中国实验血液学杂志最新文献

{"title":"[Diagnosis/Treatment Status and Research Progress of Primary Thyroid Lymphoma--Review].","authors":"Hao Sun, Fei Li","doi":"10.19746/j.cnki.issn1009-2137.2026.04.042","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.042","url":null,"abstract":"<p><strong>Objective: </strong>Primary thyroid lymphoma (PTL), a rare malignancy originating from thyroid lymphoid tissue, is strongly associated with Hashimoto's thyroiditis where accelerated lymphocyte proliferation and chronic antigen stimulation constitute key pathogenic mechanisms. The predominant pathological subtypes of PTL include diffuse large B-cell lymphoma and mucosa-associated lymphoid tissue lymphoma (MALTL). Current research on molecular alterations primarily focuses on MALTL, particularly pathogenic mutations in <i>CD274</i> and <i>TNFRSF14</i> . Diagnostic imaging modalities such as ultrasonography and computed tomography contribute significantly to PTL identification and differential diagnosis, while core needle biopsy remains the gold standard for definitive diagnosis. Therapeutic advancements demonstrate that combination therapies incorporating targeted agents with radiochemotherapy can improve clinical outcomes, with surgical intervention serving as a viable option for early-stage patients. This article reviews the latest research advances in PTL.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"1217-1221"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical Characteristics and Survival Analysis of Patients with IgD Multiple Myeloma].","authors":"Lin Gui, Fei Wang, Hui-Hui Song, Bao-An Chen","doi":"10.19746/j.cnki.issn1009-2137.2026.04.017","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.017","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical characteristics, survival status and prognostic factors of patients with IgD multiple myeloma (MM).</p><p><strong>Methods: </strong>The clinical data of 21 IgD MM patients with complete follow-up data who were treated at The Affiliated Zhongda Hospital of Southeast University and The Affiliated Jiangning Hospital of Nanjing Medical University from January 2018 to October 2024 were retrospectively analyzed. Their clinical characteristics, laboratory indicators, the efficacy of different maintenance therapy regimens (lenalidomide maintenance therapy vs. non-lenalidomide maintenance therapy), and prognostic influencing factors were analyzed. Overall survival (OS) and progression-free survival (PFS) were utilized as the primary prognostic evaluation indicators. Survival analysis was conducted using the Kaplan-Meier method, univariate analysis of prognostic factors was performed with the log-rank test, and multivariate analysis was carried out via the Cox proportional hazards regression model.</p><p><strong>Results: </strong>Among the 21 patients, 15 were male and 6 were female. By the follow-up cutoff date of January 31, 2025, 18 patients had died and 3 remained alive. Univariate analysis indicated that the presence of extramedullary plasmacytoma (EMP) at initial diagnosis, lenalidomide maintenance therapy, creatinine level, and minimal residual disease (MRD) status were associated with patients' OS; lenalidomide maintenance therapy, creatinine level, and MRD status were associated with PFS. Multivariate analysis revealed that lenalidomide maintenance therapy was an independent protective factor for OS and PFS in patients with IgD MM, while MRD⁺ was an independent risk factor for OS.</p><p><strong>Conclusion: </strong>IgD MM is associated with a high early mortality rate. Patients who achieve MRD negative demonstrate longer OS, and maintenance therapy regimens containing lenalidomide can significantly improve patient prognosis.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"1046-1052"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Intervention Factors and Outcome Analysis of Allogeneic Hematopoietic Stem Cell Transplantation in Elderly Patients with Acute Myeloid Leukemia].","authors":"Yu-Jie Ji, Zi-Yan Jin, Xiao Ma","doi":"10.19746/j.cnki.issn1009-2137.2026.04.009","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.009","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the outcomes and associated intervention factors in elderly patients with acute myeloid leukemia (AML) undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT).</p><p><strong>Methods: </strong>A retrospective analysis was performed on 67 AML patients aged≥60 years who received allo-HSCT in the First Affiliated Hospital of Soochow University and Soochow Hopes Hematonosis Hospital from June 2012 to September 2024. Based on HCT-CI score, the patients were divided into 0-1 group (36 cases), 2-3 group (20 cases) and≥4 group (11 cases), and the differences in survival, relapse, transplant-related mortality (TRM), disease-free survival (DFS), graft-versus-host disease (GVHD) and GVHD-free, relapse-free survival (GRFS) among the 3 groups were compared.</p><p><strong>Results: </strong>Among the 67 patients, the 1-year survival rate was 68.66%, 1-year relapse rate was 19.40%, 1-year TRM was 14.93%, and 1-year DFS rate was 67.16%. There were no significant differences in survival, relapse, TRM, DFS, GVHD and GRFS among HCT-CI 0-1, 2-3 and≥4 groups.</p><p><strong>Conclusion: </strong>Allo-HSCT has good outcomes for AML patients aged≥60 years. There was no significant correlation between HCT-CI score and survival outcome.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"993-999"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[XMU-MP-1 Enhances the Anti-Inflammatory Effects of Bone Marrow Mesenchymal Stem Cells in an Inflammatory Microenvironment by Regulating the Hippo Pathway].","authors":"Wan-Fu Jiang, Li Xu","doi":"10.19746/j.cnki.issn1009-2137.2026.04.040","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.040","url":null,"abstract":"<p><strong>Objective: </strong>To establish a bone marrow-derived inflammation model via in vitro induction and investigate the effect of XMU-MP-1 on enhancing the anti-inflammatory capacity of bone marrow mesenchymal stem cells (BMSCs) in an inflammatory microenvironment through regulating the Hippo signaling pathway.</p><p><strong>Methods: </strong>An <i>in vitro</i> inflammatory model was constructed by lipopolysaccharide (LPS) induction. BMSCs were randomly divided into three groups: control group, LPS group, and XMU-MP-1 intervention group. The inflammatory response of BMSCs was evaluated by detecting the expression levels of inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Alcian blue staining, alizarin red staining, and analysis of the expression of osteogenic and chondrogenic marker genes were performed to assess the differentiation potential of BMSCs. Meanwhile, the expression changes of Hippo pathway-related proteins were detected to explore the underlying mechanism.</p><p><strong>Results: </strong>Compared with the control group, LPS stimulation significantly increased the secretion levels of IL-6 and TNF-α in BMSCs by 13.7 times and 7.08 times, respectively (both <i>P</i><0.001), and reduced the number of osteogenic calcium nodules by 55.31% and the positive staining level of chondrogenic cartilage matrix by 30.67% (both <i>P</i><0.01), which significantly inhibited its osteogenic and chondrogenic differentiation abilities. After intervention with 100 nmol/L XMU-MP-1, the expression levels of IL-6 and TNF-α in BMSCs were decreased by 41.24% and 63.84% compared with the LPS group (both <i>P</i><0.01), while the 80 nmol/L intervention group decreased by 43.06% and 65.7% (both <i>P</i><0.01). The number of osteogenic calcium nodules in the 80 and 100 nmol/L XMU-MP-1 intervention groups increased by 54.42% and 98.01% compared with the LPS group, and the positive staining level of chondrogenic cartilage matrix increased by 11.41% and 21.14% (all <i>P</i><0.01), and the osteogenic and chondrogenic related indicators were significantly restored. Mechanistic studies showed that XMU-MP-1 could downregulate the phosphorylation level of key upstream proteins in the Hippo pathway, accompanied by increased YAP activity.</p><p><strong>Conclusion: </strong>XMU-MP-1 can improve the anti-inflammatory capacity and differentiation function of BMSCs in an inflammatory microenvironment to a certain extent, and its effect may be related to the regulation of the Hippo-YAP signaling pathway. This study provides experimental evidence for further exploring the regulatory mechanism of the bone marrow microenvironment under inflammatory conditions.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"1201-1209"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Impact of ABO Minor-Compatible Platelet Transfusion on the Efficacy of Transfusion in Patients Undergoing Allogeneic Hematopoietic Stem Cell Transplantation].","authors":"Chen-Xuan Zhang, Jie Xu, Wen Wang, Yuan-Ling Zuo, Ju-Ping Zhai","doi":"10.19746/j.cnki.issn1009-2137.2026.04.025","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.025","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the impact of long-term and repeated ABO mismatched (minor-compatible) platelet transfusion on the clinical transfusion efficacy in patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT), with the aim of providing a scientific and rational basis for clinical platelet transfusion strategies.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 674 hematological patients who received platelet transfusions following allo-HSCT in our hospital from June 2022 to May 2024. Among them, 390 patients (57.86%) received ABO-identical platelet transfusions, while 284 patients (42.14%) received ABO-mismatched platelet transfusions. Clinical efficacy was evaluated based on the change in platelet count 12 hours post-transfusion (△PLT), corrected count increment (CCI), incidence of clinical bleeding complications, anti-A/B antibody titer levels of the patient's plasma and engraftment status.</p><p><strong>Results: </strong>Although the CCI values in the ABO-mismatched platelet transfusion group were lower than those in the ABO-identical group (<i>P</i> < 0.001), there were no statistically significant differences in platelet transfusion frequency, total transfusion volume, incidence of bleeding complications during hospitalization, and duration of granulocyte recovery post-transplant (<i>P</i> > 0.05). Among the patients who received minor-compatible platelet transfusions, 106 (37.32%) patients who received mixed blood-type platelet transfusions showed significant differences in platelet transfusion frequency, total transfusion volume, and duration of granulocyte recovery post-transplant compared to 178 (62.68%) patients who received single blood-type platelet transfusions (all <i>P</i> < 0.05). A subgroup analysis based on the anti-A/B antibody titers in the plasma of ABO-incompatible allo-HSCT patients revealed that, regardless of whether the anti-A/B antibodies were of the IgG or IgM class, there were no significant differences in the total volume of platelet transfusions post-transplant across the different antibody titer groups (<i>P</i> >0.05).</p><p><strong>Conclusion: </strong>ABO-identical platelet transfusion significantly improves platelet recovery and survival in allo-HSCT patients. During long-term multiple transfusions, minor-compatible platelets exhibit comparable efficacy to ABO-identical platelets, however, when two compatible blood types are available, single-type platelet transfusion demonstrates superior efficacy.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"1106-1113"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prognostic Value of Serum Total Bilirubin in Lymphoma-Associated Hemophagocytic Lymphohistiocytosis].","authors":"Ping Liu, Jing-Xin Zhou, Yan-Ping Liu, Ahmed Shanza, Yi Miao, Lei Fan, Wen-Yu Shi, Jian-Yong Li","doi":"10.19746/j.cnki.issn1009-2137.2026.04.036","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.036","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the prognostic significance of serum total bilirubin (TBIL) in lymphoma-associated hemophagocytic lymphohistiocytosis (LA-HLH).</p><p><strong>Methods: </strong>The clinical data of 251 adult patients with LA-HLH admitted to the First Affiliated Hospital of Nanjing Medical University from January 2011 to April 2024 were retrospectively analyzed. According to the serum TBIL levels, the patients were stratified into a TBIL-normal group and a TBIL-elevated group. The differences in clinical parameters, 60-day survival rate, and median overall survival (OS) were compared between the two groups.</p><p><strong>Results: </strong>Compared to patients with normal TBIL, those with elevated TBIL had significantly higher levels of soluble CD25 (sCD25), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), along with lower levels of platelets (PLT) and fibrinogen (FIB) (all <i>P</i><0.05). Among patients with T/NK-cell non-Hodgkin lymphoma (T/NK-NHL)-associated HLH, those with elevated serum TBIL had a lower 60-day survival rate and a shorter median OS (both <i>P</i><0.05).</p><p><strong>Conclusion: </strong>In patients with LA-HLH, elevated serum TBIL is associated with decreased PLT and FIB levels, as well as increased levels of sCD25, AST and ALT. For patients with HLH secondary to T/NK-NHL, elevated serum TBIL indicates a poor prognosis.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"1178-1183"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expression Levels and Diagnostic Value of miR-138-5p and HIF-1α in the Serum of Newly Diagnosed Acute Myeloid Leukemia Patients].","authors":"Cong-Cong Zhang, Yin-Ling Li, Wei Chang","doi":"10.19746/j.cnki.issn1009-2137.2026.04.005","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.005","url":null,"abstract":"<p><strong>Objective: </strong>To explore the expression levels and diagnostic value of miR-138-5p and hypoxia-inducible factor-1α (HIF-1α) in newly diagnosed acute myeloid leukemia (AML) patients.</p><p><strong>Methods: </strong>Fifty newly diagnosed AML patients who were treated in our hospital from January 2020 to June 2023 were enrolled as study subjects. According to the severity of their condition, they were separated into low-risk group ( <i>n</i> =15), medium-risk group ( <i>n</i> =27), and high-risk group ( <i>n</i> =8). Additionally, 50 healthy individuals who underwent physical examinations during the same period were selected as the control group. The serum levels of miR-138-5p and HIF-1α were compared among different groups. Multivariate logistic regression analysis was applied to investigate the factors influencing the development of AML; Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of serum miR-138-5p and HIF-1α levels for AML.</p><p><strong>Results: </strong>The AML group exhibited significantly higher white blood cell (WBC) counts and HIF-1α levels, but lower miR-138-5p levels compared to the control group (all <i>P</i> < 0.001). Serum miR-138-5p levels showed a sequential decrease across the low-, intermediate-, and high-risk groups (<i>P</i> < 0.001), whereas the HIF-1α levels demonstrated a corresponding sequential increase (<i>P</i> < 0.001). Multivariate logistic regression analysis revealed that elevated miR-138-5p level was a protective factor against AML occurrence, while elevated WBC count and HIF-1α level were independent risk factors for AML development (all <i>P</i> < 0.05). ROC curve analysis showed that the combination of serum miR-138-5p and HIF-1α achieved an area under the curve (AUC) of 0.936 for diagnosing AML. This diagnostic performance was superior to that of serum miR-138-5p alone ( <i>Z</i>=2.135, <i>P</i> =0.033) or HIF-1α alone ( <i>Z</i>=2.666, <i>P</i> =0.008).</p><p><strong>Conclusion: </strong>Serum miR-138-5p expression is significantly downregulated, while HIF-1α expression is significantly upregulated in AML patients. The combination of both markers shows considerable diagnostic potential for AML.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"965-970"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High-Risk Factors of Multidrug-Resistant Organism Infection in Patients with Acute Leukemia and Construction of Nomogram Prediction Mode].","authors":"Xiao-Xia DU, Yan-Ni Bai","doi":"10.19746/j.cnki.issn1009-2137.2026.04.003","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.003","url":null,"abstract":"<p><strong>Objective: </strong>To explore the high-risk factors of multidrug-resistant organism (MDRO) infection in patients with acute leukemia, and to construct a nomogram prediction model.</p><p><strong>Methods: </strong>A total of 200 patients with acute leukemia admitted to the Department of Hematology, the First Affiliated Hospital of Air Force Military Medical University from January 2024 to January 2025 were enrolled in this study. The specimens of the enrolled were cultured. According to the presence or absence of MDRO infection, the patients were divided into the MDRO group (<i>n</i>=32) and the non-MDRO group (<i>n</i>=168). Clinical data of the two groups were collected and compared. Logistic regression analysis was used to analyze the influencing factors of MDRO infection in patients with acute leukemia. A nomogram model was constructed according to the selected influencing factors. The predictive accuracy of the model was evaluated by receiver operating characteristic (ROC) curve and calibration curve.</p><p><strong>Results: </strong>Of the 32 patients complicated with MDRO infection, a total of 35 pathogenic strains were isolated. The top three pathogens were methicillin-resistant coagulase-negative <i>staphylococci</i> (14 strains, 40.00%), <i>Klebsiella pneumoniae</i> (7 strains, 20.00%), and extended-spectrum β-lactamase-producing <i>Escherichia coli</i> (6 strains, 17.14%). Multivariate logistic regression analysis showed that indwelling drainage tube (<i>OR</i> =4.551), after hematopoietic stem cell transplantation (HSCT) (<i>OR</i> =3.216), use of antibiotics within 90 days of infection (<i>OR</i> =5.192), and diabetes mellitus (<i>OR</i> =5.083) were independent risk factors for MDRO infection in patients with acute leukemia (all <i>P</i> < 0.05). The ROC curve results under the nomogram model showed that the AUC was 0.736 (95%<i>CI</i> : 0.644-0.828), the sensitivity was 0.792, and the specificity was 0.739. The calibration curve and Hosmer-Lemeshow goodness-of-fit test showed <i>χ</i>²=0.973, <i>P</i> =0.615, indicating good fitting performance and favorable predictive accuracy of the model. The decision curve analysis demonstrated that the nomogram possessed high clinical utility in predicting the risk of MDRO infection in patients with acute leukemia.</p><p><strong>Conclusion: </strong>Indwelling drainage tube, use of antibiotics within 90 days after HSCT, and diabetes mellitus are risk factors for MDRO infection in patients with acute leukemia, and their predictive value is high.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"948-954"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of Multiparameter Flow Cytometry Combined with Serum Cys C and IL-10 in the Diagnosis of Multiple Myeloma].","authors":"Ju-Yan Yang, Zhen-Hong Luo, Guo Chen, Wei-Jin Liao, Yi-Fang Zhu","doi":"10.19746/j.cnki.issn1009-2137.2026.04.015","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.015","url":null,"abstract":"<p><strong>Objective: </strong>To explore the application of multiparameter flow cytometry combined with serum cystatin C (Cys C) and interleukin-10 (IL-10) in the diagnosis of multiple myeloma (MM).</p><p><strong>Methods: </strong>A total of 167 patients with suspected MM attending our hospital from March 2023 to July 2024 were enrolled, including 106 MM patients (MM group) and 61 non-MM patients (non-MM group). The patients underwent multiparameter flow cytometry and serum Cys C and IL-10 detection. The multivariate logistic regression analysis was conducted to identify the influencing factors of MM, and ROC curve analysis to evaluate the diagnostic value of multiparameter flow cytometry combined with serum Cys C and IL-10 for MM.</p><p><strong>Results: </strong>The ratio of plasma cells, CD56, the levels of serum Cys C and IL-10 in the MM group were significantly higher than those in the non-MM group, while the levels of CD19 and CD45 were lower (all <i>P</i> < 0.001). Multivariate logistic regression analysis showed that the ratio of plasma cells, Cys C, and IL-10 were risk factors for MM (<i>OR</i> =2.493, 3.843, 2.726, <i>P</i> < 0.05). ROC curve analysis indicated that the diagnostic value of multiparameter flow cytometry combined with serum Cys C and IL-10 for MM was higher than that of single diagnosis.</p><p><strong>Conclusion: </strong>The ratio of plasma cells and the levels of serum Cys C and IL-10 are elevated in MM patients, which lead to an increased risk of MM disease. The combination of the three has certain diagnostic value for MM.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"1031-1035"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[miR-212 Is Involved in AML Pathogenesis by Regulating <i>WT1</i> Expression Through Methylation Modification].","authors":"Jing Xu, Yu Duan, Jin-Juan Wang, Xiao-Hua Cui, Jian-Bing Liu, Jian-Qing Hao, Fang-Gang Ren","doi":"10.19746/j.cnki.issn1009-2137.2026.04.002","DOIUrl":"https://doi.org/10.19746/j.cnki.issn1009-2137.2026.04.002","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the underlying mechanism of <i>WT1</i> gene overexpression in patients with acute myeloid leukemia (AML) and to clarify the role of miR-212 in the pathogenesis of AML.</p><p><strong>Methods: </strong>Stem-loop reverse transcription was used to synthesize miR-212 cDNA, and RT-qPCR was used to detect the expression levels of miR-212 and <i>WT1</i> in bone marrow samples from <i>de novo</i> AML patients, bone marrow transplant donors, and patients with iron deficiency anemia (IDA). The correlation between miR-212 and <i>WT1</i> expression was further analyzed. A AML cell model with miR-212 overexpression was constructed by infecting Kasumi-1 cells with lentivirus. WT1 protein expression level was detected by Western blot, cell proliferation capacity was detected by CCK-8 assay, and cell cycle distribution and apoptosis were detected by flow cytometry. Dual-luciferase reporter gene assay was used to verify the direct targeted binding relationship between miR-212 and <i>WT1</i> . Bisulfite sequencing PCR (BSP) was used to analyze the methylation status of <i>MIR212</i> gene promoter.</p><p><strong>Results: </strong>The expression level of miR-212 in <i>de novo</i> AML patients was significantly lower than that in the control group, and it was negatively correlated with <i>WT1</i> mRNA expression (<i>r</i> =-0.77). Overexpression of miR-212 significantly decreased the expression of <i>WT1</i> mRNA and protein in Kasumi-1 cells, inhibited cell proliferation, reduced the proportion of cells in the S and G₂/M phases, and increased the proportion of early apoptotic cells. The dual-luciferase reporter gene assay revealed that the luciferase activity ratio in 293T cells cotransfected with the <i>WT1</i> wild-type vector and miR-212-3p mimic was significantly lower than that in the control group, indicating a direct targeted binding relationship between miR-212 and <i>WT1</i> mRNA. The sequencing results showed that the average methylation rate of CpG sites in the <i>MIR212</i> promoter region of Kasumi-1 cells was 96.7%.</p><p><strong>Conclusion: </strong>In AML, promoter hypermethylation leads to the downregulation of miR-212, and such downregulation relieves the targeted inhibition of <i>WT1</i> , resulting in the upregulation of <i>WT1</i> expression and thus contributing to the pathogenesis of AML. miR-212 may be a new therapeutic target for AML patients with high <i>WT1</i> expression.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 4","pages":"941-947"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}