对羟基苯甲酸对氧化应激红细胞的影响

Q2 Health Professions

Applied In Vitro Toxicology Pub Date : 2023-11-09 DOI:10.1089/aivt.2023.0012

Magdaline Christina Rajanand, Carl Hsieh, Piyali Jana, Akshaya Panamkulangara, Sneha Jayamma Hanumanthareddy, Faseeha Akbar Shaikh, Sriraksha Srinath, Vani Rajashekaraiah

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引用次数: 0

摘要

背景:氧化应激(OS)引起红细胞的有害变化，导致血液溶解和巨噬细胞介导的清除循环。抗氧化剂已被证明可以保护红细胞免受体外诱导的OS。羟基苯甲酸(HBA)是一种酚类化合物，具有清除自由基的特性。因此，本研究旨在评估HBA对OS红细胞的作用。材料与方法:取雄性Wistar大鼠血液中分离的红细胞分为对照组和实验组，分别为2,2′-偶氮双(2-脒基丙烷)二盐酸(AAPH)、HBA10 (10 mM HBA)、HBA30 (30 mM HBA)组和AAPH与HBA联合使用组。测定OS指标和抗氧化水平。采用GraphPad Prism 8软件进行统计分析。结果:与对照组相比，HBA组和AAPH + HBA组血红蛋白降低。与对照组相比，HBA10中偶联二烯增加。硫代巴比妥酸活性物质(TBARSs)在AAPH + HBA10中升高。与AAPH相比，AAPH + HBA10组超氧化物歧化酶活性升高。与对照组相比，HBA10过氧化氢酶水平升高。结论:对羟基苯甲酸有利于清除AAPH产生的自由基，体现在溶血、巯基、TBARS和抗氧化酶的结果中。

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Effects of p-Hydroxybenzoic Acid on Erythrocytes Exposed to Oxidative Stress

Background: Oxidative stress (OS) causes deleterious changes in erythrocytes leading to hemolysis and macrophage-mediated removal from the circulation. Antioxidants have proven to protect the erythrocytes from induced OS in vitro. Hydroxybenzoic acid (HBA), a phenolic compound, exhibits free radical scavenging properties. Therefore, this study was conducted to evaluate the role of HBA on erythrocytes subjected to OS. Materials and Methods: Erythrocytes isolated from the blood collected from male Wistar rats were grouped into controls and experimental groups—samples with 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), HBA10 (10 mM HBA), HBA30 (30 mM HBA), and samples with combination of AAPH and HBA. The markers of OS and antioxidant status were determined. Statistical analyses were performed using GraphPad Prism 8 Software. Results: Hemoglobin decreased in HBA and AAPH + HBA groups against their respective controls. Conjugate dienes increased in HBA10 against control. Thiobarbituric acid reactive substances (TBARSs) increased in AAPH + HBA10 against AAPH. Superoxide dismutase activity elevated in the AAPH + HBA10 group compared with AAPH. Catalase levels increased in HBA10 against control. Conclusion: p-Hydroxybenzoic acid has proved to be beneficial in scavenging the free radicals generated by AAPH as reflected in the results of hemolysis, sulfhydryls, and TBARS and antioxidant enzymes.

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来源期刊

Applied In Vitro Toxicology Health Professions-Medical Laboratory Technology

CiteScore

2.70

自引率

0.00%

发文量

期刊介绍： Applied In Vitro Toxicology is a peer-reviewed journal providing the latest research on the application of alternative in vitro testing methods for predicting adverse effects in the pharmaceutical, chemical, and personal care industries. This Journal aims to address important issues facing the various chemical industries, including regulatory requirements; the reduction, refinement, and replacement of animal testing; new screening methods; evaluation of new cell and tissue models; and the most appropriate methods for assessing safety and satisfying regulatory demands. The Journal also delivers the latest views and opinions of developers of new models, end users of the models, academic laboratories that are inventing new tools, and regulatory agencies in the United States, Europe, Latin America, Australia and Asia. Applied In Vitro Toxicology is the journal that scientists involved with hazard identification and risk assessment will read to understand how new and existing in vitro methods are applied, and the questions for which these models provide answers. Applied In Vitro Toxicology coverage includes: -Applied in vitro toxicology industry standards -New technologies developed for applied in vitro toxicology -Data acquisition, cleaning, distribution, and best practices -Data protection, privacy, and policy -Business interests from research to product -The changing role of in vitro toxicology -Visualization and design principles of applied in vitro toxicology infrastructures -Physical interfaces and robotics -Opportunities around applied in vitro toxicology