OpenNano最新文献

{"title":"Enhanced immunomodulator activity of Carthamus tinctorius (L.) extracts, a traditional medicine using nanostructured lipid carrier approach","authors":"Rachmat Mauludin ,&nbsp;Insan Nurul Padjar ,&nbsp;Neng Fisheri Kurniati ,&nbsp;Ratna Annisa Utami","doi":"10.1016/j.onano.2025.100244","DOIUrl":"10.1016/j.onano.2025.100244","url":null,"abstract":"<div><div>Considering the potential of <em>Carthamus tinctorius (</em>L.<em>)</em> (CT) for therapeutic purposes mainly as immunomodulator as well as the ability of nanostructured lipid carriers (NLC) to protect phytochemicals from degradation and improving their solubility, the objective of this study was design, optimization, and characterization CT-loaded NLC (CT<img>NLC) system as an immunomodulator. The NLCs were prepared using hot homogenization techniques, which were later subjected to ultrasonication according to the Box Behnken design. The NLCs were characterized in terms of polydispersity index (PDI), particle size (PS), zeta potential (ZP), entrapment efficiency (%EE), and surface morphologies. The immunomodulatory activity was carried out by measuring phagocytic activity using carbon clearance method, organ index, antibody titres using indirect hemagglutination test and level of interferon-gamma (IFN-γ). The CT extracts (300 mg/kg), CT-NLC (100 mg/kg and 300 mg/kg) were administered orally. Levamisole (3 mg/kg) and Methylprednisolone (16 mg/kg) was used as standard drug. The selected CT<img>NLC displayed a spherical particle with an average of ∼199.6 nm, polydispersity index of ∼0.305 and zeta potential of ∼-27.4 mV. %EE ∼74.04 % was attained. TEM images exhibited nanoparticles to be non-aggregated and in spherical shape. CTNLC treatment significantly increased the phagocytic index, antibody titres and level of IFN-γ but did not influence organ index compared to free CT. <em>Carthamus tinctorius (</em>L.<em>)</em> was successfully encapsulated into nanostructured lipid carriers. The developed NLC exhibited desirable parameters and was found to further improve immunomodulation efficacy. NLC has the potential to significantly enhance the efficacy of CT.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"23 ","pages":"Article 100244"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of drug-loaded niosomes fabricated by flow chemistry: A novel vortex tube reactor approach","authors":"Kampanart Huanbutta ,&nbsp;Kittipat Suwanpitak ,&nbsp;Possawee Ponlakorn ,&nbsp;Phatsawi Sonkongdang ,&nbsp;Isariya Kongklai ,&nbsp;Kunlathida Luangpraditkun ,&nbsp;Tistaya Semangoen ,&nbsp;Tanikan Sangnim","doi":"10.1016/j.onano.2025.100243","DOIUrl":"10.1016/j.onano.2025.100243","url":null,"abstract":"<div><div>Continuous flow technology has been increasingly applied in the fabrication of nanoparticle drug delivery systems due to its ability to provide enhanced process control, scalability, and improved uniformity in particle size. Therefore, this study aims to utilize a newly designed flow chemistry vortex tube reactor for the preparation of ketoconazole-loaded niosomes, with the goal of enhancing mixing efficiency and increasing production rates. The experiment was designed using a central composite design to investigate the effects of key preparation parameters, including total flow rate, surfactant concentration, and cholesterol content, on particle size, size distribution, zeta potential, entrapment efficiency, and drug loading percentage. The optimized formulation (Span 80 = 25 mg, Cholesterol = 50 mg) was achieved using a total flow rate of 20 mL/min. The resulting niosomes exhibited a particle size of 212.3 nm, a zeta potential of 40.2 mV, a polydispersity index of 0.282, an entrapment efficiency of 50.84 %, a drug loading of 0.58 %, and a productivity of 70.67 mg/min. Moreover, ketoconazole-loaded niosomes prepared using the newly designed flow chemistry vortex tube reactor demonstrated prolonged inhibition of <em>Candida albicans</em> growth compared to ketoconazole solution. A comparison with batch synthesis revealed that flow chemistry produces smaller particles with a narrower size distribution and significantly improved productivity. These findings indicate the potential for further development of the vortex reactor for industrial-scale production of nanovesicular drug delivery systems.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"23 ","pages":"Article 100243"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer activity of Kaempferia galanga L.–loaded polydopamine nanoparticles against colorectal cancer","authors":"Paweena Dana ,&nbsp;Anukul Taweechaipaisankul ,&nbsp;Yodsathorn Wongngam ,&nbsp;Suttipun Sungsuwan ,&nbsp;Walailuk Chonniyom ,&nbsp;Saksorn Klibaim ,&nbsp;Prattana Tanyapanyachon ,&nbsp;Monthira Rattanatayarom ,&nbsp;Onuma Phoraksa ,&nbsp;Udom Asawapirom ,&nbsp;Primana Punnakitikashem ,&nbsp;Duangporn Polpanich ,&nbsp;Nattika Saengkrit","doi":"10.1016/j.onano.2025.100242","DOIUrl":"10.1016/j.onano.2025.100242","url":null,"abstract":"<div><div><em>Kaempferia galanga</em> L. (KGL) is an aromatic ginger that has been used as a medicinal plant. Specifically, KGL possesses anti-inflammatory, antioxidant, anti-bacterial, and anti-cancer effects. However, the key component of KGL, ethyl <em>p</em>‑methoxy cinnamate (EPMC), is insoluble in water, resulting in low bioavailability. Hence, a nano-drug delivery system is used to enhance KGL activities. This study aimed to employ polydopamine (PDA) nanoparticles as a carrier for KGL delivery to improve its anti-cancer activity against colorectal cancer cells. PDA- and PDA nanoparticle–loaded KGL (PDA-KGL) were synthesized using a spontaneous oxidation process. The physicochemical properties of the PDA-KGL were characterized by dynamic light scattering methods. The anti-cancer activity of PDA-KGL was evaluated in HT-29, a colorectal cancer (CRC) cell line. Average hydrodynamic sizes of PDA and PDA-KGL were 236.2 ± 1.2 and 316.6 ± 2.0 nm, respectively, and the zeta potential of PDA and PDA-KGL were -23.0 ± 0.4 and -39.5 ± 0.6 mV, respectively. The morphology of PDA-KGL observed under TEM was spherical in shape. Anti-proliferative activity was monitored in HT-29 cells using MTT and 3D tumor spheroid assays. PDA-KGL strongly inhibited cell viability of HT-29 cells compared to free KGL and PDA treatments. PDA-KGL induced apoptosis in HT-29 cells as shown by an Annexin V binding assay. In addition, PDA-KGL suppressed the invasive ability of HT-29 cells compared to free KGL or PDA, which was determined by a transwell invasion assay. Taken together, it implies that PDA-KGL might be used as a nano-drug delivery approach for colorectal cancer treatment.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"23 ","pages":"Article 100242"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology for effective bovine mastitis treatment: Applications in biosensors and vaccines","authors":"Soheil Sadr ,&nbsp;Babak Khoramian ,&nbsp;Zahra Yousefsani ,&nbsp;Ahad Jafari Rahbar Alizadeh ,&nbsp;Abbas Rahdar ,&nbsp;Hassan Borji","doi":"10.1016/j.onano.2025.100238","DOIUrl":"10.1016/j.onano.2025.100238","url":null,"abstract":"<div><div>A dairy farm's most significant economic loss is caused by mastitis, one of the most common diseases in dairy cows. Mastitis occurs due to many different pathogens, such as bacteria, fungi, and algae, and antibiotics are the first line of treatment and control. Alternative methods are therefore being investigated to eradicate pathogenic microorganisms that cause mastitis. Nanotechnology is a rapidly growing field that can manufacture new materials at the nanoscale level and has tremendous potential to revolutionize the agri-food industry by offering new treatments for bovine mastitis. Considering the increasing ineffectiveness of current therapies for resistant bacteria, developing innovative products that combine nanotechnology may provide a sustainable animal health solution that can support the production of safe and high-quality food products while directly addressing a critical global problem such as antimicrobial resistance. Additionally, nanotechnology offers promising advancements in bovine mastitis research, with nanovaccines providing targeted and efficient delivery of antigens to enhance the cow's immune response and prevent the disease. On the other hand, nanobiosensors play a crucial role in the early detection of bovine mastitis, allowing for real-time monitoring of biomarkers and enabling prompt intervention, thus reducing economic losses and promoting animal welfare. Although metalic nanoparticles have the potential to offer great benefits in terms of treating bovine mastitis, there are several challenges related to their toxicity, stability, environmental safety, and production costs that need to be carefully examined and addressed in order to facilitate their use. Considering the potential of nanoparticles, these challenges can be successfully managed if applied to the right areas, which will be a new and efficient therapeutic method for improving bovine mastitis and reducing drug resistance in livestock farms, given the potential of nanoparticles. In summary, nanovaccines, nanodrugs, and nanobiosensors have great potential in managing bovine mastitis, offering targeted prevention, effective treatment, and early detection capabilities, respectively.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoformulation, characterization, and biological activity assays of extracts of Derris trifoliata Lour, a rutin-rich mangrove plant","authors":"Prattana Tanyapanyachon,&nbsp;Walailuk Chonniyom,&nbsp;Kananat Naksomboon,&nbsp;Jakarwan Yostawonkul,&nbsp;Paweena Dana,&nbsp;Udom Asawapirom,&nbsp;Nattika Saengkrit","doi":"10.1016/j.onano.2025.100236","DOIUrl":"10.1016/j.onano.2025.100236","url":null,"abstract":"<div><div><em>Derris trifoliata</em> Lour (DT) is a mangrove plant species with a promising anti-inflammatory effect. However, the application of DT as a topical anti-inflammatory agent is limited due to its poor bioavailability. Here, DT extract (DTE) from Kapoe District, Ranong Province, Thailand was selected due to its highest rutin over three investigated sources. The DTE was loaded into a nanoemulsion (NE), giving 74.33 ± 0.12 % and 75.29 ± 0.51 %, encapsulation efficiency of rutin and total phenolic content (TPC), respectively. The DTE-loaded NE (DTE-NE) exhibited a nano size (90.59 ± 1.27 nm) spherical, negative charge (-33.13 ± 0.12 mV), and narrow dispersity index (0.28 ± 0.01). The <em>in vitro</em> release profile of rutin and TPC from DTE-NE was slower compared to those from DTE. DTE-NE displayed a superior antioxidant capacity to DTE. Compared to DTE, DTE-NE treatment potentially lowered the inflammatory mediators (nitrite, interleukin-6, and tumor necrosis factor-alpha). Moreover, the physicochemical characteristics, antioxidant and anti-inflammatory activities remained unchanged after 1 month storage. In conclusion, the present study demonstrated the possible use of DTE-NE as a topical anti-inflammatory product.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100236"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrageenan bionanocomposite films incorporating Ag and Zn-Doped CeO₂ nanoparticles for active food packaging applications","authors":"Damar Rastri Adhika ,&nbsp;Gita Genecya ,&nbsp;Alvin Annayya Habibah ,&nbsp;An Naas Amalia Rahardja Putri ,&nbsp;Ubed Sonai Fahruddin Arrozi","doi":"10.1016/j.onano.2025.100234","DOIUrl":"10.1016/j.onano.2025.100234","url":null,"abstract":"<div><div>The increasing demand for sustainable and safe food packaging has led to the exploration of bio-based materials and advanced packaging technologies. This study investigates the incorporation of silver (Ag) and zinc (Zn) doped cerium oxide nanoparticles (CeO₂ NPs) into carrageenan-based bionanocomposite films to enhance their antimicrobial properties, mechanical strength, and hydrophobicity. The synthesis of CeO₂ NPs, doped with varying concentrations of Ag and Zn, was achieved using the green synthesis method with green tea extract as a reducing agent. Characterization techniques such as X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta potential analysis confirmed the successful doping and stability of the nanoparticles. The bionanocomposites were evaluated for their mechanical properties, water contact angle, and antibacterial activity against <em>Escherichia coli</em> and <em>Bacillus cereus</em>. Mechanical testing revealed that the addition of CeO₂ NPs, particularly Ag-doped CeO₂ NPs, significantly improved the tensile strength and Young's modulus of the bionanocomposites. Hydrophobicity assessments showed that Zn-doped CeO₂ NPs enhanced water resistance compared to Ag-doped CeO₂ NPs, making them more suitable for food packaging applications. Zn and Ag-doped CeO₂ NPs exhibited superior antibacterial activity compared to undoped CeO₂ NPs, with 20 wt% Ag-doped NPs showing the highest antibacterial activity compared to Amoxicillin as positive control and other variations. The study concludes that Zn and Ag-doped CeO₂ NPs are promising additives for developing effective and sustainable active food packaging materials.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100234"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing in Vitro anti-metastatic efficacy and deep penetration into tumor spheroid of docetaxel-loaded liposomes via size optimization for prostate cancer treatment","authors":"Saksorn Klibaim,&nbsp;Nutthanit Thumrongsiri,&nbsp;Natsorn Watcharadulyarat,&nbsp;Walailuk Chonniyom,&nbsp;Prattana Tanyapanyachon,&nbsp;Paweena Dana,&nbsp;Nattika Saengkrit","doi":"10.1016/j.onano.2024.100231","DOIUrl":"10.1016/j.onano.2024.100231","url":null,"abstract":"<div><div>Castration-resistant prostate cancer (CRPC) presents a formidable challenge due to its aggressiveness and limited treatment options. Loading the drug docetaxel (DTX) into liposomes is a potential alternative approach to improve its efficacy. Several studies have reported that size optimization can improve drug efficacy in other cancer models. Therefore, this study explored the potential of size-optimization of docetaxel-loaded liposomes (LDTX) to improve <em>in vitro</em> efficacy against CRPC. The impacts of LDTX size (&lt;100 nm and 100–200 nm) on cellular uptake, cytotoxicity in both monolayer and three-dimensional (3D) tumor spheroid models, and anti-metastatic effects were investigated. The results showed significant cellular internalization improvement with smaller LDTX, leading to better cytotoxicity in a monolayer cell culture than with larger LDTX. Moreover, smaller liposomes enabled deep penetration into the tumor spheroid, mimicking the tumor microenvironment and effectively eradicating cancer cells inside the spheroid. Interestingly, smaller liposomes also enhanced the anti-metastatic phenotype by inhibiting cancer cell invasion. The findings demonstrate that liposomes size is crucial in enhancing the efficacy of anti-cancer drugs. Therefore, size optimization is essential for developing highly effective formulations, requiring thorough investigation to identify the optimal liposomes size for specific applications.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100231"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol loaded zein nanoparticles for inhibiting proliferation of osteosarcoma cells: Synthesis, characterization, release profile, and cytotoxicity","authors":"Thanida Chuacharoen ,&nbsp;Carlos E. Astete ,&nbsp;Cristina M. Sabliov","doi":"10.1016/j.onano.2025.100237","DOIUrl":"10.1016/j.onano.2025.100237","url":null,"abstract":"<div><div>Resveratrol loaded zein nanoparticles (Res ZNPs) were synthesized to deliver resveratrol to bone cells for inhibiting the proliferation of osteosarcoma cells. Zein chemically cross-linked with alendronate as a bone-targeting agent using EDC and NHS chemistry was also synthesized and the cross-linking was confirmed using Fourier-Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Subsequently, Res ZNPs with and without alendronate were synthesized and characterized. The particles measured 273 to 294 nm with a narrow polydispersity index, and a zeta potential of -29 to -33 mV, respectively as evaluated by dynamic light scattering. The particles showed spherical morphology imaged by transmission electron microscopy and the entrapment efficiency and loading capacity were 63.0 % and 13.6 % for Res ZNPs and 69.1 % and 21.0 % for Res ZNPs with alendronate, respectively. Furthermore, the entrapped resveratrol of both systems was released in a three-phase manner under physiological condition (phosphate-buffered saline, PBS) at 37 °C over 24 h. Both systems exhibited suppression of osteosarcoma MG-63 cell proliferation and the inhibition rate was found slightly higher for targeted, Res ZNPs with alendronate. This research suggested that Res ZNPs conjugated with alendronate could be a candidate for effective bone-targeting delivery systems to inhibit the proliferation of osteosarcoma cells.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100237"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteogenic differentiation of mesenchymal stem cells in cell-laden culture of self-assembling peptide hydrogels","authors":"Faye Fouladgar ,&nbsp;Robert Powell ,&nbsp;Vishalakshi Irukuvarjula ,&nbsp;Akhila Joy ,&nbsp;Xiao Li ,&nbsp;Neda Habibi","doi":"10.1016/j.onano.2025.100235","DOIUrl":"10.1016/j.onano.2025.100235","url":null,"abstract":"<div><div>Mesenchymal stem cell (MSC) osteogenic differentiation requires scaffolds to support multiple stages of growth and differentiation signals. Fluorenyl-9-methoxycarbonyl diphenylalanine (Fmoc-FF) peptides self-assemble to create 3D nanofibers. Here, we cultured MSC in 2D and 3D Fmoc-FF layers to support their osteogenic differentiation. The stiffness of the hydrogels was tunable between 100 and 10,000 Pa which allows precise modulation of the cellular microenvironment. Scaffold stiffness impacted cell viability which softer scaffolds (100 Pa) favored higher viability. MSC formed spheroids in 3D hydrogel and showed spread morphology in 2D overlayers. Our results demonstrate that the Fmoc-FF 3D cultures significantly enhanced osteogenic differentiation, as evidenced by increased calcium deposition, elevated phosphatase activity, and the secretion of osteocalcin. We propose that the peptides provide integrin-binding sites that activate a cytoplasmic feedback loop essential for differentiation. These findings suggest that self-assembled Fmoc-FF peptide hydrogels, is a promising platform for bone tissue engineering applications.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100235"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143222762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic dissemination","authors":"Sara Micheli ,&nbsp;Caterina Piunti ,&nbsp;Elisa Varaschin ,&nbsp;Marianna Peditto ,&nbsp;Maria Luz Suarez ,&nbsp;Marco Sorgato ,&nbsp;Elisa Cimetta","doi":"10.1016/j.onano.2025.100233","DOIUrl":"10.1016/j.onano.2025.100233","url":null,"abstract":"<div><div>Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100233"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}