N. Muralimohan, Vivek kumar Singh, Maniraj Rathinam, K. Kasturi, R. Sreevathsa
{"title":"Protein Engineering Of Bt Genes cry1Ab And cry1Ba For The Development Of Chimeric Genes cryAbabba, cryBabaab And cryAbbaab Via Domain Swapping","authors":"N. Muralimohan, Vivek kumar Singh, Maniraj Rathinam, K. Kasturi, R. Sreevathsa","doi":"10.53555/jaz.v45i3.4137","DOIUrl":"https://doi.org/10.53555/jaz.v45i3.4137","url":null,"abstract":"Bacillus thuringiensis is renowned for its production of insecticidal cry proteins, widely utilized in crop protection to combat insects. However, the risk of insect resistance emerges due to the relatively loose binding of toxins to target sites on larvae's midgut brush boundary membranes. This resistance primarily stems from modifications in binding sites within midgut cells. To address potential threats, the discovery of new Cry proteins is imperative as insects continually evolve resistance against existing ones. Combining Cry toxins with diverse binding sites in larval midguts is proposed as an effective strategy to delay the onset of resistance. In this study, three chimeric B. thuringiensis proteins—CryAbAbBa, CryBaBaAb, and CryAbBaAb—were engineered via domain swapping, utilizing crystal proteins CrylAb and CrylB. Structural validation was conducted, confirming their integrity through Ramachandran Plots. The chimeric proteins can be used as additional resources in crop improvement programmes.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"52 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140082593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physicochemical Investigation to Probe the Recovery and Controlling Management of Gout Pain in Human Body through Accumulation of Vitamin B6 Molecule","authors":"Dr. Habibur Rahaman","doi":"10.53555/jaz.v45i2.3757","DOIUrl":"https://doi.org/10.53555/jaz.v45i2.3757","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"183 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139847240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Susmita Saha, Sanya Khurana, P. Aneja, Paras Kumar, Nisha Pahal, Vikash Bhoria
{"title":"Morphometric description of greater & lesser tubercles at proximal humerus: An Anatomical study","authors":"Susmita Saha, Sanya Khurana, P. Aneja, Paras Kumar, Nisha Pahal, Vikash Bhoria","doi":"10.53555/jaz.v45i2.3824","DOIUrl":"https://doi.org/10.53555/jaz.v45i2.3824","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":" 1047","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139787117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Garima Bajpai, Vivek Dabral, Prof. (Dr) Reena Singh, Prof. (Dr) H.C. Purohit
{"title":"A Systematic Literature Review On The Impact Of Ethical Leadership On Employee Job Performance","authors":"Garima Bajpai, Vivek Dabral, Prof. (Dr) Reena Singh, Prof. (Dr) H.C. Purohit","doi":"10.53555/jaz.v45i2.3836","DOIUrl":"https://doi.org/10.53555/jaz.v45i2.3836","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"35 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139846812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Susmita Saha, Sanya Khurana, P. Aneja, Paras Kumar, Nisha Pahal, Vikash Bhoria
{"title":"Morphometric description of greater & lesser tubercles at proximal humerus: An Anatomical study","authors":"Susmita Saha, Sanya Khurana, P. Aneja, Paras Kumar, Nisha Pahal, Vikash Bhoria","doi":"10.53555/jaz.v45i2.3824","DOIUrl":"https://doi.org/10.53555/jaz.v45i2.3824","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"12 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139846929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monu Yadav, M. Parle, Deepak Lamba, Sumit Kumar, Mini Dahiya, Kajal Joshi
{"title":"Neuroprotective effect of Dark Chocolate on ketamine-induced animal model of psychosis through the reversal of behavioral, biochemical and histopathological changes","authors":"Monu Yadav, M. Parle, Deepak Lamba, Sumit Kumar, Mini Dahiya, Kajal Joshi","doi":"10.53555/jaz.v45i2.3447","DOIUrl":"https://doi.org/10.53555/jaz.v45i2.3447","url":null,"abstract":"The present study was designed to evaluate the antipsychotic-like activity of dark chocolate against Ketamine-induced psychosis in animals and to explore the possible underlying mechanisms for this activity. Dark chocolate (200, 400 mg/kg, along with diet), haloperidol (1mg/kg, i.p.), and olanzapine (5mg/kg, i.p) were administered daily to different groups of mice and rats; after 30 min Ketamine was injected for 21 successive days. The antipsychotic- like activity was assessed using an actophotometer, stereotypic behaviors, forced swim test (FST), and passive avoidance test. Dark Chocolate (DC) and olanzapine remarkably decreased the immobility period of Ketamine-treated mice in FST, indicating the beneficial effect of DC against depressive or negative symptoms of psychosis. There was no significant effect on the locomotor activity of the mice with the administration of DC, while it showed a reducing impact against stereotypic behaviors. Furthermore, DC significantly reduced the time taken by the rats to climb the pole, showing the effect of DC on cognition. In addition, acetylcholinesterase (AChE) activity, malondialdehyde (MDA), and tissue necrosis factor (TNF-α) levels were markedly decreased and increased GABA and enhanced reduced glutathione (GSH) levels with the treatment of DC. Furthermore, DC was also effective in reducing the ketamine induced hyperchromatic nuclei, perinuclear vacuolization, and dilated vascular channels in the cortex part of the brain. Behavioral, biochemical, and histopathological studies suggest the protective effect of DC against Ketamine induced psychosis.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"13 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139854295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monu Yadav, M. Parle, Deepak Lamba, Sumit Kumar, Mini Dahiya, Kajal Joshi
{"title":"Neuroprotective effect of Dark Chocolate on ketamine-induced animal model of psychosis through the reversal of behavioral, biochemical and histopathological changes","authors":"Monu Yadav, M. Parle, Deepak Lamba, Sumit Kumar, Mini Dahiya, Kajal Joshi","doi":"10.53555/jaz.v45i2.3447","DOIUrl":"https://doi.org/10.53555/jaz.v45i2.3447","url":null,"abstract":"The present study was designed to evaluate the antipsychotic-like activity of dark chocolate against Ketamine-induced psychosis in animals and to explore the possible underlying mechanisms for this activity. Dark chocolate (200, 400 mg/kg, along with diet), haloperidol (1mg/kg, i.p.), and olanzapine (5mg/kg, i.p) were administered daily to different groups of mice and rats; after 30 min Ketamine was injected for 21 successive days. The antipsychotic- like activity was assessed using an actophotometer, stereotypic behaviors, forced swim test (FST), and passive avoidance test. Dark Chocolate (DC) and olanzapine remarkably decreased the immobility period of Ketamine-treated mice in FST, indicating the beneficial effect of DC against depressive or negative symptoms of psychosis. There was no significant effect on the locomotor activity of the mice with the administration of DC, while it showed a reducing impact against stereotypic behaviors. Furthermore, DC significantly reduced the time taken by the rats to climb the pole, showing the effect of DC on cognition. In addition, acetylcholinesterase (AChE) activity, malondialdehyde (MDA), and tissue necrosis factor (TNF-α) levels were markedly decreased and increased GABA and enhanced reduced glutathione (GSH) levels with the treatment of DC. Furthermore, DC was also effective in reducing the ketamine induced hyperchromatic nuclei, perinuclear vacuolization, and dilated vascular channels in the cortex part of the brain. Behavioral, biochemical, and histopathological studies suggest the protective effect of DC against Ketamine induced psychosis.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"36 S158","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139794545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"COMPARATIVE ANALYSIS OF SENSILLA ON ANTENNAE AND MOUTHPARTS OF LARVAE IN TWO SPECIES OF DITRYSIAN MOTHS (LEPIDOPTERA)","authors":"A. S. Kaleka, Harsimrat Kaur Dulai","doi":"10.53555/jaz.v45i1.2915","DOIUrl":"https://doi.org/10.53555/jaz.v45i1.2915","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139864750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"COMPARATIVE ANALYSIS OF SENSILLA ON ANTENNAE AND MOUTHPARTS OF LARVAE IN TWO SPECIES OF DITRYSIAN MOTHS (LEPIDOPTERA)","authors":"A. S. Kaleka, Harsimrat Kaur Dulai","doi":"10.53555/jaz.v45i1.2915","DOIUrl":"https://doi.org/10.53555/jaz.v45i1.2915","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"6 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139804963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Revolutionizing Drug Delivery: The Role of Nanofibers - A Review","authors":"Niyati Shah, Ritu Sapra, Mamta Kumari, Piyushkumar Sadhu, Chitrali R. Talele","doi":"10.53555/jaz.v45i1.3347","DOIUrl":"https://doi.org/10.53555/jaz.v45i1.3347","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":"19 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139531899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}