MicroRNA (Shariqah, United Arab Emirates)最新文献_第8页

Plant MicroRNA Potential in Targeting Sars-CoV-2 Genome Offering Efficient Antiviral MiRNA-Based Therapies. 植物MicroRNA靶向Sars-CoV-2基因组的潜力提供有效的基于MicroRNA的抗病毒治疗

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220818150634

Behzad Hajieghrari, Sara Rahmanian-Koshkaki

{"title":"Plant MicroRNA Potential in Targeting Sars-CoV-2 Genome Offering Efficient Antiviral MiRNA-Based Therapies.","authors":"Behzad Hajieghrari, Sara Rahmanian-Koshkaki","doi":"10.2174/2211536611666220818150634","DOIUrl":"https://doi.org/10.2174/2211536611666220818150634","url":null,"abstract":"Background: In 2019, severe acute respiratory coronavirus II (or SARS-COV-2) emerged in Wuhan, China, rapidly becoming a global pandemic. Coronavirus genus (Coronaviridae) has the largest single-stranded positive-sense RNA genome (~30 kb) among the human infected single-stranded RNA viruses.Objectives: For the study of active therapeutic plant-derived miRNA(s), it may be possible to uptake the miRNAs and their biological role in the host cell. In this study, we bioinformatically searched plant miRNAs that can potentially interact with the Sars-CoV-2 genome within the 3'- UTR region and have prompt antiviral activity.Materials and methods: We searched the plant miRNAs that target the 3'-UTR flanking region of the Sars-CoV-2 genome by employing the RNAHybrid, RNA22, and STarMir miRNA/target prediction tools.Results: The RNAHybrid algorithm found 63 plant miRNAs having hybridization energy with less or equal to -25 kcal.mol-1. Besides, RNA22 and STarMir tools identified eight interactions between the plant miRNAs and the targeted RNA sequence. pvu-miR159a. 2 and sbi-miR5387b were predicted as the most effectively interacting miRNAs in targeting the 3'-UTR sequence, not only by the RNA22 tool but also by the STarMir tool at the same position. However, the GC content of the pvumiR159a. 2 is 55% instead of sbi-miR5387b, which is a GC enriched sequence (71.43%) that may activate TLR receptors.Conclusion: In our opinion, they are potent plant-derived miRNA candidates that have a great chance of targeting the Sars-CoV-2 genome in the 3'-UTR region in vitro. Therefore, we propose pvu-miR159a.2 for studying antiviral miRNA-based therapies without any essential side effects in vivo.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 3","pages":"245-262"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10511410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs as Biomarkers for Birth Defects. 作为出生缺陷生物标志物的微RNA。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220215123423

Ratnam S Seelan, M Michele Pisano, Robert M Greene

引用次数: 0

Identification of Candidate Immune System MicroRNAs Differentially Found in Colostrum and Milk Exosomes. 初乳和乳外泌体中差异的候选免疫系统microrna鉴定。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220630102316

Poonam Verma, Niharika Mohanty, Babita Pruseth, Sonali Sahoo, Amit Katiyar, Harpreet Singh, Saubhagya Kumar Jena, Rashmi Ranjan Das, Tapas Kumar Som, Sanjeeb Kumar Sahoo, Pranati Nanda, Amit Ghosh

{"title":"Identification of Candidate Immune System MicroRNAs Differentially Found in Colostrum and Milk Exosomes.","authors":"Poonam Verma, Niharika Mohanty, Babita Pruseth, Sonali Sahoo, Amit Katiyar, Harpreet Singh, Saubhagya Kumar Jena, Rashmi Ranjan Das, Tapas Kumar Som, Sanjeeb Kumar Sahoo, Pranati Nanda, Amit Ghosh","doi":"10.2174/2211536611666220630102316","DOIUrl":"https://doi.org/10.2174/2211536611666220630102316","url":null,"abstract":"Background: The fetus grows in a sterile womb environment. After birth, the newborn immune system has two immediate hurdles to clear. First immediate suppression of the womb compatible immune system and turn on the immune system of the newborn that can counter the antigenic world. The underlying mechanism of immune fluctuation by milk microRNAs (miRNAs) can be crucial for the treatment of critical or premature newborn.Methods: We collected fourteen samples of each colostrum and mature milk from lactating mothers, four samples of each were used for microarray analysis, and the other ten were used for miRNA expression profiling by real-time PCR.Results: From the microarray, 154 differentially expressed miRNAs were identified, whereas 49 miRNAs were revealed as immune-related miRNAs based on a literature study. Among the 49 miRNAs, 33 were already shown as strongly validated immune-related miRNAs (validated by qPCR, Western Blot, and Luciferase assay) and were considered for further analysis. Twenty-two miRNA expressions were analysed by real-time PCR as their Ct values were within considerable limits. Twelve numbers of miRNAs were significantly downregulated in mature milk compared to colostrum, which were again subjected to bioinformatics analysis to predict the biological mechanisms behind the differentially expressed miRNAs.Conclusion: This study shed light on the human milk exosome miRNA expression dynamics during lactation and their possible role in the gradual skewing of the newborns' immune system. The information is crucial for the development and onset of sepsis in premature newborns in the NICU.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 3","pages":"216-226"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10801713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Prognostic Value and Biological Role of miR-126 in Breast Cancer. miR-126在乳腺癌中的预后价值及生物学作用

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/1876402914666220428123203

Saiedeh Razi Soofiyani, Kamran Hosseini, Tahereh Ebrahimi, Haleh Forouhandeh, Mohammadreza Sadeghi, Sohrab Minaei Beirami, Tohid Ghasemnejad, Vahideh Tarhriz, Soheila Montazersaheb

{"title":"Prognostic Value and Biological Role of miR-126 in Breast Cancer.","authors":"Saiedeh Razi Soofiyani, Kamran Hosseini, Tahereh Ebrahimi, Haleh Forouhandeh, Mohammadreza Sadeghi, Sohrab Minaei Beirami, Tohid Ghasemnejad, Vahideh Tarhriz, Soheila Montazersaheb","doi":"10.2174/1876402914666220428123203","DOIUrl":"https://doi.org/10.2174/1876402914666220428123203","url":null,"abstract":"In eukaryotic organisms such as humans, some noncoding single-stranded RNAs (ncRNAs) contribute to regulating the expression of some genes before and after the transcription process, which in turn controls a number of vital physiological processes, including cell proliferation, differentiation, invasion, angiogenesis, and embryonic development. miR-126 is one of these miRNAs expressed exclusively in endothelial cells such as capillaries and vessels involved in controlling angiogenesis. In recent years, the link between miRs such as miR-126 and the pathology of breast cancer has attracted the attention of many researchers. Numerous studies have shown that miR-126 may be able to suppress tumor tissue metastasis or to increase tumor metastasis through complex molecular mechanisms. There is ample clinical evidence that miR-126 can be used as a biomarker to predict and diagnose breast cancer due to the increased or decreased expression of certain genes in breast cancer tissue. In this review, we discuss the association between the growth and metastasis (tumorigenesis) of breast cancer and miR-126, as well as the relationship between current research advances in the prognosis, diagnosis, and treatment of breast cancer and miR-126.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 2","pages":"95-103"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10452210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Diagnostic Value of Potential MicroRNAs in CRC: A Meta-Analysis. 潜在microrna在CRC中的诊断价值:一项荟萃分析。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220523103316

Sofia Fathi, Fadila Guessous, Mehdi Karkouri

{"title":"Diagnostic Value of Potential MicroRNAs in CRC: A Meta-Analysis.","authors":"Sofia Fathi, Fadila Guessous, Mehdi Karkouri","doi":"10.2174/2211536611666220523103316","DOIUrl":"https://doi.org/10.2174/2211536611666220523103316","url":null,"abstract":"Background: MicroRNAs (miRNAs) are small noncoding RNA molecules involved in the post-transcriptional regulation of genes. Deregulated expression of miRNAs is involved in different pathogenic mechanisms, particularly colorectal cancer (CRC) carcinogenesis. Due to their stability and accessibility, circulating miRNAs represent a new family of biomarkers with great potential. Therefore, certain miRNAs can be used as diagnostic biomarkers in CRC.Objective: This systematic analysis aimed to explore the individual efficacy of the most investigated blood-based miRNAs for CRC diagnosis, namely miR-21, miR-29a and miR-92a.Methods: Articles were retrieved from databases such as PubMed and Google Scholar, and studies designed to evaluate the diagnostic value of microRNAs in CRC were then selected. We subsequently explored the diagnostic accuracy of each miRNA using parameters such as (SE, SPE, PLR, NLR). The meta-analysis was performed using the Review Manager (Revman) 5.4 software and the Meta Disc software.Results: Our results suggested that serum miR-21 levels showed great potential as a diagnostic molecular marker. The overall pooled results for sensitivity, specificity, area under the curve (AUC), PLR, and NLR were 78%, 91%, 0.9519, 8.12 and 0.17, respectively.Conclusion: miRNAs have become increasingly important in the diagnosis of CRC. Based on these findings, circulating miR-21 levels may have a potential value for early detection and might be used as a novel diagnostic biomarker for CRC.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 3","pages":"190-205"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10453923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

MicroRNA-16 Represses TGF-β1-induced Epithelial-to-Mesenchymal Transition in Human Lung Adenocarcinoma Cell Line. MicroRNA-16抑制TGF-β1诱导的人肺腺癌细胞上皮向间质转化

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220826124058

Subbiah Rajasekaran, Sehal Mishra, Deepa Gandhi

{"title":"MicroRNA-16 Represses TGF-β1-induced Epithelial-to-Mesenchymal Transition in Human Lung Adenocarcinoma Cell Line.","authors":"Subbiah Rajasekaran, Sehal Mishra, Deepa Gandhi","doi":"10.2174/2211536611666220826124058","DOIUrl":"https://doi.org/10.2174/2211536611666220826124058","url":null,"abstract":"Background: The transforming growth factor-beta1 (TGF-β1)-induced epithelial-tomesenchymal transition (EMT) has a crucial effect on the progression and metastasis of lung cancer cells.Objective: The purpose of this study was to investigate whether microRNA (miR)-16 can suppress TGF-β1-induced EMT and proliferation in human lung adenocarcinoma cell line (A549).Methods: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-16. The hallmarks of EMT were assessed by RT-qPCR, Western blotting, and cell proliferation assay. A bioinformatics tool was used to identify the putative target of miR-16. The activation of TGF-β1/Smad3 signaling was analysed using Western blotting.Results: Our results showed that miR-16 expression was significantly down-regulated by TGF-β1 in A549 cells. Moreover, agomir of miR-16 suppressed TGF-β1-induced EMT and cell proliferation. Computational algorithms predicted that the 3'-untranslated regions (3'-UTRs) of Smad3 are direct targets of miR-16. In addition, miR-16 mimic was found to inhibit the TGF-β1-induced activation of the TGF-β1/Smad3 pathway, suggesting that miR-16 may function partly through regulating Smad3.Conclusion: Our results demonstrated that overexpression of miR-16 suppressed the expression and activation of Smad3, and ultimately inhibited TGF-β1-induced EMT and proliferation in A549 cells. The present findings support further investigation of the anti-cancer effect of miR-16 in animal models of lung cancer to validate the therapeutic potential.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 3","pages":"206-215"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10454411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

P68 RNA Helicase (DDX5) Required for the Formation of Various Specific and Mature miRNA Active RISC Complexes. 形成各种特异性和成熟的miRNA活性RISC复合物所需的P68 RNA螺旋酶（DDX5）。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220218121640

Mariette Kokolo, Montse Bach-Elias

{"title":"P68 RNA Helicase (DDX5) Required for the Formation of Various Specific and Mature miRNA Active RISC Complexes.","authors":"Mariette Kokolo, Montse Bach-Elias","doi":"10.2174/2211536611666220218121640","DOIUrl":"10.2174/2211536611666220218121640","url":null,"abstract":"Introduction: DEAD-box RNA helicases catalyze the ATP-dependent unwinding of doublestranded RNA. In addition, they are required for protein displacement and remodelling of RNA or RNA/protein complexes. P68 RNA helicase regulates the alternative splicing of the important protooncogene H-Ras, and numerous studies have shown that p68 RNA helicase is probably involved in miRNA biogenesis, mainly through Drosha and RISC/DICER complexes.Objective: This study aimed to determine how p68 RNA helicase affects the activity of selected mature miRNAs, including miR-342, miR-330, miR-138 and miR-206, miR-126, and miR-335, and let-7a, which are known to be related to cancer processes.Methods: The miRNA levels were analyzed in stable HeLa cells containing p68 RNA helicase RNAi induced by doxycycline (DOX). Relevant results were repeated using transient transfection with pSuper/ pSuper-p68 RNA helicase RNAi to avoid DOX interference.Results: Herein, we reported that p68 RNA helicase downregulation increases the accumulation of the mature miRNAs, such as miR-126, let-7a, miR-206, and miR-138. Interestingly, the accumulation of these mature miRNAs does not downregulate their known protein targets, thus suggesting that p68 RNA helicase is required for mature miRNA-active RISC complex activity.Conclusion: Furthermore, we demonstrated that this requirement is conserved, as drosophila p68 RNA helicase can complete the p68 RNA helicase depleted activity in human cells. Dicer and Drosha proteins are not affected by the downregulation of p68 RNA helicase despite the fact that Dicer is also localized in the nucleus when p68 RNA helicase activity is reduced.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 1","pages":"36-44"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39635332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Meet the Editorial Board Member 会见编辑委员会成员

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2021-12-01 DOI: 10.2174/221153661004211231212125

K. Dua

引用次数: 0

Meet the Associate Editor 与副主编见面

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2021-03-01 DOI: 10.2174/221153661001210820092316

W. Tam

引用次数: 0

Preface 前言

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2021-03-01 DOI: 10.2174/221153661001210820091622

Alberto Izzotti

引用次数: 0