MicroRNA (Shariqah, United Arab Emirates)最新文献_第7页

Identification of Prognostic Biomarkers in Papillary Thyroid Cancer and Developing Non-Invasive Diagnostic Models Through Integrated Bioinformatics Analysis. 通过综合生物信息学分析鉴定甲状腺乳头状癌预后生物标志物并建立无创诊断模型。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220124115445

Afsaneh Arefi Oskouie, Mohammad Saeed Ahmadi, Amir Taherkhani

{"title":"Identification of Prognostic Biomarkers in Papillary Thyroid Cancer and Developing Non-Invasive Diagnostic Models Through Integrated Bioinformatics Analysis.","authors":"Afsaneh Arefi Oskouie, Mohammad Saeed Ahmadi, Amir Taherkhani","doi":"10.2174/2211536611666220124115445","DOIUrl":"https://doi.org/10.2174/2211536611666220124115445","url":null,"abstract":"BACKGROUND\u0000Papillary thyroid cancer (PTC) is the most frequent subtype of thyroid carcinoma, mainly detected in patients with benign thyroid nodules (BTN). Due to the invasiveness of accurate diagnostic tests, there is a need to discover applicable biomarkers for PTC. So, in this study, we aimed to identify the genes associated with prognosis in PTC. Besides, we performed a machine learning tool to develop a non-invasive diagnostic approach for PTC.\u0000\u0000\u0000METHODS\u0000For the study's purposes, the miRNA dataset GSE130512 was downloaded from the GEO database and then analyzed to identify the common differentially expressed miRNAs in patients with non-metastatic PTC (nm-PTC)/metastatic PTC (m-PTC) compared with BTNs. The SVM was also applied to differentiate patients with PTC from those patients with BTN using the common DEMs. A protein-protein interaction network was also constructed based on the targets of the common DEMs. Next, functional analysis was performed, the hub genes were determined, and survival analysis was then executed.\u0000\u0000\u0000RESULTS\u0000A total of three common miRNAs were found to be differentially expressed among patients with nm-PTC/m-PTC compared with BTNs. In addition, it was established that the autophagosome maturation, ciliary basal body-plasma membrane docking, antigen processing as ubiquitination & proteasome degradation, and class I MHC mediated antigen processing & presentation are associated with the pathogenesis of PTC. Furthermore, it was illustrated that RPS6KB1, CCNT1, SP1, and CHD4 might serve as new potential biomarkers for PTC prognosis.\u0000\u0000\u0000CONCLUSIONS\u0000RPS6KB1, CCNT1, SP1, and CHD4 may be considered as new potential biomarkers used for prognostic aims in PTC. However, performing validation tests is inevitable in the future.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 1","pages":"73-87"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39850738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Screening and in Silico Functional Analysis of MiRNAs Associated with Acute Myeloid Leukemia Relapse. 与急性髓性白血病复发相关的mirna的筛选和计算机功能分析。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220511160502

Ali Amini Fard, Hamzeh Rahimi, Zinat Shams, Pegah Ghoraeian

{"title":"Screening and in Silico Functional Analysis of MiRNAs Associated with Acute Myeloid Leukemia Relapse.","authors":"Ali Amini Fard, Hamzeh Rahimi, Zinat Shams, Pegah Ghoraeian","doi":"10.2174/2211536611666220511160502","DOIUrl":"https://doi.org/10.2174/2211536611666220511160502","url":null,"abstract":"Background: Hematologic malignancies are among fatal diseases with different subtypes. Acute myeloid leukemia (AML) is a subtype showing a high invasion rate to different tissues.Objective: AML patients, even after treatment, show an increased rate of recurrence, and this relapsed profile of AML has turned this malignancy into big challenges in the medical scope.Methods: In the current study, we aimed to investigate hub-genes and potential signaling pathways in AML recurrence. Two expression profiles of genes and non-coding RNAs were extracted from the Gene Expression Omnibus (GEO) database. Target genes of identified miRNAs were predicted through bioinformatics tools. GO and KEGG pathway enrichment analyses were conducted to discover common target genes and differentially expressed genes. Protein-protein interaction (PPI) network was constructed and visualized through the STRING online database and Cytoscape software, respectively. Hub-genes of constructed PPI were found through the CytoHubba plugin of Cytoscape software.Results: As a result, 109 differentially expressed genes and 45 differentially expressed miRNAs were found, and the top enriched pathways were immune response, xhemokine activity, immune System, and plasma membrane. The hub-genes were TNF, IL6, TLR4, VEGFA, PTPRC, TLR7, TLR1, CD44, CASP1, and CD68.Conclusion: The present investigation based on the in silico analysis and microarray GEO databases may provide a novel understanding of the mechanisms related to AML relapse.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 3","pages":"227-244"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10445173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Perspectives on Epigenetic Markers in Adaptation to Physical Exercise. 体育运动适应的表观遗传标记研究进展

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220318140844

Robert Solsona, Fabio Borrani, Henri Bernardi, Anthony M J Sanchez

引用次数: 0

Novel Biomarkers of microRNAs in Gastric Cancer: An Overview from Diagnosis to Treatment. 胃癌中微小rna的新生物标志物:从诊断到治疗的综述。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220322160242

Ebrahim Mirzajani, Sogand Vahidi, Seyedeh Elham Norollahi, Ali Akbar Samadani

{"title":"Novel Biomarkers of microRNAs in Gastric Cancer: An Overview from Diagnosis to Treatment.","authors":"Ebrahim Mirzajani, Sogand Vahidi, Seyedeh Elham Norollahi, Ali Akbar Samadani","doi":"10.2174/2211536611666220322160242","DOIUrl":"https://doi.org/10.2174/2211536611666220322160242","url":null,"abstract":"Gastric cancer (GC) is the fourth most frequent disease in the world and the second cause of cancer-related death. In this way, over 80% of diagnoses are made in the middle to advanced degrees of the disease, underscoring the requirement for innovative biomarkers that can be identified quickly. Meaningly, biomarkers that can complement endoscopic diagnosis and be used to detect patients with a high risk of GC are desperately needed. These biomarkers will allow for the accurate prediction of therapy response and prognosis in GC patients, as well as the development of an optimal treatment strategy for each individual. Conspicuously, microRNAs (miRNAs) and small noncoding RNA regulate the expression of target mRNA, thereby modifying critical biological mechanisms. According to the data, abnormally miRNAs expression in GC is linked to tumor growth, carcinogenesis, aggression, and distant metastasis. Importantly, miRNA expression patterns and nextgeneration sequencing (NGS) can also be applied to analyze different kinds of tissues and cancers. Given the high death rates and poor prognosis of GC, and the absence of a clinical diagnostic factor that is adequately sensitive to GC, research on novel sensitive and specific markers for GC diagnosis is critical. In this review, we examine the latest research findings that suggest the feasibility and clinical utility of miRNAs in GC.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":"12-24"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40315650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Downregulation of miR-125a-5p Leads to STAT3 Increased Expression in Breast Cancer Patients. miR-125a-5p下调导致STAT3在乳腺癌患者中的表达升高。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220907125812

Negar Shafagh Shishavan, Soheila Talesh Sasani, Zivar Salehi, Masoumeh Rezaei Azhang

{"title":"Downregulation of miR-125a-5p Leads to STAT3 Increased Expression in Breast Cancer Patients.","authors":"Negar Shafagh Shishavan, Soheila Talesh Sasani, Zivar Salehi, Masoumeh Rezaei Azhang","doi":"10.2174/2211536611666220907125812","DOIUrl":"https://doi.org/10.2174/2211536611666220907125812","url":null,"abstract":"Background: Breast cancer (BC) is one of the main causes of cancer-related death in women worldwide. It is necessary to find methods for prognosis and early detection of BC. MicroRNAs inhibit the expression of special target genes at the post-transcriptional stage and have a fundamental role in various cancers. They function as oncogenes or tumor suppressors. MiR-125a- 5p acts as a tumor suppressor in some cancers through a signal transducer and activator of transcription 3 (STAT3) suppression. STAT3 is activated in response to cytokines and growth factors, affecting the transcription of target genes.Objective: We examined the association between miR-125a-5p and STAT3 expression levels in breast cancer patients for the first time through a case-control study on an Iranian population.Methods: Total RNAs were extracted from breast cancer and healthy tissues using TRIzol Reagent. Complementary DNA synthesis was performed, and Real-time PCR was done using miR-125a and STAT3-specific primers. GAPDH and U48 genes were used as internal controls. Statistical analysis of the results was conducted by SPSS v.19.0 software.Results: We obtained a significant association between miR-125a-5p down-regulation and breast cancer disease (0.4333 in patients vs. 1.656 in controls, p-value = 0.009). STAT3 expression was significantly up-regulated in BC samples relative to healthy subjects (1.324 vs. 0.6557, respectively) and p-value <0.0001.Conclusion: We investigated that decreased miR-125a-5p expression levels were significantly associated with increased STAT3 expression in BC tissues. Therefore, the expression changes of miR- 125a-5p can be an important potential biomarker for early diagnosis of breast cancer. Also, the miRNA molecule may have serious therapeutic potential.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 3","pages":"263-270"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10452726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role, Significance, and Association of MicroRNA-10a/b in Physiology of Cancer. MicroRNA-10a/b在肿瘤生理中的作用、意义和关联

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220523104408

Khaled M Elgeshy, Abdel Hady A Abdel Wahab

引用次数: 0

The Interaction Network of MicroRNAs with Cytokines and Signaling Pathways in Allergic Asthma. 过敏性哮喘中microrna与细胞因子及信号通路的相互作用网络。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220428134324

Ali Farmanzadeh, Durdi Qujeq, Tooba Yousefi

引用次数: 2

The Core Human MicroRNAs Regulated by Toxoplasma gondii. 刚地弓形虫调控的核心人类microrna。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220428130250

Neelam Antil, Mohammad Arefian, Mrudula Kinarulla Kandiyil, Kriti Awasthi, Thottethodi Subrahmanya Keshava Prasad, Rajesh Raju

{"title":"The Core Human MicroRNAs Regulated by Toxoplasma gondii.","authors":"Neelam Antil, Mohammad Arefian, Mrudula Kinarulla Kandiyil, Kriti Awasthi, Thottethodi Subrahmanya Keshava Prasad, Rajesh Raju","doi":"10.2174/2211536611666220428130250","DOIUrl":"https://doi.org/10.2174/2211536611666220428130250","url":null,"abstract":"Background: Toxoplasma gondii (T. gondii) is an intracellular zoonotic protozoan parasite known to effectively modulate the host system for its survival. A large number of microRNAs (miRNAs) regulated by different strains of T. gondii in diverse types of host cells/tissues/organs have been reported across multiple studies.Objective: We aimed to decipher the complexity of T. gondii regulated spectrum of miRNAs to derive a set of core miRNAs central to different strains of T. gondii infection in diverse human cell lines.Methods: We first assembled miRNAs hat are regulated by T. gondii altered across the various assortment of infections and time points of T. gondii infection in multiple cell types. For these assembled datasets, we employed specific criteria to filter the core miRNAs regulated by T. gondii. Subsequently, accounting for the spectrum of miRNA-mRNA target combinations, we applied a novel confidence criterion to extract their core experimentally-validated mRNA targets in human cell systems.Results: This analysis resulted in the extraction of 74 core differentially regulated miRNAs and their 319 high-confidence mRNA targets. Based on these core miRNA-mRNA pairs, we derived the central biological processes perturbed by T. gondii in diverse human cell systems. Further, our analysis also resulted in the identification of novel autocrine/paracrine signalling factors that could be associated with host response modulated by T. gondii.Conclusion: The current analysis derived a set of core miRNAs, their targets, and associated biological processes fine-tuned by T. gondii for its survival within the invaded cells.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 2","pages":"163-174"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10458423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The Use of Machine Learning in MicroRNA Diagnostics: Current Perspectives. 机器学习在MicroRNA诊断中的应用:当前观点。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220818145553

Chrysanthos D Christou, Angelos C Mitsas, Ioannis Vlachavas, Georgios Tsoulfas

{"title":"The Use of Machine Learning in MicroRNA Diagnostics: Current Perspectives.","authors":"Chrysanthos D Christou, Angelos C Mitsas, Ioannis Vlachavas, Georgios Tsoulfas","doi":"10.2174/2211536611666220818145553","DOIUrl":"https://doi.org/10.2174/2211536611666220818145553","url":null,"abstract":"MicroRNAs constitute small non-coding RNAs that play a pivotal role in regulating the translation and degradation of mRNA and have been associated with many diseases. Artificial Intelligence (AI) is an evolving cluster of interrelated fields, with machine learning (ML) standing out as one of the most prominent AI fields, with a plethora of applications in almost every aspect of human life. ML could be defined as computer algorithms that learn from past data to predict future data. This review comprehensively reviews the current applications of microRNA-based ML models in healthcare. The majority of the identified studies investigated the role of microRNA-based ML models in the management of cancer and specifically gastric cancer (maximum diagnostic accuracy (Accmax): 94%), pancreatic cancer (Accmax: 93%), colorectal cancer (Accmax: 100%), breast cancer (Accmax: 97%), ovarian cancer, neck squamous cell carcinoma, liver cancer, lung cancer (Accmax: 100%), and melanoma. Except for cancer, microRNA-based ML models have been applied for a plethora of other diseases, including ulcerative colitis (Accmax: 92.8%), endometriosis, gestational diabetes mellitus (Accmax: 86%), hearing loss, ischemic stroke, coronary heart disease (Accmax: 96%), tuberculosis, pulmonary arterial hypertension (Accmax: 83%), dementia (Accmax: 82.9%), major cardiovascular events in end-stage renal disease patients, and alcohol dependence (Accmax: 79.1%). Our findings suggest that the development of microRNA-based ML models could be used to enhance the diagnostic accuracy of a plethora of diseases while at the same time substituting or minimizing the use of more invasive diagnostic means (such as endoscopy). Even not as fast as anticipated, AI will eventually infiltrate the entire healthcare industry. AI is the key to a clinical practice where medicine's inherent complexity is embraced. Therefore, AI will become a reality that physicians should conform with to avoid becoming obsolete.","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":"11 3","pages":"175-184"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10511408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Tiny Regulators in Viral Infection: Carving SARS-CoV-2 by miRNAs. 病毒感染中的微小调节因子:通过mirna雕刻SARS-CoV-2。

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2022-01-01 DOI: 10.2174/2211536611666220816124650

Natalia Martínez-Acuña, Sonia Amelia Lozano-Sepúlveda, María Del Carmen Martínez-Guzmán, Ana María Rivas-Estilla

引用次数: 0