Acta Marisiensis - Seria Medica最新文献_第9页

The influence of transport condition and processing time on plasma ammonia results 运输条件和处理时间对血浆氨的影响

Acta Marisiensis - Seria Medica Pub Date : 2022-03-01 DOI: 10.2478/amma-2022-0005

Karina-Alexandra Cojocaru, O. Oprea, M. Dobreanu

{"title":"The influence of transport condition and processing time on plasma ammonia results","authors":"Karina-Alexandra Cojocaru, O. Oprea, M. Dobreanu","doi":"10.2478/amma-2022-0005","DOIUrl":"https://doi.org/10.2478/amma-2022-0005","url":null,"abstract":"Abstract Objective: Ammonia is extremely unstable in blood specimens and has special requirements during transport, processing and storage. The aim of our study was to determine the stability of ammonia in EDTA K3 blood samples and to establish a protocol for sample handling. Methods: In this study, 36 healthy subjects and 47 inpatients diagnosed with type 2 diabetes mellitus were enrolled. Two peripheral blood samples were collected from healthy volunteers (Sample A1 and A2) and one peripheral blood sample was collected from the inpatients diagnosed with type 2 diabetes mellitus (Sample B). Sample A1 and B were transported in ice bath within 15 minutes of blood collection, centrifuged immediately and processed. The sample was re-centrifuged after 15 minutes and a second ammonia result was obtained. Sample A2 was transported at room temperature and stored between 2 and 4 hours, centrifuged and plasma ammonia measurement was performed. The sample was re-spun after 15 minutes and a fourth ammonia result was obtained. Results: In our study, in healthy group the difference between sample A2 and set point value (on ice, 15 minutes) is 25.08 µg/dl, showing an increase of 55.29%. After another 15 minutes, an increase of 82.02% was observed compared with the standard value. In diabetes mellitus group, after 30 minutes of blood collection, an increase of 11% over the set point value was observed. Conclusions: The blood specimen should be transported on ice to the laboratory and analyzed within 15 minutes of blood collection due to plasma ammonia spontaneously increase.","PeriodicalId":36282,"journal":{"name":"Acta Marisiensis - Seria Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81178869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emotional disorders associated to multiple sclerosis and psychological interventions: a literature review 与多发性硬化症相关的情绪障碍和心理干预:文献综述

Acta Marisiensis - Seria Medica Pub Date : 2022-03-01 DOI: 10.2478/amma-2021-0042

Alina Schenk, C. Popa, C. M. Cojocaru

引用次数: 0

Chest wall secondary chondrosarcoma caused by malignant degeneration of an enchondroma: case report and literature review 由内生纤维瘤恶性变性引起的胸壁继发性软骨肉瘤1例报告并文献复习

Acta Marisiensis - Seria Medica Pub Date : 2022-03-01 DOI: 10.2478/amma-2022-0006

C. Ioniță, I. Cojocaru, A. Gomotirceanu, B. Suciu, D. Milutin, A. A. Molnar, Vlad Neag, I. Halmaciu

引用次数: 0

Apathy as Non-Motor Manifestation in Amyotrophic Lateral Sclerosis 肌萎缩性侧索硬化症的非运动表现为冷漠

Acta Marisiensis - Seria Medica Pub Date : 2022-03-01 DOI: 10.2478/amma-2021-0041

Anca Moţăţăianu, Ioana Ormenișan, R. Bălaşa

引用次数: 0

The antibacterial potential of biosynthesized silver nanoparticles using beech bark and spruce bark extracts 用山毛榉树皮和云杉树皮提取物合成的纳米银的抗菌潜力

Acta Marisiensis - Seria Medica Pub Date : 2022-03-01 DOI: 10.2478/amma-2021-0043

Anca Mare, A. Man, F. Toma, Bianca Tudor, Lavinia Berța, C. Tanase, C. Ciurea

{"title":"The antibacterial potential of biosynthesized silver nanoparticles using beech bark and spruce bark extracts","authors":"Anca Mare, A. Man, F. Toma, Bianca Tudor, Lavinia Berța, C. Tanase, C. Ciurea","doi":"10.2478/amma-2021-0043","DOIUrl":"https://doi.org/10.2478/amma-2021-0043","url":null,"abstract":"Abstract Introduction: Lately, nanotechnology focuses on the green synthesis of AgNPs, using different plant materials, as this method is accessible, cost-efficient, and ecological. The study aimed to investigate the antibacterial potential of AgNPs synthesized using beech/spruce bark extracts (BBE/SBE) and silver salts (acetate/nitrate). Method: The growth rates of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 were evaluated in the presence of the AgNPs solutions. The checkerboard method was performed to evaluate if these solutions exert synergistic activity with gentamicin. Results: For E. coli, synergistic effects were observed for the combination of gentamicin 0.25mg/L with AgNP BBE Nit (0.145mg/mL) and with AgNP SBE Ac (0,09mg/mL). For S. aureus, no synergistic effects were observed. Overall, the AgNP BBEs solutions combined with gentamicin presented lowest values of fractional inhibitory concentration than the ones registered for the combination of AgNP SBEs with gentamicin, for both bacterial strains. The growth rate of S. aureus was inhibited by all the tested AgNPs at the measured time points. For E. coli, after 24 hours of incubation, the growth rate was inhibited only in the presence of AgNP SBE Ac. After 6 hours of incubation, the growth rate of E. coli was almost stationary in the presence of AgNP BBE Nit. Conclusions: The biosynthesis of AgNPs is a valuable choice for obtaining substances with antibacterial potential.","PeriodicalId":36282,"journal":{"name":"Acta Marisiensis - Seria Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76029285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mediation of Candida species growth and virulence by the proinflammatory cytokine IL-6 促炎细胞因子IL-6对念珠菌生长和毒力的调节作用

Acta Marisiensis - Seria Medica Pub Date : 2021-12-01 DOI: 10.2478/amma-2021-0036

C. Ciurea, I. Kosovski, F. Toma, M. Mareș, Bianca Tudor, A. Man

{"title":"Mediation of Candida species growth and virulence by the proinflammatory cytokine IL-6","authors":"C. Ciurea, I. Kosovski, F. Toma, M. Mareș, Bianca Tudor, A. Man","doi":"10.2478/amma-2021-0036","DOIUrl":"https://doi.org/10.2478/amma-2021-0036","url":null,"abstract":"Abstract Interleukin-6 (IL-6) is a cytokine with pleiotropic effects that might also influence the virulence traits of some microorganisms, but its direct influence over Candida spp. is currently unknown. The objective of the study is to determine the influence of IL-6 (250 pg/ml) on the growth rate and biofilm formation of C. albicans, C. parapsilosis, C. krusei, C. auris, and C. guilliermondii, as well as to analyze the influence of this citokine on the expression of three virulence genes (ALS3, HSP70, SAP2), respectively on the germ tube formation ability of C. albicans. The influence of IL-6 on growth rate was assessed by incubating the fungal cells in presence of IL-6 for 48 hours and assessing the optical density of the samples at five timepoints. The biofilm production in presence of IL-6 was studied in microtiter plates, using crystal-violet assay, the intensity of biofilms being evaluated by spectrophotometry. The expression of ALS3, HSP70, and SAP2 in C. albicans was studied by RT-PCR, reported to ACT1 housekeeping gene. The germ-tube test was performed to assess the influence of IL-6 on the filamentation rate of C. albicans. All test results were normalized against control, without added IL-6. The results showed that IL-6 influences the growth rate of C. albicans, C. parapsilosis, C. krusei, C. auris, and C. guilliermondii in a time-dependent way. Also, IL-6 inhibited the formation of biofilms for C. albicans and C. guilliermondii. In C. albicans cells, IL-6 induced upregulation of ALS3 and HSP70, while it down-regulated the SAP2 gene. IL-6 did not influence the germ-tube formation in C. albicans. In conclusion, IL-6 might exert, in vitro, direct effects on the virulence traits of Candida spp., and its influence is dependent on the exposure time. Non-albicans Candida species presented particular responses to IL-6.","PeriodicalId":36282,"journal":{"name":"Acta Marisiensis - Seria Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73226487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prosthodontic Management of Dentate Maxillectomy Patient: A Clinical Case Report 上颌有牙切除患者的修复处理:1例临床报告

Acta Marisiensis - Seria Medica Pub Date : 2021-12-01 DOI: 10.2478/amma-2021-0038

Vineet Sharma, Jyoti Paliwal, K. Meena, R. L. Raigar

引用次数: 0

Formulation and evaluation of controlled release ocusert of gatifloxacin and prednisolone 加替沙星强的松龙控释剂的研制与评价

Acta Marisiensis - Seria Medica Pub Date : 2021-12-01 DOI: 10.2478/amma-2021-0032

N. Nagpal, Sukhmanpreet Singh, Guraasdeep Kaur, Parminderjit Kaur, R. Dhawan, Mandeep Singh Chhina, Manisha Arora

{"title":"Formulation and evaluation of controlled release ocusert of gatifloxacin and prednisolone","authors":"N. Nagpal, Sukhmanpreet Singh, Guraasdeep Kaur, Parminderjit Kaur, R. Dhawan, Mandeep Singh Chhina, Manisha Arora","doi":"10.2478/amma-2021-0032","DOIUrl":"https://doi.org/10.2478/amma-2021-0032","url":null,"abstract":"Abstract Objective: Ocuserts are sterile, solid, or semisolid dosage forms prepared to attain increased contact time between the drug and the conjunctival tissue to keep up a constant release of drug when placed in the lower cul-de-sac or conjunctival sac of the eye. The aim of formulating this delivery system was to treat both inflammatory and infectious conditions of the eye with increased ocular residence time by releasing drugs at a slow and constant rate. Method: Gatifloxacin, and prednisolone ocuserts were prepared by solvent casting method, and evaluated for physical appearance, uniformity of weight, thickness, folding endurance, drug content, surface pH, in-vitro, and ex-vivo release profile. Results: All formulated inserts exhibited positive results in terms of their evaluation parameters. Ocuserts were sterile, with no turbidity in selected media during the study, and they were stable throughout six months. Conclusion: Results suggested that prepared optimized ocusert formulation would be a suitable alternative to eye drops for treating conjunctivitis and other bacterial infections.","PeriodicalId":36282,"journal":{"name":"Acta Marisiensis - Seria Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79468041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the anxiolytic effect of propranolol and dextromethorphan in mice using a model of psychogenic stress 心得安和右美沙芬对小鼠心理应激模型的抗焦虑作用研究

Acta Marisiensis - Seria Medica Pub Date : 2021-12-01 DOI: 10.2478/amma-2021-0034

Radion Garaz, Sorina Cucuiet, Gabriela Garaz-Pislariu, Raluca-Ioana Buliga

{"title":"Study of the anxiolytic effect of propranolol and dextromethorphan in mice using a model of psychogenic stress","authors":"Radion Garaz, Sorina Cucuiet, Gabriela Garaz-Pislariu, Raluca-Ioana Buliga","doi":"10.2478/amma-2021-0034","DOIUrl":"https://doi.org/10.2478/amma-2021-0034","url":null,"abstract":"Abstract Objective: To find the anxiolytic effects in a psychogenic stress model in mice, the present study has investigated the interaction between propranolol and dextromethorphan. Methods: 50 Albino Swiss male mice were housed in groups of 10 per cage. The beta-adrenergic receptor blocker propranolol (20 mg/kg bw), the N-methyl-D-aspartate (NMDA) receptor dextromethorphan (30 mg/kg bw), and their combination were administered 10 minutes after exposure to predator odor. The treatments included diazepam as positive control and normal saline as negative control. Anxiety-like behaviors were evaluated using the elevated plus-maze test (EPM) 7 days after stress induction. Results: Regarding the length of stay (F=25.53; p<0.0001)/number of entries in the open arms (F=3.533; p=0.0416), time (F=6.127; p=0.0045)/number of entries in the closed arms (F=5.690; p=0.0141), time in center-point (F=3.577; p=0,0295), and total distance traveled (F= 4.711; p=0.0145), there was a significant difference among the treated groups. Propranolol and dextromethorphan treated groups expressed lower time in the closed arms vs Placebo (p=0.0089, respectively p=0.0111). In addition, the time spent in the open arms was higher in propranolol group vs placebo group (p=0.0215). Conclusions: Considering obtained data, there was a decrease of symptoms in the sympathetic nervous system and the psychological stress disappeared in mice applying a treatment of 20 mg/kg bw propranolol. Our findings indicated that dextromethorphan partially mediated the anxiolytic-like activity. However, the combination of these two drugs did not express anxiolytic effects.","PeriodicalId":36282,"journal":{"name":"Acta Marisiensis - Seria Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88748219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ulnar-basilic arteriovenous fistula with multilocular gigantic aneurysmal dilatation: a case report 尺基底动静脉瘘合并多房巨大动脉瘤扩张1例

Acta Marisiensis - Seria Medica Pub Date : 2021-12-01 DOI: 10.2478/amma-2021-0035

Emil-Marian Arbănași, E. Russu, A. Mureṣan, Eliza-Mihaela Arbănași, Réka Kaller

引用次数: 5