Pengfei Song, Jianfei Peng, Yan Liu, Chongzhi Zhong, Qiang Chen, Chao Zou, Shida Sun, Yajun Wu, Yao Gu, Taiyu Zhang, Ning Yang, Hongjun Mao
{"title":"Excess Vehicular Evaporative Emissions for Prolonged Parking during the COVID-19 Lockdown in China","authors":"Pengfei Song, Jianfei Peng, Yan Liu, Chongzhi Zhong, Qiang Chen, Chao Zou, Shida Sun, Yajun Wu, Yao Gu, Taiyu Zhang, Ning Yang, Hongjun Mao","doi":"10.1016/j.jhazmat.2025.139409","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139409","url":null,"abstract":"Vehicular evaporative emissions are major sources of urban volatile organic compounds (VOCs), significantly contributing to secondary organic aerosols (SOA) and ozone (O<sub>3</sub>) formation. While most anthropogenic emissions declined during the COVID-19 lockdowns, the potential rise in evaporative emissions has been largely overlooked. Here, we integrate emission tests, parking data, and field measurements to assess excess evaporative emissions in China. Long-term experiments reveal that prolonged parking vehicles (> 5 days) experience up to an 18-fold increase in evaporative emissions compared to the first day. During the lockdown, prolonged parking events surged dramatically, driving a substantial increase in evaporative emissions, strongly corroborated by VOC source apportionment analysis. We estimate that annual evaporative emissions across 36 Chinese megacities rose by up to 2.8-fold due to lockdown measures. These findings highlight prolonged parking as a critical yet underrecognized VOC source, emphasizing the need for improved regulatory control to mitigate urban air pollution.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"92 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shenxi Deng, Yi Yang, Erica Celeste Conway, Si Li, Cha Huang, Zigu Chen, Haisheng Wu, Yuan He, Shuzhe Xie, Aifang Ji, Jingyan Zhang, Yansheng Gu, Houyuan Lu, Changhong Lian, Jun Zheng, Linwei Tian, Qian Liu, Guibin Jiang
{"title":"Identification of the exposure pathway to micro-scaled biogenic silica debris via diet and its potential effect in promoting esophageal squamous cell carcinoma","authors":"Shenxi Deng, Yi Yang, Erica Celeste Conway, Si Li, Cha Huang, Zigu Chen, Haisheng Wu, Yuan He, Shuzhe Xie, Aifang Ji, Jingyan Zhang, Yansheng Gu, Houyuan Lu, Changhong Lian, Jun Zheng, Linwei Tian, Qian Liu, Guibin Jiang","doi":"10.1016/j.jhazmat.2025.139404","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139404","url":null,"abstract":"Esophageal squamous cell carcinoma (ESCC) has remained highly prevalent for decades in certain regions, such as the Taihang Mountains in China, though its etiology remains unclear. The previous study unveiled silica fragments in resected ESCC tissues in this region, raising a hypothesis about their relationship with ESCC. Yet, the exposure pathway of these silica fragments and their role in the high incidence of ESCC remain unclear. Here, we report micro-scaled needle-like biogenic silica debris (NL-BSDs) as contaminants in wheat flour, primarily originating from densest siliceous outgrowths on the lemma and palea of wheat husks. Simulating flour processing suggests that ineffective kernel-washing under water scarcity, a common climate feature for ESCC high-risk regions, allows more NL-BSDs to contaminate the flour. In addition, in an animal model, dietary exposure to NL-BSDs-bearing wheat husk potentially promotes tumorigenesis and induces inflammatory changes within esophagus and dysbiosis of gut microbiota, aligning with the classical two-stage tumorigenesis—initiation followed by promotion, suggesting that NL-BSDs contamination in diet could acts as a tumor promoter thus contributing to the high incidence of ESCC. Overall, our results suggest a previously neglected contamination in wheat flour and provide evidence for its adverse effects associated with esophageal tumors.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"24 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yunfei Jia, Bin He, Keyu Chen, Kuan Liu, Changjiang Yu, Bo Sun, Zhuo Ma, Bin Xu
{"title":"Excessive mitochondrial stress response triggers neuronal injury through the persistent eIF2α phosphorylation in mice exposed to manganese","authors":"Yunfei Jia, Bin He, Keyu Chen, Kuan Liu, Changjiang Yu, Bo Sun, Zhuo Ma, Bin Xu","doi":"10.1016/j.jhazmat.2025.139407","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139407","url":null,"abstract":"Manganese (Mn) overexposure induces neurocognitive abnormalities are intensively linked to hippocampal neuronal injury, but the neurotoxic mechanisms involved are ambiguous. Under various mitochondrial stress, mitochondrial stress response (MSR) is activated and plays a dual role in cells, promoting adaptive survival while also contributing to detrimental damage, depending on the severity of mitochondrial dysfunction. Excessive MSR can lead to inevitable cell death and organ damage, ultimately driving the onset and progression of numerous disorders. Yet, whether excessive MSR is implicated in Mn-induced neuronal injury remains unclear. In this study, Mn poisoning models is established in C57BL/6 mice and hippocampal primary neurons to investigate the role of excessive MSR in mitochondria-mediated neuronal apoptosis following Mn exposure. Specifically, excessive MSR triggered hippocampal neuronal mitochondrial damage and neurocognitive abnormalities, which is primarily driven by the persistent phosphorylation of eukaryotic translation initiation factor 2α (eIF2α). Furthermore, excessive acetylation of growth arrest and DNA damage-inducible protein 34 (GADD34) impairs the dephosphorylation of phospho-eIF2α by disrupting the protein phosphatase 1α/GADD34 complex in primary neurons following Mn exposure. Finally, Sirtuin 1-mediated GADD34 deacetylation facilitates the dephosphorylation of phospho-eIF2α, thus mitigating excessive MSR-triggered neuronal apoptosis and mitochondrial dysfunction. These findings underscore the critical role and complexity of excessive MSR in Mn-induced neuronal injury.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"1 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tianyi Zhao, Yuxuan Cao, Qinghe Cai, Yang Bai, Peng Huo, Lijia Zhang, Yang Zhang
{"title":"Multiple spectroscopic insights into the coupling effect of transition metals and water-soluble organic compounds on the oxidative potential of PM2.5 during haze and clean days","authors":"Tianyi Zhao, Yuxuan Cao, Qinghe Cai, Yang Bai, Peng Huo, Lijia Zhang, Yang Zhang","doi":"10.1016/j.jhazmat.2025.139406","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139406","url":null,"abstract":"Transition metals (TMs) and water-soluble organic compounds (WSOCs) are essential contributors to the oxidation potential (OP) of PM<sub>2.5</sub>. However, the interaction laws between WSOCs and TMs, as well as their coupling effect on OP in complex aerosol liquid phase still remain unclear, leading to huge uncertainties in assessing the health risks of PM<sub>2.5</sub>. In this study, the coupling effects of WSOCs and TMs on OP were assessed via a cell-free assay of 2’,7’-dichlorodihydrofluorescein (DCFH). The interaction laws between WSOCs and TMs were explored by excitation-emission matrix (EEM) fluorescence spectroscopy coupled with parallel factor analysis (PARAFAC) model. Results showed that WSOCs in haze days exhibited stronger OP (0.86±0.23<!-- --> <!-- -->nm m<sup>3</sup>) than that in clean days (0.31±0.16<!-- --> <!-- -->nm m<sup>3</sup>) due to higher proportion of water-soluble TMs. The complexation of TMs with WSOCs in haze days showed stronger ability to promote reactive oxygen species (ROS) generation than in clean days, and exhibited distinct time trends under different types of TMs and different pollution conditions. The proportion of highly oxygenated humic-like substances (C<sub>3</sub>) in WSOCs during haze days (38.39±0.49%) is higher than that during clean days (27.50±0.76%). Furthermore, the strongest complexing capacity between C<sub>3</sub> and specific TMs (Cu<sup>2+</sup> and V) forcefully explained the results of coupling effect. Our study revealed the complicated interaction laws of TMs and WSOCs under different atmospheric conditions and their potential effect on OP level in PM<sub>2.5</sub>. These findings are helpful to accurately assess the environmental behavior and health risks of PM<sub>2.5</sub>.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"25 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marija Opacic, Nemanja Todorovic, Mladena Lalic-Popovic, Bojana Stanic, Nebojsa Andric
{"title":"Advancing probabilistic risk assessment of perfluorooctanoic acid through integration of in vitro data and physiologically based toxicokinetic modeling coupled with population-specific analysis","authors":"Marija Opacic, Nemanja Todorovic, Mladena Lalic-Popovic, Bojana Stanic, Nebojsa Andric","doi":"10.1016/j.jhazmat.2025.139402","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139402","url":null,"abstract":"Current human health risk assessment for perfluorooctanoic acid (PFOA) has proven inadequate due to a lack of innovative approaches. Here, we applied benchmark dose-response modeling to derive benchmark concentrations (BMCs) for apical and transcriptomic changes in human endothelial cells EA.hy926 following both high-concentration short-term (48<!-- --> <!-- -->h) and human-relevant long-term (6 and 12 weeks) PFOA exposures. BMCs were translated into human equivalent doses (HEDs) using a physiologically based toxicokinetic model. Long-term exposure produced sensitive biological responses: 10<sup>th</sup> percentile HEDs for apical and transcriptomic data were 0.59 and 3.84<!-- --> <!-- -->ng/kg bw/week, respectively, both below the estimated U.S. general population exposure level (9.91<!-- --> <!-- -->ng/kg bw/week). Predicted 10<sup>th</sup> percentile HED thresholds in individuals with certain conditions were: end-stage renal disease 0.50<!-- --> <!-- -->ng/kg bw/week, moderate to severe reductions in glomerular filtration rate 0.18–1.00<!-- --> <!-- -->ng/kg bw/week, cirrhosis class A 0.18<!-- --> <!-- -->ng/kg bw/week, NAFLD 0.79<!-- --> <!-- -->ng/kg bw/week, and class 1 obesity 0.43<!-- --> <!-- -->ng/kg bw/week, compared to healthy individuals (1.18<!-- --> <!-- -->ng/kg bw/week), suggesting that PFOA can elicit adverse effects in endothelial cells at exposure levels below those currently experienced by the general population. Given the global prevalence of health conditions investigated in this study, a substantial portion of the population may face increased vulnerability to PFOA-induced health effects.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"26 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanduo Yang, Chenguang Hou, Jianchao Hao, Xueyong Zhou, Huifen Liu
{"title":"The correction of thermodynamic data published in Journal of Hazardous Materials 461 (2024) 132464","authors":"Yanduo Yang, Chenguang Hou, Jianchao Hao, Xueyong Zhou, Huifen Liu","doi":"10.1016/j.jhazmat.2025.139392","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139392","url":null,"abstract":"The dissemination of scientific knowledge depends on the ability of the authors, reviewers and publishers to provide available information and data to readers and mistakes, if not discovered, it will affect the academic quality of the paper. Recently, Liu et al. published an important academic paper that investigated the adsorption behavior of thiram by four common types of microplastics (MPs) and its hazardous change after that adsorption. However, the thermodynamic parameters of Δ<em>G</em>, Δ<em>H</em>, and Δ<em>S</em> need to be corrected because their data in <strong>Table 4</strong> did not support their thermodynamic conclusions. In the present study, the value of Δ<em>G</em> was confirmed based on the thermodynamic calculation principle and the description of the thermodynamic results by Liu et al., then the values of Δ<em>H</em>, and Δ<em>S</em> were recalculated using the confirmed Δ<em>G</em> values. After the above corrections, the contradiction between the data and the conclusion has been resolved. In addition, the present study provided a method for calculating Δ<em>G</em><sup>o</sup> using the standard equilibrium constant <em>K</em><sup>o</sup>, thereby eliminating the errors caused by non-standard equilibrium constant<em>.</em> There is a growing need to enhance scientific rigor across many fields of scientific research. Rigorous scientific papers need to demonstrate creative experimental designs, but also provide precise results. Here, we just do that.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"11 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wei-Zhao Yin, Jin-Xiang Xu, Jin Xu, Gang Lu, Hai Liu
{"title":"Synchronous nanowire-assisted electroporation and peracetic acid oxidation to inhibit VBNC cells formation: Reversible electroporation pores reinforce permeation of peracetic acid for cellular destruction","authors":"Wei-Zhao Yin, Jin-Xiang Xu, Jin Xu, Gang Lu, Hai Liu","doi":"10.1016/j.jhazmat.2025.139405","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139405","url":null,"abstract":"The occurrence of viable but non-culturable (VBNC) bacteria may dramatically underestimate microorganic risks in drinking water. Herein, peracetic acid (PAA) oxidation, a green and potent disinfection method, was combined with a physical nanowire-assisted electroporation (EP) to develop the synchronous EP/PAA approach, aiming to reinforce bacterial inactivation via facilitating PAA permeation from EP-induced pores for oxidative damage of cell structures. The locally enhanced electric field near nanowire tips at a low voltage of 1.5<!-- --> <!-- -->V induced 38.4% and 52.4% cells with reversible and irreversible membrane damages, accompanied by 72.5% cells inactivation and 18.3% VBNC cells formation. The EP/PAA exhibited significant synergistic effects on removal of viable <em>E. coli</em> (G-), <em>P. aeruginosa</em> (G-), <em>E. faecalis</em> (G+), and <em>S. aureus</em> (G+) cells, especially achieving above 5-log removal (99.999%) of these viable cells with ~4-log synergistic effects and ~5–9 times less energy consumption than the individual EP and PAA (~40%–80%). Characterizations of cell membrane integrity and cellular morphology revealed that EP-induced membrane pores facilitated PAA permeation to destruct cell structures, thereby minimizing the risks of VBNC cell formation and resuscitation. The excellent synergistic effects and long-term stability of EP/PAA in tap water demonstrated its promising application potential to control VBNC cells in drinking water.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"144 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The miR-122/P53/TIGAR signaling axis regulates glucose metabolic reprogramming induced by polystyrene nanoplastic particles","authors":"Ting Chen, Changyong Gong, Fan Yu, Helei Cai, Xinxin Yao, Shihua Chen, Qiufang Wu, Yize Wu, Wenhui Xu, Feiqin Xie, Libo Jin, Renyi Peng","doi":"10.1016/j.jhazmat.2025.139408","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139408","url":null,"abstract":"Polystyrene nanoplastics (PS-NPs) are widespread environmental pollutants that cause toxicity, including metabolic disruption. However, their exact molecular mechanisms remain unclear. This study systematically investigated the effects of PS-NPs on glucose metabolism using both in vivo and in vitro models, employing zebrafish larvae and Hep G2 cell lines. It was found that exposure to PS-NPs induced oxidative stress and liver damage in zebrafish larvae, while the liver-specific expression of <em>miR-122</em> was significantly downregulated, leading to the upregulation of its target gene <em>P53</em> and its downstream effector <em>TIGAR</em>. Combined metabolomics and transcriptomics analysis showed that PS-NPs exposure caused glucose metabolism disorders, with P53 and its downstream effector TIGAR playing a key regulatory role in the glycolytic pathway (EMP) and the pentose phosphate pathway (PPP). Through miR-122-5p mimic, miR-122-5p inhibitor, P53 RNA interference, and co-transfection experiments, we further demonstrated that glucose metabolism could be driven from the EMP shifted to the PPP through the miR-122/P53/TIGAR axis. This study, for the first time, reveals the molecular mechanism by which PS-NPs regulate glucose metabolism via the miR-122/P53/TIGAR signaling axis. It provides a novel insight for understanding the impact of miR-122-mediated glucose metabolic reprogramming, and enhances the understanding of miRNA function in response to NPs exposure.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"1 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Perfluorobutanesulfonic acid (PFBS) on the sediment microbial communities, and the interaction mechanism with urease and extracellular polymeric substances (EPS)","authors":"Yaxuan Wu, Bowen Yang, Qianzhi Zeng, Yunhong Pu, Qing Yang, Yiming Hao, Yue Sun, Yulian Ma, Xinyu Yang, Xin Wang, Shengnan Shi, Zheng Gong","doi":"10.1016/j.jhazmat.2025.139396","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139396","url":null,"abstract":"The influence of perfluorobutane sulphonic acid (PFBS) on sediment microbial communities and nitrogen cycling was systematically investigated in this study. After 28 d of PFBS, the diversity of sediment microorganisms was inhibited, with rare bacterial community demonstrating stronger structural decisive role than abundant taxa. Meanwhile, PFBS induced the secretion of extracellular polymeric substances (EPS), causing elevated reactive oxygen species levels and triggering antioxidant defences. EPS could also act as a barrier between PFBS and urease to reduce the toxicity of PFBS to urease. In the PFBS-urease-EPS system, ultraviolet-visible spectroscopy revealed intensified absorption peaks corresponding to π→π* transitions in peptide backbone structures, indicating that there was an interaction between urease and EPS, a change in the structure of the urease backbone. And the increase in α-helix content indicated that the secondary structure of urease becomes more compact and improves urease activity, which subsequently facilitated ammonium nitrogen (NH<sub>4</sub><sup>+</sup>-N) production in sediments. Partial Least Squares Path Models (PLS-PM) indicated that PFBS stress directly altered the abundance of nitrogen-cycling bacteria, upregulated genes encoding enzymes, and increased the activities of key enzymes of nitrogen-cycling processes, thereby promoting nitrogen cycling.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"16 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Umer Asghar, Yuting Zhai, Ting Liu, Peixin Fan, Noor Ul Ain, Arsalan Haseeb Zaidi, Muhammad Tariq, Raul C. Mainar-Jaime, Kwangcheol C. Jeong
{"title":"A Metagenomics-Based Approach to Understanding the Transmission of Healthcare-Associated Antimicrobial Resistance in Pakistan","authors":"Muhammad Umer Asghar, Yuting Zhai, Ting Liu, Peixin Fan, Noor Ul Ain, Arsalan Haseeb Zaidi, Muhammad Tariq, Raul C. Mainar-Jaime, Kwangcheol C. Jeong","doi":"10.1016/j.jhazmat.2025.139384","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.139384","url":null,"abstract":"Hospital environments are critical yet underexamined reservoirs for hazardous antimicrobial resistance (AMR), particularly in lower-middle-income countries (LMICs) where resource constraints often hinder comprehensive surveillance. In this study, we employed 16S rRNA gene sequencing and shotgun metagenomics to characterize the microbiome, resistome, and potential transmission routes across five clinical environments within a hospital in Pakistan: the intensive care unit (ICU), surgical ward (SW), cardiac surgery ward (CSW), cardiac ward (CW), and operating theater (OT). Microbial community analysis revealed compositional similarities among the ICU, SW, and OT, with the ICU emerging as a primary source of microbial dissemination. Species-level profiling identified hospital-associated pathogens such as <em>Acinetobacter baumannii</em>, <em>Klebsiella pneumoniae</em>, and <em>Enterobacter cloacae,</em> and metagenome-assembled genome (MAG) analysis enabled the linkage of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) to specific bacterial hosts. ARGs and MGEs displayed setting-specific patterns, with the SW harboring the highest abundance of ARGs, particularly those conferring resistance to β-lactams and biocides. Insertion sequences were the most prevalent MGEs and were commonly linked to ARGs, indicating potential horizontal gene transfer. Co-occurrence network analysis identified <em>Staphylococcus</em>, <em>Enterococcus</em>, and <em>Escherichia</em> as central hub taxa within the microbial communities of the ICU, SW, and OT, indicating their critical roles in potential ARG transmission. These findings provide critical insights into the environmental transmission dynamics of AMR in LMIC healthcare settings and underscore the urgent need for metagenomics-informed infection control strategies.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"717 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}