New Microbes and New Infections最新文献

{"title":"Prevalence and genotyping of human papillomavirus DNA in peripheral blood mononuclear cells from blood donors in Iran","authors":"Shahab Falahi ,&nbsp;Katayoun Arvaneh ,&nbsp;Hassan Nourmohammadi ,&nbsp;Amir Abdoli ,&nbsp;Arezoo Bozorgomid ,&nbsp;Azra Kenarkoohi","doi":"10.1016/j.nmni.2025.101593","DOIUrl":"10.1016/j.nmni.2025.101593","url":null,"abstract":"<div><h3>Introduction</h3><div>Human papillomavirus (HPV) is one of the most common sexually transmitted infections. In addition to sexual transmission, papillomavirus can also be transmitted through surfaces, autoinoculation and vertical transmission. Limited studies have also indicated the presence of HPV DNA in the blood of healthy individuals. The aim of this study was to investigate the prevalence of DNA in peripheral blood mononuclear cells (PBMCs) of blood donors of Ilam city-Iran.</div></div><div><h3>Material and methods</h3><div>We explored the prevalence of HPVs in 108 (58 males and 50 females) blood donors. DNA was extracted from the buffy coat, and genotyping was performed by sequencing after generic nested-PCR detection procedure using MY09/11 and GP5+/6+ primers targeting L1 region in HPV genome.</div></div><div><h3>Results</h3><div>HPV DNA was detected in 11 out of 108 samples (10.18 %). Genotype 16 was identified in 3 cases, followed by genotype 6 in 2 cases and genotypes 11, 31, 35, 51, 53, 59 each in one case. The positive cases included 4 men and 7 women, and their ages ranged from 25 to 37 years.</div></div><div><h3>Conclusion</h3><div>HPV DNA was detected in PBMCs of asymptomatic blood donors. These results support the concern about blood transfusion as a potential source of HPV transmission and need for future studies.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101593"},"PeriodicalIF":2.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance patterns of community-acquired urinary tract infections caused by Enterobacterales in Albania","authors":"Silvi Bozo ,&nbsp;Irida Ikonomi Hoxha ,&nbsp;Silva Tafaj ,&nbsp;Teuta Xhindi","doi":"10.1016/j.nmni.2025.101592","DOIUrl":"10.1016/j.nmni.2025.101592","url":null,"abstract":"<div><h3>Background</h3><div>The escalating rates of antibiotic resistance, especially through Extended-Spectrum Beta-Lactamase (ESBL) production within Enterobacterales represents a critical challenge in the effective management and treatment of urinary tract infections (UTIs). This study aims to examine the distribution of pathogens, assess the prevalence of ESBL production, and analyze the resistance patterns of Enterobacterales isolated from outpatient urocultures in Albania.</div></div><div><h3>Methods</h3><div>Midstream urine specimens from 406 patients were cultured on MacConkey agar and analyzed using the VITEK 2 automated system (bioMérieux, France). Samples with a bacterial count of ≥100,000 CFU/ml were considered positive for UTIs. Statistical analyses were conducted using SPSS software, version 27.0.</div></div><div><h3>Results</h3><div>Among the analyzed samples, 271 (66.7 %) tested positive for UTIs. Gram-negative bacteria, particularly Enterobacterales (88.5 %) were the most common while <em>E. coli</em> was the predominant microorganism (87.0 %). The demographic analysis revealed that females (74.5 %) and young adults (18–34 years) (54.0 %) were the most affected groups. High resistance rates were observed for tetracycline (55.0 %), piperacillin (43.0 %), and trimethoprim (38.5 %), while resistance to meropenem was very low (1.0 %). A total of 25 isolates (12.5 %) were positive for ESBL production. Among the <em>E. coli</em> isolates, 12.1 % (21/174) tested positive. Statistical analysis indicated a significant association between female gender and increased antibiotic resistance.</div></div><div><h3>Conclusions</h3><div>Our findings highlight the significant issue of antibiotic resistance in community-acquired UTIs in Albania, particularly involving Enterobacterales. Ongoing surveillance and tailored treatment strategies, alongside antibiotic stewardship programs, are crucial to address this growing public health concern.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101592"},"PeriodicalIF":2.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of Anopheles stephensi as a malaria vector in Sudan","authors":"Ibrahim Nagmeldin Hassan","doi":"10.1016/j.nmni.2025.101594","DOIUrl":"10.1016/j.nmni.2025.101594","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101594"},"PeriodicalIF":2.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measles outbreaks in the United States in 2025: Practice, policy, and the canary in the coalmine","authors":"Georgia-Leigh Hewitt ,&nbsp;Amir Obeid ,&nbsp;Philip R. Fischer","doi":"10.1016/j.nmni.2025.101591","DOIUrl":"10.1016/j.nmni.2025.101591","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101591"},"PeriodicalIF":2.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted next-generation sequencing enhances precision and rapid detection in healthcare-associated infection Surveillance: Unveiling multidrug-resistant colonization in ICUs","authors":"Tangchun Liu ,&nbsp;Shuyan Deng ,&nbsp;Wandi Liu,&nbsp;Jinzhao Zhang,&nbsp;Pengfei Wang ,&nbsp;Zhengfei Yang","doi":"10.1016/j.nmni.2025.101589","DOIUrl":"10.1016/j.nmni.2025.101589","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to evaluate the potential advantages of targeted next-generation sequencing (tNGS) over conventional bacterial culture methods for pathogen detection in hospital-associated infections (HAIs).</div></div><div><h3>Methods</h3><div>All EICU medical staff and all medical staff from the Physical Examination Centre completed a questionnaire. Nasopharyngeal specimens were collected from medical staff who met all of the inclusion criteria and none of the exclusion criteria. EICU medical staff provided 2 samples each, while Physical Examination Centre staff provided 1 sample each. For EICU medical staff, one of their two nasopharyngeal swabs was subjected to tNGS testing, and the other to bacterial culture testing. For the PEC staff, their nasopharyngeal swabs were subjected to tNGS testing. Additionally, six pairs of spectacles and six keyboards used by EICU medical staff were randomly selected, and the surfaces were swabbed with sterile swabs for tNGS testing.</div></div><div><h3>Results</h3><div>In 23 nasal swab samples from EICU group, tNGS detected 14 species of microorganism in 29 instances within 19 h. Bacterial culture detected 2 species of microorganism in 4 instances, 2 positive samples within 19 h and confirmed another 2 positive samples within 69 h. A total of 42 samples with 14 different microorganism species were collected from the nasopharyngeal swabs of 23 EICU members and 15 PEC members. Among them, 29 cases (69 %) of 14 different microorganisms were detected in EICU staff, with an average of 1.3 microorganism species detected per person, while 13 cases (28 %) of 6 different microorganisms were detected in PEC staff, with an average of 0.9 microorganism species detected per person. The most common colonizing bacteria included <em>Staphylococcus aureus</em>, <em>Acinetobacter baumannii</em>, and <em>Klebsiella</em> spp. Compared to bacterial culture, tNGS offers advantages in monitoring HAIs, including a broad range of detectable microorganisms, high sensitivity of results, and shorter reporting time for positive results. Bacteria colonizing the EICU carry more antibiotic resistance genes.</div></div><div><h3>Conclusions</h3><div>tNGS outperforms conventional culture in healthcare-associated infection surveillance, with higher sensitivity and accelerated pathogen identification. Simultaneously, tNGS revealed extensive colonization of multidrug-resistant (MDR) pathogens (e.g., <em>Acinetobacter baumannii</em>, MRSA) in EICU environments, highlighting its utility in monitoring complex antimicrobial resistance patterns.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101589"},"PeriodicalIF":2.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Quantitative microbial risk assessment of Legionella pneumophila in a drinking water distribution system: A case study” [New Microb New Infect 65 (2025) 101584]","authors":"Seyed Mohammad Ranjdoost ,&nbsp;Mina Owrang","doi":"10.1016/j.nmni.2025.101590","DOIUrl":"10.1016/j.nmni.2025.101590","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101590"},"PeriodicalIF":2.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antimicrobial resistance of gram-positive pathogens in Lebanon: The need for surveillance and stewardship","authors":"Yara Khachab ,&nbsp;Racha Khoumassi ,&nbsp;Elie Salem Sokhn","doi":"10.1016/j.nmni.2025.101588","DOIUrl":"10.1016/j.nmni.2025.101588","url":null,"abstract":"<div><h3>Background</h3><div>Resistance in Gram-positive organisms, including methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) and vancomycin-resistant <em>Enterococcus</em> (VRE), poses a significant healthcare challenge globally. However, data on these organisms in Lebanon remain limited. This retrospective study aimed to assess the prevalence and antimicrobial resistance patterns of <em>Staphylococcus aureus</em> (<em>S. aureus</em>), coagulase-negative <em>Staphylococci</em> (CoNS), and <em>Enterococcus</em> spp. in clinical infections at the Lebanese Hospital Geitaoui – UMC from 2017 to 2023.</div></div><div><h3>Methods</h3><div>A total of 2676 isolates were collected from urine, blood, respiratory specimens, and other infection sites. Bacterial identification was performed following WHO clinical bacteriology procedures, utilizing gram staining, catalase and coagulase tests, and biochemical assays. Antimicrobial susceptibility testing was conducted using the Kirby-Bauer disk diffusion method and minimum inhibitory concentration (MIC) analysis, interpreted according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Statistical analyses were performed using SPSS® version 24, with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>CoNS were the most prevalent (42.83 %), followed by <em>Enterococcus</em> spp. (28.81 %) and <em>S. aureus</em> (28.36 %). Blood cultures had the highest isolation rates (29.04 %), predominantly CoNS (76.45 %). <em>Enterococcus</em> spp. dominated urinary tract infections (85.01 %), while <em>S. aureus</em> was prevalent in wound/surgical site infections (59.23 %). Gender-specific trends showed CoNS and <em>S. aureus</em> more in males, while <em>Enterococcus</em> spp. infections were more common in females.</div></div><div><h3>Conclusion</h3><div>This study provides valuable insights into the prevalence and resistance patterns of Gram-positive pathogens in a Lebanese hospital setting. The findings highlight the need for continuous surveillance and stringent antibiotic stewardship to combat antimicrobial resistance effectively.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101588"},"PeriodicalIF":2.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arboviral infections and pregnancy: An overlooked public health challenge","authors":"Ibrahim Nagmeldin Hassan","doi":"10.1016/j.nmni.2025.101587","DOIUrl":"10.1016/j.nmni.2025.101587","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101587"},"PeriodicalIF":2.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota influences Plasmodium falciparum malaria susceptibility","authors":"Aly Kodio ,&nbsp;Drissa Coulibaly ,&nbsp;Safiatou Doumbo ,&nbsp;Salimata Konaté ,&nbsp;Abdoulaye Kassoum Koné ,&nbsp;Souleymane Dama ,&nbsp;Amadou Niangaly ,&nbsp;Mamadou Lamine Tall ,&nbsp;Ahmed Mohamed Konaté ,&nbsp;Coralie L'Ollivier ,&nbsp;A. Levasseur ,&nbsp;Fadi Bittar ,&nbsp;Abdoulaye Djimdé ,&nbsp;Ogobara K. Doumbo ,&nbsp;Didier Raoult ,&nbsp;Mahamadou Ali Thera ,&nbsp;Stéphane Ranque","doi":"10.1016/j.nmni.2025.101586","DOIUrl":"10.1016/j.nmni.2025.101586","url":null,"abstract":"<div><h3>Background</h3><div>The gut microbiota has recently been associated with malaria susceptibility/resistance in animal models and humans. This study aimed to assess its influence on malaria attack and <em>Plasmodium</em> parasitemia in children living in a malaria-endemic area of Mali.</div></div><div><h3>Methods</h3><div>Healthy children were enrolled in a 16-month cohort study in Bandiagara. Their gut bacteria and fungi community structures were characterized via 16S and ITS metabarcoding at enrolment. Clinicians monitored malaria attacks. Asymptomatic <em>Plasmodium</em> carriage was assessed by real-time polymerase chain reaction.</div></div><div><h3>Results</h3><div>Of the 300 children, 107 (36 %) had at least one malaria attack, and 82 (27 %) had at least one episode of asymptomatic <em>Plasmodium</em> parasitemia. The gut bacterial community structure, but not the fungal community, was associated with susceptibility/resistance to both malaria attacks and asymptomatic <em>P. falciparum</em> parasitemia. Higher gut bacteria richness was independently associated with susceptibility to both asymptomatic parasitemia episodes and malaria attacks. 17 bacteria, and 7 fungi were associated with susceptibility to malaria attacks, and 8 bacteria, and 3 fungi were associated with resistance. 15 bacteria and 13 fungi were associated with susceptibility to asymptomatic <em>Plasmodium</em> parasitemia episodes, and 19 bacteria and 3 fungi were associated with resistance.</div></div><div><h3>Conclusion</h3><div>Further studies are needed to confirm these findings, which point the way to strategies aimed at reducing the risk of malaria by modulating gut microbiota components in at-risk populations.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101586"},"PeriodicalIF":2.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mogibacterium bacteraemia in an elderly woman with severe periodontal disease","authors":"Victoria Jordan ,&nbsp;Syeda Naqvi","doi":"10.1016/j.nmni.2025.101585","DOIUrl":"10.1016/j.nmni.2025.101585","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101585"},"PeriodicalIF":2.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}