Cells and Development最新文献

{"title":"Xenopus tropicalis osteoblast-specific open chromatin regions reveal promoters and enhancers involved in human skeletal phenotypes and shed light on early vertebrate evolution","authors":"","doi":"10.1016/j.cdev.2024.203924","DOIUrl":"10.1016/j.cdev.2024.203924","url":null,"abstract":"<div><p><span>While understanding the genetic underpinnings of osteogenesis has far-reaching implications for skeletal diseases and evolution, a comprehensive characterization of the osteoblastic regulatory landscape in non-mammalian vertebrates is still lacking. Here, we compared the ATAC-Seq profile of </span><span><em>Xenopus tropicalis</em></span> (<em>Xt</em><span>) osteoblasts to a variety of non mineralizing control tissues, and identified osteoblast-specific nucleosome free regions (NFRs) at 527 promoters and 6747 distal regions. Sequence analyses<span>, Gene Ontology<span>, RNA-Seq and ChIP-Seq against four key histone marks confirmed that the distal regions correspond to </span></span></span><em>bona fide</em> osteogenic transcriptional enhancers exhibiting a shared regulatory logic with mammals. We report 425 regulatory regions conserved with human and globally associated to skeletogenic genes. Of these, 35 regions have been shown to impact human skeletal phenotypes by GWAS, including one <em>trps1</em> enhancer and the <em>runx2</em><span> promoter, two genes which are respectively involved in trichorhinophalangeal syndrome type I and cleidocranial dysplasia. Intriguingly, 60 osteoblastic NFRs also align to the genome of the elephant shark, a species lacking osteoblasts and bone tissue. To tackle this paradox, we chose to focus on </span><em>dlx5</em> because its conserved promoter, known to integrate regulatory inputs during mammalian osteogenesis, harbours an osteoblast-specific NFR in both frog and human. Hence, we show that <em>dlx5</em> is expressed in <em>Xt</em> and elephant shark odontoblasts, supporting a common cellular and genetic origin of bone and dentine. Taken together, our work (i) unravels the <em>Xt</em> osteogenic regulatory landscape, (ii) illustrates how cross-species comparisons harvest data relevant to human biology and (iii) reveals that a set of genes including <em>bnc2</em>, <em>dlx5</em>, <em>ebf3</em>, <em>mir199a</em>, <em>nfia</em>, <em>runx2</em> and <em>zfhx4</em> drove the development of a primitive form of mineralized skeletal tissue deep in the vertebrate lineage.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"179 ","pages":"Article 203924"},"PeriodicalIF":3.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive patterning of vascular network during avian skin development: Mesenchymal plasticity and dermal vasculogenesis","authors":"","doi":"10.1016/j.cdev.2024.203922","DOIUrl":"10.1016/j.cdev.2024.203922","url":null,"abstract":"<div><p><span><span>A vasculature<span> network supplies blood to feather buds in the developing skin. Does the vasculature network during early skin development form by sequential sprouting from the central vasculature or does local vasculogenesis<span><span> occur first that then connect with the central vascular tree<span><span>? Using transgenic<span> Japanese quail Tg(TIE1p.H2B-eYFP), we observe that vascular </span></span>progenitor cells<span> appear after feather primordia formation. The vasculature then radiates out from each bud and connects with primordial vessels from neighboring buds. Later they connect with the central vasculature. Epithelial-mesenchymal recombination shows local vasculature is patterned by the epithelium, which expresses </span></span></span>FGF2 and VEGF. Perturbing noggin expression leads to abnormal vascularization. To study endothelial origin, we compare </span></span></span>transcriptomes of TIE1p.H2B-eYFP</span>⁺<span><span> cells collected from the skin and aorta. Endothelial cells from the skin more closely resemble skin dermal cells than those from the aorta. The results show developing chicken skin vasculature is assembled by (1) physiological </span>vasculogenesis from the peripheral tissue, and (2) subsequently connects with the central vasculature. The work implies mesenchymal plasticity and convergent differentiation play significant roles in development, and such processes may be re-activated during adult regeneration.</span></p></div><div><h3>Summary statement</h3><p>We show the vasculature network in the chicken skin is assembled using existing feather buds as the template, and endothelia are derived from local bud dermis and central vasculature.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"179 ","pages":"Article 203922"},"PeriodicalIF":3.9,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen IV assembly is influenced by fluid flow in kidney cell-derived matrices","authors":"","doi":"10.1016/j.cdev.2024.203923","DOIUrl":"10.1016/j.cdev.2024.203923","url":null,"abstract":"<div><p>Kidney podocytes and endothelial cells assemble a complex and dynamic basement membrane that is essential for kidney filtration. Whilst many components of this specialised matrix are known, the influence of fluid flow on its assembly and organisation remains poorly understood. Using the coculture of podocytes and glomerular endothelial cells in a low-shear stress, high-flow bioreactor, we investigated the effect of laminar fluid flow on the composition and assembly of cell-derived matrix. With immunofluorescence and matrix image analysis we found flow-mediated remodelling of collagen IV. Using proteomic analysis of the cell-derived matrix we identified changes in both abundance and composition of matrix proteins under flow, including the collagen-modifying enzyme, prolyl 4-hydroxylase (P4HA1). To track collagen IV assembly, we used CRISPR-Cas9 to knock in the luminescent marker HiBiT to the endogenous <em>COL4A2</em> gene in podocytes. With this system, we found that collagen IV was secreted and accumulated consistently under both static and flow conditions. However knockdown of <em>P4HA1</em> in podocytes led to a reduction in the secretion of collagen IV and this was more pronounced under flow. Together, this work demonstrates the effect of fluid flow on the composition, modification, and organisation of kidney cell-derived matrix and provides an <em>in vitro</em> system for investigating flow-induced matrix alteration in the context of kidney development and disease.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"179 ","pages":"Article 203923"},"PeriodicalIF":3.9,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266729012400024X/pdfft?md5=31aac5f90f4486208605930cc23b13db&pid=1-s2.0-S266729012400024X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140756909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain enlargement with rostral bias in larvae from a spontaneously occurring female variant line of Xenopus; role of aberrant embryonic Wnt/β-catenin signaling","authors":"","doi":"10.1016/j.cdev.2024.203918","DOIUrl":"10.1016/j.cdev.2024.203918","url":null,"abstract":"<div><p>Increased brain size and its rostral bias are hallmarks of vertebrate evolution, but the underlying developmental and genetic basis remains poorly understood. To provide clues to understanding vertebrate brain evolution, we investigated the developmental mechanisms of brain enlargement observed in the offspring of a previously unrecognized, spontaneously occurring female variant line of <em>Xenopus</em> that appears to reflect a genetic variation. Brain enlargement in larvae from this line showed a pronounced rostral bias that could be traced back to the neural plate, the primordium of the brain. At the gastrula stage, the Spemann organizer, which is known to induce the neural plate from the adjacent dorsal ectoderm and give it the initial rostrocaudal patterning, was expanded from dorsal to ventral in a large proportion of the offspring of variant females. Consistently, <em>siamois</em> expression, which is required for Spemann organizer formation, was expanded laterally from dorsal to ventral at the blastula stage in variant offspring. This implies that the active region of the Wnt/β-catenin signaling pathway was similarly expanded in advance on the dorsal side, as <em>siamois</em> is a target gene of this pathway. Notably, the earliest detectable change in variant offspring was in fertilized eggs, in which maternal <em>wnt11b</em> mRNA, a candidate dorsalizing factor responsible for activating Wnt/β-catenin signaling in the dorsal embryonic region, had a wider distribution in the vegetal cortical cytoplasm. Since lateral spreading of <em>wnt11b</em> mRNA, and possibly that of other potential maternal dorsalizing factors in these eggs, is expected to facilitate lateral expansion of the active region of the Wnt/β-catenin pathway during subsequent embryonic stages, we concluded that aberrant Wnt/β-catenin signaling could cause rostral-biased brain enlargement via expansion of <em>siamois</em> expression and consequent expansion of the Spemann organizer in <em>Xenopus</em>. Our studies of spontaneously occurring variations in brain development in <em>Xenopus</em> would provide hints for uncovering genetic mutations that drive analogous morphogenetic variations during vertebrate brain evolution.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"179 ","pages":"Article 203918"},"PeriodicalIF":3.9,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667290124000196/pdfft?md5=50ad07a003ccba433f21276ba4595e59&pid=1-s2.0-S2667290124000196-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifications of three novel alleles of Serrate in Drosophila","authors":"Qinghai Zhang ,&nbsp;Pei Zhang ,&nbsp;Min Yang ,&nbsp;Yingxue Tian ,&nbsp;Chunxia Feng ,&nbsp;Wei Wei","doi":"10.1016/j.cdev.2024.203908","DOIUrl":"10.1016/j.cdev.2024.203908","url":null,"abstract":"<div><p>The Notch signaling pathway, an evolutionarily highly conserved pathway, participates in various essential physiological processes in organisms. Activation of Notch signaling in the canonical manner requires the combination of ligand and receptor. There are two ligands of Notch in <em>Drosophila</em>: Delta (Dl) and Serrate (Ser). A mutation <em>mf157</em> is identified for causing nicks of fly wings in genetic analysis from a mutant library (unpublished) that was established previously. Immunofluorescent staining illustrates that <em>mf157</em> represses the expression of Cut and Wingless (Wg), the targets of Notch signaling. MARCM cloning analysis reveals that <em>mf157</em> functions at the same level or the upstream of ligands of Notch in signaling sending cells. Sequencing demonstrates that <em>mf157</em> is a novel allele of the <em>Ser</em> gene. Subsequently, <em>mf553</em> and <em>mf167</em> are also identified as new alleles of <em>Ser</em> from our library. Furthermore, the complementary assays and the examination of transcripts confirm the sequencing results. Besides, the repressed phenotypes of Notch signaling were reverted by transposon excision experiments of <em>mf157</em>. In conclusion, we identify three fresh alleles of <em>Ser</em>. Our works supply additional genetic resources for further study of functions of <em>Ser</em> and Notch signaling regulation.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"177 ","pages":"Article 203908"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667290124000093/pdfft?md5=b1f8832b2416076c695a254db66864b7&pid=1-s2.0-S2667290124000093-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial stem and progenitor cells of the upper airway","authors":"Maral J. Rouhani ,&nbsp;Sam M. Janes ,&nbsp;Carla F. Kim","doi":"10.1016/j.cdev.2024.203905","DOIUrl":"10.1016/j.cdev.2024.203905","url":null,"abstract":"<div><p>The upper airway acts as a conduit for the passage of air to the respiratory system and is implicated in several chronic diseases. Whilst the cell biology of the distal respiratory system has been described in great detail, less is known about the proximal upper airway. In this review, we describe the relevant anatomy of the upper airway and discuss the literature detailing the identification and roles of the progenitor cells of these regions.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"177 ","pages":"Article 203905"},"PeriodicalIF":3.9,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667290124000068/pdfft?md5=80c073a8067ce7d4b678ed2e5eb0918d&pid=1-s2.0-S2667290124000068-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139736293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic approaches of cell therapy based on stem cells and terminally differentiated cells: Potential and effectiveness","authors":"Ali Pirsadeghi ,&nbsp;Negar Namakkoobi ,&nbsp;Mahtab Sharifzadeh Behzadi ,&nbsp;Hanieh Pourzinolabedin ,&nbsp;Fatemeh Askari ,&nbsp;Erfan Shahabinejad ,&nbsp;Somayeh Ghorbani ,&nbsp;Fatemeh Asadi ,&nbsp;Ali Hosseini-Chegeni ,&nbsp;Aliakbar Yousefi-Ahmadipour ,&nbsp;Mohammad Hossein Kamrani","doi":"10.1016/j.cdev.2024.203904","DOIUrl":"10.1016/j.cdev.2024.203904","url":null,"abstract":"<div><p>Cell-based therapy, as a promising regenerative medicine approach, has been a promising and effective strategy to treat or even cure various kinds of diseases and conditions. Generally, two types of cells are used in cell therapy, the first is the stem cell, and the other is a fully differentiated cell. Initially, all cells in the body are derived from stem cells. Based on the capacity, potency and differentiation potential of stem cells, there are four types: totipotent (produces all somatic cells plus perinatal tissues), pluripotent (produces all somatic cells), multipotent (produces many types of cells), and unipotent (produces a particular type of cells). All non-totipotent stem cells can be used for cell therapy, depending on their potency and/or disease state/conditions. Adult fully differentiated cell is another cell type for cell therapy that is isolated from adult tissues or obtained following the differentiation of stem cells. The cells can then be transplanted back into the patient to replace damaged or malfunctioning cells, promote tissue repair, or enhance the targeted organ's overall function. With increasing science and knowledge in biology and medicine, different types of techniques have been developed to obtain efficient cells to use for therapeutic approaches. In this study, the potential and opportunity of use of all cell types, both stem cells and fully differentiated cells, are reviewed.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"177 ","pages":"Article 203904"},"PeriodicalIF":3.9,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667290124000056/pdfft?md5=c75d3ebfc23968965d908efd5129ded8&pid=1-s2.0-S2667290124000056-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139693148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphoelastic models discriminate between different mechanisms of left-right asymmetric stomach morphogenesis","authors":"Ariel N. Nikas,&nbsp;Evan J. Curcio,&nbsp;Nanette Nascone-Yoder,&nbsp;Sharon R. Lubkin","doi":"10.1016/j.cdev.2024.203902","DOIUrl":"10.1016/j.cdev.2024.203902","url":null,"abstract":"<div><p>The mechanisms by which the vertebrate stomach undergoes its evolutionarily conserved leftward bending remain incompletely understood. Although the left and right sides of the organ are known to possess different gene expression patterns and undergo distinct morphogenetic events, the physical mechanisms by which these differences generate morphological asymmetry remain unclear. Here, we develop a continuum model of asymmetric stomach morphogenesis. Using a morphoelastic framework, we investigate the morphogenetic implications of a variety of hypothetical, tissue-level growth differences between the left and right sides of a simplified tubular organ. Simulations reveal that, of the various differential growth mechanisms tested, only one category is consistent with the leftward stomach curvature observed in wild-type embryos: equal left and right volumetric growth rates, coupled with transversely isotropic tissue thinning on the left side. Simulating this mechanism in a defined region of the model over a longer period of growth leads to mature stomach-like curvatures.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"177 ","pages":"Article 203902"},"PeriodicalIF":3.9,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667290124000032/pdfft?md5=4bae8734221fb0c91f388badc2a15bbe&pid=1-s2.0-S2667290124000032-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive nitrogen species-mediated cell proliferation during tail regeneration and retinoic acid as a putative modulator of tissue regeneration in the geckos","authors":"A. Sarada Achary,&nbsp;Cuckoo Mahapatra","doi":"10.1016/j.cdev.2024.203901","DOIUrl":"10.1016/j.cdev.2024.203901","url":null,"abstract":"<div><p>Reactive nitrogen species (RNS), a mediator of nitrosative stress, plays a vital role during wound healing but its role during tissue regeneration is poorly understood. In the present study, the role of RNS was investigated post-tail autotomy and limb amputation in a gecko species, <em>Hemidactylus murrayi</em> Gleadow, 1887. Tail autotomy led to an increased expression of <em>iNOS</em> and nitrosative stress leading to protein S-nitrosylation that probably restricted the acute inflammatory response caused by wounding. Increased nitrosative stress was also associated with proliferation of the wound epithelium and the tail blastema. Nitric oxide synthase inhibitor (L-NAME) caused retarded growth and structural abnormalities in the regenerating tail while peroxynitrite inhibitor (FeTmPyp) arrested tail regeneration. Spermine NONOate and retinoic acid, used as NO donors generated small outgrowths post-amputation of limbs with an increased number of proliferating cells and s-nitrosylation indicating the role of nitric oxide signalling in cell proliferation during regeneration. Additionally, retinoic acid treatment caused regeneration of nerve, muscle and adipose tissue in the regenerated limb structure 105 days post-amputation suggesting it to be a putative modulator of tissue regeneration in the non-regenerating limbs.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"177 ","pages":"Article 203901"},"PeriodicalIF":3.9,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667290124000020/pdfft?md5=6ea72102ee2a11aee88a8441e814a2dc&pid=1-s2.0-S2667290124000020-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Live imaging of Fibronectin 1a-mNeonGreen and Fibronectin 1b-mCherry knock-in alleles during early zebrafish development","authors":"Dörthe Jülich,&nbsp;Scott A. Holley","doi":"10.1016/j.cdev.2024.203900","DOIUrl":"10.1016/j.cdev.2024.203900","url":null,"abstract":"<div><p>Within the developing embryo, cells assemble and remodel their surrounding extracellular matrix during morphogenesis. Fibronectin is an extracellular matrix glycoprotein and is a ligand for several members of the Integrin adhesion receptor family. Here, we compare the expression pattern and loss of function phenotypes of the two zebrafish <em>fibronectin</em> paralogs <em>fn1a</em> and <em>fn1b</em>. We engineered two fluorescently tagged knock-in alleles to facilitate live <em>in vivo</em> imaging of the Fibronectin matrix. Genetic complementation experiments indicate that the knock-in alleles are fully functional. Fn1a-mNeonGreen and Fn1b-mCherry are co-localized in ECM fibers on the surface of the paraxial mesoderm and myotendinous junction. In 5-days old zebrafish larvae, Fn1a-mNeonGreen predominantly localizes to the branchial arches, heart ventricle, olfactory placode and within the otic capsule while Fn1b-mCherry is deposited at the pericardium, proximal convoluted tubule, posterior hindgut and at the ventral mesoderm/cardinal vein. We examined Fn1a-mNeonGreen and Fn1b-mCherry in maternal zygotic <em>integrin α5</em> mutants and <em>integrin β1a; β1b</em> double mutants and find distinct requirements for these Integrins in assembling the two Fibronectins into ECM fibers in different tissues. Rescue experiments via mRNA injection indicate that the two <em>fibronectins</em> are not fully inter-changeable. Lastly, we examined cross-regulation between the two Fibronectins and find <em>fn1a</em> is necessary for normal Fn1b fibrillogenesis in the presomitic mesoderm, but <em>fn1b</em> is dispensable for the normal pattern of Fn1a deposition.</p></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"177 ","pages":"Article 203900"},"PeriodicalIF":3.9,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667290124000019/pdfft?md5=5f5514dfc9a44ca873d6e53d7bdc9812&pid=1-s2.0-S2667290124000019-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}