldwin,&nbsp;Damien Bolton,&nbsp;Oneel Patel,&nbsp;Joseph Ischia","doi":"10.1155/2019/9598038","DOIUrl":"https://doi.org/10.1155/2019/9598038","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2018/9852791.].</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2019 ","pages":"9598038"},"PeriodicalIF":0.0,"publicationDate":"2019-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2019/9598038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37244782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro.","authors":"Syed Ahmad Tajudin T-Johari,&nbsp;Fatimah Hashim,&nbsp;Wan Iryani Ismail,&nbsp;Abdul Manaf Ali","doi":"10.1155/2019/3059687","DOIUrl":"https://doi.org/10.1155/2019/3059687","url":null,"abstract":"<p><p>Combination of natural products with chemodrugs is becoming a trend in discovering new therapeutics approach for enhancing the cancer treatment process. In the present study, we aimed to investigate the cytotoxic and apoptosis induction of Gelam honey (GH) combined with or without 5-Fluorouracil (5-FU) on HT-29 cells. The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to assess cytotoxicity. Morphological changes and apoptosis were determined by the inverted microscope, Annexin V-FITC, and DNA fragmentation via flow cytometric analysis, respectively. Our results demonstrate that combined treatment revealed a remarkable and concentration-dependent cytotoxic effect on HT-29 cells in comparison with GH and 5-FU alone. Flow cytometry analysis showed that early apoptosis event was more pronounced in combined treatment. In addition, compared to 5-FU alone, apoptosis of HT-29 cells treated with combinations of GH and 5-FU demonstrated increasing percentages of fragmented DNA. Our results suggest that GH has a synergistic cytotoxic effect with 5-FU in HT-29 cell lines <i>in vitro</i>. Although the actions of the molecular mechanisms are not yet clear, the results reveal that the combination of GH and 5-FU could have the potential as a therapeutic agent.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2019 ","pages":"3059687"},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2019/3059687","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37100436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.","authors":"Masoumeh Rostami,&nbsp;Kamran Haidari,&nbsp;Majid Shahbazi","doi":"10.1155/2018/8213912","DOIUrl":"https://doi.org/10.1155/2018/8213912","url":null,"abstract":"<p><p>The immunomodulatory and self-renewable features of human adipose mesenchymal stem cells (hAD-MSCs) mark their importance in regenerative medicine. Interleukin 23 (IL- 23) as a proinflammatory cytokine suppresses T regulatory cells (Treg) and promotes the response of T helper 17 (Th17) and T helper 1 (Th1) cells. This pathway starts inflammation and immunosuppression in several autoimmune diseases. The current study for producing recombinant IL- 23 decoy receptor (RIL- 23R) using hAD-MSCs as a good candidate for ex vivo cell-based gene therapy purposes reducing inflammation in autoimmune diseases. hAD-MSCs was isolated from lipoaspirate and then characterized by differentiation. RIL- 23R was designed and cloned into a pCDH-813A- 1 lentiviral vector. The transduction of hAD-MSCs was performed at MOI (multiplicity of infection) = 50 with pCDH- EFI <i>α</i>- RIL- 23R- PGK copGFP. Expressions of RIL- 23R and octamer-binding transcription factor 4 (OCT- 4) were determined by real-time polymerase chain reaction (real time-PCR). Self-renewing properties were assayed with OCT- 4. Bioactivity of the designed RIL- 23R was evaluated by IL- 17 and IL- 10 expression of mouse splenocytes. Cell differentiation confirmed the true isolation of hAD-MSCs from lipoaspirate. Restriction of the enzyme digestion and sequencing verified the successful cloning of RIL- 23R in the CD813A-1 lentiviral vector. The green fluorescent protein (GFP) positive transduction rate was up to 90%, and real-time PCR showed the expression level of RIL-23R. Oct-4 had a similar expression pattern with nontransduced hAD-MSCs and transduced hAD-MSCs/ RIL-23R indicating that lentiviral vector did not affect hAD-MSCs characteristics. Downregulation of IL-17 and upregulation of IL-10 showed the correct activity of the engineered hAD-MSCs. The results showed that the transduced hAD-MSCs/ RIL- 23R, expressing IL-23 decoy receptor, can give a useful approach for a basic research on cell-based gene therapy for autoimmune disorders.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2018 ","pages":"8213912"},"PeriodicalIF":0.0,"publicationDate":"2018-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/8213912","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36924105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting HIF-1<i>α</i> to Prevent Renal Ischemia-Reperfusion Injury: Does It Work?","authors":"Kapil Sethi,&nbsp;Kenny Rao,&nbsp;Damien Bolton,&nbsp;Oneel Patel,&nbsp;Joseph Ischia","doi":"10.1155/2018/9852791","DOIUrl":"10.1155/2018/9852791","url":null,"abstract":"<p><p>Partial nephrectomy (open or minimally invasive) usually requires temporary renal arterial occlusion to limit intraoperative bleeding and improve access to intrarenal structures. This is a time-critical step due to the critical ischemia period of renal tissue. Prolonged renal ischemia may lead to irreversible nephron damage in the remaining tissue and, ultimately, chronic kidney disease. This is potentiated by the incompletely understood ischemia-reperfusion injury (IRI). A key mechanism in IRI prevention appears to be the upregulation of an intracellular transcription protein, Hypoxia-Inducible Factor (HIF). HIF mediates metabolic adaptation, angiogenesis, erythropoiesis, cell growth, survival, and apoptosis. Upregulating HIF-1<i>α</i> via ischemic preconditioning (IPC) or drugs that simulate hypoxia (hypoxia-mimetics) has been investigated as a method to reduce IRI. While many promising chemical agents have been trialed for the prevention of IRI in small animal studies, all have failed in human trials. The aim of this review is to highlight the techniques and drugs that target HIF-1<i>α</i> and ameliorate IRI associated with renal ischemia. Developing a technique or drug that could reduce the risk of acute kidney injury associated with renal IRI would have an immediate worldwide impact on multisystem surgeries that would otherwise risk ischemic tissue injury.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2018 ","pages":"9852791"},"PeriodicalIF":0.0,"publicationDate":"2018-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/9852791","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36866348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Grammatophyllum speciosum</i> Ethanolic Extract Promotes Wound Healing in Human Primary Fibroblast Cells.","authors":"Saraporn Harikarnpakdee,&nbsp;Verisa Chowjarean","doi":"10.1155/2018/7836869","DOIUrl":"https://doi.org/10.1155/2018/7836869","url":null,"abstract":"<p><p><i>Grammatophyllum speciosum</i> is a plant in Orchidaceae family which contains a variety of phytochemical compounds that might be beneficial for medicinal use. This study aimed to evaluate the activity of pseudobulb of <i>G. speciosum</i> extract (GSE) in wound healing processes in human primary fibroblast cells along with <i>in vitro</i> antioxidant activity and total phenolic content of GSE. Scratch wound healing assay indicated that GSE was capable of increasing migration rate after 6 and 9 hours of treatment. Besides, the extract was able to scavenge DPPH, ABTS, and superoxide anion radicals indicating the antioxidative property of GSE. This study suggested a novel role of the of pseudobulb extract of <i>G. speciosum</i> as a wound healing enhancer. The results from this study might be beneficial for the development of further novel active compounds for skin wound healing.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2018 ","pages":"7836869"},"PeriodicalIF":0.0,"publicationDate":"2018-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/7836869","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36658867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Seipin: From Biochemistry to Bioinformatics Predictions.","authors":"Aquiles Sales Craveiro Sarmento,&nbsp;Lázaro Batista de Azevedo Medeiros,&nbsp;Lucymara Fassarella Agnez-Lima,&nbsp;Josivan Gomes Lima,&nbsp;Julliane Tamara Araújo de Melo Campos","doi":"10.1155/2018/5207608","DOIUrl":"https://doi.org/10.1155/2018/5207608","url":null,"abstract":"<p><p>Seipin is a nonenzymatic protein encoded by the <i>BSCL2</i> gene. It is involved in lipodystrophy and seipinopathy diseases. Named in 2001, all seipin functions are still far from being understood. Therefore, we reviewed much of the research, trying to find a pattern that could explain commonly observed features of seipin expression disorders. Likewise, this review shows how this protein seems to have tissue-specific functions. In an integrative view, we conclude by proposing a theoretical model to explain how seipin might be involved in the triacylglycerol synthesis pathway.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2018 ","pages":"5207608"},"PeriodicalIF":0.0,"publicationDate":"2018-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/5207608","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36653923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Kinetic Modeling of the Mitochondrial Energy Metabolism of Neuronal Cells: The Impact of Reduced α-Ketoglutarate Dehydrogenase Activities on ATP Production and Generation of Reactive Oxygen Species\".","authors":"Nikolaus Berndt,&nbsp;Sascha Bulik,&nbsp;Hermann-Georg Holzhütter","doi":"10.1155/2018/6139262","DOIUrl":"https://doi.org/10.1155/2018/6139262","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2012/757594.].</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2018 ","pages":"6139262"},"PeriodicalIF":0.0,"publicationDate":"2018-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/6139262","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36539284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic Contribution of High-Mobility Group A Proteins to Stem Cell Properties.","authors":"Vincenzo Giancotti, Natascha Bergamin, Palmina Cataldi, Claudio Rizzi","doi":"10.1155/2018/3698078","DOIUrl":"10.1155/2018/3698078","url":null,"abstract":"<p><p>High-mobility group A (HMGA) proteins have been examined to understand their participation as structural epigenetic chromatin factors that confer stem-like properties to embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and cancer stem cells (CSCs). The function of HMGA was evaluated in conjunction with that of other epigenetic factors such as histones and microRNAs (miRs), taking into consideration the posttranscriptional modifications (PTMs) of histones (acetylation and methylation) and DNA methylation. HMGA proteins were coordinated or associated with histone and DNA modification and the expression of the factors related to pluripotency. CSCs showed remarkable differences compared with ESCs and iPSCs.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2018 ","pages":"3698078"},"PeriodicalIF":0.0,"publicationDate":"2018-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3698078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36182782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}