中国实验血液学杂志最新文献

{"title":"[Effects of <i>PIM1</i> Gene on Proliferation, Apoptosis and JAK2/STAT3 Signaling Pathway of Acute Myeloid Leukemia U937 Cells].","authors":"Xin Gao, Li-Jing Chu, Zong-Hai Yan","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.003","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.03.003","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of the serine/threonine kinase family member 1 (<i>PIM1</i>) gene on the proliferation and apoptosis of acute myeloid leukemia (AML) U937 cells, and the regulation effect on Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway.</p><p><strong>Methods: </strong>Bone marrow mononuclear cells from newly diagnosed adult AML patients and patients with iron deficiency anemia were collected and <i>PIM1</i> mRNA expression was detected by RT-qPCR. AML cell line U937 cells were divided into U937 group (U937 cells were cultured normally), Si-PIM1 group (U937 cells were transfected with low expression adenovirus vector containing <i>PIM1</i> mRNA), Si-NC group (U937 cells were transfected with low expression adenovirus vector without <i>PIM1</i> mRNA), coumermycin A1 (CoA1) group (JAK2 activator CoA1 was added to U937 cells at a concentration of 20 μmol/L), and Si-PIM1+CoA1 group (U937 cells were transfected with adenoviral vector containing low expression of <i>PIM1</i> mRNA and added with CoA1 at a concentration of 20 μmol/L). After culture for 24 h, the expressions of <i>PIM1</i> mRNA and protein, JAK2/STAT3 pathway, cell cycle and apoptosis-related proteins in U937 cells were detected by RT-qPCR and Western blot, the cell proliferation activity was detected by MTT assay, and flow cytometry was used to detect cell cycle changes and apoptosis rate.</p><p><strong>Results: </strong>The <i>PIM1</i> mRNA expression level in bone marrow mononuclear cells in AML patients was higher than that in patients with iron deficiency anemia (<i>P</i> < 0.05). Compared with U937 group, <i>PIM1</i> mRNA and protein, phosphorylated JAK2 (p-JAK2)/JAK2, phosphorylated STAT3 (p-STAT3)/STAT3, Cyclin D1, cyclin-dependent kinase 2 (CDK2) protein, cell proliferation activity, S phase and G ₂/M phase proportions were decreased in Si-PIM1 group (all <i>P</i> < 0.05), while p27, Caspase-3 protein, G₀/G₁ phase proportion and apoptosis rate were increased (all <i>P</i> < 0.05). However, the changes of above indicators in CoA1 group were just opposite to those in Si-PIM1 group, indicating that CoA1 could reverse the effect of Si-PIM1 on U937 cells. There were no significant differences in above indexes of U937 cells between U937 group, Si-PIM1+CoA1 group and Si-NC group (<i>P</i> >0.05).</p><p><strong>Conclusion: </strong>Knockdown of <i>PIM1</i> gene expression can inhibit U937 cell proliferation and promote apoptosis, in order to alleviate ALM process, which may be related to the inhibition of JAK2/STAT3 pathway activation.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research Progress on Role of Long Non-Coding RNA in Occurrence and Development of Acute Myeloid Leukemia--Review].","authors":"Xiao-Shi Lu, Xin-Qi Wang, Ying-Nan DU, Ji-Hong Zhang","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.051","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.051","url":null,"abstract":"<p><p>In recent years, the importance of long non-coding RNA (lncRNA) in acute myeloid leukemia (AML) has attracted wide attention. Among them, lncRNAs that play a role in promoting cancer mainly include <i>HOTAIR, UCA1, H19, ITGB2-AS1</i> and some genes of <i>SNHG</i> family, while in tumor suppression mainly include <i>H22954, NEAT1, SNHG4, LINC01128</i> , etc. This article reviews the role of lncRNAs in the occurrence and development of AML, as well as those related to AML resistance and prognosis assessment, so as to provide a theoretical basis for the diagnosis and prognosis analysis of AML.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Role of Notch Signaling Pathway in Adult Patients with Epstein-Barr Virus-induced Infectious Mononucleosis].","authors":"Yu Li, Lian-Xiang Li, Ying Gao","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.041","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.041","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the changes of Notch signaling molecules and Th22 cells in adult patients with infectious mononucleosis (IM), and assess the regulatory function of Notch signaling inhibition to Th22 cells.</p><p><strong>Methods: </strong>Forty-two IM patients and twenty-one healthy controls were enrolled in this study. Their peripheral blood was collected, from which plasma and peripheral blood mononuclear cells (PBMCs) were isolated. Plasma interleukin (IL)-17 and IL-22 were measured by enzyme-linked immunosorbent assay. The percentages of CD3⁺ CD4⁺ IL-17⁺ Th17 cells and CD3⁺ CD4⁺ IL-22⁺ Th22 cells were investigated by flow cytometry. The mRNA relative levels corresponding to Th17 transcription factor retinoic acid related orphan receptor γt (RORγt), Th22 transcription factor aryl hydrocarbon receptor (AhR), and Notch signaling pathway molecules (including Notch receptors, Notch ligands, Notch downstream molecules) were semi-quantified by real-time PCR. CD4⁺ T cells were purified and stimulated with γ-secretase inhibitor (GSI). Cellular proliferation, Th17 and Th22 percentage, IL-17 and IL-22 secretion, transcription factor mRNA were measured in response to GSI stimulation.</p><p><strong>Results: </strong>The relative expression levels of <i>Notch1</i> and <i>Notch2</i> mRNA in PBMCs of IM group were 13.58±3.18 and 4.73±1.16, respectively, which were significantly higher than 1.09±0.12 and 1.07±0.15 in PBMCs of control group (both <i>P</i> < 0.001). However, there were no significant differences in relative expression levels of <i>Notch3</i> and <i>Notch4</i> mRNA between IM group and control group (<i>P</i> >0.05). The relative expression levels of Notch ligands (including <i>DLL1</i> and <i>Jagged1</i> ) mRNA and Notch downstream molecules (including <i>Hes1, Hes5,</i> and <i>Hey1</i> ) were increased in IM group compared with control group (all <i>P</i> < 0.001). In IM group, the Th17 and Th22 percentage were 5.03%±1.15% and 4.48%±1.29%, respectively, which were both higher than 4.36%±0.82% and 3.83%±0.55% in control group (both <i>P</i> < 0.05). In IM group, the IL-17 and IL-22 level were (301.1±53.82) and (101.2±16.45) pg/ml, respectively, which were both higher than (237.2±72.18) and (84.75±11.83) pg/ml in control group (both <i>P</i> < 0.001). In IM group, the relative expression levels of <i>RORγt</i> and <i>AhR</i> mRNA were 1.25±0.22 and 1.21±0.12, respectively, which were both higher than 0.99±0.15 and 1.04±0.11 in control group (both <i>P</i> < 0.001). There were no remarkable differences in CD4⁺ T cell proliferation, Th17 percentage, IL-17 secretion, and relative expression level of <i>RORγt</i> mRNA between cells with GSI stimulation and without GSI stimulation (<i>P</i> >0.05). GSI stimulation reduced Th22 percentage, IL-22 secretion, and relative expression level of <i>AhR</i> mRNA compared with non-stimulation (a","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular Diagnosis and Pedigree Analysis of Rare Mutations in Non-coding Region of <i>HBA2</i> Gene].","authors":"Li-Zhu Chen, Ti-Zhen Yan, Jun Huang, Qing-Yan Zhong, Xue Qin, Ning Tang, Shi-Qiang Luo","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.044","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.03.044","url":null,"abstract":"<p><strong>Objective: </strong>To perform molecular diagnosis and pedigree analysis for one case with α-thalassemia who does not conform to the genetic laws, and explore the effects of a newly discovered rare mutation (<i>HBA2:c.*12G>A</i>) on clinical phenotypes.</p><p><strong>Methods: </strong>Blood samples of the proband and her family members were collected for blood routine analysis, and the hemoglobin components were analyzed by capillary electrophoresis. The common α- and β-globin gene loci in Chinese population were detected by conventional techniques (Gap-PCR, RDB-PCR). The α-globin gene sequences (<i>HBA1, HBA2</i>) were analyzed by Sanger sequencing.</p><p><strong>Results: </strong>By analyzing the test results of proband and her family members, the genotype of the proband was -α^3.7/<i>HBA2:c.*12G>A</i>, her father was <i>HBA2:c.*12G>A</i> heterozygous mutation carrier.</p><p><strong>Conclusion: </strong>This study identifies a rare α-globin gene mutation (<i>HBA2:c.*12G>A</i>) that has not been reported before. It is found that heterozygous mutation carriers present with static α-thalassemia.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Role of Telomere in Clonal Evolution of Acquired Aplastic Anemia--Review].","authors":"Dan-Ni Li, Xian-Hao Wen","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.048","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.03.048","url":null,"abstract":"<p><p>Studies have found that 1/3 patients with acquired aplastic anemia have shortened telomere length, and the shorter the telomere, the longer the disease course, the more prone to relapse, the lower the overall survival rate, and the higher the probability of clonal evolution. The regulation of telomere length is affected by many factors, including telomerase activity, telomerase-related genes, telomere regulatory proteins and other related factors. Telomere shortening can lead to genetic instability and increases the probability of clonal evolution in patients with acquired aplastic anemia. This article reviews the role of telomere in the clonal evolution of acquired aplastic anemia and factors affecting telomere length.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pedigree Analysis of Hereditary Coagulation Factor XII Deficiency Caused by Compound Heterozygous Mutation p.Gly175Cys and p.Gly542Ser of <i>F12</i> Gene].","authors":"Xiao-Li Cheng, Ting Yang, Liu Yang, Yi-Juan Xin, Mu He, Lin Zhu, Jia-Yun Liu","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.033","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.03.033","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the clinical phenotype and gene mutation of a genetic coagulation factor XII (FXII) deficiency pedigree and explore the molecular pathogenesis.</p><p><strong>Methods: </strong>The activated partial thromboplastin time (APTT) and FXII activity (FXII:C) were detected by clotting method. The FXII antigen (FXII:Ag) was tested with ELISA. All exons and flanks of <i>F12</i> gene were determined by Sanger sequencing. ClustalX-2.1-win, PROVEAN and Swiss-Pdb Viewer software were used to analyze the conservatism of amino acids at the mutant site, forecast whether the mutant amino acids were harmful and confirm the influence of the mutation on protein structure.</p><p><strong>Results: </strong>The APTT of the proband prolonged to 71.3 s. The FXII:C and FXII:Ag were decreased to 5% and 6%, respectively. There were two heterozygous missense mutations c.580G>T and c.1681G>A detected in exon 7 and exon 14 of <i>F12</i> gene, resulting in p.Gly175Cys and p.Gly542Ser, severally. Proband's father carried the p.Gly175Cys heterozygous mutation, while mother, brother and daughter had the p.Gly542Ser heterozygous mutation. Software analysis showed that both Gly175 and Gly542 were conserved, the two mutations were harmful and when mutations had occurred, the corresponding sites affected the protein local structure.</p><p><strong>Conclusion: </strong>The p.Gly175Cys and p.Gly542Ser compound heterozygous mutations are the molecular pathogenesis of the hereditary coagulation FXII deficiency pedigree. The p.Gly175Cys mutation has been detected for the first time in the world.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Effecacy and Safety of Daratumumab Based Regimens in Relapsed/Refractory Multiple Myeloma: A Single-Center Real-World Data Analysis].","authors":"Han-Yan Zeng, Zhi-Juan Lin, Zhi-Feng Li, Long Liu, Man-Man Deng, Bing Xu","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.016","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.016","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the efficacy and safety of daratumumab based regimens in relapse and/or refractory multiple myeloma (RRMM) in the real world, as well as the impact of daratumumab on stem cell collection and engraftment.</p><p><strong>Methods: </strong>The clinical data of patients with RRMM who received daratumumab in hematology department of the First Affiliated Hospital of Xiamen University from February 2019 to March 2023 and had evaluable efficacy were retrospective analysis.</p><p><strong>Results: </strong>All 43 RRMM patients were treated with daratumumab-based combination regimens, including Dd, DVd, DRd, Dkd, DId, and Dara-DECP. With median follow-up time 10.1 (2.1-36.6) months, the best overall response rate (ORR) was 74.4% and a best complete response rate (CR) was 25.6%. 1-year overall survival rate (OS) was 84.5%. The most common severe hematologic adverse events (Grade>3) are 3/4 grade leukopenia（18.6%）, and the most common severe non-hematologic adverse events were infusion-related reactions (IRRs， 20.9%) and infections（7.0%）. Multivariate prognostic analysis showed that extramedullary infiltration was an independent adverse prognostic factor affecting OS （<i>P</i> =0.004）. The use of daratumumab has no effect on stem cell collection, or engraftment.</p><p><strong>Conclusion: </strong>Daratumumab is safe and effective in RRMM.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of Risk Factors for Pulmonary Infection in Patients with Acute Leukemia after Chemotherapy].","authors":"Xue-Peng Zhang, Ya-Ming Xi","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.043","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.043","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the risk factors of pulmonary infection in patients with acute leukemia (AL) after chemotherapy.</p><p><strong>Methods: </strong>A total of 294 patients with AL were collected and divided into infection group (<i>n</i>=93) and control group (<i>n</i>=201) according to whether the pulmonary infection occurred after chemotherapy. Analyze the correlation between sociodemographic data (sex, age, BMI), clinical data (disease type, ECOG score, invasive procedure, underlying disease, hormone therapy, empirical use of antibiotics, prognosis stratification, chemotherapy intensity, primitive cell count, white blood cell count, neutrophil count, duration of granulocyte deficiency, platelet count, hemoglobin, and albumin and pulmonary infection after chemotherapy. COX regression method was used to analyze the risk factors of pulmonary infection in AL patients after chemotherapy.</p><p><strong>Results: </strong>Among 294 patients with AL, 11 died within 30 days after pulmonary infection. There were statistically significant differences in age, smoking history, ECOG score, invasive procedure, hormone therapy, empirical use of antibiotics, prognosis stratification, chemotherapy intensity, primitive cell count, neutrophil count, duration of granulocyte deficiency, platelet count, hemoglobin, albumin and fasting blood glucose between the 2 groups (<i>P</i> <0.05). COX regression analysis showed that smoking history, invasive procedure, unexperienced use of antibiotics, poor prognosis, long duration of granulocytopenia, low platelet level and low albumin were high risk factors for pulmonary infection in AL patients after chemotherapy (<i>P</i> <0.05).</p><p><strong>Conclusion: </strong>Smoking, invasive procedures, unexperienced use of antibiotics, poor prognosis, long duration of granulodeficiency, low platelet levels and low albumin are risk factors for pulmonary infection in AL patients after chemotherapy.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of <i>UVRAG</i> Gene on Ferroptosis Induced by Sorafenib in K562 Cells].","authors":"Yan-Min Ma, Yan Wang, Min Yang, Ze-Min Cai, Xiao-Cheng Yin","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.001","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.03.001","url":null,"abstract":"<p><strong>Objective: </strong>To explore the effect of UV radiation resistance-associated gene (UVRAG) on ferroptosis induced by sorafenib in leukemia K562 cells.</p><p><strong>Methods: </strong>K562 cells were treated with 0, 0.625, 1.25, 2.5, 5, 10, and 20 μmol/L sorafenib for 24 or 48 hours, and the cell viability was detected by CCK-8 assay. Flow cytometry technology was used to detect the changes of reactive oxygen species (ROS) in K562 cells treated with 0, 5, and 10 μmol/L sorafenib for 24 hours. Western blot was used to detect the protein expression of GPX4 in K562 cells treated with 0, 5, and 10 μmol/L sorafenib and pretreatment with ferroptosis inhibitor. A recombinant lentiviral vector was used to construct <i>UVRAG</i> overexpression cell line in K562 cells. qPCR and Western blot were used to verify <i>UVRAG</i> gene overexpression, and Western blot detected the effect of <i>UVRAG</i> on the protein expression of GPX4 and HMGB1 after treatment with sorafenib.</p><p><strong>Results: </strong>Different concentrations of sorafenib could significantly inhibit the proliferation of K562 cells, and the cell viability gradually decreased with the increase of concentration (<i>r</i> _{24 h}=-0.9841, <i>r</i> _{48 h}=-0.9970). The level of ROS was increased (When the concentration was 10 μmol/L, <i>P</i> <0.001), while the expression of GPX4 protein was decreased in the process of 0, 5, 10 μmol/L sorafenib-induced K562 cell death (<i>P</i> <0.05), and the decrease in GPX4 protein could be partially reversed by pretreatment with ferroptosis inhibitor (<i>P</i> <0.05). Compared with NC group and NC-Sorafenib group, the expression of GPX4 protein was significantly decreased (both <i>P</i> <0.05), while HMGB1 protein was significantly increased (both <i>P</i> <0.05).</p><p><strong>Conclusion: </strong>Sorafenib can induce ferroptosis in K562 cells, and this process can be promoted by <i>UVRAG</i>.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of CD8⁺ CD28^- T Cells on Acute Graft-Versus-Host Disease after Haploidentical Hematopoietic Stem Cell Transplantation].","authors":"An-Di Zhang, Xiao-Xuan Wei, Jia-Yuan Guo, Xiang-Shu Jin, Lin-Lin Zhang, Fei Li, Zhen-Yang Gu, Jian Bo, Li-Ping Dou, Dai-Hong Liu, Meng Li, Chun-Ji Gao","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.038","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.03.038","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of CD8⁺ CD28^- T cells on acute graft-versus-host disease(aGVHD) after haploidentical hematopoietic stem cell transplantation(haplo-HSCT).</p><p><strong>Methods: </strong>The relationship between absolute count of CD8⁺ CD28^- T cells and aGVHD in 60 patients with malignant hematological diseases was retrospectively analyzed after haplo-HSCT, and the differences in the incidence rate of chronic graft-versus host disease(cGVHD), infection and prognosis between different CD8⁺ CD28^- T absolute cells count groups were compared.</p><p><strong>Results: </strong>aGVHD occurred in 40 of 60 patients after haplo-HSCT, with an incidence rate of 66.67%. The median occurrence time of aGVHD was 32.5(20-100) days. At 30 days after the transplantation, the absolute count of CD8⁺ CD28^- T cells of aGVHD group was significantly lower than that of non-aGVHD group (<i>P</i> =0.03). Thus the absolute count of CD8⁺ CD28^- T cells at 30 days after transplantation can be used to predict the occurrence of aGVHD to some extent. At 30 days after transplantation, the incidence rate of aGVHD in the low cell count group (CD8⁺ CD28^- T cells absolute count < 0.06/μl) was significantly higher than that in the high cell count group (CD8⁺ CD28^- T cells absolute count ≥0.06/μl,<i>P</i> =0.011). Multivariate Cox regression analysis further confirmed that the absolute count of CD8⁺ CD28^-T cells at 30 days after transplantation was an independent risk factor for aGVHD, and the risk of aGVHD in the low cell count group was 2.222 times higher than that in the high cell count group (<i>P</i> =0.015). The incidence of cGVHD, fungal infection, EBV infection and CMV infection were not significantly different between the two groups with different CD8⁺ CD28^- T cells absolute count. The overall survival, non-recurrent mortality and relapse rates were not significantly different between different CD8⁺ CD28^- T cells absolute count groups.</p><p><strong>Conclusion: </strong>Patients with delayed CD8⁺ CD28^- T cells reconstitution after haplo-HSCT are more likely to develop aGVHD, and the absolute count of CD8⁺ CD28^- T cells can be used to predict the incidence of aGVHD to some extent. The absolute count of CD8⁺ CD28^- T cells after haplo-HSCT was not associated with cGVHD, fungal infection, EBV infection, and CMV infection, and was also not significantly associated with the prognosis after transplantation.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}