Journal of Tropical Pathology最新文献

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PENGADAAN TANAH PADA KAWASAN HUTAN BAGI PEMBANGUNAN UNTUK KEPENTINGAN UMUM DI SEKTOR MIGAS 警告称,世界已恢复增长,用于移民部门的公共目的
Journal of Tropical Pathology Pub Date : 2020-11-30 DOI: 10.28946/RPT.V9I2.921
Firman Muntaqo, Sri Turatmiyah, Bagoes Mahendra Jaya, Machdum Satria
{"title":"PENGADAAN TANAH PADA KAWASAN HUTAN BAGI PEMBANGUNAN UNTUK KEPENTINGAN UMUM DI SEKTOR MIGAS","authors":"Firman Muntaqo, Sri Turatmiyah, Bagoes Mahendra Jaya, Machdum Satria","doi":"10.28946/RPT.V9I2.921","DOIUrl":"https://doi.org/10.28946/RPT.V9I2.921","url":null,"abstract":"Pembangunan untuk kepentingan umum di bidang minyak dan gas bumi, adalah prioritas pembangunan nasional  berbatas waktu. Berbagai kendala dihadapi dalam proses pengadaan tanahnya, terlebih jika tanah yang diperlukan berada di kawasan hutan. Penelitian menyimpulkan, kendala pengadaan tanah di kawasan hutan adalah akibat perundang-undangan di bidang kehutanan bersifat sektoral, penggunaan tanah dengan konsep yang berbeda sebagai hutan, yang tidak berdasarkan Undang-Undang Pokok Agraria, mengakibatkan terjadinya tumpang tindih pengaturan terhadap objek yang sama (tanah), tidak sinkron dan tidak harmonis dengan Undang-Undang Pokok Agraria. Untuk mengatasi dan mengeliminir berbagai kendala tersebut, penting dan mendesak dilakukan sinkronisasi dan harmonisasi Undang-Undangan Kehutanan dengan Undang-Undang Pokok Agraria, sebagai dasar pengadaan tanah bagi pembangunan untuk kepentingan umum di bidang minyak dan gas bumi di kawasan hutan. Dimasa mendatang, pemanfaatan tanah sebagai hutan oleh Kementrian Kehutanan dan Lingkungan Hidup seharusnya didasarkan pada Hak Pengelolaan Publik yang terdaftar, bersertifikat, yang secara administratif dikoordinir oleh Kementrian Agraria dan Tata Ruang.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":"9 1","pages":"71-84"},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42324227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
KONSEP KEABSAHAN KONTRAK ELEKTRONIK BERDASARKAN HUKUM NASIONAL DAN UNCITRAL MODEL LAW ON ELECTRONIC COMMERCE TAHUN 1996: STUDI PERBANDINGAN HUKUM DAN IMPILKASINYA DALAM HUKUM PERLINDUNGAN KONSUMEN 电子合同消费提供了国家和非国家电子商务示范法1996:人类消费及其在人类消费中的含义研究
Journal of Tropical Pathology Pub Date : 2020-11-30 DOI: 10.28946/RPT.V9I2.419
M. Alvi Syahrin
{"title":"KONSEP KEABSAHAN KONTRAK ELEKTRONIK BERDASARKAN HUKUM NASIONAL DAN UNCITRAL MODEL LAW ON ELECTRONIC COMMERCE TAHUN 1996: STUDI PERBANDINGAN HUKUM DAN IMPILKASINYA DALAM HUKUM PERLINDUNGAN KONSUMEN","authors":"M. Alvi Syahrin","doi":"10.28946/RPT.V9I2.419","DOIUrl":"https://doi.org/10.28946/RPT.V9I2.419","url":null,"abstract":"Pengaturan keabsahan kontrak elektronik di Indonesia diatur dalam berbagai regulasi. Namun praktiknya, perbedaan tersebut berpotensi menimbulkan konflik dan ketidakpastian hukum di antara para pihak. Rumusan masalah yang diteliti adalah bagaimana implikasi hukum dari perbedaan pengaturan keabsahan kontrak elektronik berdasarkan hukum nasional dan UNCITRAL Model Law on Electronic Commerce Tahun 1996 dalam hukum perlindungan konsumen. Metode penelitian yang digunakan adalah penelitian hukum normatif yang membahas doktrin atau asas dalam ilmu hukum yang bertujuan untuk menemukan kaidah hukum positif. Implikasi hukum perbedaan pengaturan kontrak elektronik dalam pengaturan keabsahan kontrak elektronik berdasarkan hukum nasional dan UNCITRAL Model Law On Electronic Commerce Tahun 1996 telah menimbulkan implikasi hukum bagi perlindungan konsumen, yaitu: privasi, klausula baku, otensitas subjek hukum, validitas subjek hukum, objek e-commerce, dan tanggung jawab para pihak.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":"9 1","pages":"105-122"},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42751762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
KEPASTIAN HUKUM PELAKSANAAN KONTRAK KONSTRUKSI 施工合同
Journal of Tropical Pathology Pub Date : 2020-11-30 DOI: 10.28946/RPT.V9I2.630
Rio Christiawan
{"title":"KEPASTIAN HUKUM PELAKSANAAN KONTRAK KONSTRUKSI","authors":"Rio Christiawan","doi":"10.28946/RPT.V9I2.630","DOIUrl":"https://doi.org/10.28946/RPT.V9I2.630","url":null,"abstract":"Abstrak: Pembangunan infrastruktur menjadi prioritas pada pemerintahan Presiden Joko Widodo sehingga dalam hal ini pekerjaan konstruksi menjadi sangat penting untuk menunjang misi pembangunan Presiden. Mengingat pentingnya pelaksanaan pekerjaan konstruksi guna pembangunan maka secara hukum harus dibarengi dengan adanya kepastian hukum terkait pelaksanaan kontrak konstruksi yang dibuat oleh para pihak. Persoalannya dalam hal ini kontrak yang pada awalnya dibuat untuk mewujudkan kepastian hukum kini justru dengan instrument hukum yang ada termasuk Undang-Undang Nomor 2 Tahun 2017 tentang Jasa Konstruksi justru dipandang menimbulkan ketidakpastian hukum karena kontrak yang seharusnya masuk dalam domain hukum perdata menjadi dimungkinkan masuk dalam ranah hukum pidana. Banyaknya pelaku usaha yang menghadapi persoalan pidana terkait dengan pelaksanaan kontrak konstruksi justru dipandang sebagai hambatan pembangunan dan investasi yang disebabkan karena ketidakpastian hukum. Kata kunci: Kepastian Hukum; Kontrak; Pekerjaan Konstruksi; Hambatan Pembangunan","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":"9 1","pages":"85-94"},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41970658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seroprevalence of anti-TRYPANOSOMA CRUZI and anti-TOXOPLASMA GONDII antibodies in possible and potential organ donors in the south of Rio Grande do Sul state, Brazil 巴西南巴西大德州南部可能和潜在器官供体中抗克氏锥虫和抗弓形虫抗体的血清阳性率
Journal of Tropical Pathology Pub Date : 2020-10-19 DOI: 10.5216/rpt.v49i3.63938
Ana Paula da Paz Grala, Tanise Freitas Bianchi, Italo Ferreira de Leon, Mayara Guellamann da Cunha Espinelli Greco, Fabio Raphael Pascoti Bruhn, Nivia Celoi Ferreira Barargan, Viviani Aspirot Mendonça, Marcos Marreiro Villela
{"title":"Seroprevalence of anti-TRYPANOSOMA CRUZI and anti-TOXOPLASMA GONDII antibodies in possible and potential organ donors in the south of Rio Grande do Sul state, Brazil","authors":"Ana Paula da Paz Grala, Tanise Freitas Bianchi, Italo Ferreira de Leon, Mayara Guellamann da Cunha Espinelli Greco, Fabio Raphael Pascoti Bruhn, Nivia Celoi Ferreira Barargan, Viviani Aspirot Mendonça, Marcos Marreiro Villela","doi":"10.5216/rpt.v49i3.63938","DOIUrl":"https://doi.org/10.5216/rpt.v49i3.63938","url":null,"abstract":"Serological profiles are important in cases of solid organ donation where serological findings, such as antibodies against Trypanosoma cruzi and toxoplasmosis, may interfere in organ donation by increasing morbidity and mortality. This study aimed to outline seroprevalence of anti-Trypanosoma cruzi and anti-Toxoplasma gondii antibodies in possible and potential organ donors in the south of Rio Grande do Sul (RS) State, Brazil. A cross-sectional quantitative and retrospective epidemiological study was carried out, based on secondary data. Data on serology were extracted from medical records found in the Sistema de Gestão Hospitalar (SIGH), a hospital management system used by a university hospital in the south of RS, Brazil. Sociodemographic variables, such as age, sex and race, as well as clinical variables, such as titration of IgM and IgG anti-T. cruzi and anti–T. gondii antibodies, were analyzed. Medical records were found to be poorly filled in, since 67.6% (506 records) could not be used due to lack of information. Seroprevalence of T. cruzi was 6.8% (5 cases), mostly in white males. Regarding seroprevalence of T. gondii, 76.2% (64 donors) were serologicaly positive, 10.9% of which were IgM positive. This is the first study on anti-T. cruzi and anti-T. gondii antibodies in organ donors in RS, Brazil and should be furthered since there may be consequences regarding organ uptake and donation.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43318591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human african trypanosomiasis: current standing and challenges 非洲人类锥虫病:现状和挑战
Journal of Tropical Pathology Pub Date : 2020-10-19 DOI: 10.5216/rpt.v49i3.62857
Isabel Theresa Holanda-Freitas, Marli do Cupertino, Elizária C. dos Santos, Lisa Oliveira, M. Geller, R. Siqueira-Batista
{"title":"Human african trypanosomiasis: current standing and challenges","authors":"Isabel Theresa Holanda-Freitas, Marli do Cupertino, Elizária C. dos Santos, Lisa Oliveira, M. Geller, R. Siqueira-Batista","doi":"10.5216/rpt.v49i3.62857","DOIUrl":"https://doi.org/10.5216/rpt.v49i3.62857","url":null,"abstract":"Human African trypanosomiasis (HAT) caused by the protozoan Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense, and transmitted by the tsetse fly (genus Glossina), affects 36 Sub-Saharan African countries with considerable public health impact.  Despite approximately 15,000 infected individuals and 70 million at risk, in recent years the World Health Organization has mentioned removal of HAT from the list of Neglected Tropical Diseases by 2020, due to the decrease in cases over the last two decades. When untreated, the disease presents high lethality rates and the available treatments are complicated to administer, highly toxic, and do not guarantee cure, especially in the advanced stages of the disease. Further, there is no prospect for vaccine development in the near future. The present review compiles information on the history of the clinical aspects of HAT, as well as its epidemiology, diagnosis, therapy, and prophylaxis, as well as updating information on the current panorama and perspectives regarding the disease.KEY WORDS: African Trypanosomiasis; neglected diseases; Trypanosoma brucei.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82961433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Occurrence of parasites in salads in restaurants in Aparecida de Goiânia, Goiás, Brazil 巴西阿帕雷西达戈伊<e:2>尼亚Goiás市餐馆沙拉中寄生虫的发生情况
Journal of Tropical Pathology Pub Date : 2020-09-30 DOI: 10.5216/rpt.v49i3.62681
Lucas Perim, N. Custodio, V. D. C. V. Lima, Jaqueline Ataíde Silva Lima da Igreja, Daniella de Sousa Mendes Moreira Alves, Heloísa Ribeiro Storchilo, Antonio Roberto Gomes Junior, A. M. Castro, W. F. D. Costa, H. Rezende
{"title":"Occurrence of parasites in salads in restaurants in Aparecida de Goiânia, Goiás, Brazil","authors":"Lucas Perim, N. Custodio, V. D. C. V. Lima, Jaqueline Ataíde Silva Lima da Igreja, Daniella de Sousa Mendes Moreira Alves, Heloísa Ribeiro Storchilo, Antonio Roberto Gomes Junior, A. M. Castro, W. F. D. Costa, H. Rezende","doi":"10.5216/rpt.v49i3.62681","DOIUrl":"https://doi.org/10.5216/rpt.v49i3.62681","url":null,"abstract":"Parasitic diseases are the most widespread diseases in the world. They are transmited via contaminated water or food. Considering that the daily consumption of vegetables is estimated at 142g per person, the purpose of this study was to evaluate the occurrence of parasites in salads available for consumption in restaurants in Aparecida de Goiânia, Goiás State. Salad samples were collected from the restaurants and parasitological analysis was performed using the Willis, Hoffman, Faust and Ziehl Neelsen techniques as well as cultures for the isolation of free-living amoebae. 51 samples were analyzed, 16 (31.4%) were positive. The parasites detected were: Acanthamoeba spp. in 12 (23.5%); free-living larvae, Schistosoma mansoni and Entamoeba coli in 1 (2.0%); Endolimax nana in 2 (3.9%). The PCR technique determined that 17.6% of the samples presented Toxoplasma gondii DNA. These techniques evidenced that the salad samples presented parasite contamination not only in the restaurants with the lowest price per Kg, but also in the most expensive ones. Therefore, in addition to effective sanitary surveillance, prophylactic measures are necessary regarding suppliers, handlers and restaurant owners to prevent the spread of these and other parasites.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43709905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Parasite detection in sand from bays on the north coast of São Paulo state, Brazil 巴西<s:1>圣保罗州北海岸海湾沙子中的寄生虫检测
Journal of Tropical Pathology Pub Date : 2020-09-30 DOI: 10.5216/rpt.v49i3.63783
Eliézer Lucas Pires Ramos, C. Gómez-Hérnandez, Lucas Gonçalves Queiroz, Renata Gregorio Franco Moura, Nathalia Pires Nogueira, Gabriela Lícia Santos Ferreira, Karine Rezende-Oliveira
{"title":"Parasite detection in sand from bays on the north coast of São Paulo state, Brazil","authors":"Eliézer Lucas Pires Ramos, C. Gómez-Hérnandez, Lucas Gonçalves Queiroz, Renata Gregorio Franco Moura, Nathalia Pires Nogueira, Gabriela Lícia Santos Ferreira, Karine Rezende-Oliveira","doi":"10.5216/rpt.v49i3.63783","DOIUrl":"https://doi.org/10.5216/rpt.v49i3.63783","url":null,"abstract":"Soil contamination by protozoan parasites and soil-transmitted helminths (STH) is common in beach sand due to a number of factors such as pets, pluvial water, garbage, etc. These pathogens may cause many diseases in humans and animals and become a public health problem. Thus, systematic evaluation and inspection are necessary to develop control strategies regarding public contamination. For this purpose, our aims were to evaluate the parasitic profile of sandy soils on an urban beach and an untouched beach and correlate this with environmental and seasonal characteristics in Ubatuba, Brazil, in two seasons (winter and summer). 132 soilsamples were collected for parasite analysis utilizing Rugai’s method and the sedimentation adapted method. Our results showed positivity in 62% of the samples for at least one parasite in the urban beach and no parasitic structures on the untouched beach. The positivity was higher in summer (85%) than in winter (51.7%). All samples were positive for both, helminths and protozoa. Seasonal influence was noted regarding the presence of STH while for protozoa this influence was not observed. The parasitic structures most found were larvae of hookworms (35%) and Toxocara eggs (31.7%). We also noted the presence of Strongyloides sp, Ascarislumbricoides, coccidia, Dipylidium caninum, Entamoeba hystolitica/dispar, Endolimax nana, Entamoeba coli, Giardia sp, Toxascaris leonina, Trichuris sp and Dibothricephalus latus. There was a positive correlation between temperature and the presence of STH in the sand samples. Most of the collection sites on the urban beach presented dogs or canine traces and garbage in both seasons. There was an association between the presence of dogs or their traces and parasitic structures. In conclusion, seasonality, urbanization and the presence of pets on beaches can potentially favor environmental contamination by parasites increasing the risk oftransmission of zoonotic and parasitic diseases.KEY WORDS: Soil-transmitted helminths; protozoa; environmental contamination; sand; beaches.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90331098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Epidemiology and predictors of occurrence of SCHISTOSOMA MANSONI infection in a low-endemicity area in northeast Brazil 巴西东北部低流行区曼氏血吸虫感染流行病学及预测因素
Journal of Tropical Pathology Pub Date : 2020-09-30 DOI: 10.5216/rpt.v49i3.62455
M. S. Sousa, M. Pinheiro, A. Júnior, José Damião da Silva Filho, F. Bezerra
{"title":"Epidemiology and predictors of occurrence of SCHISTOSOMA MANSONI infection in a low-endemicity area in northeast Brazil","authors":"M. S. Sousa, M. Pinheiro, A. Júnior, José Damião da Silva Filho, F. Bezerra","doi":"10.5216/rpt.v49i3.62455","DOIUrl":"https://doi.org/10.5216/rpt.v49i3.62455","url":null,"abstract":"In Latin America 96% of the cases of schistosomiasis occur in Brazil in low-socioeconomic status populations. The epidemiological characteristics and occurrence predictors of Schistosoma mansoni infection were determined in the Bananeiras community, located in Capistrano, a town in Ceará state, Brazil. Sanitary, environmental, socioeconomic, and behavioral data were collected using a semi-structured questionnaire. An investigation to assess S. mansoni infection was conducted using the Kato-Katz and Point-of-Care Circulating Cathodic Antigen (POC-CCA) methods. From the 258 subjects were analyzed, 54.3% (n=140) were women, median age 30 years. Thirty-three (12.8%) individuals were positive by either eggand/or CCA-positivity. The highest positivity rate was found in the 30-39 year old group. There was no piped water supply, sewage network or municipal refuse collection service. Most individuals were illiterate or had not finished elementary school (66.3%). About 29.1% of the families had a monthly income below one Brazilian minimum wage and 91.1% reported contact with natural water sources. We found an association between infection and age group of 20-40 years, illiteracy, household with 7 inhabitants or more, household with up to 3 rooms and an outhouse. Contrarily, being 40 years old or older and household with up to 6 inhabitants were not risk factors. Schistosomiasis remains a public health problem in this municipality, evidencing a strong association with low socioeconomic conditions and high vulnerability. These findings reinforce the importance of identifying the factors associated with the infection for more effective guidance in actions in control programs targeting schistosomiasis prevention and control.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42800910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accuracy in clinical examinations for the diagnosis of vulvovaginitis by CANDIDA SPP. and IN VITRO susceptibility to the main antifungals 念珠菌spp诊断外阴阴道炎临床检查的准确性及对主要抗真菌药物的体外敏感性
Journal of Tropical Pathology Pub Date : 2020-09-29 DOI: 10.5216/rpt.v49i3.64233
Andressa Santana Santos, A. Zara, F. Ataídes, Elisangela Pimentel da Silva, Vivianny Aparecida Queiroz Freitas, C. R. Costa, Thaísa C Silva, Maria R R Silva
{"title":"Accuracy in clinical examinations for the diagnosis of vulvovaginitis by CANDIDA SPP. and IN VITRO susceptibility to the main antifungals","authors":"Andressa Santana Santos, A. Zara, F. Ataídes, Elisangela Pimentel da Silva, Vivianny Aparecida Queiroz Freitas, C. R. Costa, Thaísa C Silva, Maria R R Silva","doi":"10.5216/rpt.v49i3.64233","DOIUrl":"https://doi.org/10.5216/rpt.v49i3.64233","url":null,"abstract":"Vulvovaginal candidiasis (VVC) is a common infection. This work aims to determine the positive predictive value (PPV) of the clinical diagnosis of VVC and to characterize Candida species isolated from the vaginal mucosa. This cross-sectional study was conducted from February 2016 to February 2017 at the Gynecology and Obstetrics Outpatient Clinic of the Hospital das Clínicas, in Goiânia, Goiás State, Brazil. The study included samples of vaginal secretion from 55 women who complained of vaginal discharge and itching as their main symptoms. The PPV of the clinical diagnosis of VVC was estimated in comparison to the laboratory culture method. The phenotypic methods and molecular tests were performed to identify Candida spp. In vitro susceptibility of Candida spp. isolates to fluconazole, itraconazole, clotrimazole, nystatin, and amphotericin B was determined using the broth microdilution assay. Yeast growth using the enzymes protease, phospholipase, and hemolysin was carried out in media containing respectively bovine albumin, egg yolk, and sheep erythrocytes. A PPV of 61.8% (34/55) was determined. Among the 55 vulvovaginal samples collected, we identified 36 isolates in which C. albicans was the most common species. High resistance to fluconazole and low minimal inhibitory concentration (MIC) values for clotrimazole, nystatin and amphotericin B were observed. All isolates were proteinase and hemolysin producers, while seven strains were phospholipase negative. The clinical diagnosis of VVC presented a moderate PPV, which meant that cultures had to be conducted in the laboratory to confirm infection. The high resistance to fluconazole and itraconazole indicated the importance of the in vitro susceptibility test.","PeriodicalId":36789,"journal":{"name":"Journal of Tropical Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45969230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MULTICENTER STUDY OF CANDIDA SPECIES IN ORAL MUCOSA OF DIFFERENT PATIENTS: ANALYSIS OF 711 STRAINS AND LITERATURE REVIEW 口腔黏膜念珠菌711株分析及文献复习
Journal of Tropical Pathology Pub Date : 2020-07-19 DOI: 10.5216/rpt.v49i2.63481
Rennan Luiz Oliveira Dos Santos, L. Ruiz, M. Auler, J. K. Chavasco, D. Moreira, R. T. Ramos, B. R. Lara, C. R. Paula
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