Translational Medicine of Aging最新文献_第10页

Therapeutic potential of boosting NAD+ in aging and age-related diseases 提高NAD+在衰老和年龄相关疾病中的治疗潜力

Translational Medicine of Aging Pub Date : 2018-01-01 DOI: 10.1016/j.tma.2018.08.003

Yahyah Aman , Yumin Qiu , Jun Tao , Evandro F. Fang

引用次数: 53

Translational geroscience: From invertebrate models to companion animal and human interventions 转化老年科学:从无脊椎动物模型到伴侣动物和人类干预

Translational Medicine of Aging Pub Date : 2018-01-01 DOI: 10.1016/j.tma.2018.08.002

Mitchell B. Lee, Matt Kaeberlein

{"title":"Translational geroscience: From invertebrate models to companion animal and human interventions","authors":"Mitchell B. Lee, Matt Kaeberlein","doi":"10.1016/j.tma.2018.08.002","DOIUrl":"10.1016/j.tma.2018.08.002","url":null,"abstract":"<div><p>Translational geroscience is an interdisciplinary field descended from basic gerontology that seeks to identify, validate, and clinically apply interventions to maximize healthy, disease-free lifespan. In this review, we describe a research pipeline for the identification and validation of lifespan extending interventions. Beginning in invertebrate model systems, interventions are discovered and then characterized using other invertebrate model systems (evolutionary translation), models of genetic diversity, and disease models. Vertebrate model systems, particularly mice, can then be utilized to validate interventions in mammalian systems. Collaborative, multi-site efforts, like the Interventions Testing Program (ITP), provide a key resource to assess intervention robustness in genetically diverse mice. Mouse disease models provide a tool to understand the broader utility of longevity interventions. Beyond mouse models, we advocate for studies in companion pets. The Dog Aging Project is an exciting example of translating research in dogs, both to develop a model system and to extend their healthy lifespan as a goal in itself. Finally, we discuss proposed and ongoing intervention studies in humans, unmet needs for validating interventions in humans, and speculate on how differences in survival among human populations may influence intervention efficacy.</p></div>","PeriodicalId":36555,"journal":{"name":"Translational Medicine of Aging","volume":"2 ","pages":"Pages 15-29"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tma.2018.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37902200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans 一个用于秀丽隐杆线虫DNA组装、基因组工程和多色成像的工具包

Translational Medicine of Aging Pub Date : 2018-01-01 DOI: 10.1016/j.tma.2018.01.001

Bryan Sands, Nikolay Burnaevskiy, Soo R. Yun, Matthew M. Crane, Matt Kaeberlein, Alexander Mendenhall

{"title":"A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans","authors":"Bryan Sands, Nikolay Burnaevskiy, Soo R. Yun, Matthew M. Crane, Matt Kaeberlein, Alexander Mendenhall","doi":"10.1016/j.tma.2018.01.001","DOIUrl":"10.1016/j.tma.2018.01.001","url":null,"abstract":"<div><p>One way scientists can observe and quantify processes in living cells is to engineer the genomes of animals to express multiple fluorescent proteins and then quantify those signals by various imaging techniques. To allow our laboratories to confidently quantify mixed (overlapping) fluorescent signals for our studies in the basic biology of gene expression and aging in <em>C. elegans</em>, we developed a comprehensive toolkit for <em>C. elegans</em> that we describe here. The Toolkit consists of two components: 1) a series of vectors for DNA assembly by homologous recombination (HR) in the yeast, <em>Saccharomyces cerevisiae,</em> and 2) a set of ten worm strains that each express a single, spectrally distinct fluorescent protein, under control of either the <em>daf-21</em> or <em>eft-3</em> promoters. We measured the <em>in vivo</em> emission spectrum (3 nm resolution) for each fluorescent protein in live <em>C. elegans</em> and showed that we can use those pure spectra to unmix overlapping fluorescent signals in spectral images of intestine cells. Seven of ten fluorescent proteins had signals that appeared to be localized in vesicular/elliptical foci or tubules in the hypodermis. We conducted fluorescence recovery after photobleaching (FRAP) experiments and showed that these structures have recovery kinetics more consistent with freely diffusing protein than aggregates (Q35:YFP). This toolkit will allow researchers to quickly and efficiently generate mutlti-fragment DNA assemblies for genome editing in <em>C. elegans.</em> Additionally, the transgenic <em>C. elegans</em> and the measured emission spectra should serve as a resource for scientists seeking to perform, or test their ability to perform, multidimensional (multi-color) imaging experiments.</p></div>","PeriodicalId":36555,"journal":{"name":"Translational Medicine of Aging","volume":"2 ","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tma.2018.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25455432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

The functions of sarcopenia related myokines 肌少症相关肌因子的功能

Translational Medicine of Aging Pub Date : 2018-01-01 DOI: 10.1016/j.tma.2018.08.001

Lili Han , Si Wu , Ping Hu

引用次数: 7

Alternative splicing in aging and age-related diseases 选择性剪接在衰老和年龄相关疾病中的作用

Translational Medicine of Aging Pub Date : 2017-10-01 DOI: 10.1016/j.tma.2017.09.005

Huan Li , Ziyue Wang , Tianyi Ma , Gang Wei, Ting Ni

引用次数: 28

The role of RNA helicases in aging and lifespan regulation RNA解旋酶在衰老和寿命调节中的作用

Translational Medicine of Aging Pub Date : 2017-10-01 DOI: 10.1016/j.tma.2017.08.001

Sangsoon Park , Hae-Eun H. Park , Heehwa G. Son , Seung-Jae V. Lee

引用次数: 6

Serendipity in search for longevity from experiences of Hansen people 从汉森人的经历中寻找长寿的机缘巧合

Translational Medicine of Aging Pub Date : 2017-10-01 DOI: 10.1016/j.tma.2017.06.001

Sang Chul Park

{"title":"Serendipity in search for longevity from experiences of Hansen people","authors":"Sang Chul Park","doi":"10.1016/j.tma.2017.06.001","DOIUrl":"10.1016/j.tma.2017.06.001","url":null,"abstract":"<div><p>While surveying Korean centenarians, I had a fortune to encounter Hansen people, who spent long lives in the restricted sanatorium for leprosy patients. Their unexpectedly long life span compared with the normal population prompted me to examine the background and possible mechanism. Interestingly, a considerable number of these people maintain their intake of 4, 4′-diaminodiphenyl sulfone (DDS), a therapeutic drug for leprosy, habitually for more than three or four decades despite being completely cured of the disease. They have a higher antioxidant activity in their blood than age-matched controls. The protective function of DDS against oxidative stress was confirmed by <em>in vitro</em> tests and <em>in vivo</em> animal models. Moreover, DDS extended life span and increased mobility in a nematode model by inhibiting pyruvate kinase. When DDS effect on functional loss of the older people's muscles was tested, its anti-sarcopenia effect was prominent, especially in non-dominant muscles of DDS-consuming Hansen people. Concerning neurodegenerative disorders, DDS is significantly protective in animal model against Parkinson's disease. Taken together, DDS, serendipitously discovered in the Korean centenarian study, might be a candidate as a new therapeutic modality for protection against aging-dependent dysfunction in neural and muscular degeneration, and possibly for extension of lifespan.</p></div>","PeriodicalId":36555,"journal":{"name":"Translational Medicine of Aging","volume":"1 ","pages":"Pages 12-17"},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tma.2017.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44128509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Alcohol and aging: From epidemiology to mechanism 酒精与衰老:从流行病学到机制

Translational Medicine of Aging Pub Date : 2017-10-01 DOI: 10.1016/j.tma.2017.09.001

Stuart S. Adamson , Lear E. Brace , Brian K. Kennedy

{"title":"Alcohol and aging: From epidemiology to mechanism","authors":"Stuart S. Adamson , Lear E. Brace , Brian K. Kennedy","doi":"10.1016/j.tma.2017.09.001","DOIUrl":"10.1016/j.tma.2017.09.001","url":null,"abstract":"<div><p>Alcohol is one of the most widely encountered drugs in the world and is credited with a wealth of health interactions. Light to moderate regular consumption (14–28 g daily) can promote heart health, protect against Type II diabetes, and likely extend overall lifespan. However, higher consumption rates lead to the detrimental effects more widely associated with ethanol consumption, including decreased motor control, cardiotoxicity, insulin resistance, and liver disease. Despite high consumption rates in the elderly population, there has been little focus on alcohol's multifaceted effects in the context of aging. Ethanol interacts with numerous genetic targets that are already associated with aging (such as mitigation of the mechanistic target of rapamycin and activation of FOXO3A), and its dose-dependent lifespan extension depends on still poorly understood connections to these pathways. This review focuses on ethanol's relationship to aging and lifespan in multiple animal models, and it demonstrates how understanding the complicated role of this ubiquitous chemical could be vital in order to apply our knowledge of mechanisms mediating the aging process to the human population.</p></div>","PeriodicalId":36555,"journal":{"name":"Translational Medicine of Aging","volume":"1 ","pages":"Pages 18-23"},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tma.2017.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43442574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Meeting report: Fifth Asian Society for aging research symposium 会议报告：第五届亚洲老龄化研究会

Translational Medicine of Aging Pub Date : 2017-10-01 DOI: 10.1016/j.tma.2017.09.002

Takahiko Shimizu , Isao Shimokawa

引用次数: 0

Translational geroscience: A new paradigm for 21st century medicine 转化老年科学:21世纪医学的新范式

Translational Medicine of Aging Pub Date : 2017-10-01 DOI: 10.1016/j.tma.2017.09.004

Matt Kaeberlein

引用次数: 37