Biomolecular Concepts最新文献_第9页

Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid. 基于质谱技术对从卵泡液中分离出的总 IgG 和总蛋白质组 N-糖进行糖谱分析。

Biomolecular Concepts Pub Date : 2020-10-25 DOI: 10.1515/bmc-2020-0015

Marko Klobučar, Sanja Dević Pavlić, Iris Car, Neda Smiljan Severinski, Tamara Tramišak Milaković, Anđelka Radojčić Badovinac, Sandra Kraljević Pavelić

{"title":"Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid.","authors":"Marko Klobučar, Sanja Dević Pavlić, Iris Car, Neda Smiljan Severinski, Tamara Tramišak Milaković, Anđelka Radojčić Badovinac, Sandra Kraljević Pavelić","doi":"10.1515/bmc-2020-0015","DOIUrl":"10.1515/bmc-2020-0015","url":null,"abstract":"Couples with infertility issues have been assisted by in vitro fertilization reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro fertilization procedures, but other processes are also critical for fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing fertilization through either (1) assisted reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"153-171"},"PeriodicalIF":0.0,"publicationDate":"2020-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10390847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Conceptual Model for the Dual Role of FOF1-ATP Synthase in Cell Life and Cell Death. FOF1-ATP 合成酶在细胞生命和细胞死亡中双重作用的新概念模型。

Biomolecular Concepts Pub Date : 2020-08-22 DOI: 10.1515/bmc-2020-0014

Sunil Nath

{"title":"A Novel Conceptual Model for the Dual Role of FOF1-ATP Synthase in Cell Life and Cell Death.","authors":"Sunil Nath","doi":"10.1515/bmc-2020-0014","DOIUrl":"10.1515/bmc-2020-0014","url":null,"abstract":"The mitochondrial permeability transition (MPT) has been one of the longstanding enigmas in biology. Its cause is currently at the center of an extensive scientific debate, and several hypotheses on its molecular nature have been put forward. The present view holds that the transition arises from the opening of a high-conductance channel in the energy-transducing membrane, the permeability transition pore (PTP), also called the mitochondrial megachannel or the multiconductance channel (MMC). Here, the novel hypothesis is proposed that the aqueous access channels at the interface of the c-ring and the a-subunit of FO in the FOF1-ATP synthase are repurposed during induction of apoptosis and constitute the elusive PTP/ MMC. A unifying principle based on regulation by local potentials is advanced to rationalize the action of the myriad structurally and chemically diverse inducers and inhibitors of PTP/MMC. Experimental evidence in favor of the hypothesis and its differences from current models of PTP/MMC are summarized. The hypothesis explains in considerable detail how the binding of Ca2+ to a β-catalytic site (site 3) in the F1 portion of ATP synthase triggers the opening of the PTP/MMC. It is also shown to connect to longstanding proposals within Nath's torsional mechanism of energy transduction and ATP synthesis as to how the binding of MgADP to site 3 does not induce PTP/MMC, but instead catalyzes physiological ATP synthesis in cell life. In the author's knowledge, this is the first model that explains how Ca2+ transforms the FOF1-ATP synthase from an exquisite energy-conserving enzyme in cell life into an energy-dissipating structure that promotes cell death. This has major implications for basic as well as for clinical research, such as for the development of drugs that target the MPT, given the established role of PTP/MMC dysregulation in cancer, ischemia, cardiac hypertrophy, and various neurodegenerative diseases.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"143-152"},"PeriodicalIF":0.0,"publicationDate":"2020-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10071424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Endothelin-3 and Multiple Sclerosis. 对内皮素-3 和多发性硬化症的认识

Biomolecular Concepts Pub Date : 2020-06-25 DOI: 10.1515/bmc-2020-0012

Lucia Monti, Umberto Arrigucci, Alessandro Rossi

引用次数: 0

Erratum to "Polymorphism of MMP1 and MMP3 promoter regions and HR-HPV infection in women from Burkina Faso and Côte d'Ivoire". 对“MMP1和MMP3启动子区域多态性与布基纳法索和Côte科特迪瓦妇女HR-HPV感染”的勘误。

Biomolecular Concepts Pub Date : 2020-06-20 DOI: 10.1515/bmc-2020-0013

Prosper Bado, Florencia Wendkuuni Djigma, Théodora Mahoukèdè Zohoncon, Dorcas Obiri-Yeboah, Esther Mah Alima Traoré, Abdoul Karim Ouattara, Teega-Wendé Clarisse Ouedraogo, Shoukrat Ohuwa Toyin Bello, Marius Ayaovi Setor, Ina Marie Angèle Traore, Apollinaire Horo, Kouame Privat Kouakou, Théophane Albert Yonli, Charlemagne Ouedraogo, Jacques Simpore

引用次数: 1

Genotypic distribution of human oncogenic papillomaviruses in sexually active women in Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions. 布基纳法索性活跃妇女中人类致癌乳头瘤病毒的基因型分布：中部、中东部和上盆地地区。

Biomolecular Concepts Pub Date : 2020-05-17 DOI: 10.1515/bmc-2020-0011

Rogomenoma Alice Ouedraogo, Théodora Mahoukèdè Zohoncon, Ina Marie Angèle Traore, Abdoul Karim Ouattara, Sindimalgdé Patricia Guigma, Florencia Wendkuuni Djigma, Dorcas Obiri-Yeboah, Charlemagne Ouedraogo, Jacques Simpore

{"title":"Genotypic distribution of human oncogenic papillomaviruses in sexually active women in Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions.","authors":"Rogomenoma Alice Ouedraogo, Théodora Mahoukèdè Zohoncon, Ina Marie Angèle Traore, Abdoul Karim Ouattara, Sindimalgdé Patricia Guigma, Florencia Wendkuuni Djigma, Dorcas Obiri-Yeboah, Charlemagne Ouedraogo, Jacques Simpore","doi":"10.1515/bmc-2020-0011","DOIUrl":"10.1515/bmc-2020-0011","url":null,"abstract":"Objective this study was conducted to determine the distribution of high-risk human papillomavirus (HR-HPV) genotypes in women in the general population of three regions of Burkina Faso. Method This multicenter, descriptive cross-sectional study involved 1321 sexually active women in five cities in three regions of Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions. After collection of endocervical specimens, pre-cervical lesions were screened by visual inspection with acetic acid and lugol (VIA / VILI). HR-HPV genotypes were characterized by multiplex real-time PCR after extraction of viral DNA. Results The mean age of women was 31.98 ± 10.09 years. The HR-HPV infection in the three regions ranged from 26.16% to 43.26% with 35.42% as overall prevalence in women. The most common HR-HPV genotypes in descending order were: HPV 56, 52, 66, 59, 39, 51, 18, 35. The prevalence of bivalent vaccine genotypes (HPV16 / 18) was 7.83% against 63.78% of genotypes not covered by HPV vaccine; 36.32% (170/468) of women had multiple concomitant HR-HPV infections. Conclusion this study showed significant regional variation and high prevalence of HR-HPV infection in women. The predominant genotypes differ from those covered by available vaccines in Burkina Faso. These results will help guide our health policies towards better prevention of cervical cancer. The diversity of oncogenic genotypes is sparking a large-scale study in the West African sub-region, particularly in cases of cancer and the introduction of the nonavalent vaccine which includes HPV 52 found among the predominant genotypes in this study.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"125-136"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10014875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphism of MMP1 and MMP3 promoter regions and HR-HPV infection in women from Burkina Faso and Côte d'Ivoire. 布基纳法索和科特迪瓦妇女 MMP1 和 MMP3 启动子区域的多态性与 HR-HPV 感染。

Biomolecular Concepts Pub Date : 2020-05-17 DOI: 10.1515/bmc-2020-0010

Prosper Bado, Florencia Djigma Wendkuuni, Mahoukèdè Zohoncon Théodora, Dorcas Obiri-Yeboah, Alima Traoré Esther Mah, Karim Ouattara Abdoul, Clarisse Ouedraogo Teega-Wendé, Toyin Bello Shoukrat Ohuwa, Ayaovi Setor Marius, Angèle Traore Ina Marie, Apollinaire Horo, Privat Kouakou Kouame, Théophane Yonli Albert, Charlemagne Ouedraogo, Jacques Simpore

{"title":"Polymorphism of MMP1 and MMP3 promoter regions and HR-HPV infection in women from Burkina Faso and Côte d'Ivoire.","authors":"Prosper Bado, Florencia Djigma Wendkuuni, Mahoukèdè Zohoncon Théodora, Dorcas Obiri-Yeboah, Alima Traoré Esther Mah, Karim Ouattara Abdoul, Clarisse Ouedraogo Teega-Wendé, Toyin Bello Shoukrat Ohuwa, Ayaovi Setor Marius, Angèle Traore Ina Marie, Apollinaire Horo, Privat Kouakou Kouame, Théophane Yonli Albert, Charlemagne Ouedraogo, Jacques Simpore","doi":"10.1515/bmc-2020-0010","DOIUrl":"10.1515/bmc-2020-0010","url":null,"abstract":"The single nucleotide polymorphism (SNP) of the promoter region of MMP-1 (at 1607 bp) and MMP-3 (at 1171 bp) create Ets binding sites. Correlations between these SNPs and sensitivity to several biological processes such as metastasis and recurrence of cancer have been reported in several studies. In this case-control study, we looked for these SNPs in women infected with or not with high-risk human papillomaviruses (HR-HPV). The frequency, distribution and correlation of these SNPs with the presence or absence of HR-HPV infection were evaluated. Genotypes 1G1G, 1G2G and 2G2G for MMP1 and genotypes 5A5A, 5A6A, 6A6A for MMP3 were found in our study population. In general, we noted that the 1G (40.8%) and 2G (64.8%) alleles were more frequent in non-infected women and infected women, respectively, and more specifically this difference was significant in women from Côte d'Ivoire. These results, although yet to be reaffirmed with assays for quantifying the mRNA of these genes, suggest that the SNP of the MMP-1 promoter could promote infection with HR-HPV.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"116-124"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional reconstruction of individual helical nano-filament structures from atomic force microscopy topographs. 从原子力显微镜拓扑图三维重建单个螺旋纳米丝结构。

Biomolecular Concepts Pub Date : 2020-05-06 DOI: 10.1515/bmc-2020-0009

Liisa Lutter, Christopher J Serpell, Mick F Tuite, Louise C Serpell, Wei-Feng Xue

{"title":"Three-dimensional reconstruction of individual helical nano-filament structures from atomic force microscopy topographs.","authors":"Liisa Lutter, Christopher J Serpell, Mick F Tuite, Louise C Serpell, Wei-Feng Xue","doi":"10.1515/bmc-2020-0009","DOIUrl":"10.1515/bmc-2020-0009","url":null,"abstract":"Atomic force microscopy, AFM, is a powerful tool that can produce detailed topographical images of individual nano-structures with a high signal-to-noise ratio without the need for ensemble averaging. However, the application of AFM in structural biology has been hampered by the tip-sample convolution effect, which distorts images of nano-structures, particularly those that are of similar dimensions to the cantilever probe tips used in AFM. Here we show that the tip-sample convolution results in a feature-dependent and non-uniform distribution of image resolution on AFM topographs. We show how this effect can be utilised in structural studies of nano-sized upward convex objects such as spherical or filamentous molecular assemblies deposited on a flat surface, because it causes 'magnification' of such objects in AFM topographs. Subsequently, this enhancement effect is harnessed through contact-point based deconvolution of AFM topographs. Here, the application of this approach is demonstrated through the 3D reconstruction of the surface envelope of individual helical amyloid filaments without the need of cross-particle averaging using the contact-deconvoluted AFM topographs. Resolving the structural variations of individual macromolecular assemblies within inherently heterogeneous populations is paramount for mechanistic understanding of many biological phenomena such as amyloid toxicity and prion strains. The approach presented here will also facilitate the use of AFM for high-resolution structural studies and integrative structural biology analysis of single molecular assemblies.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"102-115"},"PeriodicalIF":0.0,"publicationDate":"2020-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical composition, antioxidant, anti-inflammatory and antiproliferative activities of the essential oil of Cymbopogon nardus, a plant used in traditional medicine. 传统医学中使用的一种植物--纳豆香茅（Cymbopogon nardus）精油的化学成分、抗氧化、抗炎和抗增殖活性。

Biomolecular Concepts Pub Date : 2020-04-15 DOI: 10.1515/bmc-2020-0007

Bagora Bayala, Ahmed Y Coulibaly, Florencia W Djigma, Bolni Marius Nagalo, Silvère Baron, Gilles Figueredo, Jean-Marc A Lobaccaro, Jacques Simpore

{"title":"Chemical composition, antioxidant, anti-inflammatory and antiproliferative activities of the essential oil of Cymbopogon nardus, a plant used in traditional medicine.","authors":"Bagora Bayala, Ahmed Y Coulibaly, Florencia W Djigma, Bolni Marius Nagalo, Silvère Baron, Gilles Figueredo, Jean-Marc A Lobaccaro, Jacques Simpore","doi":"10.1515/bmc-2020-0007","DOIUrl":"10.1515/bmc-2020-0007","url":null,"abstract":"Objectives Natural products commonly used in traditional medicine, such as essential oils (EOs), are attractive sources for the development of molecules with anti-proliferative activities for future treatment of human cancers, e.g., prostate and cervical cancer. In this study, the chemical composition of the EO from Cymbopogon nardus was characterized, as well as its antioxidativeproperties and anti-inflammatory and antiproliferative activities on LNCaP cells derived from prostate cancer. Methods The chemical composition of the EO was determined by GC/FID and GC/MS analyses. The antioxidative properties were assessed using DPPH radical scavenging assay and ABTS+• radical cation decolorization assay, and the anti-inflammatory capacity was determined by the inhibition of the lipoxygenase activity. Antiproliferative activity was evaluated by MTT assay. Results Collectively, our data show that the major constituents of C. nardus EO are citronellal (33.06 %), geraniol (28.40 %), nerol (10.94 %), elemol (5.25 %) and delta-elemene (4.09 %). C. nardus EO shows modest antioxidant and anti-inflammatory activity compared to the standard galic acid. C. nardus EO exhibits the best antiproliferative activity on the prostate cancer cell line LNCaP with an IC50 of 58.0 ± 7.9 μg/mL, acting through the induction of the cell cycle arrest. Conclusions This study has determined that C. nardus EO efficiently triggers cytotoxicity and pens a new field of investigation regarding the putative use of this EO in vivo.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"86-96"},"PeriodicalIF":0.0,"publicationDate":"2020-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of TNF-α-308G/A and IL-18 Polymorphisms with risk of HPV infection among sexually active women in Burkina Faso. TNF-α-308G/A 和 IL-18 多态性与布基纳法索性活跃妇女感染 HPV 风险的关系。

Biomolecular Concepts Pub Date : 2020-04-15 DOI: 10.1515/bmc-2020-0008

Ina M A Traore, Théodora M Zohoncon, Florencia W Djigma, T Rebeca Compaore, Yves Traore, Jacques Simpore

{"title":"Association of TNF-α-308G/A and IL-18 Polymorphisms with risk of HPV infection among sexually active women in Burkina Faso.","authors":"Ina M A Traore, Théodora M Zohoncon, Florencia W Djigma, T Rebeca Compaore, Yves Traore, Jacques Simpore","doi":"10.1515/bmc-2020-0008","DOIUrl":"10.1515/bmc-2020-0008","url":null,"abstract":"Human papillomavirus (HPV) infection is the most common sexually transmitted infection worldwide. Persistence infection can lead to the development of cervical cancer potentially due to some genetic factors such as polymorphisms in regulatory and coding regions of cytokine genes. The purpose of this study was to determine whether there is a relationship between TNF-308 G/A or IL18 polymorphisms and high-risk HPV infection among sexually active women from Burkina Faso. Ninety-one HPV infected and two hundred and nine HPV negative women (the latter used as healthy controls) were screened. TNFA-308 G/A and IL18-607 C/A polymorphisms were detected using the TaqMan allelic discrimination. HPV 52 (21.19%), HPV 39 (11.86%) and HPV 33 (11.02%) were the most common HPV genotypes. The TNFA-308A and IL18-607 C alleles were predominant in all women in the study. None of the TNFA and IL18 alleles were associated with HPV infection. The results suggest that there is no relationship between TNF-308 G/A or IL18-607C/A polymorphisms and HPV infection among women in the study.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"97-101"},"PeriodicalIF":0.0,"publicationDate":"2020-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10012327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Interferon-γ receptor (IFN-γR) expression in macrophages during Mycobacterium tuberculosis infection. 结核分枝杆菌感染期间巨噬细胞中干扰素-γ受体（IFN-γR）表达的调控。

Biomolecular Concepts Pub Date : 2020-04-06 DOI: 10.1515/bmc-2020-0006

Gunjan Kak, Brijendra K Tiwari, Yogendra Singh, Krishnamurthy Natarajan

{"title":"Regulation of Interferon-γ receptor (IFN-γR) expression in macrophages during Mycobacterium tuberculosis infection.","authors":"Gunjan Kak, Brijendra K Tiwari, Yogendra Singh, Krishnamurthy Natarajan","doi":"10.1515/bmc-2020-0006","DOIUrl":"10.1515/bmc-2020-0006","url":null,"abstract":"Interferon-gamma (IFN-γ) is a key cytokine that mediates immunity to tuberculosis (TB). Mycobacterium tuberculosis (M. tb) is known to downregulate the surface expression of IFN-γ receptor (IFN-γR) on macrophages and peripheral blood mononuclear cells (PBMCs) of patients with active TB disease. Many M. tb antigens also downmodulate IFN-γR levels in macrophages when compared with healthy controls. In the current study, we aimed at deciphering key factors involved in M. tb mediated downregulation of IFN-γR levels on macrophage surface. Our data showed that both M. tb H37Rv and M. bovis BCG infections mediate downmodulation of IFN-γR on human macrophages. This downmodulation is regulated at the level of TLR signaling pathway, second messengers such as calcium and cellular kinases i.e. PKC and ERK-MAPK, indicating that fine tuning of calcium response is critical to maintaining IFN-γR levels on macrophage surface. In addition, genes in the calcium and cysteine protease pathways which were previously identified by us to play a negative role during M. tb infection, also regulated IFN-γR expression. Thus, modulations in IFN-γR levels by utilizing host machinery may be a key immune suppressive strategy adopted by the TB pathogen to ensure its persistence and thwart host defense.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"11 1","pages":"76-85"},"PeriodicalIF":0.0,"publicationDate":"2020-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0