Journal of Hazardous Materials最新文献

{"title":"Machine learning-accelerated determination of immunotoxicities of liquid crystal monomers","authors":"Jiawei Cheng, Yawen Yuan, Yunsong Mu, Fengchang Wu, Hyeong-Moo Shin, Christie M. Sayes, John P. Giesy","doi":"10.1016/j.jhazmat.2025.140166","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140166","url":null,"abstract":"Liquid crystal monomers (LCMs), essential components of liquid crystal displays (LCDs), have emerged as a growing global concern because of their potential adverse effects on human health. However, experimental evidence elucidating their toxicological mechanisms remains scarce. In this study, a novel mode-of-action (MOA)-based graph neural network (GNN) framework was introduced to (1) identify the molecular initiating event (MIE) through which LCMs disrupt immune homeostasis, (2) predict their binding affinity to 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1), and (3) identify toxicity-relevant substructures to guide the molecular design of safer LCMs. First, an unsupervised clustering approach was applied to categorize over 1,400 LCMs into four structural clusters. Then, two virtual screening methods (<em>i.e.</em>, 2D/3D structural similarity analysis and a pharmacophore-based model) were applied to identify 121 possible receptors that could interact by potential insertions of LCM with a total universe of approximately 2,000 human protein receptors. Through “fingerprint”-based, t-distributed stochastic neighbor embedding (t-SNE) and molecular similarity analysis using PubChem, LCMs were found to be potential inhibitors of HSD11B1. Third, docking simulations revealed that highly fluorinated and ester-containing monomers exhibit stronger binding affinities at the HSD11B1 active site, primarily through stable hydrogen bonding. Finally, a GNN model was developed, which accurately predicted LCM-HSD11B1 binding affinities (<em>R</em>² = 0.90, <em>RMSE</em> = 2.36) and identified key structural features contributing to immunotoxicity. Furthermore, a user-friendly, web-based prediction tool was developed to facilitate broader applications. These findings reveal HSD11B1 as a novel MIE of LCM-induced immunotoxicity and provide a practical immunotoxicity prediction platform to support risk assessment and the design of environmentally friendly LCM alternatives in LCD manufacturing.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"2 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145314820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental UV Filters Threaten Human Neurodevelopment: Disruption of Central and Peripheral Nervous System Lineage Commitment via WNT Signaling Dysregulation","authors":"Shichang Li, Shuxian Zhang, Hanyue Li, Nuoya Yin, Renjun Yang, Yang Zhang, Magdalena J Koziol, Francesco Faiola","doi":"10.1016/j.jhazmat.2025.140149","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140149","url":null,"abstract":"Ultraviolet filters (UVFs) are extensively employed in personal care products, coatings, and food packaging materials, raising increasing concerns regarding developmental vulnerability due to their frequent detection in breast milk and amniotic fluid. This study systematically evaluated the developmental neurotoxicity of representative benzophenone, benzotriazole, and triazine UVFs using human embryonic stem cell (hESC) differentiation models. The dynamic trajectories of both central and peripheral nervous system (CNS and PNS) development were tracked, revealing that UVFs induced an imbalanced CNS/PNS lineage commitment, with UV-328 particularly causing disruptions through direct modulation of the WNT signaling pathway. In the PNS, 10<!-- --> <!-- -->nM UV-328 significantly inhibited proliferation and migration in neural crest cells by preventing E-cadherin downregulation. Conversely, in the CNS, BP-3, UV-328, and BEMT promoted aberrant midbrain differentiation by upregulating <em>FOXA2, LMX1A, CORIN, OTX2,</em> and <em>EN1</em> expression, while impairing late-stage dopaminergic neuron specification by downregulating TH. These findings elucidate the molecular mechanisms underlying benzophenone, benzotriazole, and triazine UVF-induced developmental neurotoxicity and underscore the critical need to evaluate the environmental impact and safety of UVFs during sensitive developmental stages. Additionally, this study highlights the potential links between UVF exposure and neurological disorders, emphasizing the importance of continued investigation in this field to inform environmental policies and public health strategies.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"27 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Insight into the Mechanism of DEHP-Induced Cardiotoxicity: Triggering pyroptosis of cardiomyocytes by disrupting sphingolipid metabolism","authors":"Jia-Gen Cui, Hao Zhang, Yue-Qi Zhang, Bing Sun, Xue-Nan Li, Yi Zhao, Jin-Long Li","doi":"10.1016/j.jhazmat.2025.140148","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140148","url":null,"abstract":"The widespread use of di(2-ethylhexyl) phthalate (DEHP) as a plasticizer raises concerns due to its environmental persistence and cardiotoxicity. Nevertheless, the precise mechanisms through which DEHP induces cardiac damage, particularly concerning myocardial injury, remain inadequately understood. Using a mouse model, this investigation employed UPLC-MS/MS to demonstrate that exposure to DEHP significantly elevated the concentration of its primary metabolite, mono(2-ethylhexyl) phthalate (MEHP), in cardiac tissue, while DEHP itself did not exhibit a significant difference. DEHP-induced myocardial injury in mice was evidenced by disorganized myocardial fibers, collagen deposition and elevated serum creatine kinase-MB (CK-MB) and C-reactive protein (CRP). Further analysis through database screening and experimental validation indicated that DEHP mediates myocardial injury by activating the sphingolipid metabolic pathway involving the sphingosine kinases 1 (SPHK1)/ sphingosine 1-phosphate receptor 2 (S1PR2) axis. Molecular docking and cellular thermal shift assays (CETSA) confirmed that MEHP could directly bind to and enhance the stability of SPHK1. DEHP exposure also increased pyroptosis-related proteins, reversible via SPHK1 inhibitor PF543 or S1PR2 siRNA. In conclusion, this study first links DEHP-induced cardiac pyroptosis to SPHK1/S1PR2 signaling in mice, providing novel insights into DEHP-associated cardiotoxicity, contributing to cardiac damage and thereby offering new theoretical insights into DEHP-related cardiac toxicity.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"171 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geometrically-Driven Selective Transport of Microplastics Across Atmosphere-Land","authors":"Shuangqi Wu, Mengrong Bao, Weicong Wang, Jianshi Huang, Chenglin Cai, Jiawei Xie, Lei Zhou, Liming Zhang, Ying Wang, Shuiping Cheng","doi":"10.1016/j.jhazmat.2025.140175","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140175","url":null,"abstract":"Atmospheric circulation contributes critically to the transport of microplastics (MPs). However, our limited knowledge regarding the geometric characteristics of deposited MPs hinders the accurate assessment of associated environmental risks. This study explores how the geometrical characteristics influence MPs' selective atmospheric transport and deposition in Shanghai, which highlights MP geometric characteristics as a crucial indicator for understanding atmospheric-terrestrial transport mechanisms. High-density deposited MPs exhibited heterogeneous geometric characteristics, and low-density MPs showed partially homogenized characteristics. Meteorological parameters correlate more significantly with geometric characteristics than with deposition flux in our research. Precipitation significantly promotes deposited MPs characterized by high solidity, high circularity, and low density. Moreover, increased wind speeds driven by atmospheric pressure gradients significantly heightened the deposition of MPs with high solidity. Typhoon events enhance MP deposition by promoting the resuspension of terrestrial MPs and the release of MPs from damaged urban infrastructure. Potential source contribution function (PSCF) analysis indicates that both local and remote transport pathways contribute to MP deposition in Shanghai. These results provide critical insights for refining MP transport models by incorporating geometric features, elucidating the environmental fate of MP, and supporting the development of targeted strategies to mitigate human exposure risks.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"53 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field Demonstration of In Situ Stabilization (ISS) of Per- and Polyfluoroalkyl Substances in Soil with RemBind®","authors":"Craig Divine, Lindsey Melicharek, David Gomes, Kim Heinze, Jay Erickson, Brent Barker, David Liles, Andy Baumeister, Eric Foote, Jeffery Aust, Tyler Dugan, McCullough Wells, Sean Bayer","doi":"10.1016/j.jhazmat.2025.140127","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140127","url":null,"abstract":"A field study was completed to assess the effectiveness of in-situ soil stabilization (ISS) to immobilize per- and polyfluoroalkyl substances (PFAS) impacted soil, including perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), in a former burn pit at the Cavalier Space Force Station, North Dakota. Only a few field ISS studies have been published to-date and this work provides important practical considerations and performance data. Based on the results of a laboratory treatability study, RemBind® 100X, which consists of an aluminum hydroxide/carbon blend (amorphous aluminum hydroxide, kaolinite clay, and activated carbon), was applied at 3 percent by dry weight as a stabilizing material (or fixant) to impacted soil to reduce PFAS leaching from soil to groundwater. The fixant was distributed and mixed over a 3-foot vertical zone across a 100- x 100-foot area gridded into 18 test cells. Four performance monitoring events were completed throughout 19 months, during which soil samples were collected to assess PFAS leaching using Leaching Environmental Assessment Framework (LEAF) analysis, and vadose zone moisture was monitored using downhole nuclear magnetic resonance (NMR) imaging and in-situ moisture probes. For all PFAS analyzed, leachate concentrations following leachate testing, were reduced by at least 95 percent (±5.1%) for each of the four sampling events and concentrations were measured at levels less than the United States Environmental Protection Agency’s (USEPA’s) regional screening levels (OSD 2023) and maximum contaminant levels (EPA 2024) for PFOS, PFOA, perfluorobutanoic acid (PFBA), perfluorobutanesulfonic acid (PFBS), perfluorononanoic acid (PFNA), perfluorohexanoic acid (PFHxA), perfluorohexanesulfonic acid (PFHxS), and hexafluoropropylene oxide dimer acid (HFPO-DA). Furthermore, the approach was effective at reducing leaching from the stabilized soils, while still allowing infiltration of precipitation. Overall, this study confirms the efficacy of this technology and therefore should be consideration at other similar PFAS-impacted sites.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"66 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution of Rubber Modified Asphalt to Emission and Odors Tracing: Experimental and Modelling Investigation","authors":"Lingwen Li, Tao Zhou, Liping Cao, Hu Yang, Ajit K Sarmah, Zejiao Dong, Twagirimana Emmanuel, Qilin Huang","doi":"10.1016/j.jhazmat.2025.140174","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140174","url":null,"abstract":"Rubber modified asphalt (RMA) is widely used in infrastructure due to its superior performance; however, its emissions pose environmental and health risks, particularly in enclosed or densely populated areas. This study used emission kinetics, odor assessment, and health risk analysis methods to evaluate the emission characteristics and risks assessment of different types of RMA and its extracted crumb rubber, and reveal the contributions of crumb rubber to RMA emissions. Results revealed that for RMA, macroscopic emission kinetics of H₂S and volatile organic compounds (VOCs) emissions from RMA followed a bi-exponential function, with odor primarily driven by cyclic aromatic hydrocarbons and hydrocarbon derivatives, accounting for &gt;99%. Key odorants identified were octanal, benzothiazole, and 4-methyl-dibenzothiophene. 4-methyl-dibenzothiophene has been identified as a key target for controlling odor and health risks throughout the entire RMA lifecycle. Benzothiazole, decanal, and octanal are found to be characteristic odor contributors of crumb rubber to RMA. Odor assessment analysis confirmed crumb rubber was the major VOCs source in early emission stages. This study provides data-driven guidance for tracking odor emission sources, developing low-emission RMA materials, supporting sustainable infrastructure practices, and improving occupational health standards.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"2 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Source-specific fingerprints and machine learning-driven apportionment of lead-containing fine particles from typical industrial emissions in China","authors":"Xuanhe Zhao, Zuoshun Niu, Miao Xu, Lingyan Wu, Mengyuan Wang, Zhiqiang Shi, Yujie Cui, Jing Chen, Yi Yang","doi":"10.1016/j.jhazmat.2025.140173","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140173","url":null,"abstract":"Lead-containing fine particles (Pb-FPs) from industrial emissions pose significant health risks, but their source-specific characteristics remain poorly characterized. This study presents a comprehensive investigation of Pb-FPs derived from four major industrial sectors in China, i.e. coal-fired power (CFP), iron and steel smelting (ISS), waste incineration power (WIP), and biomass power generation (BP), through systematic analysis of 134 PM samples collected nationwide using single-particle inductively coupled plasma time-of-flight mass spectrometry (spICP-TOF-MS). Our results showed that WIP (5×10⁷ particles/mg) and ISS (3.9×10⁷ particles/mg) activities emitted significantly higher number concentrations of Pb-FPs compared to CFP and BP sources.Pb-multi-metal FPs accounted for 66.7-81.2% of total Pb-FPs number concentrations across all sources, with the mass fraction of Pb was predominantly ≤ 10%. Distinct elemental fingerprints were identified for each source type, particularly metal-rich matrices associated with Pb. We developed a source apportionment approach by evaluating five machine learning algorithms, with XGBoost emerging as the optimal classifier (F1 score = 0.76, accuracy = 0.77) after Bayesian optimization and 10-fold cross-validation. Application of the model to PM_2.5 samples from Beijing and Shanghai revealed persistent and substantial contributions from ISS-derived Pb-FPs (6.7-38.1% in Beijing, 10.5-33.7% in Shanghai), with additional average inputs from CFP (7.4%), WIP (5.8%), and BP (12.1%). These results highlight the dominant role of ISS in atmospheric Pb pollution across industrialized regions of China and emphasize the need for targeted mitigation strategies.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"11 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VOC Emissions Characteristics and Cooperative Control by Combustion Adjustment and APCDs in Supercritical Coal-Fired Power Plants","authors":"Penghui Ge, Jie Wu, Longchun Zhong, Wei-Ping Pan","doi":"10.1016/j.jhazmat.2025.140177","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140177","url":null,"abstract":"This study examined volatile organic compound (VOC) emission characteristics in three supercritical coal-fired power plants (660<!-- --> <!-- -->MW, 600<!-- --> <!-- -->MW, and 330<!-- --> <!-- -->MW units), with five tasks: the effect of load, excess air ratio, combustion flame location (adjust the burners), air pollution control device (APCD) operational scenarios, and co-firing with biomass (10%) and sludge (10%), all of which were carried out in situ. Each task included two similar setups on the boiler and APCDs for comparison. A 1.4<!-- --> <!-- -->MW pilot scale was included to provide the most challenging tasks, which were difficult to conduct in the power plant. Around 60 to 70% of VOC was reduced by optimizing the boiler combustion conditions, which was most influenced by increasing the flame center height and adjusting the secondary air ratio. The rest (30-40%) of the VOC entered the APCD's system from the boiler, and the central portion of the VOC in the tail gas was effectively oxidized through the catalytic oxidation process in the SCR system. The ESP had negligible removal effects on VOC, and the high-voltage discharge enhanced the re-emission of VOCs from fly ash. Thus, combustion condition optimizations combined with APCDs could synergistically remove over 90% of VOCs in most power plants. In addition, the blending of biomass and sludge will increase the production of VOCs. The study is crucial for achieving clean coal power production and significantly reducing VOC emissions.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"198 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient removal of polystyrene nanoplastics from complex water system through multiple driving forces with MOF-based composite","authors":"Shiqian Zhou, Xiao Wang, Xujing Chen, Shijie Yang, Kai Chen, Guanqing Song, Guanhong Lu, Yan Wang, Jing Sun","doi":"10.1016/j.jhazmat.2025.140171","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140171","url":null,"abstract":"As emerging contaminants widely present in aquatic environments, micro/nano plastics (MPs/NPs) pose global environmental and biosafety concerns. The efficient removal of MPs/NPs faces challenges such as poor selectivity and low cyclic stability of traditional adsorbents. This study developed an in-situ synthesis strategy to integrate UIO-66 with melamine foam (MF), successfully constructing UIO-66/MF (UMF) composite that combined high adsorption capacity with excellent structural stability. The results showed that UMF achieved adsorption equilibrium for PS NPs within 300<!-- --> <!-- -->min, with an adsorption capacity of 65.5<!-- --> <!-- -->mg/g. Moreover, UMF maintained over 93% and 82% adsorption efficiency in a broad pH range (3-10) and under complex aqueous conditions, respectively. Mechanistic analysis revealed that multiple synergistic interactions were involved in the adsorption process, including hydrophobic interactions, electrostatic interactions, π-π stacking, hydrogen bonding and cation-π interactions. Furthermore, we systematically disclosed the factors which led to the decreased adsorption efficiency in tap water and river water. Notably, UMF exhibited exceptional reusability, retaining higher than 81% removal efficiency after 25 cycles. UMF effectively addresses the challenges of MOFs powders instability and recycling difficulties, achieving a balance between economy, sustainability, and practicality. This composite provides a promising solution for remediating MPs/NPs pollution in aqueous environments.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"58 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic mineralization of the UV filter benzophenone-3 by a cross-feeding consortium from wastewater treatment plants: Insights into novel pathway and bioremediation strategy","authors":"Ao Zhang, Piaopiao Pan, Ning-Yi Zhou, Tao Li","doi":"10.1016/j.jhazmat.2025.140176","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.140176","url":null,"abstract":"Benzophenone-3 (BP-3), used as an organic UV filter in diverse consumer products including cosmetics, has been frequently detected in wastewater treatment plants (WWTPs) and aquatic environments. BP-3 and its transformation products are regarded as emerging micropollutants due to their low biodegradability. Here, we investigate the synergistic degradation of BP-3 by a bacterial consortium seeded from aerobic sludge WWTPs. BP-3 is found to be initially degraded through a novel pathway involving a C-C bond cleavage step, producing intermediates 3-methoxyphenol (3MOP) and benzoate, two naturally occurring compounds which can be readily degraded in the environment. Metagenome-guided pure culture isolation and pathway analysis reveal that bacterial strains from genera <em>Pigmentiphaga</em> and <em>Brucella</em> synergistically contribute to the BP-3 mineralization. Specifically, the <em>Pigmentiphaga</em> strain degrades BP-3 into benzoate and 3MOP, with the former being utilized by itself and the latter utilized by the <em>Brucella</em> strain. A reconstructed consortium, consisting of two isolated strains from <em>Pigmentiphaga</em> and <em>Brucella</em>, exhibits similar degradation performance to that of the natural consortium, indicating their crucial roles in environmental BP-3 degradation. These findings provide new insights into BP-3 biodegradation at the microbial community level, offering potential strategies for wastewater treatment applications by manipulating synthetic microbial consortia.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"5 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}