Biomedical and Biotechnology Research Journal最新文献

{"title":"Anti-Oxidant and anti-cancer activities of biogenic synthesized copper oxide nanoparticles","authors":"A. Agila, J. Vimala, M. Bharathy, Gnanasekar Dayana Jeyaleela, S. Sheela","doi":"10.4103/bbrj.bbrj_136_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_136_22","url":null,"abstract":"Background: The nature acts like a large “bio-laboratory” comprising plants, algae, fungi, yeast, etc., which are composed of biomolecules. These naturally occurring biomolecules have been identified to play an active role in the formation of nanoparticles. Methods: This research work mainly aims to investigate the anti-oxidant (diphenylpicrylhydrazyl assay) and anti-cancer (Michigan cancer foundation-7 cell line) capacities of biologically prepared copper oxide mediated from the hydroalcoholic extract of Justicia glauca by simple precipitation and also to identify the phytochemicals in the extract by qualitatively. Results: On screening test, the extract shows the presence of carbohydrate, phenolics, alkaloids, and terpenoids saponins which are chiefly act as a reducing, stabilizing, and capping agents in nanomaterial preparations. The medicinal plant Justicia glauca extract-mediated copper oxide materials were synthesized by lost cost, simple, effective, and eco-friendly precipitation method. The prepared copper nanomaterials were characterized by ultraviolet–visible, Fourier-transform infrared spectroscopy, energy dispersive X-ray analysis, X-ray diffraction, and scanning electron microscope. The obtained spectral results reveal that the prepared particles were found to be elliptical flat shapes of copper oxide with the average size of 19.72 nm with 51.11% of copper and 48.89% of oxygen elements. Especially, on anti-oxidant and anti-cancer activities the prepared Justicia glauca extract-mediated copper oxide revealed excellent potent while comparing the other green synthesized copper oxide particles. Conclusion: Overall results evidenced that the aqueous extract of Justicia glauca is a very good bioreductant for the synthesis of copper oxide nanoparticles.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"341 - 348"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43173666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer activity of tin oxide and cerium-doped tin oxide nanoparticles synthesized from Ipomoea carnea flower extract","authors":"M. Prasanth, Gurusamy Muruganandam, K. Ravichandran, Gnanasekar Dayana Jeyaleela, K. Shanthaseelan, Baskaran Priyadharshini","doi":"10.4103/bbrj.bbrj_100_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_100_22","url":null,"abstract":"Background: The aim of the study is to investigate the anticancer potential of tin oxide (SnO2) and different concentrations (2%, 4%, 6%, and 8%) of cerium-doped tin oxide nanoparticles (Ce-SnO2 NPs) using Ipomoea carnea flower extract. The synthesized SnO2 and different concentrations (2%, 4%, 6%, and 8%) of Ce-SnO2 NPs was tested using a colorimetric-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay against MCF-7 human breast cancer cell line cells. Methods: Collection and preparation of plant extract is preliminarily carried out followed by the synthesis of Undoped and Cerium doped Tin oxide nanoparticles is achieved by standard protocol along with that its anticancer activity also studied in this research. Results: The anticancer activity increased in direct proportion to the cerium-dopant concentration. Experimental results demonstrated that 8% Ce-SnO2 NPs exhibited a potential anticancer effect compared with SnO2 and other concentrations of Ce-SnO2 NPs. Conclusion: According to the current findings, large-scale manufacturing of Ce-SnO2 NPs might be recommended to have effective anticancer agents against breast cancer cell lines.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"337 - 340"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41546992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between the hematological indices and COVID-19","authors":"N. Khalil, B. Almugadam, Abdalkhalig Elkhider","doi":"10.4103/bbrj.bbrj_151_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_151_22","url":null,"abstract":"Background: Recently, the novel coronavirus disease-2019 (COVID-19) has a wide spread around the world. Clinically, it was responsible for respiratory illness ranged from mild to life-threatening infection. The study aimed to investigate the link of gender, age, ABO blood groups, and hematological indices with COVID-19 infection. Methods: One hindered COVID-19 cases, as confirmed using reverse transcription–polymerase chain reaction test, were checked for some sociodemographic features, blood group, and hematology parameters. A blood sample was collected from each person in an EDTA container and immediately tested for blood group using commercial antisera, in addition to complete blood count parameters using of a semiautomated hematology analyzer (Mindray BC-3200). Results: Of 100 COVID-19 patients, 52% were male and 48% were female. About 33% and 31% of the study participants were of age group 15–31 and 32–49 years, respectively. The majority (37%) of COVID-19 patients carried blood group O+ve, followed by A+ve (24%). A+ve blood group was significantly more reported in males (32.7%) than females (14.6%). Notably, 61% of participants showed low Hb level. Erythropenia was detected in 41% of the participants, while thrombocytopenia was observed only in 19% of the patients. Moreover, lymphocytopenia was detected in 82%, low packed cell volume in 66%, decreased mean corpuscular volume in 20%, and declined mean corpuscular hemoglobin concentration in 8% of the participants. In contrast, leukocytosis and neutrophilia were found in 69% and 73% of the participants, respectively. Conclusion: Taken together, the study findings highlighted the link of COVID-19 with age, gender, blood groups, and hematology parameters, which is important in diagnosis, prognosis, and management of illness.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"422 - 428"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48095464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated urinary CD80 excretion in children with steroid-responsive nephrotic syndrome","authors":"N. Hooman, H. Otukesh, R. Hosseini, A. Nickavar, F. Dastan, M. Sarouei, Parisa Honarpisheh","doi":"10.4103/bbrj.bbrj_156_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_156_22","url":null,"abstract":"Background: Idiopathic nephrotic syndrome (INS) is one of the most common glomerular diseases in children with different pathological types and different responses to corticosteroids. A definitive diagnosis is essential for planning the treatment and determining the prognosis of these patients and currently, kidney biopsy is the only method for definitive diagnosis. However, this is an invasive procedure. In addition, in some cases, the biopsy is contraindicated or tissue obtained on biopsy is insufficient and may not represent the underlying disease. According to the recent hypothesis about the role of circulating permeability factors in the pathogenesis of INS, urine protein analysis as a noninvasive method to determine the specific biomarkers of the disease is of great interest to nephrologists and can be useful. Methods: In this case − control study, we analyzed urinary CD80 (uCD80) levels in 51 patients with INS using a special CD80 enzyme-linked immunosorbent assay kit and were compared between different groups of patients. Results: The highest urine CD80/creatinine ratio was found in patients with active minimal change disease and steroid-responsive nephrotic syndrome in the relapse stage of the disease. Conclusion: A significant level of uCD80 is correlated with better renal function and a more favorable response to steroids in patients with INS. Therefore, it can be concluded that a high level of uCD80 is correlated with a good prognosis in these patients.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"367 - 371"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45198781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of chemometric-assisted spectrophotometric method for the simultaneous estimation of aceclofenac, paracetamol, and chlorzoxazone with impurities","authors":"Rajshree Gunjal, Arti V. Gajbhar, V. Vichare, A. Sutar, M. Deshmukh, V. Choudhari","doi":"10.4103/bbrj.bbrj_92_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_92_22","url":null,"abstract":"Background: Analysis of tertiary mixtures of analytes along with their impurities with simple and cost effective manner is always of interest. Utility of chemometric techniques are growing in pharmaceuticals, it improve speediness in the analysis and also provide analytical solutions with reduce the number of steps in the analytical method. In this study UV-Visible spectrophotometry coupled with principle component regression (PCR) and partial least square (PLS) multivariate methods was applied for estimation of three drugs in their formulation. Method: The calibration and validation sets were prepared in linear concentration range of three drugs and major impurities of paracetamol and aceclofenac. The series of sets were prepared using multilevel multifactorial design. Leave- One-Out (LOO) cross validation technique was employed to get essential number of Latent variables (LVs) that provides the greatest predictive ability. The developed method was studied for qualitative and quantitative analysis of titled drugs and validated as per regulatory guidelines. Results: The results showed the values of coefficient of determination (R2) for all drugs and impurities was higher than 0.99 indicating high acceptability. The obtained RMSE values were relatively low. Coefficient of determination and RMSE values indicate good accuracy and precision, respectively. Conclusion: Proposed method was successfully used for analysis of aceclofenac, paracetamol and chlorzoxazone in tablet dosage form and major impurities of aceclofenac, paracetamol in bulk.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"458 - 465"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47449526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of some medicinal plants and antibiotics against Staphylococcus aureus and Mammaliicoccus sciuri isolated from acne","authors":"Meghali Goswami","doi":"10.4103/bbrj.bbrj_135_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_135_22","url":null,"abstract":"Background: Acne vulgaris is a distressing condition that affects most adolescents, causing scarring and reducing the quality of life. Among all the available therapeutic options, antibiotics are routinely used to treat moderate acne. In some cases, the clinical symptoms temporarily disappear after applying the prescribed medications, but its reoccurrence along with the misuse and overuse of the prescribed antibiotics may result in the emergence of multidrug-resistant strains. Methods: The present study aims to isolate and identify acne-causing bacteria from two individuals, who developed chronic or recurrent papules or pustules on their face and neck. The effect of different physiological factors on the growth of these bacteria was evaluated, followed by an evaluation of microbial adhesion, biofilm formation, hemolytic activity, extracellular enzyme production, and antibacterial activity of some medicinal plants and antibiotics. Results: The predominant bacteria in acne samples were Gram-positive cocci, which were identified as Staphylococcus aureus strain GCC20_MS and Mammaliicoccus sciuri strain GCC20_MS. Both the isolates showed maximum viability at pH 7.0 and significant growth up to 10% NaCl concentration. A decreased viable count with the increase in Lysozyme concentration was also observed. The cell-surface hydrophobicity and auto-aggregation of both the tested isolates were very low; however, the strains were moderate biofilm producers. S. aureus strain GCC_20MS exhibited β-hemolysis, whereas M. sciuri strain GCC_20RS showed γ-hemolytic activity (no-hemolysis). Antibiotic-sensitivity test showed resistance of both the strains toward penicillin and sensitivity toward tetracycline, chloramphenicol, ciprofloxacin, levofloxacin, co-Trimoxazole, gentamicin, and ofloxacin. The aqueous extracts of Citrus limon and Psidium guajava significantly inhibit the growth of the isolated strains. Moderate growth inhibition was observed by the aqueous extracts of Mentha sachalinensis and Punica granatum. Conclusion: The study suggests the efficacy of topical anti-acne formulations using plant extracts that may target the early processes of acne development and combat the emergence of multidrug-resistant strains.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"372 - 381"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41426562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico Study of the interaction of fucoidan with thrombolytic agents","authors":"V. Suprunchuk","doi":"10.4103/bbrj.bbrj_121_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_121_22","url":null,"abstract":"Background: Tissue plasminogen activator (tPA) is one of the most widely used drugs in thrombolytic therapy. However, due to the inactivation of tPA in the bloodstream and increased risk of bleeding with increasing tPA dosages, the development of targeted delivery systems of tPA is required. For these purposes, it is possible to use fucoidan. The aim of the work was to study the possibility of forming of tPA-fucoidan conjugates and maintaining the activity of the enzyme using molecular docking. Methods: Docking simulations between tPA and fucoidan were performed by use of a docking software AutoDock tools version 1.5.7 and AutoDock 4.2.6. Using “blind docking” to identify the centers of molecular docking approaches of the enzyme (tPA) with the ligand (the active part of the fucoidan structure), as well as to establish the influence of the ligand on the active site of the enzyme. Results: Two “hot spots” of fucoidan binding to the enzyme were found: the region containing SER85-CYS97 residues and the region containing PHE217-TYR223 residues. This interaction can lead to the successful binding of the enzyme and polysaccharide to form a protein-polysaccharide complex. In this case, there may be a lack of suppression of the action of tPA. The interaction with the ligand was found to occur on the surface of the protein molecule. Conclusions: In this study, coupling simulations of interactions of tPA with fucoidan were conducted. The resulting conjugate can be used in the development of systems for the targeted delivery of a thrombolytic agent. This study predicts that the formation of tPA-fucoidan conjugate is a promising approach for optimizing treatment strategies for thrombosis.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"349 - 352"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42565082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of silver nanoparticles using Nigella sativa seed extract and its efficacy against some multidrug-resistant uropathogens","authors":"C. Ezeh, C. Eze, M. Dibua, S. Emencheta, C. Ezeh","doi":"10.4103/bbrj.bbrj_104_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_104_22","url":null,"abstract":"Background: Urinary tract infection (UTI) is one of the most chronic infections in humans, as well as the most common cause of multidrug-resistant (MDR) pathogen emergence, necessitating the quest for stringent antibiotic treatment. In the imperative search for effective drugs to combat MDR, silver nanoparticles (AgNPs) are given priority. The objective of this study was to synthesize, characterize, and evaluate the antimicrobial activity of AgNPs synthesized using Nigella sativa on MDR uropathogens. Methods: Urine samples of suspected UTI patients were collected using sterile sample bottles and cultured on different agar media to isolate and identify uropathogens using conventional method. All isolates were screened for multidrug resistance by disk diffusion method following the Clinical and Laboratory Standards Institute guideline with slight modification. N. sativa seed extract was used to synthesize AgNPs from aqueous solution of silver nitrate (AgNO3). AgNPs formed were characterized using ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscope (SEM), dynamic light scattering, Fourier transform infrared spectroscopy, and X-ray diffraction spectroscopy (XRD). Antibacterial activities of synthesized AgNPs were assessed (in vitro) by disc diffusion method against MDR isolates, and cytotoxicity was evaluated using brine shrimp lethality assay. The formed AgNPs were characterized using UV-Vis, and antibacterial activity of synthesized AgNPs was assessed (in vitro) by impregnated disc diffusion method against MDR isolates. Results: Escherichia coli, Klebsiella sp., and Staphylococcus aureus were isolated. Multiple antibiotic-resistant indexes of the isolates ranged between 0.4 and 0.75 against the different standard antibiotics tested. The color change from pale yellow to dark brown was indicative of AgNP formation. UV-Vis spectrum of solution containing AgNPs exhibited peak wavelengths of 378 nm. Fourier transform infrared spectroscopic analysis showed that terpenoids, flavonoid, and phenols played an important role in the biosynthesis. Irregular shapes within nanoscale range were detected using SEM. XRD showed that the particles were crystalline in structure with an average size of 32 nm. The mean diameter zone of inhibition (in mm) for the different isolates at the dose of 100 μg/mL concentration showed maximum for E. coli (18 mm) followed by both Klebsiella sp. and S. aureus at 17 mm. Conclusion: The study underscores the efficacy of the plant-mediated nanoparticles as well as their potential for use as broad-spectrum antimicrobial agents for the management of MDR uropathogens.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"400 - 409"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70692295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of atrial natriuretic peptide in abrogated cardio protective effect of ischemic postconditioning in diabetic rat heart","authors":"G. Gupta, V. Varshney, Ahsas Goyal, J. Gupta, H. Yadav","doi":"10.4103/bbrj.bbrj_90_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_90_22","url":null,"abstract":"Background: Diabetes decreased cardioprotective potential of ischemic postconditioning (IPOC), atrial natriuretic peptide (ANP) induced the cardioprotection against ischemic-reperfusion (I/R) injury. The present study has been designed to investigate the role of ANP induced postconditioning in diabetic rat heart. Methods: Isolated Langendorff perfused normal and diabetic rat hearts were stabilized for 10 min proceed by global ischemia further followed by four cycles of IPOC, each cycle comprised 5 min of reperfusion and 5 min of ischemia at onset of 120 min of reperfusion. Perfusion of ANP (0.1μM/l) with Krebs–Henseleit Buffer solution in isolated diabetic rat heart for four-cycle of IPOC significantly decreased I/R-induced myocardial infarct size and release of CK-MB and lactate dehydrogenase (LDH) level in coronary effluent. Results: Four cycles of IPOC-induced cardioprotection noted by decreased in infarct size and also in release of LDH and CK-MB in normal rat heart. However, IPOC-induced cardioprotection was completely attenuated in isolated heart obtained from diabetic rat. Perfusion of ANP (0.1μM/L) significantly restored the attenuated cardioprotection in diabetic rat heart, which was completely blocked by perfusion of L-NAME (100μM/L), an eNOS inhibitor. Conclusion: So that, ANP restored cardioprotective affect in diabetic rat heart, which was completely abolished by the perfusion of L-NAME (100μM/L), an eNOS inhibitor.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"416 - 421"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44751527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effect of Basella alba-Mediated zinc oxide nanoparticles against the infection-causing bacteria","authors":"A. Agila, Gnanasekar Dayana Jeyaleela, J. Vimala, M. Bharathy, S. Sheela","doi":"10.4103/bbrj.bbrj_144_22","DOIUrl":"https://doi.org/10.4103/bbrj.bbrj_144_22","url":null,"abstract":"Background: Naturally occurring biomolecules from the plant extract have been identified to play an active role in the formation of any nanoparticles. Methods: This work aimed to synthesize the nano-sized zinc oxide material (zinc oxide nanoparticles [ZnONPs]) using the 70% ethanolic leaf extract of Basella alba by precipitation method and also studied the antibacterial activity of green-synthesized ZnONPs on infection-causing five bacteria. Synthesized nanomaterials were characterized by the aid of ultraviolet (UV)-visible, Fourier transform infrared spectroscopy, scanning electron microscope, X-ray diffraction, and Energy Dispersive X-ray Analysis (EDAX). Results: Qualitative analysis and UV results of extract reveal the occurrence of some medicinally important phytomolecules such as flavonoids, terpenoids, phenolic acids, and ascorbic acid. The spherical nature of ZnONPs was observed with an average crystalline size of 28.64 nm. EDAX analysis revealed the elemental compositions in the B. alba-mediated ZnONPs (BA-ZnONPs) which showed zinc in 70.04% and oxygen in 29.96%. BA-ZnONPs were tested against the bacteria (an infection causing) such as Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, Staphylococcus aureus, and Proteus vulgaris, which results that, against all the pathogens, ZnONPs showed noticeable inhibition effects compared with zinc acetate and B. alba extract. Especially against the E. coli, ZnONPs performed well with inhibitory effect and least on S. aureus. Conclusion: Antibacterial activities of BA-ZnONPs were studied which can act as the new antimicrobial-resistant agents.","PeriodicalId":36500,"journal":{"name":"Biomedical and Biotechnology Research Journal","volume":"6 1","pages":"353 - 359"},"PeriodicalIF":1.4,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44953347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}