Applied In Vitro Toxicology最新文献_第2页

Apoptotic Cell Death in Cardiomyocytes Induced by Hypoxia from Cobalt Chloride Is Hastened by SGLT-1 Inhibition 抑制SGLT-1可加速氯化钴缺氧诱导心肌细胞凋亡

Applied In Vitro Toxicology Pub Date : 2023-09-01 DOI: 10.1089/aivt.2023.0006

Abhinav Kanwal, Lu Liu, Puneet Kumar Bansal, Hemant Kumar, Shailendra Pratap Singh

{"title":"Apoptotic Cell Death in Cardiomyocytes Induced by Hypoxia from Cobalt Chloride Is Hastened by SGLT-1 Inhibition","authors":"Abhinav Kanwal, Lu Liu, Puneet Kumar Bansal, Hemant Kumar, Shailendra Pratap Singh","doi":"10.1089/aivt.2023.0006","DOIUrl":"https://doi.org/10.1089/aivt.2023.0006","url":null,"abstract":"Introduction: Myocardial ischemia is responsible for the deaths of millions of people every year. Cardiac hypoxia reduces the efficiency with which the heart muscle pumps blood. When one or more of the coronary arteries abruptly and severely narrows or closes off, this is known as an acute coronary syndrome (ACS). Ischemia of the heart muscle can also cause potentially fatal arrhythmias. More information about this topic is required. Methods: The effects of SGLT-1 inhibition were studied using a different disease model, the cobalt chloride (CoCl2) hypoxia paradigm. The MTT assay was used to examine the effects of CoCl2 with and without Phlorizin (PZ) on glucose uptake, caspase activity, and metabolic/cytotoxic activities in SGLT-1 overexpressed H9C2 cells. Both SGLT-1 siRNA silencing and PZ treatment of SGLT-1 overexpressed neonatal rat cardiomyocytes were studied. Results and Discussion: Using flow cytometry, we were able to distinguish between metabolically active (PI-stained) and inactive (annexin-stained) live cells, as well as apoptotic (annexin-stained) and necrotic (PI-stained) cells. Caspase 3, 9, bcl-2, HIF-1a, and SGLT-1 expression, as well as oxidative stress, were examined using Western blotting. H9C2 cells showed increased caspase 3 and 9 activity in the CoCl2 group compared to the control, and these increases were further amplified by PZ cotreatment. PZ did not counteract CoCl2's effects of decreased glucose absorption and MTT activity. Conclusion: PZ increased cardiomyocyte apoptosis and decreased metabolic quiescent cells. PZ had no effect on the oxidative stress and necrosis that CoCl2 caused. CoCl2-induced SGLT-1 reduction leads to rapid apoptotic cell death.","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135388328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kombucha Mushroom Extract: Anticancer, Antioxidant, and Antimicrobial Properties 康普茶蘑菇提取物:抗癌，抗氧化和抗菌特性

Applied In Vitro Toxicology Pub Date : 2023-07-19 DOI: 10.1089/aivt.2023.0005

F. Motafeghi, P. Mortazavi, Romina Shahsavari, Maryam Shaker, Hamideh Mohammadi-Berenjestanaki, M. Shokrzadeh

引用次数: 0

PeakAUC: An R Shiny Application for Area Under the Peak Calculations and Nitric Oxide Analysis 峰下面积计算和一氧化氮分析的R闪亮应用

Applied In Vitro Toxicology Pub Date : 2023-06-01 DOI: 10.1089/aivt.2023.0002

Gregory Pappas, Andrew Gow

引用次数: 0

Assessment of the Predictivity of DIO1-SK Assay to Investigate DIO1 Inhibition in Human Liver Microsomes DIO1-SK测定对人肝微粒体中DIO1抑制作用的预测性评估

Applied In Vitro Toxicology Pub Date : 2023-06-01 DOI: 10.1089/aivt.2022.0016

A. Weber, B. Birk, Varun Giri, S. Hoffmann, K. Renko, S. Coecke, S. Schneider, D. Funk-Weyer, R. Landsiedel

引用次数: 0

Expression Analysis of Exosomal MicroRNAs in Chlorpromazine Treated Human Hepatic Spheroids: A Promising Tool for Novel Drug-Induced Liver Injury Biomarker Discovery 氯丙嗪治疗的人肝球体中外泌体MicroRNAs的表达分析:发现新型药物性肝损伤生物标志物的一个有希望的工具

Applied In Vitro Toxicology Pub Date : 2023-05-25 DOI: 10.1089/aivt.2023.0004

A. Borgström, B. Filippi, P. Hewitt, A. Wolf, M. Gebauer

引用次数: 0

Influence of Uric Acid on Erythrocytes Subjected to H2O2-Induced Oxidative Stress 尿酸对h2o2诱导的红细胞氧化应激的影响

Applied In Vitro Toxicology Pub Date : 2023-04-12 DOI: 10.1089/aivt.2023.0001

M. Pallavi, G. S. S. Nithindran, R.V. Ranjithvishal, N. S. Laavanyaa, C. Bhat, Prerana Banerjee, Aastha Choudhary, Vani Rajashekaraiah

引用次数: 2

Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays. 全氟羧酸和磺酸对虹鳟雌激素受体结合和肝切片Vtg mRNA表达的影响

Applied In Vitro Toxicology Pub Date : 2023-03-17 DOI: 10.1089/aivt.2022.0013

Mark A Tapper, Jeffrey S Denny, Barbara R Sheedy, Ben Johnson, Richard C Kolanczyk

{"title":"Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays.","authors":"Mark A Tapper, Jeffrey S Denny, Barbara R Sheedy, Ben Johnson, Richard C Kolanczyk","doi":"10.1089/aivt.2022.0013","DOIUrl":"10.1089/aivt.2022.0013","url":null,"abstract":"Perfluoroalkylated substances (PFAS) such as carboxylic acids, and sulfonic acids were manufactured in high quantities and are ubiquitous environmental contaminants. These chemicals persist in the environment and tend to bioaccumulate. In the current study, the estrogenic potential of a series of perfluoro carboxylic acids and select perfluoro sulfonic acids were assessed in an in vitro rainbow trout estrogen receptor (rtER) binding assay and an ex vivo rtER dependent vitellogenin (Vtg) expression rainbow trout liver slice assay. Perfluoro carboxylic acids with perfluoroalkyl chain lengths of four to six did not significantly bind to the rtER or induce Vtg expression in liver slices. Perfluoro carboxylic acids with chain lengths of seven to ten, and sulfonic acids with seven and eight carbon chains bound to the rtER, but with low relative binding affinities. While affinity for the rtER increased with increasing chain length the highest affinity measured was only 0.0025% relative to the endogenous hormone 17ß-estradiol at 100%. Both the eight-carbon carboxylic acid and eight-carbon sulfonic acid induced Vtg expression in ex vivo liver slices. However, toxicity did not allow expression to achieve maximum efficacy relative to estradiol.","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":"13-22"},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11151740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47285964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Rabbit Induced Pluripotent Stem Cell-Derived Cardiomyocytes to Investigate Drug-Induced Fetal Heart Malformations 利用兔诱导的多能干细胞来源的心肌细胞研究药物诱导的胎儿心脏畸形

Applied In Vitro Toxicology Pub Date : 2023-03-06 DOI: 10.1089/aivt.2022.0017

John T. Szilagyi, K. Kolaja

引用次数: 0

Acknowledgment of Reviewers 2022 审稿人致谢

Applied In Vitro Toxicology Pub Date : 2023-03-01 DOI: 10.1089/aivt.2022.29032.ack

{"title":"Acknowledgment of Reviewers 2022","authors":"","doi":"10.1089/aivt.2022.29032.ack","DOIUrl":"https://doi.org/10.1089/aivt.2022.29032.ack","url":null,"abstract":"Applied In Vitro ToxicologyVol. 9, No. 1 AcknowledgmentFree AccessAcknowledgment of Reviewers 2022Published Online:17 Mar 2023https://doi.org/10.1089/aivt.2022.29032.ackAboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail Critical evaluations of articles by expert reviewers make a vital contribution to ensuring the high quality of a journal's content. The editorial leadership of Applied In Vitro Toxicology is very grateful for the support of the highly qualified peer reviewers who have dedicated their time and effort to reviewing our articles. We would like to show our appreciation by thanking the following individuals for their assistance with the review of articles for the journal in 2022.**Data through November 30, 2022.Raha AminiMarcelo ArboAnita BoelenChad DeisenrothEdvande dos Santos FilhoChantra EskesStephen FergusonLeonardo FranchiAnne GourmelonJosé GranjeiroRhiannon HardwickJulia HerbertHelena HogbergJian JiangAnne KahruHajime KojimaVirkas KumarGhislaine LacroixK. LeeDavid LehmannBrett LidburyLarissa Matuda MacedoShadab MdMartha MooreFiona MurphyClive RoperTimothy ShaferMaqsood A. SiddiquiDavid ThorneErwin van VlietRoman WieczorekFiguresReferencesRelatedDetails Volume 9Issue 1Mar 2023 InformationCopyright 2023, Mary Ann Liebert, Inc., publishersTo cite this article:Acknowledgment of Reviewers 2022.Applied In Vitro Toxicology.Mar 2023.35-35.http://doi.org/10.1089/aivt.2022.29032.ackPublished in Volume: 9 Issue 1: March 17, 2023PDF download","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135130907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Validation is the Key to Furthering In Vitro Toxicology 验证是推进体外毒理学的关键

Applied In Vitro Toxicology Pub Date : 2023-03-01 DOI: 10.1089/aivt.2023.29033.editorial

Andrew Gow

引用次数: 0