Applied In Vitro Toxicology最新文献

{"title":"In Vitro Assessment of Translocation and Toxicological Effects of Nicotine and Ethyl Maltol from e-Cigarettes Using Air–Liquid Interface-Cultured Bronchial Epithelial Cells","authors":"Y. Staal, Eric Gremmer, G. Duijm, E. Duistermaat, Paul Fokkens, D. Lensen, H. Hodemaekers, Lou Maas, Alexander Remels, R. Talhout","doi":"10.1089/aivt.2023.0019","DOIUrl":"https://doi.org/10.1089/aivt.2023.0019","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"51 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140269676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Assessing Experimental Uncertainty in Defined Approaches: Borderline Ranges for In Chemico and In Vitro Skin Sensitization Methods Determined from Ring Trial Data, by Kolle, et al. Appl In Vitro Toxicol 2021;7(3):102–111; doi: 10.1089/aivt.2021.0003","authors":"","doi":"10.1089/aivt.2021.0003.correx","DOIUrl":"https://doi.org/10.1089/aivt.2021.0003.correx","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"87 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140275805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Toxicokinetic Information for Setting Concentrations of In Vitro Toxicity Tests and for Interpreting Their Results: A Proposed Workflow","authors":"R. Landsiedel, B. Birk, Philipp Demuth, E. Fabian, Nicola J. Hewitt, H. Hollnagel, Julia Scheel","doi":"10.1089/aivt.2023.0018","DOIUrl":"https://doi.org/10.1089/aivt.2023.0018","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140271370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the VITROCELL Mammalian 48 Exposure Module Using In Vitro Cytotoxicity via the Neutral Red Uptake Assay in Chinese Hamster Ovary Cells Following Exposure to Whole Smoke from Combustible Cigarettes","authors":"Brian M. Keyser, R. Leverette, M. Hollings, E. Rothwell, Randy A. Weidman, Carlton J. Bequette, Kristen G. Jordan","doi":"10.1089/aivt.2023.0013","DOIUrl":"https://doi.org/10.1089/aivt.2023.0013","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"31 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139017595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Resveratrol on Cytotoxicity, Genotoxicity, and Oxidative Stress Caused by Cyclophosphamide and Methotrexate in Bone Marrow Stem Cell Line and Blood Lymphocytes of the Rat","authors":"F. Motafeghi, P. Mortazavi, Javad Zabihi, Asiye Soroori, Pouria Samadi Mojaveri, Mohammad Shokrzadeh","doi":"10.1089/aivt.2023.0008","DOIUrl":"https://doi.org/10.1089/aivt.2023.0008","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"423 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138993109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of p-Hydroxybenzoic Acid on Erythrocytes Exposed to Oxidative Stress","authors":"Magdaline Christina Rajanand, Carl Hsieh, Piyali Jana, Akshaya Panamkulangara, Sneha Jayamma Hanumanthareddy, Faseeha Akbar Shaikh, Sriraksha Srinath, Vani Rajashekaraiah","doi":"10.1089/aivt.2023.0012","DOIUrl":"https://doi.org/10.1089/aivt.2023.0012","url":null,"abstract":"Background: Oxidative stress (OS) causes deleterious changes in erythrocytes leading to hemolysis and macrophage-mediated removal from the circulation. Antioxidants have proven to protect the erythrocytes from induced OS in vitro. Hydroxybenzoic acid (HBA), a phenolic compound, exhibits free radical scavenging properties. Therefore, this study was conducted to evaluate the role of HBA on erythrocytes subjected to OS. Materials and Methods: Erythrocytes isolated from the blood collected from male Wistar rats were grouped into controls and experimental groups—samples with 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), HBA10 (10 mM HBA), HBA30 (30 mM HBA), and samples with combination of AAPH and HBA. The markers of OS and antioxidant status were determined. Statistical analyses were performed using GraphPad Prism 8 Software. Results: Hemoglobin decreased in HBA and AAPH + HBA groups against their respective controls. Conjugate dienes increased in HBA10 against control. Thiobarbituric acid reactive substances (TBARSs) increased in AAPH + HBA10 against AAPH. Superoxide dismutase activity elevated in the AAPH + HBA10 group compared with AAPH. Catalase levels increased in HBA10 against control. Conclusion: p-Hydroxybenzoic acid has proved to be beneficial in scavenging the free radicals generated by AAPH as reflected in the results of hemolysis, sulfhydryls, and TBARS and antioxidant enzymes.","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135291159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of the Synthetic Cannabinoid AB-CHMINACA on the Roles of Vascular Endothelial Growth Factor, Angiopoietin-1, and Angiopoietin-2 in Brain Angiogenesis","authors":"L. Al-Eitan, Hana Abu Kharmah","doi":"10.1089/aivt.2023.0003","DOIUrl":"https://doi.org/10.1089/aivt.2023.0003","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47155298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caffeine Expands the Cisplatin-Induced Cell Death by Strengthening <i>eIF2α/ATF4/CHOP</i> Signaling and Blocking Autophagic Flux in Human Prostate Cancer Cells","authors":"Yalcin Erzurumlu, Hatice Kubra Dogan, Deniz Catakli","doi":"10.1089/aivt.2023.0010","DOIUrl":"https://doi.org/10.1089/aivt.2023.0010","url":null,"abstract":"Purpose: Platinum-based compound cisplatin is widely used in various tumors, whereas its usage against many types of cancer, including prostate, is limited because of poor targeting, dose-limiting toxicity, and acquired resistance. Caffeine (Cfn) is a purine alkaloid compound and is widely consumed. Until now, a growing body of data has shown that Cfn has anticancer properties and is suitable for use with conventional chemotherapeutics. Thus, there is a need to develop more effective therapeutic approaches. Herein, we assessed the possible synergistic effect of Cfn with cisplatin in prostate cancer cells. Materials and Methods: Cell proliferation was evaluated by WST-1 assay. Autophagy, ubiquitin-proteasome system, unfolded protein response, endoplasmic reticulum stress, apoptosis, epithelial–mesenchymal transition (EMT), and androgenic signal-related numerous protein levels were analyzed by immunoblotting. Nucleus formation and cell death-related alterations were examined by immunofluorescence microscopy. Colonial growth was evaluated with a colony formation assay. Results: We found that androgen-sensitive prostate adenocarcinoma LNCaP cells were more sensitive to Cfn than metastatic prostate cancer Du145 cells and healthy prostatic cells PNT1A. Cousage of cisplatin and Cfn exhibited an expanded anticancer effect by inducing eukaryotic initiation factor 2α (eIF2α)/activating transcription factor 4 (ATF4)/C/EBP Homologous Protein (CHOP) signaling and negatively regulating autophagic flux. Moreover, Cfn displayed anticancer effects, and cotreatment with cisplatin gradually enhanced these properties. Furthermore, Cfn reduced the EMT and c-Myc expression. Also, androgenic signaling was strongly restricted by the cotreatment of Cfn and cisplatin. Conclusion: Our results suggest that combining the usage of cisplatin with Cfn treatment may offer an advanced therapeutic approach to prostate cancer.","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135388165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Interview with Nobel Laureate Louis J. Ignarro","authors":"Andrew J. Gow","doi":"10.1089/aivt.2023.0017","DOIUrl":"https://doi.org/10.1089/aivt.2023.0017","url":null,"abstract":"Applied In Vitro ToxicologyVol. 9, No. 3 InterviewAn Interview with Nobel Laureate Louis J. IgnarroInterview by Andrew J. GowInterview by Andrew J. Gowhttps://orcid.org/0000-0003-0876-5158Department of Pharmacology & Toxicology, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey, USA.Search for more papers by this authorPublished Online:19 Sep 2023https://doi.org/10.1089/aivt.2023.0017AboutSectionsView articleView Full TextPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail View articleFiguresReferencesRelatedDetails Volume 9Issue 3Sep 2023 InformationCopyright 2023, Mary Ann Liebert, Inc., publishersTo cite this article:Interview by Andrew J. Gow.An Interview with Nobel Laureate Louis J. Ignarro.Applied In Vitro Toxicology.Sep 2023.125-128.http://doi.org/10.1089/aivt.2023.0017Published in Volume: 9 Issue 3: September 19, 2023PDF download","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135388338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosalind Franklin Society Proudly Announces the 2022 Award Recipient for <i>Applied In Vitro Toxicology</i>","authors":"Judy Strickland","doi":"10.1089/aivt.2023.29034.rfs2022","DOIUrl":"https://doi.org/10.1089/aivt.2023.29034.rfs2022","url":null,"abstract":"Applied In Vitro ToxicologyVol. 9, No. 3 AnnouncementFree AccessRosalind Franklin Society Proudly Announces the 2022 Award Recipient for Applied In Vitro ToxicologyJudy StricklandJudy StricklandInotiv, Inc. Morrisville, NC, USASearch for more papers by this authorPublished Online:19 Sep 2023https://doi.org/10.1089/aivt.2023.29034.rfs2022AboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail The Rosalind Franklin Society (RFS), in partnership with Mary Ann Liebert, Inc., publishers, enthusiastically congratulate our distinguished recipient of the 2022 annualRFS Award in Sciencefor this journal, which recognizes the outstanding research and published work of women and underrepresented minority scientists, physicians, and engineers.Judy Strickland, et al., “Application of Defined Approaches to Assess Skin Sensitization Potency of Isothiazolinone Compounds,” Applied In Vitro Toxicology 8, no. 4 (December 2022): 117–128, http://doi.org/10.1089/aivt.2022.0014.AbstractIsothiazolinones are widely used as antimicrobial preservatives in cosmetics and in consumer and industrial products to control bacteria, fungi, and algae. While they are effective biocides, they have the potential to produce skin irritation and sensitization, which poses a human health hazard. In this project, we evaluated non-animal defined approaches for skin sensitization that can provide point-of-departure estimates for use in quantitative risk assessment for isothiazolinones. The skin sensitization potential of six isothiazolinones was evaluated using three internationally harmonized non-animal test methods. Results from these test methods were then applied to two versions of the Shiseido Artificial Neural Network defined approach. Sensitization hazard or potency predictions were compared to those of the in vivo murine local lymph node assay (LLNA). The non-animal methods produced skin sensitization hazard and potency classifications concordant with those of the LLNA. Potency values generated by the defined approaches had less variability than those from the LLNA, and confidence limits from the defined approaches overlapped those of the LLNA for most substances. The application of in silico models to non-animal skin sensitization data is a promising data integration procedure for defined approaches to support hazard and potency classification and quantitative risk assessment.BiosketchDr. Judy Strickland is a principal predictive toxicologist at Inotiv, Inc., with 22 years of experience in evaluating alternative test methods that reduce, refine, or replace animals in regulatory toxicity test methods. Dr. Strickland has both coordinated and evaluated national and international validation studies to assess the performance of non-animal methods for assessing chemical toxicity. Her efforts in evaluating non-animal methods for the assessment of chemical skin sensitizers c","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135388008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}