Non-coding RNA Research最新文献

{"title":"Expression of miR-15b-5p and toll-like receptor4 as potential novel diagnostic biomarkers for hepatitis C virus-induced hepatocellular carcinoma","authors":"Amal Ahmed Mohamed ,&nbsp;Noha Nagah Amer ,&nbsp;Noha Osama ,&nbsp;Wael Hafez ,&nbsp;Ali Elsaid Abdelrahman Ali ,&nbsp;Mahmoud Maamoun Shaheen ,&nbsp;Ayman Abd Alhady Alkhalegy ,&nbsp;Eman Alsayed Abouahmed ,&nbsp;Shamel Mohamed Soaida ,&nbsp;Lamees A. Samy ,&nbsp;Ahmed El-Kassas ,&nbsp;Ivan Cherrez-Ojeda ,&nbsp;Rehab R El-Awady","doi":"10.1016/j.ncrna.2024.12.003","DOIUrl":"10.1016/j.ncrna.2024.12.003","url":null,"abstract":"<div><h3>Objectives</h3><div>Globally, hepatocellular Carcinoma (HCC) ranks seventh in women's cancer and fifth in men's cancer. Early identification can minimize mortality and morbidity. MicroRNAs and Toll-like receptors have been suggested as potential new biomarkers for HCC; Therefore, we explored Toll-like receptor 4 (TLR-4) and miRNA 15b-5p as new non-invasive HCC biomarkers and early detection approaches.</div></div><div><h3>Methodology</h3><div>In this case-control study, four primary groups were formed from 400 patients who participated in this study: 100 hepatitis C (HCV) patients without cirrhosis or HCC, 100 HCV with cirrhosis patients, 100 HCC and HCV patients, and 100 healthy controls. The HCC diagnosis was confirmed according to the American Association for the Study of Liver Disease (AASLD) Practice Guidelines. Triphasic computed tomography was used to assess the HCC tumor size. Real-time PCR was used to analyze miRNA 15b-5p and Toll-like receptor 4 (TLR-4) expression profiles.</div></div><div><h3>Results</h3><div>Significant diagnostic performance was achieved by miRNA 15b-5p in differentiating the HCC group from the control group, with 90 % sensitivity and 88 % specificity (AUC] 0.935, p &lt; 0.001), while TLR-4 had moderate diagnostic performance with 85 % sensitivity and 86 % specificity (AUC:0.885, p &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>The ability of miR-15b-5p to recognize HCC was positive and it outperformed Toll-like receptor4. MiR-15b-5p has the potential to be a more precise and predictive biological marker for HCC than Toll-like receptor4. Future studies exploring different miRNAs and HCC cases from various etiologies are required to better understand the role of miRNAs in this disease and allow for more effective strategies.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"10 ","pages":"Pages 262-268"},"PeriodicalIF":5.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “MiRNA expression affects survival in patients with obstructive sleep apnea and metastatic colorectal cancer” [Non-coding RNA research 10 (2025) 91–97]","authors":"Piera Soccio ,&nbsp;Giorgia Moriondo ,&nbsp;Giulia Scioscia ,&nbsp;Pasquale Tondo ,&nbsp;Giuseppina Bruno ,&nbsp;Guido Giordano ,&nbsp;Roberto Sabato ,&nbsp;Maria Pia Foschino Barbaro ,&nbsp;Matteo Landriscina ,&nbsp;Donato Lacedonia","doi":"10.1016/j.ncrna.2024.09.010","DOIUrl":"10.1016/j.ncrna.2024.09.010","url":null,"abstract":"","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"10 ","pages":"Page 261"},"PeriodicalIF":5.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-210 overexpression increases pressure overload-induced cardiac fibrosis","authors":"G. Zaccagnini ,&nbsp;D. Baci ,&nbsp;S. Tastsoglou ,&nbsp;I. Cozza ,&nbsp;A. Madè ,&nbsp;C. Voellenkle ,&nbsp;M. Nicoletti ,&nbsp;C. Ruatti ,&nbsp;M. Longo ,&nbsp;L. Perani ,&nbsp;C. Gaetano ,&nbsp;A. Esposito ,&nbsp;F. Martelli","doi":"10.1016/j.ncrna.2025.01.009","DOIUrl":"10.1016/j.ncrna.2025.01.009","url":null,"abstract":"<div><div>Aortic stenosis, a common valvular heart disease, can lead to left ventricular pressure overload, triggering pro-fibrotic responses in the heart. miR-210 is a microRNA that responds to hypoxia and ischemia and plays a role in immune regulation and in cardiac remodeling upon myocardial infarction. This study investigated the effects of miR-210 on cardiac fibrosis caused by pressure overload.</div><div>Using a mouse model with inducible miR-210 over-expression, we subjected mice to transverse aortic constriction (TAC) to induce pressure overload. Mice with miR-210 over-expression developed eccentric hypertrophy, heightened expression of hypertrophic markers (Nppa and Nppb) and increased cross sectional area of cardiomyocytes, impacting the free wall of the left ventricle. These findings suggest that miR-210 worsens cardiac dysfunction. Furthermore, miR-210 over-expression led to a more robust and sustained inflammatory response in the heart, increased interstitial and perivascular fibrosis, and activation of myofibroblasts. miR-210 also promoted angiogenesis. <em>In vitro</em>, cardiac fibroblasts over-expressing miR-210 showed increased adhesion, wound healing and migration capacity.</div><div>Our results demonstrate that miR-210 contributes to adverse cardiac remodeling in response to pressure overload, including eccentric hypertrophy, inflammation, and fibrosis.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"12 ","pages":"Pages 20-33"},"PeriodicalIF":5.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair","authors":"Jiandong Zhang ,&nbsp;Lei Ma ,&nbsp;Limin He ,&nbsp;Quanxiao Xu ,&nbsp;Yan Ding ,&nbsp;Lidong Wang","doi":"10.1016/j.ncrna.2025.01.010","DOIUrl":"10.1016/j.ncrna.2025.01.010","url":null,"abstract":"<div><h3>Background</h3><div>While radiation-induced lung injury decreases quality of life and suppresses efficacy of radiotherapy, to date, the relationship between radiation-induced lung injury and repair remains unclear. Our previous studies revealed that TNFRSF10B-RIPK1/RIPK3-MLKL signaling induces necroptosis of alveolar epithelial cells and potentiates radiation-induced lung injury. We also found that microRNA-541-3p is differentially expressed in radiation-damaged lungs. The connection between microRNA-541-3p, TNFRSF10B signaling, and TGFβ1 signaling is also unclear.</div></div><div><h3>Objective</h3><div>This study was performed to explore the regulatory effects of microRNA-541-3p on TNFRSF10B and TGFβ1 signaling.</div></div><div><h3>Methods</h3><div>Mouse alveolar epithelial cells were transfected with a vector expressing microRNA-541-3p to regulate expression of target genes. Flow cytometry, polymerase chain reaction, and western blotting were used to analyze cell necroptosis, target gene expression, and target protein expression, respectively.</div></div><div><h3>Results</h3><div>Overexpression of microRNA-541-3p positively regulated TNFRSF10B-RIPK1/RIPK3-MLKL signaling through Rac2 to induce cell necroptosis. MicroRNA-541-3p negatively regulates Rac2. MicroRNA-541-3p and Rac2 regulate the expression of Tgf-beta1 and its encoded proteins.</div></div><div><h3>Conclusions</h3><div>The Rac2 gene synchronously regulates TNFRSF10B-RIPK1/RIPK3-MLKL and TGFβ1 signaling. MicroRNA-541-3P/Rac2 act as mediators of radiation damage and repair signaling.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"12 ","pages":"Pages 10-19"},"PeriodicalIF":5.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LURAP1L-AS1 long noncoding RNA promotes breast cancer progression and associates with poor prognosis","authors":"Radhakrishnan Vishnubalaji ,&nbsp;Dania Awata ,&nbsp;Nehad M. Alajez","doi":"10.1016/j.ncrna.2025.01.006","DOIUrl":"10.1016/j.ncrna.2025.01.006","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) are emerging as critical regulators of cancer biology, yet their roles in breast cancer, particularly in triple-negative breast cancer (TNBC), remain incompletely understood. Through a custom siRNA library screen targeting TNBC-associated lncRNAs in MDA-MB-231 and BT-549 TNBC cell models, we identified LURAP1L-AS1 as a key modulator of TNBC progression. Survival analysis of TNBC patients demonstrated a significant association between elevated LURAP1L-AS1 expression and poor clinical outcomes.</div><div>LURAP1L-AS1 knockdown significantly impaired colony formation and organoid growth of TNBC models, associated with increased apoptosis thus highlighting its role in promoting tumorigenicity. RNA sequencing of LURAP1L-AS1-depleted cells revealed dysregulation of pathways related to cell proliferation, apoptosis, migration, and RNA processing. Bioinformatics analysis predicted LURAP1L-AS1 to function as a competitive endogenous RNA (ceRNA), sponging key microRNAs, such as miR-7a-5p, miR-101-3p, miR-181a-5p, and miR-27a-3p, thereby modulating oncogenes including EZH2, MCL1, and KRAS, which are linked to increased cancer cell survival, proliferation, and metastasis.</div><div>In addition to its role in TNBC, correlation analysis using breast cancer patient datasets revealed a significant association between LURAP1L-AS1 and ESR1 expression, suggesting its broader impact across breast cancer subtypes. Concordantly, LURAP1L-AS1 depletion inhibited estrogen receptor-positive (ER+) MCF7 breast cancer cells colony formation and organotypic growth.</div><div>Our findings establish LURAP1L-AS1 as a functional lncRNA that promotes breast cancer progression, highlighting its potential for use in RNA-based therapies for breast cancer.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"12 ","pages":"Pages 1-9"},"PeriodicalIF":5.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the interaction between intestinal flora and microRNA in pelvic inflammatory diseases","authors":"Shuhan Dong ,&nbsp;Yunpeng Du ,&nbsp;Haiyang Wang ,&nbsp;Wenhan Yuan ,&nbsp;Wenxia Ai ,&nbsp;Li Liu","doi":"10.1016/j.ncrna.2025.01.007","DOIUrl":"10.1016/j.ncrna.2025.01.007","url":null,"abstract":"<div><div>Pelvic inflammatory disease (PID) is a common infectious disease of the female upper reproductive tract, and its pathological basis is immune inflammatory response. The imbalance of gut microflora (GM) may lead to the development of inflammatory process. A large number of studies have shown that fecal microbiota transplantation, probiotics, bacteria, prebiotics, and dietary intervention may play a potential role in remodeling GM and treating diseases. MicroRNAs (miRNAs) are involved in cell development, proliferation, apoptosis and other physiological processes. In addition, they play an important role in the inflammatory process, participating in the regulation of proinflammatory and anti-inflammatory pathways. Differences in miRNA profiles may be PID diagnostic tools and serve as prognostic markers of the disease. The relationship between miRNA and GM has not been fully elucidated. Recent studies have shown the role of miRNA in the regulation and induction of GM dysbiosis. In turn, microbiota can regulate the expression of miRNA and improve the immune status of the body. Therefore, this review aims to describe the interaction between GM and miRNA in PID, and to find potential precise targeted therapy for PID.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"11 ","pages":"Pages 303-312"},"PeriodicalIF":5.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143129810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients","authors":"Salman M. Toor ,&nbsp;Eman K. Aldous ,&nbsp;Aijaz Parray ,&nbsp;Naveed Akhtar ,&nbsp;Yasser Al-Sarraj ,&nbsp;Abdelilah Arredouani ,&nbsp;Ghulam Jeelani Pir ,&nbsp;Sajitha V. Pananchikkal ,&nbsp;Omar El-Agnaf ,&nbsp;Ashfaq Shuaib ,&nbsp;Nehad M. Alajez ,&nbsp;Omar M.E. Albagha","doi":"10.1016/j.ncrna.2025.01.005","DOIUrl":"10.1016/j.ncrna.2025.01.005","url":null,"abstract":"<div><h3>Background</h3><div>Stroke refers to an abrupt neurological deficit, caused by an acute focal injury of the central nervous system via infarction or hemorrhage due to impaired vascularity, and remains among the leading causes of disability and death worldwide. Stroke is often preceded by an episode of neuronal deficit termed transient ischemic attack (TIA), which presents an effective opportunity for mitigating the risk of an eminent acute ischemic stroke (AIS). Circulating non-coding RNAs (ncRNAs) have emerged as important biomarkers for stroke, but PIWI-interacting RNAs (piRNAs), a class of small regulatory ncRNAs, have not been previously explored as diagnostic or prognostic biomarkers for stroke.</div></div><div><h3>Methods</h3><div>We conducted comprehensive circulating piRNA profiling of AIS and TIA patients using RNA-seq on serum samples collected within 24 h of clinical diagnosis. The study cohort was divided into discovery and cross-validation datasets to identify replicated piRNAs using stringent analysis cut-offs. The expression levels of the panel of differentially regulated piRNAs between AIS and TIA patients were also compared with healthy controls.</div></div><div><h3>Results</h3><div>We identified a panel of 10 differentially regulated piRNAs between AIS and TIA patients; hsa-piR-28272, -piR-32972, -piR-28247, -piR-24553, -piR-24552, -piR-28275, -piR-28707 and -piR-32882 were upregulated, while hsa-piR-23058 and -piR-23136 were downregulated in AIS patients. Moreover, these 10 piRNAs were also differentially expressed in AIS patients compared to healthy controls. In addition, we investigated the potential gene targets of the dysregulated piRNAs and their plausible involvement in pathophysiological processes affected in stroke.</div></div><div><h3>Conclusions</h3><div>The imbalances in the circulating piRnome of AIS and TIA patients presented herein provide important insights into the roles of piRNAs following ischemic brain injury and potentially provide opportunities to mitigate stroke-induced mortality and morbidity.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"11 ","pages":"Pages 294-302"},"PeriodicalIF":5.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143129809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNAs in humans: Classification, genomic organization and function","authors":"Barbara Chodurska ,&nbsp;Tanja Kunej","doi":"10.1016/j.ncrna.2025.01.004","DOIUrl":"10.1016/j.ncrna.2025.01.004","url":null,"abstract":"<div><div>Long non-coding RNAs (lncRNAs) regulate numerous biological functions in animals. Despite recent advances in lncRNA research, their structural and functional annotation and classification remain an ongoing challenge. This review provides a comprehensive overview of human lncRNAs, highlighting their genomic organization, mode of action and role in physiological and pathological processes. Subgroups of lncRNA genes are discussed using representative examples and visualizations of genomic organization. The HUGO Gene Nomenclature Committee (HGNC) categorizes lncRNAs into nine subgroups: (1) microRNA non-coding host genes, (2) small nucleolar RNA non-coding host genes, (3) long intergenic non-protein coding RNAs (LINC), (4) antisense RNAs, (5) overlapping transcripts, (6) intronic transcripts, (7) divergent transcripts, (8) long non-coding RNAs with non-systematic symbols and (9) long non-coding RNAs with FAM root systems. Circular RNAs (circRNAs) are a separate class that shares some characteristics with lncRNAs and are divided into exonic, intronic and intronic-exonic types. LncRNAs act as molecular signals, decoys, scaffolds and sponges for microRNAs and often function as competing endogenous RNAs (ceRNAs). LncRNAs are involved in various physiological and pathological processes, such as cell differentiation, p53-mediated DNA damage response, glucose metabolism, inflammation and immune functions. They are associated with several diseases, including various types of neoplasms, Alzheimer's disease and autoimmune diseases. A clear classification system for lncRNA is essential for understanding their biological role and for facilitating practical applications in biomedical research. Future studies should focus on drug development and biomarker discovery. As important regulators of various biological processes, lncRNAs represent promising targets for innovative therapies.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"11 ","pages":"Pages 313-327"},"PeriodicalIF":5.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143129811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA-mRNA regulatory network reveals key lncRNAs tightly associated with preterm labor and premature rupture of membranes","authors":"Guangqiong Yang,&nbsp;Wenjin Qi","doi":"10.1016/j.ncrna.2025.01.002","DOIUrl":"10.1016/j.ncrna.2025.01.002","url":null,"abstract":"<div><div>Premature rupture of membranes (PROM) and preterm birth (PTB) are significant pregnancy complications, accounting for approximately one-third of PTB, often preceded by preterm PROM (PPROM). The underlying causes of PROM and PTB are multifaceted and not fully understood. Long non-coding RNAs (lncRNAs) have emerged as pivotal elements in the molecular landscape of PPROM. In our study, we analyzed fetal membrane samples from Term labor (TL), PROM, PTB, and PPROM groups using transcriptome sequencing to identify differentially expressed genes, including both lncRNAs and mRNAs. Our findings highlighted a subset of lncRNAs, BBOX1-AS1, VIM-AS1, XLOC-031812 and AC106706.1 as potentially influential in the pathophysiology of PROM and PTB. Co-expression analyses further revealed that the target genes regulated by these lncRNAs were significantly implicated in pregnancy progression and embryonic placental development. These insights underscored the importance of the lncRNA-mRNA axis in the onset and progression of PROM and PTB, offering new avenues for understanding the molecular mechanisms underlying these conditions. Our research not only contributes to the elucidation of lncRNA mediated regulatory mechanisms in PROM and PTB, but also holds promise for improving preventative and therapeutic strategies, ultimately safeguarding maternal and infant well-being.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"11 ","pages":"Pages 273-280"},"PeriodicalIF":5.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143129197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of MALAT1 facilitates ROS accumulation via the Keap1/HO-1 pathway to enhance photodynamic therapy in secondary hyperparathyroidism","authors":"Ying Wen ,&nbsp;Yitong Li ,&nbsp;Danhua Zhang ,&nbsp;Ziru Liu ,&nbsp;Hong Liu ,&nbsp;Xiejia Li ,&nbsp;Wei Wu ,&nbsp;Liyun Zeng ,&nbsp;Qiongyan Zou ,&nbsp;Wenjun Yi","doi":"10.1016/j.ncrna.2024.12.001","DOIUrl":"10.1016/j.ncrna.2024.12.001","url":null,"abstract":"<div><div>The prevalence of secondary hyperparathyroidism (SHPT) in advanced chronic kidney disease (CKD) exceeds 80 %. Our previous study indicated that photodynamic therapy (PDT) has potential for treating SHPT. Long noncoding RNA (lncRNA) is involved in various oxidative stress and apoptotic processes, but the molecular mechanism remains unreported. In this study, we found that PDT induced apoptosis in SHPT through reactive oxygen species (ROS) accumulation. The expression of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and heme oxygenase 1 (HO-1) within SHPT was upregulated after PDT. Inhibition of MALAT1 increased PDT-induced ROS, which promoted the apoptosis. Pearson correlation analysis confirmed that there was a positive correlation between MALAT1 and HO-1, and MALAT1 inhibition down-regulated HO-1, whereas concomitant overexpression of HO-1 was able to eliminate the PDT-induced ROS and inhibit apoptosis. The direct binding of MALAT1 to Kelch-like ECH-associated protein 1 (Keap1) protein was confirmed by high-throughput sequencing, RNA pulldown, silver staining and western blotting assays. Si-Keap1 was able to rescue the down-regulation of HO-1 caused by MALAT1 inhibition, restoring the elimination of ROS by HO-1 and attenuating the effect of PDT. In addition, PDT effectively reduced parathyroid hormone (PTH) secretion in SHPT rats, and this effect was further enhanced in combination with MALAT1 inhibitors. Overall, MALAT1 activates downstream HO-1 expression by binding to Keap1, thereby reducing ROS and inhibiting apoptosis, which in turn mediates PDT resistance in SHPT. Inhibition of MALAT1 significantly enhanced the efficacy of PDT, suggesting a potential therapeutic target for improving PDT for SHPT outcomes.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"11 ","pages":"Pages 249-261"},"PeriodicalIF":5.9,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}