Journal of Advanced Biotechnology and Experimental Therapeutics最新文献

{"title":"Effect of moderate-intensity treadmill exercise under different ambient temperatures on peripheral circulatory responses in young healthy adults","authors":"Sunao Wada, M. Mahbub, Natsu Yamaguchi, R. Hase, Yuki Nakagami, Hidekazu Takahashi, Hiroyuki Saito, J. Shimokawa, Rie Watanabe, T. Tanabe","doi":"10.5455/jabet.2023.d120","DOIUrl":"https://doi.org/10.5455/jabet.2023.d120","url":null,"abstract":"Exercise training has the potential for inducing enhancements in peripheral circulation, which can play important preventive and therapeutic roles in peripheral circulatory diseases. However, the relevant published studies show conflicting and inconclusive results. Furthermore, useful or optimum ambient temperature for this purpose has not yet been established. Therefore, we investigated the acute responses in peripheral circulation from exposure of healthy subjects to treadmill exercise under different ambient temperatures; A total of 12 young adult volunteers (males 6, females 6) randomly underwent three sessions of treadmill exercise for 30 min under three different ambient temperatures (10°C, 20°C, and 30°C), at a predetermined exercise intensity. Before and after the intervention, leg skin blood flow (SBF) was measured by laser Speckle flowgraphy and hand skin temperature (ST), by digital thermometry; After the cessation of treadmill exercise, compared to the corresponding baseline values, a significant increase in SBF was observed under all ambient temperature conditions (P<0.005). During intervention, ST showed a significant decrease at 10th min of intervention under all ambient temperatures (P<0.005) with a subsequent increasing trend in it. After intervention, a significant increase in ST was observed under 20°C condition only (P<0.05). Also, after intervention, the observed increase in systolic blood pressure was less significant under 20°C condition; Treadmill exercise appears to be a useful intervention modality in inducing improvements in peripheral circulation. However, exposure to treadmill exercise under or near 20°C ambient temperature might be recommended for the purpose.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of serum level of interleukin-33 and insulin resistance in overt and subclinical hypothyroidism patients","authors":"Z. Jabbar, H. Ali, R. Ali, M. Alrufaie","doi":"10.5455/jabet.2023.d150","DOIUrl":"https://doi.org/10.5455/jabet.2023.d150","url":null,"abstract":"Hypothyroidism is a condition that occurs when the thyroid gland fails to produce an adequate amount of the hormone thyroid, as a result of factors for example hypothalamic or pituitary gland disease, common tissue resistance to thyroid hormones, and thyroid gland diseases. It’s the most prevalent thyroid disorder. Interleukin-33 (IL-33) is a nuclear cytokine of the IL-1 family members that is abundantly expressed during homeostasis and inflammation in endothelial, epithelial, and fibroblast-like cells. It is worth noting that higher IL33 levels have been associated with insulin resistance. Aims the study: Its purpose was to examine the correlation between insulin resistance and IL33 in patients with hypothyroidism by measuring their IL33 levels. In a case-control study, 180 people were recruited and split into three categories: those with overt hypothyroidism (60), those with subclinical hypothyroidism (60), and those who were otherwise healthy (60). were of a similar age range to the patient (between 20 and 55), with 55.5% female participants and 44.5% male participants.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals and anti-hemorrhoidal activities Tapak Liman (Elephantopus scaber) leaves","authors":"Riski Sulistiarini, Anggi Puranti, W. Prabowo","doi":"10.5455/jabet.2023.d139","DOIUrl":"https://doi.org/10.5455/jabet.2023.d139","url":null,"abstract":"Hemorrhoids occur due to inflammation of the rectoanal venous plexus, which causes inflammation, pain, and can cause lumps. This inflammation can cause difficulty in defecation and can even cause heavy bleeding. Empirically Tapak Liman (Elephantopus scaber) leaves have been used to treat hemorrhoids and have been known to contain flavonoids and terpenoid lactones which are active as an anti-inflammatory. Objective: To determine the anti hemorrhoidal activity of E. scaber leaves in reducing inflammation in hemorrhoids in Wistar rats. Material and method: The research begins with the extraction of E. scaber leaves with 70% ethanol as solvent. Extracts were analyzed for total flavonoid content with quercetin standard. Antihemorrhoidal activity was measured based on the degree of edema formed based on the weight of the rectum through the surgical process. Rats were induced with croton oil on the anorectal area for three days. The treatments for the test group were 50, 100, and 200 mg/kgW. The drug control group used oral diclofenac sodium for seven days. Result: E. scaber leaf ethanol extract contains alkaloids, flavonoids, saponins, and polyphenols. The flavonoid level was 3.433mgQE/g extract. This study showed that E. scaber leaf extract had the best anti hemorrhoidal activity at a dose of 100 mg/kgW with a significance value of p<0.05. Conclusion: The ethanol extract of E.scaber leaves containing flavonoids provides a fairly good anti-hemorrhoid activity compared to the control drug diclofenac sodium.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inorganic arsenic exposure during pregnancy affects post-natal growth, blood parameters, and organ development of mice offspring","authors":"M. Basher, S. Sarkar, M. Mobarak, M. Islam","doi":"10.5455/jabet.2023.d140","DOIUrl":"https://doi.org/10.5455/jabet.2023.d140","url":null,"abstract":"Arsenic is a potentially toxic agent for human health due to its widespread presence in the environment. Arsenic poisoning from drinking contaminated groundwater has become one of Bangladesh's most difficult healthcare problems. However, there is a lack of understanding of the detrimental impact of arsenic toxicity on children of arsenic-exposed parents. This study evaluates the effect of arsenic toxicity on body growth, blood parameters, and organ development of F1 mice. In this study, adult female mice were exposed to sodium arsenite from gestation day 12.5 until parturition, and then the postnatal growth, blood indices, and organ development were assessed. The result showed that from birth to weaning offspring of the sodium arsenite-treated group exhibited significant (p<0.002, p<0.0001, p<0.0001 consecutively) and weaning to sexual maturity of female offspring significantly (p<0.01, p<0.01, p<0.0001 respectively) slower increase in final body weight, total body weight gain, and rate of body weight gain than that of the offspring of the control group. Hematological tests revealed a significant reduction (p<0.03, p<0.02) in RBC and WBC count while significant elevation (p<0.02) in Platelet count, MCV, MCH, and MCHC in offspring of sodium arsenite exposed female mice than the offspring obtained from control female mice. RBS and Triglycerides levels were significantly higher (p<0.05, p<0.0008 respectively), while Alkaline phosphatase level was significantly lower (p<0.02) in the offspring of sodium arsenite-exposed female mice than in the offspring obtained from control female mice. In terms of organ-to-body weight ratio, the female F1 mice from the exposed group demonstrated a significantly lighter (p<0.01) kidney and heart as compared to that of the female F1 mice from the control group. Visual inspection of the organ morphology showed a slightly affected liver, lungs, and testes. Overall, the study suggests that inorganic arsenic exposure of the parent mice exerts harmful effects on the body growth, hematological and biochemical parameters, and organ morphology of the offspring.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic effect of zinc oxide nanoparticle and diode laser combination in colorectal cancer in vitro via induction of cell cycle arrest and apoptosis","authors":"Chrakhan Rahman, Twana Mustafa","doi":"10.5455/jabet.2023.d146","DOIUrl":"https://doi.org/10.5455/jabet.2023.d146","url":null,"abstract":"The fourth deadliest reason for cancer-related deaths worldwide was found to be colorectal cancer (CaCo2). Zinc oxide nanoparticles (ZnO-NPs) have been researched for biomedical applications due to their demonstrated anticancer, antidiabetic, antibacterial, and anti-inflammatory. by way of the cytotoxic reactive oxygen species (ROS) that are produced when photosensitizers (PSs) are activated by light, which in turn cause cancer cell death, photodynamic treatment (PDT) causes selective cytotoxicity to malignant cells. The cytotoxic effect of different concentrations and wavelengths was studied on CaCo2, and the use of combination therapy to reduce the percentage of cell lines had clear effects on early apoptosis (p-value<0.05). The half-maximal inhibitory concentration (IC50) for the cell was evaluated. The proper IC50 for CaCo2 was 20 ug/ml and the target of Diode laser power is 5 5 j/cm 2. In the G2 phase of the cell cycle, a lower percentage of cells relative to the S and G1 phases was significantly different when laser and ZnO2 were combined (p-value<0.05). Real-time Polyclonal chain reaction (PCR); significantly substantial variation in the use of a diode laser and ZnO2 to stop the spread of cancer by boosting the expression of the p53 gene (p-value<0000). Bax a central cell death regulator, was expressed significantly (p-value<0.05). Even so, utilizing the laser and ZnO2 together did not reveal any substantial differences in the Bcl2 gene (p-value>0.05). In conclusion, this study supported that combination therapy was cytotoxic to CaCo2 in vitro and there is a possibility of developing it as an effective therapeutic agent.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ocimum basilicum extract modulates Tau aggregation and improves memory function in a neurodegenerative rat model","authors":"Mrwa Shalan, Layla Alhasan","doi":"10.5455/jabet.2023.d162","DOIUrl":"https://doi.org/10.5455/jabet.2023.d162","url":null,"abstract":"The main objective of this study was evaluation of the neuroprotective potential effect of Ocimum basilicum on oxidative stress status in rat induced Alzheimer's disease. Fifty rats were divided into five groups (ten rats each). Rats were treated orally with AlCl3 to induced AD. Group 1 (control group). Group 2 (AD group): supplemented orally with AlCl3 (17mg/kg/day) for four weeks. Group 3 (OB/AD group) supplemented concomitantly with oral rivastigmine (3 mg/kg /day). Group 4 (OB/AD group) supplemented concomitantly with oral OB (250mg/kg/day) and Group 5 (OB/AD group) supplemented concomitantly with oral OB (500 mg/kg/day). The results showed that the plant leaves extract increased serum superoxide dismutase (SOD) with a significant decrease of a serum MDA and also the aggregation of tau protein expression was decreased . Histological changes were observed in brain tissues of AD rats. However, the high dosing of the plant leaves extract (500 mg/kg) was more powerful than the low treatment with low dose (250 mg/kg) by decreasing Tau protein expression. The results suggest that Ocimum basilicum can relieve symptoms and prevent the progression of AD severity by improving memory function. It can be concluded that OB leaves were alleviated the memory impairment and learning abilities due to antioxidants activity of flavonoids, tannins and terpenoids.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135058847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence, a powerful tool to combat antimicrobial resistance: An update","authors":"Rony Masud, Naeem Fahim, Md Rana, Md Islam, Md Rahman","doi":"10.5455/jabet.2023.d161","DOIUrl":"https://doi.org/10.5455/jabet.2023.d161","url":null,"abstract":"Antimicrobial resistance (AMR), also known as antibiotic resistance, is a global health crisis. Many people die from diseases caused by multi-drug resistant pathogens (MDR). Artificial intelligence (AI) deals with applying theory and developing computer systems to conduct tasks usually requiring human intelligence, such as visual perception, speech recognition, decision-making, and translation between languages to solve problems. AI has been successfully used in various fields, from single-cell study to space science. There is huge potential for using AI in medical science, including fighting against MDR to combat AMR. In this review, we have highlighted some of AI’s successful uses and potentiality to combat AMR for the betterment of the human race, including the basic principles of AI, available AI resources, use and scope, advantages and limitations.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135802052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological burden, risk factors, and recent therapeutic advances in chronic obstructive pulmonary disease","authors":"Ayilya Loganathan, N. Abdul","doi":"10.5455/jabet.2023.d110","DOIUrl":"https://doi.org/10.5455/jabet.2023.d110","url":null,"abstract":"Chronic Obstructive Pulmonary Disease (COPD) is a progressive disease that is certainly preventable and treatable and is caused due to the continuous exposure to noxious substances and toxic gases and is characterized by airflow limitation and chronic inflammation in the lungs coupled with persistent symptoms in the respiratory tract leading to obstructive bronchiolitis and parenchymal emphysema. The incidence and progression of COPD is a complicated pathological phenomenon and the overall severity is due to its exacerbations and comorbidities in individuals. Further, COPD is a major contributor to the global years of life lost and by 2030 it would be the third leading causes of mortality in the world. Also, knowledge on COPD, its associated conditions and the clinical understanding of the disease date back to the 16th century. The prevalence, morbidity and mortality may vary across the globe based on their exposure to smoking, tobacco, occupational pollutants, indoor pollution, outdoor air pollution, gender, age and genetic inheritance. Considering the continuous exposure to toxic substances and aging of the general population, the burden and prevalence of COPD are estimated to increase substantially in the coming years. Furthermore, COVID-19 patients with pre-existing COPD conditions suffer from severe disease progression and delay in recovery.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accessing the prevalence of cancer biomarkers in suspected patients from northeastern part of Bangladesh","authors":"M. Hasnat, M. Mia, Saifuddin Sarker, M. Chowdhury, M. Chowdhury, M. Hasan","doi":"10.5455/jabet.2023.d125","DOIUrl":"https://doi.org/10.5455/jabet.2023.d125","url":null,"abstract":"Background: Early detection and/or characterization of cancer associated biomarkers have revolutionized the personalized treatment approach of cancer to date. Therefore this study was design to accessed the prevalence of suspected cancer biomarkers from northeastern part of Bangladesh. Materials and Methods: A total of 892 patients’ data were analyzed in a cross-sectional manner and the collection period was 2017 to 2019. An ELISA-based chemiluminescent microparticle immune assay was used to detect serum cancer biomarkers. An unpaired t-test was used to compare the independent variables. Results: Data were clustered into four categories; group1 (10-17 yr, adolescent), group2 (18 to 35 yr, young adult), group3 (36 to 55 yr, middle), and group4 (>55 yr, older adult). Serum PSA level was higher in group4 than group3 (mean ± sem, 8.422 ± 1.423 vs 3.884 ±1.15 ng/ml). CA-125 is significantly higher in females with age > 35 yr (older and middle) than at age <35 yr (young adult and adolescent). No significant difference in AFP was found among the four age groups while sexual disparity has shown a statistically significant difference. Both males and females from group4 were showing a higher expression level of CEA (96.94 ± 54.02 and 48.23 ± 25.45 ng/ml respectively) though gender-wise test frequency was same. CA19-9 was high in group4 whereas no correlation was found between the gender variation. Conclusion: Our analysis have shown that level of PSA, CA-125, CEA and CA19-9 were upregulated in older peoples, while AFP was higher in male than female.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects of synbiotics on biofilm of uropathogenic Escherichia coli during urinary tract infection.","authors":"Mulia Pertiwi, M. Wahyunitisari, R. Setiabudi, A. Widodo, Nurul Wiqoyah, M. Arifijanto","doi":"10.5455/jabet.2023.d131","DOIUrl":"https://doi.org/10.5455/jabet.2023.d131","url":null,"abstract":"<jats:p />","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}