Roczniki Panstwowego Zakladu Higieny最新文献

{"title":"Negative impact of smoking on lung function: comparing FEV1/FVC values in smokers and non-smokers.","authors":"Kittithat Sudchoo,&nbsp;Sukrit Sangkhano,&nbsp;Aruna Prasopthum,&nbsp;Phisit Pouyfung","doi":"10.32394/rpzh.2023.0265","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0265","url":null,"abstract":"<p><strong>Background: </strong>Cigarette smoking has been reported as the significant adverse effects on lung function, which can be evaluated by measuring forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and the ratio of FEV1 to FVC (FEV1/FVC) values.</p><p><strong>Objective: </strong>This study investigated the prevalence of tobacco use and nicotine dependence among college students and their stress levels to inform targeted interventions for smoking prevention and cessation.</p><p><strong>Material and methods: </strong>A total of 429 participants were interviewed face-to-face, of which 71.6% were female and 28.4% were male. Pulmonary function tests were exclusively administered to the 9.8% of participants who reported using tobacco.</p><p><strong>Results: </strong>Our findings revealed a low prevalence of nicotine dependence among college students, with only 6.8% exhibiting moderate or high levels of dependence. Students who reported tobacco use were found to have moderate stress levels,suggesting a potential association between smoking and stress. Chi-square tests revealed that gender, school affiliation,and nicotine dependence were significantly associated with smoking behavior. Long-term smoking (>5 years) was found to be associated with negative health outcomes, such as higher BMI, and increased smoking per day. The analysis of lung function parameters showed that smoking frequency and duration were negatively associated with lung function, while nicotine dependence increased with smoking frequency and duration.</p><p><strong>Conclusion: </strong>Our study suggests that targeted prevention and cessation programs should address these factors to reduce smoking rates among college students.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10226193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Energy expenditure during training and official league match in professional female soccer players - a pilot study.","authors":"Hubert Dobrowolski,&nbsp;Dariusz Włodarek","doi":"10.32394/rpzh.2023.0253","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0253","url":null,"abstract":"<p><strong>Background: </strong>The most important component of a well-balanced diet is the proper energetic value. However, adequate estimation of the body's energy needs is difficult for professional athletes, including soccer players. There is little research showing energy expenditure during training and lack of studies on the energy expenditure of professional female soccer players during a match.</p><p><strong>Objective: </strong>The aim of our study was to estimate energy expenditure during training and official league match in female soccer players and comparing it.</p><p><strong>Material and methods: </strong>Seven Polish professionally practicing soccer females (23.4±6,6 years old; 63.5±7.8 kg; 168.5±5.8 cm; 46±4.4 kg fat-free mass) participated in the study. The participants had their height and body mass measured. Energy expenditure during activities was measured by means of a SenseWear Pro3 Armband device. Body composition was assessed with Akern BIA 101 Anniversary Sport Edition device.</p><p><strong>Results: </strong>Statistically higher energy expenditure was achieved in the study group during the match hour (452±55 kcal/hour) compared to the training hour (353±28 kcal/ hour) as well as in the case of energy expenditure per hour of activity per kg of fat-free mass (match: 9.94±1.75 kcal/kg fat-free mass/hour; training: 7.71±0.8 kcal/kg fat-free mass/hour). During one hour of training, more time was spent on sedentary, light, and moderate activities, but the difference was statistically significant only for light activities. More time during the match hour than during the training hour was spent on vigorous and very vigorous activities.</p><p><strong>Conclusions: </strong>In conclusion, the energy expenditure of the players during the match was greater than in the case of the planned intensive training, which was caused by the timeshare of more intense physical activities and going a longer distance during match.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intranasal (IN) COVID-19 vaccines - a breakthrough.","authors":"Ramandeep Singh Gambhir,&nbsp;Khushdeep Kaur,&nbsp;Ridhi Matariya,&nbsp;Balwinder Singh,&nbsp;Ridhima Sood,&nbsp;Jyotsana Singh","doi":"10.32394/rpzh.2023.0251","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0251","url":null,"abstract":"<p><p>Emerging variants of COVID-19 have threatened the effectiveness of intramuscular (IM) vaccines since that are made to target only the spike protein. Development of Intranasal (IN) vaccination has been proven to provide both the mucosal and systemic immune responses for broader and long lasting protection. Many IN vaccine candidates (virus-vectored vaccines, recombinant subunit vaccines and live attenuated vaccines) are in different phases of clinical trials and in near future many companies would be releasing their vaccines into the drug market. Potential advantages of IN vaccination over IM vaccination makes them ideal to be administered in children and developing populations of the world. This paper focuses on the very recent developments in intranasal vaccination with a spotlight on their safety and efficacy concerns. IN vaccination can prove to be game-changer in handling COVID-19 and potential viral contagious diseases in future.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9616364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional and antioxidant profile of the Physalis fruit grown in three Andean regions of Peru.","authors":"Antonio José Obregón-La Rosa,&nbsp;Eliana Contreras-López,&nbsp;Eduardo Flores Juárez,&nbsp;Úrsula Gonzales Barrón,&nbsp;Ana María Muñoz,&nbsp;Fernando Ramos-Escudero","doi":"10.32394/rpzh.2023.0247","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0247","url":null,"abstract":"<p><strong>Background: </strong>Physalis peruviana L. fruit contains nutritional and bioactive compounds of immense importance to public health and represents a potential ingredient for the development of functional foods and beverages.</p><p><strong>Objective: </strong>This study aimed to determine the chemical and nutritional composition as well as the antioxidant capacity of the P. peruviana L. fruit grown in Peru in three areas of the Central Andean region.</p><p><strong>Material and methods: </strong>Proximal and physicochemical analyses and estimation of mineral content, vitamin C, total carotenoids, total polyphenols, and antioxidant capacity (2, 2-diphenyl-1-picrylhydrazyl [DPPH] and 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) [ABTS] assays) were performed using standardized methods.</p><p><strong>Results: </strong>The fruits were collected from three regions of the Peruvian Andes (Ancash, Cajamarca, and Cusco). The results showed that the content of potassium (306.54-327.60 mg/100 g) and iron (12.93-14.47 mg/kg) was prominent. The Physalis fruit had high levels of vitamin C (47.20-52.20 mg/100 g), total polyphenols (68.17-83.40 mg equivalents of gallic acid/100 g), and carotenoids (1.12-1.73 mg β-carotene/100 g). Higher values for antioxidant capacity were obtained with the ABTS method (896-1003.33 μmol Trolox/100 g) than with the DPPH method (290-309 μmol Trolox/100 g).</p><p><strong>Conclusions: </strong>This study confirms that the P. peruviana fruit has properties that could provide important health benefits and that it could be used for the development of functional foods and food supplement.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9616371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross sectional study of vitamin B12 supplementation in Slovak and Czech vegans.","authors":"Radek Latal,&nbsp;Marta Habanova,&nbsp;Eliska Selinger,&nbsp;Maros Bihari,&nbsp;Jadwiga Hamulka","doi":"10.32394/rpzh.2023.0259","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0259","url":null,"abstract":"<p><strong>Background: </strong>The number of vegans in the world is growing and in Slovakia and the Czech Republic they make up 1% of the population. Vegan diet excludes all foods of animal origin and vegans who do not use vitamin B12 supplements are at risk of the vitamin B12 deficiency.</p><p><strong>Objective: </strong>The aim of this study was to determine what proportion of Czech and Slovak vegans use vitamin B12 supplements regularly, irregularly or not at all and what is their supplemental cobalamin intake.</p><p><strong>Materials and methods: </strong>The research involved 1337 self-identified vegans from Slovakia and the Czech Republic who were interviewed using the CAWI (Computer-Assisted Web Interview) method. Participants were recruited by posts in veganism-themed social media groups.</p><p><strong>Results: </strong>Out of 1337 vegans 55.5% supplemented cobalamin regularly, 32.54% irregularly and 11.97% were not supplementing. Rate of not supplementing individuals was 5.04% higher in Slovaks than in Czechs. Short-term vegans had a significantly higher rate of not supplementing individuals (17.99%) compared to medium-term (8.37%) and long-term vegans (7.50%). Mean weekly cobalamin intake from supplements was 2938.34±2566.60 μg in regularly supplementing vegans compared to 1630.31±1949.27 μg in irregularly supplementing vegans, particularly due to the lower weekly supplementation frequency among irregularly (2.93) compared to regularly supplementing vegans (5.27).</p><p><strong>Conclusions: </strong>The rate of supplementation in Slovak and particularly Czech vegans was higher than in other countries. The number of not supplementing individuals was significantly higher among short-term vegans, indicating that there is still a need for education on the importance of adequate and regular cobalamin supplementation, especially in new vegans. Our results support the hypothesis that the reason for higher rate of cobalamin deficiency in irregularly compared to regularly supplementing vegans is the lower cobalamin intake caused by lower supplementation frequency.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9627333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of genistein supplemented before or after irradiation on DNA injury in human lymphocytes in vitro.","authors":"Małgorzata M Dobrzyńska, Aneta Gajowik","doi":"10.32394/rpzh.2023.0280","DOIUrl":"10.32394/rpzh.2023.0280","url":null,"abstract":"<p><strong>Background: </strong>Ionizing radiation (IR) carry adequate energy to ionize or remove electrons from an atom. Particles interact with water to produce reactive oxygen species (ROS). Genistein (GEN) is a naturally occurring phytoestrogen and the basic isoflavonoid in soybeans and soybean-enriched products and is believed to have the strongest antioxidant activity.</p><p><strong>Objective: </strong>The study aimed at the investigation if application of GEN at different time prior or past irradiation may ameliorate or reduce injury of DNA in human lymphocytes.</p><p><strong>Material and methods: </strong>The isolated lymphocytes were exposed to X-irradiation (0.5; 1 Gy). GEN (1 µM/ml; 10 µM/ ml) was appended to attempts at various times prior or past irradiation (1 h prior, immediately prior, immediately past, 1 h past). We joined each X-rays dose with each GEN dose. After 1h of incubation DNA damages were examined using Comet assay.</p><p><strong>Results: </strong>Combination of 1 µM/ml of GEN given 1 h before irradiation with low or high dose markedly decreased induced by irradiation DNA injury. Higher dose of GEN applied immediately before or after irradiation markedly extended the frequency of DNA injury generated by irradiation. The result of application 1 µM/ml GEN 1 h after irradiation was not significantly different compared to control. The effect of 1 Gy + 10 µM/ml GEN was not significantly lower compared to each agent alone.</p><p><strong>Conclusions: </strong>Only a very low concentration of GEN applied before irradiation, may be considered as a potential radiomitigator/radioprotector. High doses of GEN work as a radiosentitizer and may potent the effects of radiotherapy.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors, erythrocyte acetylcholinesterase inhibition, and self-reported symptoms of pesticide intoxication among farmers in Thailand: A cross-sectional study.","authors":"Ekarat Sombatsawat,&nbsp;Wattasit Siriwong,&nbsp;Sitthichok Puangthongthub","doi":"10.32394/rpzh.2023.0249","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0249","url":null,"abstract":"<p><strong>Background: </strong>Organophosphate and carbamate pesticides are widely used, and their adverse health effects remain a serious problem.</p><p><strong>Objectives: </strong>This investigation aimed to describe risk factors, erythrocyte acetylcholinesterase (AChE) inhibition, and self-reported symptoms and to derive an association between influence factors and erythrocyte AChE inhibition among farmers in Thailand.</p><p><strong>Methods: </strong>A cross-sectional study was conducted on 71 farmers from August to October 2022. General characteristics and pesticide exposure factors were elicited via a questionnaire-based interview. The erythrocyte AChE inhibition was assessed using the EQM Test-mate Cholinesterase (Model 400) instrument. Data were presented descriptively and analyzed statistically using Chi-square and binary logistic regression.</p><p><strong>Results: </strong>Most farmers were over 50 years old and had an abnormal body mass index (BMI) without alcohol consumption and smoking. Aprons (18.31%) and protective eyewear (12.68%) as personal protective equipment (PPE) were found to be used less often. The level of hemoglobin-adjusted erythrocyte AChE (Q) was considered normal when it was 59.15% and abnormal when it was 40.85%. Self-reported symptoms were confirmed to be associated with lower erythrocyte AChE levels. The Chi-square analysis showed that shortness of breath, irritation, headache, dizziness, sleep fragmentation, and memory problems were significantly associated with erythrocyte AChE (p < 0.05). The bivariate analysis revealed that farmers who consumed alcohol while using pesticides (mixing, loading, and spraying) (OR=35.821, 95% CI=4.591-279.490), who did not wear a mask while using pesticides (OR=11.898, 95% CI=1.061-133.440), and who did not wear boots while using pesticides (OR=0.166, 95% CI=0.031-0.890) had an increased likelihood of having a severe inhibition of erythrocyte AChE.</p><p><strong>Conclusions: </strong>These findings suggest that the promotion of risk prevention practices must be imposed on appropriate pesticide handling and PPE use among farmers.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9248525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Body mass index, functional fitness and nutritional behaviours of senior women from the Kraków population.","authors":"Maria Gacek,&nbsp;Agnieszka Wojtowicz,&nbsp;Grażyna Kosiba,&nbsp;Magdalena Majer,&nbsp;Joanna Gradek","doi":"10.32394/rpzh.2023.0240","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0240","url":null,"abstract":"<p><strong>Background: </strong>Body Mass Index (BMI) is dependent on, among others, diet and level of physical activity. Seniors are more prone to nutritional disorders than other population groups.</p><p><strong>Objective: </strong>The aim of the study was to analyse the relationship between BMI and nutritional behaviours as well as the functional fitness level of senior women.</p><p><strong>Materials and methods: </strong>The research was carried out among a group of 120 women aged 60-84, using the TANITA SC-330ST body composition analyser, the HOLTAIN anthropometer, the Fullerton Functional Fitness Test and the authordesigned nutritional behaviour questionnaire for seniors. Statistical analyses were conducted using the IBM SPSS 21 statistical package, applying the Kruskal-Wallis ANOVA tests with comparisons of z tests at the significance level p<0.05.</p><p><strong>Results: </strong>In terms of the relationship between BMI and functional fitness indices, it was shown that women with normal weight obtained higher results for the lower body (p=0.043) and upper body agility tests than obese women (p<0.001). Females with normal BMI also obtained higher results in the endurance test than the overweight women (p=0.038). In terms of the correlation between BMI and nutritional behaviours, it was demonstrated that women with a healthy body mass more often ate varied, low-volume meals than those overweight (p=0.026). Women with correct weight ate fish, eggs and lean meat more often than obese women (p=0.036). Obese women consumed 3-5 portions of fruit and vegetables less frequently during the day than women with normal body mass (p=0.029) and those overweight (p=0.015). Obese women were less likely to eat sea fish at least 1-2 times a week than overweight females (p=0.040) and those with normal BMI (p<0.001). At the same time, women with a normal BMI indicated a higher degree of performed daily physical activity than the overweight women (p=0.028) and those with obesity (p=0.030).</p><p><strong>Conclusions: </strong>Women with normal BMI presented more rational nutrition habits and higher functional fitness than overweight and obese senior women.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9616366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lutein and zeaxanthin - radio- and chemoprotective properties. Mechanism and possible use.","authors":"Jakub Fiedorowicz,&nbsp;Małgorzata M Dobrzynska","doi":"10.32394/rpzh.2023.0266","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0266","url":null,"abstract":"<p><p>Lutein and zeaxanthin are naturally occurring xanthophylls, mainly present in green, leafy vegetables and egg's yolk. Their presence is connected with blue spectrum light absorbance, including UV. This property, and fact, that these xanthophylls are accumulated by human eye's macula, leads to eye's protective functions of them including protection from age-related macular degeneration (AMD). Also, antioxidative features of lutein and zeaxanthin are boosting overall health of human body. Numerous studies proves anti-inflammatory and protective attributes of these compounds, based on many, different mechanisms. One of them is regulating redox potential in cells, and impact on expression of linked genes. In preventing of eye diseases, an important gene that is regulated by lutein and zeaxanthin is the Nrf2 gene, whose increased activity leads to optimizing the cellular response to reactive oxygen species (ROS) and preventing related diseases. Other research confirms antiproliferative properties of mentioned compounds in case of certain human cancer cell lines. There are e.g.: HepG2 (hepatitis cancer), MCF-7 (breast cancer), which treated in vitro with lutein solution showed reduction of cell growth. Lutein alone, during in vivo studies conducted on mice, exhibited also radioprotective properties, positively affecting the vitality of animals. Lutein provides also increasing of tolerance to UV radiation, reducing inflammatory processes in the skin and preventing oncogenesis. Low intake of lutein and zeaxanthin, associated with \"western diet\", rich in simple carbohydrates and processed food, common in developed countries, including Poland, is linked with diabetes and obesity incidence. Assuming, lutein and zeaxanthin significantly affect the well-being of the human body, and their appropriate amount in diet can help reduce risk of many diseases. For supplementation, the optimized dosage of these xanthophylls includes doses of 10 mg for lutein and 2 mg for zeaxanthin, and it is recommended to consume along with fats or meals rich in fats.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10208461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological and physicochemical quality of raw milk of Beni Mellal-Khenifra.","authors":"Nysrine Mannani,&nbsp;Najat Ariri,&nbsp;Abdelali Bitar","doi":"10.32394/rpzh.2023.0267","DOIUrl":"https://doi.org/10.32394/rpzh.2023.0267","url":null,"abstract":"<p><strong>Background: </strong>Milk is a nutritious food for humans, but it is also an ideal growth medium for pathogenic bacteria such as Salmonella, Listeria monocytogenes or Campylobacter spp, which can cause food poisoning.</p><p><strong>Objective: </strong>For this reason, we evaluated the microbiological, physicochemical and hygienic quality of raw milk samples in 9 milk collection centres and cooperatives in the Beni-Mellal-Khenifr region before and after the implementation of the quality management system, hazard analysis and critical control points (HACCP).</p><p><strong>Materials and method: </strong>The microbiological and physico-chemical quality of 184 raw milk samples in 9 milk collection centres and cooperatives in the Beni-Mellal-Khenifra region was evaluated before and after the implementation of the quality management, hazard analysis and critical control points (HACCP) system.</p><p><strong>Results: </strong>The physico-chemical results obtained after the implementation of HACCP revealed that 78% of the samples analysed were below the maximum limits authorised at international level. A pH between 6.6 ±0.11 and 6.8±0.02, an acidity of 15°D, the milk is stable according to the Ramsdell test which is negative at 0.9. The temperature remains within the acceptance range with an average of 6.62 ±0.45°C to 7.7 ±0.36°C and the fat content is between 33 and 45g/ml. We also noted an improvement in the microbiological quality of milk in 81% of collection centres and cooperatives. The level of contamination by total coliforms is from 2.5x104 to 11.5x104 CFU/ml lower than the Moroccan standard (NM 08.0. 100) of 1.5x104 CFU/ml to 20.3x104 CFU/ml, the total aerobic mesophilic flora and the psychrotrophs have an average of 2.5x105 germs/ml to 82x105 germs/ml lower than before improvement (12.5x105 germs/ml to 192x105 germs/ml).</p><p><strong>Conclusion: </strong>We can conclude that the work done leads to an improvement and satisfaction of the quality of raw milk compared to the standard after the implementation of HACCP.</p>","PeriodicalId":35951,"journal":{"name":"Roczniki Panstwowego Zakladu Higieny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10568440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}