Recycling最新文献

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Quality-Driven Allocation Method to Promote the Circular Economy for Plastic Components in the Automotive Industry 促进汽车行业塑料部件循环经济的质量驱动分配法
IF 4.6
Recycling Pub Date : 2024-08-09 DOI: 10.3390/recycling9040067
Ilka Pfisterer, R. Rinberg, Lothar Kroll, Niels Modler
{"title":"Quality-Driven Allocation Method to Promote the Circular Economy for Plastic Components in the Automotive Industry","authors":"Ilka Pfisterer, R. Rinberg, Lothar Kroll, Niels Modler","doi":"10.3390/recycling9040067","DOIUrl":"https://doi.org/10.3390/recycling9040067","url":null,"abstract":"In recent years, the development of a circular economy of plastic products in the automotive industry has been pursued by original equipment manufacturers (OEMs) not only due to strategic premises by the European Commission but also due to an increasing demand by customers. To achieve a circular economy, high-quality recyclates are needed. However, in the current situation, there is a discrepancy between the low-quality recyclate that is available on the market and the high-quality recyclate that is demanded by manufacturers. To increase the quality of recyclate on the market, a standardized process to reward a ‘design-for-recycling’ approach at the product development stage is needed. This paper proposes an allocation method that takes into account material compositions and common recycling processes and incentivizes the preservation of high-quality grades of recyclate based on grade purity.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141921968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Silicon Kerf Recovery via Acid Leaching Followed by Melting at Elevated Temperatures 通过酸性浸出并在高温下熔化回收硅片层
IF 4.6
Recycling Pub Date : 2024-08-08 DOI: 10.3390/recycling9040066
Tinotenda Mubaiwa, Askh Garshol, A. Azarov, J. Safarian
{"title":"Silicon Kerf Recovery via Acid Leaching Followed by Melting at Elevated Temperatures","authors":"Tinotenda Mubaiwa, Askh Garshol, A. Azarov, J. Safarian","doi":"10.3390/recycling9040066","DOIUrl":"https://doi.org/10.3390/recycling9040066","url":null,"abstract":"The aim of this work was to study the purification of silicon kerf loss waste (KLW) by a combination of single-acid leaching followed by inductive melting at high temperatures with an addition of fluidized bed reactor (FBR) silicon granules. The KLW indicated an average particle size (D50) of approximately 1.6 µm, and a BET surface area of 30.4 m2/g. Acid leaching by 1 M HCl indicated significant removal of impurities such as Ni (77%), Fe (91%) and P (75%). The combined two-stage treatment resulted in significant removal of the major impurities: Al (78%), Ni (79%), Ca (85%), P (92%) and Fe (99%). The general material loss during melting decreased with an increasing amount of FBR silicon granules which aided in the melting process and indicated better melting. It was observed that the melting behavior of the samples improved as the temperature increased, with complete melting being observed throughout the crucibles at the highest temperature (1800 °C) used, even without any additives. At lower temperatures (1600 °C–1700 °C) and lower FBR-Si (<30 wt.%) additions, the melting was incomplete, with patches of molten silicon and a lot of surface oxidation as confirmed by both visual observation and electron microscopy. In addition, it was indicated that more reactive and volatile elements (Ga, Mg and P) compared to silicon are partially removed in the melting process (51–87%), while the less reactive elements end up in the final silicon melt. It was concluded that if optimized, the combined treatment of single-acid leaching and inductive melting with the addition of granular FBR silicon has great potential for the recycling of KLW to solar cells and similar applications. Moreover, the application of higher melting temperatures is accompanied by a higher silicon yield of the process, and the involved mechanisms are presented.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141926915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Investigation into Sustainable Solutions: Utilizing Hydrated Lime Derived from Oyster Shells as an Eco-Friendly Alternative for Semiconductor Wastewater Treatment 对可持续解决方案的研究:利用牡蛎壳中提取的水合石灰作为半导体废水处理的生态友好替代品
IF 4.6
Recycling Pub Date : 2024-07-26 DOI: 10.3390/recycling9040061
Hye-Jin Lee, Sang-Eun Lee, Seokhwi Kim
{"title":"An Investigation into Sustainable Solutions: Utilizing Hydrated Lime Derived from Oyster Shells as an Eco-Friendly Alternative for Semiconductor Wastewater Treatment","authors":"Hye-Jin Lee, Sang-Eun Lee, Seokhwi Kim","doi":"10.3390/recycling9040061","DOIUrl":"https://doi.org/10.3390/recycling9040061","url":null,"abstract":"Due to its acidic nature and high fluoride concentration, hydrated lime (Ca(OH)2) is commonly used for neutralization and fluoride control in semiconductor wastewater treatment. This study investigated the efficacy of treating high fluoride-containing wastewater using hydrated lime derived from oyster shells as an alternative to limestone. Overall, the characteristics of removing pollutants in acidic wastewater using shell-based hydrated lime showed similar patterns to hydrated lime from limestone. The treatment efficiency was 50% or less under theoretical Ca/F molar ratio (=0.5) conditions for the formation of fluorite (CaF2), while the fluorine removal rate reached 99% under somewhat higher Ca/F conditions due to the influence of ionic components in the wastewater. Interestingly, chloride content did not increase even in the initial reaction stages, in contrast to our concerns about oyster shells generally containing salt to a certain extent due to their growth in seawater; instead, the chloride concentration decreased over time, similar to nitrate (NO3−). In controlling fluoride in wastewater, surpassing the theoretical Ca/F molar ratio, particularly considering the presence of other anionic species such as SO4²− and PO4³−, the optimal Ca/F ratio for fluoride removal was found to be 1.59. This value is approximately 16% lower than the calculated value (Ca/F = 1.85) when accounting for other anions. X-ray diffraction results confirmed the presence of CaSO4, Ca3(PO4)2, and CaF2 in the precipitate recovered after the reaction, indicating the effective removal of ionic contaminants. This observation suggests that oyster shell-derived hydrated lime could serve as a viable calcium resource for treating acidic wastewater and represents a potential alternative to traditional limestone-based methods.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141799516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Environmental and Economic Forecast of the Widespread Use of Anaerobic Digestion Techniques 广泛使用厌氧消化技术的环境和经济预测
IF 4.6
Recycling Pub Date : 2024-07-26 DOI: 10.3390/recycling9040062
A. Kiselev, E. Magaril, Ramona Giurea
{"title":"Environmental and Economic Forecast of the Widespread Use of Anaerobic Digestion Techniques","authors":"A. Kiselev, E. Magaril, Ramona Giurea","doi":"10.3390/recycling9040062","DOIUrl":"https://doi.org/10.3390/recycling9040062","url":null,"abstract":"The concept of the circular economy represents the most relevant mainstream approach to reducing the negative environmental impact of waste. Anaerobic digestion has proved to be one of the leading and widely adopted techniques for sewage sludge treatment under the principles of the circular economy. The purpose of this study is to forecast environmental and economic indicators through modeling the extensive utilization of biogas technologies with a case study of an administrative territorial unit. The proposed methodological framework involves the use of averaged specific indicators and is based on the relationship between inhabitants, waste generation rates, biogas yield, greenhouse gas emission mitigation and biogas energy potential. The widespread use of anaerobic digestion techniques according to the proposed methodology in the instant scenario will ensure the biogas yield of 10 million Nm3 within the considered administrative territory unit with a population of 4.2 million P.E., which ultimately can be expressed in electricity and thermal generation potential of 20.8 and 24.8 million kWh*y, respectively, annual greenhouse gas elimination of 119.6 thousand tons of CO2 equivalent and capital investment attraction of EUR 65.18 million. Furthermore, all sewage sludge will be subjected to disinfection and stabilization procedures to ensure its safe utilization. The findings of this study offer an opportunity for a wide range of stakeholders to assess the environmental and economic benefits of the widespread adoption of biogas technologies. The developed methodology can be utilized to inform management decisions through the use of the instant and scenario forecasts.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141801903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Concentration of Silver from Recycling of Fine Powder of Wasted Videogame Printed Circuit Boards through Reverse Froth Flotation and Magnetic Separation Processes 通过反浮选和磁分离工艺从废弃视频游戏印刷电路板细粉回收中提炼银
IF 4.6
Recycling Pub Date : 2024-07-22 DOI: 10.3390/recycling9040060
Rubén Flores-Campos, Rosa Hilda Estrada-Ruiz, Mario Rodríguez-Reyes, Diego Martínez-Carrillo, A. Martínez-Luévanos
{"title":"Concentration of Silver from Recycling of Fine Powder of Wasted Videogame Printed Circuit Boards through Reverse Froth Flotation and Magnetic Separation Processes","authors":"Rubén Flores-Campos, Rosa Hilda Estrada-Ruiz, Mario Rodríguez-Reyes, Diego Martínez-Carrillo, A. Martínez-Luévanos","doi":"10.3390/recycling9040060","DOIUrl":"https://doi.org/10.3390/recycling9040060","url":null,"abstract":"Electronic waste stream grows day by day; printed circuit boards are a kind of solid waste that accounts for 6% of electronic waste. When these are discarded, they can cause soil, water, and air contamination; however, if recycled, these can be considered as a secondary source of metals. Physical comminution of printed circuit boards generates particles with sizes smaller than 250 µm, which are typically not included in the recycling process because they are considered as dust and unvaluable; nevertheless, precious and base metals can be found in these particles. The concentration of metals like silver, among others, from these particles can be achieved by reverse froth flotation in a flotation column followed by magnetic separation of the tails products. A mass balance of the flotation column feed, concentrate, and tails indicates that using a pulp modified with 5 ppm methyl isobutyl carbinol plus 5 g/ton oleic acid (both biodegradable reagents), the concentration of the products improved, resulting in recoveries of 86.13 and 13.87% in the concentrate and tails zones, respectively, with a grade of 74.4% in the tails flow. Magnetic separation of the tails product increases slightly the concentration of silver, reaching a silver grade of 74.5%, a recovery amount similar to those obtained employing complex and environmentally unfriendly processes.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141814954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A New Recycling Method through Mushroom Cultivation Using Food Waste: Optimization of Mushroom Bed Medium Using Food Waste and Agricultural Use of Spent Mushroom Substrates 利用厨余栽培蘑菇的新循环方法:利用厨余优化蘑菇床培养基和废蘑菇基质的农业利用
IF 4.6
Recycling Pub Date : 2024-07-15 DOI: 10.3390/recycling9040058
Babla Shingha Barua, Ami Nigaki, Ryota Kataoka
{"title":"A New Recycling Method through Mushroom Cultivation Using Food Waste: Optimization of Mushroom Bed Medium Using Food Waste and Agricultural Use of Spent Mushroom Substrates","authors":"Babla Shingha Barua, Ami Nigaki, Ryota Kataoka","doi":"10.3390/recycling9040058","DOIUrl":"https://doi.org/10.3390/recycling9040058","url":null,"abstract":"Food waste is generated in large amounts locally and globally, and requires expenditure for disposal. However, it has high nutritional value and almost no toxic components. Therefore, it can be returned to mushroom mediums for further use, leading to food waste circulation. Though disposing of spent mushroom substrate (SMS) after harvesting is an additional problem, there have been increased efforts to compost it and apply it to the soil for growing vegetables. This study, therefore, aimed to optimize (1) mushroom spawn production with rice hull, (2) mushroom substrates using food waste to accelerate food waste recycling, and (3) the utilization of SMS as an organic fertilizer. An optimal substrate composition and high yield were obtained at 120–140 g of food waste per bag among substrates from Pleorutus ostreatus and Pleorutus citrinopileatus; therefore, using a high ratio of food waste in the mushroom mediums was achieved. On the other hand, the SMS of P. citrinopileatus demonstrated higher plant biomass growth, at 36 g, than that of P. ostreatus, at 21.2 g, in a treatment using SMS + okara. The present discovery is that people may be encouraged to be mindful of food loss by the delivery of mushrooms and plants grown from agro/food waste to the dining table, and this circular system may therefore be used as a key resource in mushroom and plant cultivation and to achieve a zero-emission cycle.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141646198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent Developments in Technology for Sorting Plastic for Recycling: The Emergence of Artificial Intelligence and the Rise of the Robots 塑料回收分类技术的最新发展:人工智能的出现和机器人的崛起
IF 4.6
Recycling Pub Date : 2024-07-15 DOI: 10.3390/recycling9040059
Cesar Lubongo, Mohammed A. A. Bin Daej, P. Alexandridis
{"title":"Recent Developments in Technology for Sorting Plastic for Recycling: The Emergence of Artificial Intelligence and the Rise of the Robots","authors":"Cesar Lubongo, Mohammed A. A. Bin Daej, P. Alexandridis","doi":"10.3390/recycling9040059","DOIUrl":"https://doi.org/10.3390/recycling9040059","url":null,"abstract":"Plastics recycling is an important component of the circular economy. In mechanical recycling, the recovery of high-quality plastics for subsequent reprocessing requires plastic waste to be first sorted by type, color, and size. In chemical recycling, certain types of plastics should be removed first as they negatively affect the process. Such sortation of plastic objects at Materials Recovery Facilities (MRFs) relies increasingly on automated technology. Critical for any sorting is the proper identification of the plastic type. Spectroscopy is used to this end, increasingly augmented by machine learning (ML) and artificial intelligence (AI). Recent developments in the application of ML/AI in plastics recycling are highlighted here, and the state of the art in the identification and sortation of plastic is presented. Commercial equipment for sorting plastic recyclables is identified from a survey of publicly available information. Automated sorting equipment, ML/AI-based sorters, and robotic sorters currently available on the market are evaluated regarding their sensors, capability to sort certain types of plastics, primary application, throughput, and accuracy. This information reflects the rapid progress achieved in sorting plastics. However, the sortation of film, dark plastics, and plastics comprising multiple types of polymers remains challenging. Improvements and/or new solutions in the automated sorting of plastics are forthcoming.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141646197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of the Effectiveness of Barrier Layers to Inhibit Mutagenic Effects of Recycled LDPE Films, Using a Miniaturized Ames Test and GC-MS Analysis 利用微型阿姆斯试验和气相色谱-质谱分析法研究阻隔层抑制回收低密度聚乙烯薄膜诱变作用的效果
IF 4.6
Recycling Pub Date : 2024-07-15 DOI: 10.3390/recycling9040057
Lukas Prielinger, S. Bandyopadhyay, Eva Ortner, Martin Novak, Tanja Radusin, Steffen Annfinsen, N. Sharmin, Bernhard Rainer, M. K. Pettersen
{"title":"Investigation of the Effectiveness of Barrier Layers to Inhibit Mutagenic Effects of Recycled LDPE Films, Using a Miniaturized Ames Test and GC-MS Analysis","authors":"Lukas Prielinger, S. Bandyopadhyay, Eva Ortner, Martin Novak, Tanja Radusin, Steffen Annfinsen, N. Sharmin, Bernhard Rainer, M. K. Pettersen","doi":"10.3390/recycling9040057","DOIUrl":"https://doi.org/10.3390/recycling9040057","url":null,"abstract":"To fulfil the European Green Deal targets and implement a circular economy, there is an urgent need to increase recycling rates of packaging materials. However, before recycled materials can be used in food contact applications, they must meet high safety standards. According to the European Food Safety Authority (EFSA), a worst-case scenario must be applied and unknown substances must be evaluated as being potentially genotoxic. The Ames test, which detects direct DNA-reactive effects, together with chromatographic analysis is very promising to complement risk assessment. This study aims to evaluate the effectiveness of functional barriers in ten different samples, including virgin and recycled LDPE foils. FT-IR analysis did not show major differences between virgin and recycled films. Light microscopy revealed differences in quality and an increased number of particles. GC-MS analysis detected and quantified 35 substances, including eight unknowns. Using a miniaturized version of the Ames test, four of ten samples tested positive in two individual migrates up to a dilution of 12.5%. All virgin LDPE materials tested negative; however, recycled material F showed an increased mutagenic activity, with an n-fold induction up to 28. Samples with functional barriers lowered migration and reduced mutagenicity. Nonetheless, further investigations are needed to identify possible sources of contamination.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141649373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Use of Nonmetallic Fraction Particles with the Double Purpose of Increasing the Mechanical Properties of Low-Density Polyethylene Composite and Reducing the Pollution Associated with the Recycling of Metals from E-Waste 使用非金属馏分微粒具有双重目的:提高低密度聚乙烯复合材料的机械性能和减少从电子垃圾中回收金属所造成的污染
IF 4.6
Recycling Pub Date : 2024-07-03 DOI: 10.3390/recycling9040056
R. Flores-Campos, R. Deaquino-Lara, Mario Rodríguez-Reyes, R. Martínez-Sánchez, Rosa Hilda Estrada-Ruiz
{"title":"The Use of Nonmetallic Fraction Particles with the Double Purpose of Increasing the Mechanical Properties of Low-Density Polyethylene Composite and Reducing the Pollution Associated with the Recycling of Metals from E-Waste","authors":"R. Flores-Campos, R. Deaquino-Lara, Mario Rodríguez-Reyes, R. Martínez-Sánchez, Rosa Hilda Estrada-Ruiz","doi":"10.3390/recycling9040056","DOIUrl":"https://doi.org/10.3390/recycling9040056","url":null,"abstract":"A restorative process, where the nonmetallic fraction from e-waste printed circuit boards is reused as a raw material for the conformation of a new polymer matrix composite with increased properties favoring its industrial applications, is proposed with a zero residues approach. Low density polyethylene pellets and nonmetallic fraction particles were mixed, and due to the generation of static electricity during the mixing process, the nonmetallic particles became attached to the polyethylene pellets; the blended material was fed into a screw extruder, producing filaments of the new composite. Mechanical properties increased as the particles content increased, presenting an ultimate tensile strength going from 20 for the raw low-density polyethylene to more than 60 MPa, and a yield strength that goes from 10 to 50 MPa on the composite with 6.0 wt.% particles. Also, the flammability of the composite improved, reducing its linear burning rate and increasing the time between detachment of two consecutive drops. Nonmetallic fraction particles were oriented in the extrusion direction and had a good adhesion with the polyethylene matrix. These composites can be employed for the production of prototypes using additive manufacture.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141680331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization Production of an Endo-β-1,4-Xylanase from Streptomyces thermocarboxydus Using Wheat Bran as Sole Carbon Source 以麦麸为唯一碳源优化热羧酸链霉菌内β-1,4-木聚糖酶的生产
IF 4.3
Recycling Pub Date : 2024-06-09 DOI: 10.3390/recycling9030050
Thi Ngoc Tran, C. Doan, Thi Kieu Loan Dinh, Thi Hai Ninh Duong, Thi Thuc Uyen Phan, Thi Thuy Loan Le, Trung Dung Tran, Pham Hung Quang Hoang, A. Nguyen, San-Lang Wang
{"title":"Optimization Production of an Endo-β-1,4-Xylanase from Streptomyces thermocarboxydus Using Wheat Bran as Sole Carbon Source","authors":"Thi Ngoc Tran, C. Doan, Thi Kieu Loan Dinh, Thi Hai Ninh Duong, Thi Thuc Uyen Phan, Thi Thuy Loan Le, Trung Dung Tran, Pham Hung Quang Hoang, A. Nguyen, San-Lang Wang","doi":"10.3390/recycling9030050","DOIUrl":"https://doi.org/10.3390/recycling9030050","url":null,"abstract":"Xylanases, key enzymes for hydrolyzing xylan, have diverse industrial applications. The bioprocessing of agricultural byproducts to produce xylanase through fermentation approaches is gaining importance due to its significant potential to reduce enzyme production costs. In this work, the productivity of Streptomyces thermocarboxydus TKU045 xylanase was enhanced through liquid fermentation employing wheat bran as the sole carbon source. The maximum xylanase activity (25.314 ± 1.635 U/mL) was obtained using the following optima factors: 2% (w/v) wheat bran, 1.4% (w/v) KNO3, an initial pH of 9.8, an incubation temperature of 37.3 °C, and an incubation time of 2.2 days. Xylanase (Xyn_TKU045) of 43 kDa molecular weight was isolated from the culture supernatant and was biochemically characterized. Analysis through liquid chromatography with tandem mass spectrometry revealed a maximum amino acid identity of 19% with an endo-1,4-β-xylanase produced by Streptomyces lividans. Xyn_TKU045 exhibited optimal activity at pH 6, with remarkable stability within the pH range of 6.0 to 8.0. The enzyme demonstrated maximum efficiency at 60 °C and considerable stability at ≤70 °C. Mg2+, Mn2+, Ba2+, Ca2+, 2-mercaptoethanol, Tween 20, Tween 40, and Triton X-100 positively influenced Xyn_TKU045, while Zn2+, Fe2+, Fe3+, Cu2+, and sodium dodecyl sulfate exhibited adverse impact. The kinetic properties of Xyn_TKU045 were a Km of 0.628 mg/mL, a kcat of 75.075 s−1 and a kcat/Km of 119.617 mL mg−1s−1. Finally, Xyn_TKU045 could effectively catalyze birchwood xylan into xylotriose and xylobiose as the major products.","PeriodicalId":36729,"journal":{"name":"Recycling","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141367624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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