Biomolecular Concepts最新文献_第9页

Calcium Dynamics Regulate Protective Responses and Growth of Staphylococcus aureus in Macrophages. 钙动力学调节巨噬细胞的保护反应和金黄色葡萄球菌的生长。

Biomolecular Concepts Pub Date : 2020-12-31 DOI: 10.1515/bmc-2020-0021

Chaitenya Verma, Kumar Rana Ankush, Vandana Anang, Brijendra K Tiwari, Aayushi Singh, Shakuntala Surender Kumar Saraswati, Malini Shariff, Krishnamurthy Natarajan

{"title":"Calcium Dynamics Regulate Protective Responses and Growth of Staphylococcus aureus in Macrophages.","authors":"Chaitenya Verma, Kumar Rana Ankush, Vandana Anang, Brijendra K Tiwari, Aayushi Singh, Shakuntala Surender Kumar Saraswati, Malini Shariff, Krishnamurthy Natarajan","doi":"10.1515/bmc-2020-0021","DOIUrl":"https://doi.org/10.1515/bmc-2020-0021","url":null,"abstract":"Staphylococcus aureus (S. aureus) is a gram-positive bacteria, which causes various fatal respiratory infections including pneumonia. The emergence of Methicillin-Resistance Staphylococcus aureus (MRSA) demands a thorough understanding of host-pathogen interactions. Here we report the role of calcium in regulating defence responses of S. aureus in macrophages. Regulating calcium fluxes in cells by different routes differentially governs the expression of T cell costimulatory molecule CD80 and Th1 promoting IL-12 receptor. Inhibiting calcium influx from extracellular medium increased expression of IFN-γ and IL-10 while blocking calcium release from the intracellular stores inhibited TGF-β levels. Blocking voltage-gated calcium channels (VGCC) inhibited the expression of multiple cytokines. While VGCC regulated the expression of apoptosis protein Bax, extracellular calcium-regulated the expression of Cytochrome-C. Similarly, VGCC regulated the expression of autophagy initiator Beclin-1. Blocking VGCC or calcium release from intracellular stores promoted phagosome-lysosome fusion, while activating VGCC inhibited phagosomelysosome fusion. Finally, calcium homeostasis regulated intracellular growth of Staphylococcus, although using different mechanisms. While blocking extracellular calcium influx seems to rely on IFN-γ and IL-12Rβ receptor mediated reduction in bacterial survival, blocking either intracellular calcium release or via VGCC route seem to rely on enhanced autophagy mediated reduction of intracellular bacterial survival. These results point to fine-tuning of defence responses by routes of calcium homeostasis.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"230-239"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2020-0021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25494136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Role of phase partitioning in coordinating DNA damage response: focus on the Apurinic Apyrimidinic Endonuclease 1 interactome. 相分配在协调DNA损伤反应中的作用:聚焦于无嘌呤嘧啶内切酶1相互作用组。

Biomolecular Concepts Pub Date : 2020-12-23 DOI: 10.1515/bmc-2020-0019

Damiano Tosolini, Giulia Antoniali, Emiliano Dalla, Gianluca Tell

{"title":"Role of phase partitioning in coordinating DNA damage response: focus on the Apurinic Apyrimidinic Endonuclease 1 interactome.","authors":"Damiano Tosolini, Giulia Antoniali, Emiliano Dalla, Gianluca Tell","doi":"10.1515/bmc-2020-0019","DOIUrl":"https://doi.org/10.1515/bmc-2020-0019","url":null,"abstract":"Liquid-liquid phase separation (LLPS) is a way to concentrate biochemical reactions while excluding noninteracting components. Disordered domains of proteins, as well as interaction with RNA, favor condensation but are not mandatory for modulating this process. Recent insights about phase-separation mechanisms pointed to new fascinating models that could explain how cells could cope with DNA damage responses, conferring both spatial and temporal fine regulation. APE1 is a multifunctional protein belonging to the Base Excision Repair (BER) pathway, bearing additional 'non-canonical' DNA-repair functions associated with processes like RNA metabolism. Recently, it has been highlighted that several DNA repair enzymes, such as 53BP1 and APE1, are endowed with RNA binding abilities. In this work, after reviewing the recent literature supporting a role of LLPS in DDR, we analyze, as a proof of principle, the interactome of APE1 using a bioinformatics approach to look for clues of LLPS in BER. Some of the APE1 interactors are associated with cellular processes in which LLPS has been either proved or proposed and are involved in different pathogenic events. This work might represent a paradigmatical pipeline for evaluating the relevance of LLPS in DDR.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"209-220"},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2020-0019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39161852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Alzheimer's disease: exploring nature's 'medicinal chest' for new therapeutic agents. 阿尔茨海默病:探索大自然的“药箱”，寻找新的治疗药物。

Biomolecular Concepts Pub Date : 2020-12-23 DOI: 10.1515/bmc-2020-0018

Anthony Tsarbopoulos

{"title":"Alzheimer's disease: exploring nature's 'medicinal chest' for new therapeutic agents.","authors":"Anthony Tsarbopoulos","doi":"10.1515/bmc-2020-0018","DOIUrl":"https://doi.org/10.1515/bmc-2020-0018","url":null,"abstract":"Natural products have served humanity as a valuable source for the discovery and development of therapeutic agents. In addition, these phytochemicals can function as lead compounds for the development of synthetic analogs aimed at treating human diseases. In our aging society, Alzheimer's disease (AD) is the most common cause of dementia, which is characterized by a significant and progressive loss of memory and other cognitive functions. As society demographics change, the predominance of AD and other age-related dementias is increasing, with concurrent financial and societal costs.AD represents one of the most remarkable scientific challenges for drug discovery as the search for effective disease-modifying agents has been unsuccessful. Medicinal plants have been used for their \"anti-aging\" properties, and cognitive enhancing properties. In the past decades, natural products have been studied for their anti-AD properties, and their potential for developing therapeutic agents against several molecular targets has been evaluated. This insight evaluates the prospects of medicinal plants for providing disease-modifying, as well as disease-preventing, agents for AD.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"201-208"},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2020-0018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39161851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Influence of photobiomodulation and vitamin D on osteoblastic differentiation of human periodontal ligament stem cells and bone-like tissue formation through enzymatic activity and gene expression. 光生物调节和维生素D通过酶活性和基因表达对人牙周韧带干细胞成骨分化和骨样组织形成的影响。

Biomolecular Concepts Pub Date : 2020-12-23 DOI: 10.1515/bmc-2020-0016

Latifa Mohamed Abdelgawad, Asmaa Mohamed Abdelaziz, Dina Sabry, Marwa Abdelgwad

{"title":"Influence of photobiomodulation and vitamin D on osteoblastic differentiation of human periodontal ligament stem cells and bone-like tissue formation through enzymatic activity and gene expression.","authors":"Latifa Mohamed Abdelgawad, Asmaa Mohamed Abdelaziz, Dina Sabry, Marwa Abdelgwad","doi":"10.1515/bmc-2020-0016","DOIUrl":"https://doi.org/10.1515/bmc-2020-0016","url":null,"abstract":"Background: (1)Human periodontal ligament stem cells (HPDLSCs) are a unique population of mesenchymal stem cells (MSCs). Recently, the positive effects of photobiomodulation on the regulation of MSCs proliferation and osteogenic differentiation have gained significant attention. This study aimed to assess the effects of photobiomodulation and vitamin D (as an anabolic factor) on HPDLSCs for bone regeneration.Methods: (2)HPDLSCs were collected, isolated, and characterized and then divided into six groups: groups I and II, control and (10-7 Mol) vitamin D, respectively; group III, irradiation at 1 J/cm2 of 808-nm diode laser; group IV, irradiation at 1 J/cm2 and culture with vitamin D; group V, irradiation at 2 J/cm2, and group VI, irradiation at 2 J/cm2 and culture with vitamin D. Cell viability assay was measured through MTT assay and cell growth curve. Alkaline phosphatase (ALP) enzyme activity and mRNA levels of RUNX2, collagen 1 (Col-1), ALP, and osteonectin were also assessed.Results: (3)Photobiomodulation at 1 and 2 J/cm2 combined with vitamin D significantly promoted HPDLSC proliferation (in MTT assay and cell growth curve results) and osteogenic differentiation (through the gene expression of RUNX2, Col-1, ALP, and osteonectin levels (p < 0.05).Conclusion: (4)Laser irradiation at 2 J/cm2 combined with vitamin D3 enhanced osteoblast differentiation and proliferation of cultured HPDLSCs and thus could further substitute bone grafting.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"172-181"},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2020-0016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39161849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid. 基于质谱技术对从卵泡液中分离出的总 IgG 和总蛋白质组 N-糖进行糖谱分析。

Biomolecular Concepts Pub Date : 2020-10-25 DOI: 10.1515/bmc-2020-0015

Marko Klobučar, Sanja Dević Pavlić, Iris Car, Neda Smiljan Severinski, Tamara Tramišak Milaković, Anđelka Radojčić Badovinac, Sandra Kraljević Pavelić

{"title":"Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid.","authors":"Marko Klobučar, Sanja Dević Pavlić, Iris Car, Neda Smiljan Severinski, Tamara Tramišak Milaković, Anđelka Radojčić Badovinac, Sandra Kraljević Pavelić","doi":"10.1515/bmc-2020-0015","DOIUrl":"10.1515/bmc-2020-0015","url":null,"abstract":"Couples with infertility issues have been assisted by in vitro fertilization reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro fertilization procedures, but other processes are also critical for fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing fertilization through either (1) assisted reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"153-171"},"PeriodicalIF":0.0,"publicationDate":"2020-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10390847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Conceptual Model for the Dual Role of FOF1-ATP Synthase in Cell Life and Cell Death. FOF1-ATP 合成酶在细胞生命和细胞死亡中双重作用的新概念模型。

Biomolecular Concepts Pub Date : 2020-08-22 DOI: 10.1515/bmc-2020-0014

Sunil Nath

{"title":"A Novel Conceptual Model for the Dual Role of FOF1-ATP Synthase in Cell Life and Cell Death.","authors":"Sunil Nath","doi":"10.1515/bmc-2020-0014","DOIUrl":"10.1515/bmc-2020-0014","url":null,"abstract":"The mitochondrial permeability transition (MPT) has been one of the longstanding enigmas in biology. Its cause is currently at the center of an extensive scientific debate, and several hypotheses on its molecular nature have been put forward. The present view holds that the transition arises from the opening of a high-conductance channel in the energy-transducing membrane, the permeability transition pore (PTP), also called the mitochondrial megachannel or the multiconductance channel (MMC). Here, the novel hypothesis is proposed that the aqueous access channels at the interface of the c-ring and the a-subunit of FO in the FOF1-ATP synthase are repurposed during induction of apoptosis and constitute the elusive PTP/ MMC. A unifying principle based on regulation by local potentials is advanced to rationalize the action of the myriad structurally and chemically diverse inducers and inhibitors of PTP/MMC. Experimental evidence in favor of the hypothesis and its differences from current models of PTP/MMC are summarized. The hypothesis explains in considerable detail how the binding of Ca2+ to a β-catalytic site (site 3) in the F1 portion of ATP synthase triggers the opening of the PTP/MMC. It is also shown to connect to longstanding proposals within Nath's torsional mechanism of energy transduction and ATP synthesis as to how the binding of MgADP to site 3 does not induce PTP/MMC, but instead catalyzes physiological ATP synthesis in cell life. In the author's knowledge, this is the first model that explains how Ca2+ transforms the FOF1-ATP synthase from an exquisite energy-conserving enzyme in cell life into an energy-dissipating structure that promotes cell death. This has major implications for basic as well as for clinical research, such as for the development of drugs that target the MPT, given the established role of PTP/MMC dysregulation in cancer, ischemia, cardiac hypertrophy, and various neurodegenerative diseases.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"143-152"},"PeriodicalIF":0.0,"publicationDate":"2020-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10071424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Endothelin-3 and Multiple Sclerosis. 对内皮素-3 和多发性硬化症的认识

Biomolecular Concepts Pub Date : 2020-06-25 DOI: 10.1515/bmc-2020-0012

Lucia Monti, Umberto Arrigucci, Alessandro Rossi

引用次数: 0

Erratum to "Polymorphism of MMP1 and MMP3 promoter regions and HR-HPV infection in women from Burkina Faso and Côte d'Ivoire". 对“MMP1和MMP3启动子区域多态性与布基纳法索和Côte科特迪瓦妇女HR-HPV感染”的勘误。

Biomolecular Concepts Pub Date : 2020-06-20 DOI: 10.1515/bmc-2020-0013

Prosper Bado, Florencia Wendkuuni Djigma, Théodora Mahoukèdè Zohoncon, Dorcas Obiri-Yeboah, Esther Mah Alima Traoré, Abdoul Karim Ouattara, Teega-Wendé Clarisse Ouedraogo, Shoukrat Ohuwa Toyin Bello, Marius Ayaovi Setor, Ina Marie Angèle Traore, Apollinaire Horo, Kouame Privat Kouakou, Théophane Albert Yonli, Charlemagne Ouedraogo, Jacques Simpore

引用次数: 1

Genotypic distribution of human oncogenic papillomaviruses in sexually active women in Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions. 布基纳法索性活跃妇女中人类致癌乳头瘤病毒的基因型分布：中部、中东部和上盆地地区。

Biomolecular Concepts Pub Date : 2020-05-17 DOI: 10.1515/bmc-2020-0011

Rogomenoma Alice Ouedraogo, Théodora Mahoukèdè Zohoncon, Ina Marie Angèle Traore, Abdoul Karim Ouattara, Sindimalgdé Patricia Guigma, Florencia Wendkuuni Djigma, Dorcas Obiri-Yeboah, Charlemagne Ouedraogo, Jacques Simpore

{"title":"Genotypic distribution of human oncogenic papillomaviruses in sexually active women in Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions.","authors":"Rogomenoma Alice Ouedraogo, Théodora Mahoukèdè Zohoncon, Ina Marie Angèle Traore, Abdoul Karim Ouattara, Sindimalgdé Patricia Guigma, Florencia Wendkuuni Djigma, Dorcas Obiri-Yeboah, Charlemagne Ouedraogo, Jacques Simpore","doi":"10.1515/bmc-2020-0011","DOIUrl":"10.1515/bmc-2020-0011","url":null,"abstract":"Objective this study was conducted to determine the distribution of high-risk human papillomavirus (HR-HPV) genotypes in women in the general population of three regions of Burkina Faso. Method This multicenter, descriptive cross-sectional study involved 1321 sexually active women in five cities in three regions of Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions. After collection of endocervical specimens, pre-cervical lesions were screened by visual inspection with acetic acid and lugol (VIA / VILI). HR-HPV genotypes were characterized by multiplex real-time PCR after extraction of viral DNA. Results The mean age of women was 31.98 ± 10.09 years. The HR-HPV infection in the three regions ranged from 26.16% to 43.26% with 35.42% as overall prevalence in women. The most common HR-HPV genotypes in descending order were: HPV 56, 52, 66, 59, 39, 51, 18, 35. The prevalence of bivalent vaccine genotypes (HPV16 / 18) was 7.83% against 63.78% of genotypes not covered by HPV vaccine; 36.32% (170/468) of women had multiple concomitant HR-HPV infections. Conclusion this study showed significant regional variation and high prevalence of HR-HPV infection in women. The predominant genotypes differ from those covered by available vaccines in Burkina Faso. These results will help guide our health policies towards better prevention of cervical cancer. The diversity of oncogenic genotypes is sparking a large-scale study in the West African sub-region, particularly in cases of cancer and the introduction of the nonavalent vaccine which includes HPV 52 found among the predominant genotypes in this study.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"125-136"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10014875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphism of MMP1 and MMP3 promoter regions and HR-HPV infection in women from Burkina Faso and Côte d'Ivoire. 布基纳法索和科特迪瓦妇女 MMP1 和 MMP3 启动子区域的多态性与 HR-HPV 感染。

Biomolecular Concepts Pub Date : 2020-05-17 DOI: 10.1515/bmc-2020-0010

Prosper Bado, Florencia Djigma Wendkuuni, Mahoukèdè Zohoncon Théodora, Dorcas Obiri-Yeboah, Alima Traoré Esther Mah, Karim Ouattara Abdoul, Clarisse Ouedraogo Teega-Wendé, Toyin Bello Shoukrat Ohuwa, Ayaovi Setor Marius, Angèle Traore Ina Marie, Apollinaire Horo, Privat Kouakou Kouame, Théophane Yonli Albert, Charlemagne Ouedraogo, Jacques Simpore

{"title":"Polymorphism of MMP1 and MMP3 promoter regions and HR-HPV infection in women from Burkina Faso and Côte d'Ivoire.","authors":"Prosper Bado, Florencia Djigma Wendkuuni, Mahoukèdè Zohoncon Théodora, Dorcas Obiri-Yeboah, Alima Traoré Esther Mah, Karim Ouattara Abdoul, Clarisse Ouedraogo Teega-Wendé, Toyin Bello Shoukrat Ohuwa, Ayaovi Setor Marius, Angèle Traore Ina Marie, Apollinaire Horo, Privat Kouakou Kouame, Théophane Yonli Albert, Charlemagne Ouedraogo, Jacques Simpore","doi":"10.1515/bmc-2020-0010","DOIUrl":"10.1515/bmc-2020-0010","url":null,"abstract":"The single nucleotide polymorphism (SNP) of the promoter region of MMP-1 (at 1607 bp) and MMP-3 (at 1171 bp) create Ets binding sites. Correlations between these SNPs and sensitivity to several biological processes such as metastasis and recurrence of cancer have been reported in several studies. In this case-control study, we looked for these SNPs in women infected with or not with high-risk human papillomaviruses (HR-HPV). The frequency, distribution and correlation of these SNPs with the presence or absence of HR-HPV infection were evaluated. Genotypes 1G1G, 1G2G and 2G2G for MMP1 and genotypes 5A5A, 5A6A, 6A6A for MMP3 were found in our study population. In general, we noted that the 1G (40.8%) and 2G (64.8%) alleles were more frequent in non-infected women and infected women, respectively, and more specifically this difference was significant in women from Côte d'Ivoire. These results, although yet to be reaffirmed with assays for quantifying the mRNA of these genes, suggest that the SNP of the MMP-1 promoter could promote infection with HR-HPV.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"116-124"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0