中国实验血液学杂志最新文献

{"title":"[The Relationship between OPN, NLR and Chemotherapy Efficacy in Patients with Acute Myeloid Leukemia].","authors":"Jing-Jing Yu, Kai DU, Xiao-Dong Zhang","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.005","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.005","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the relationship between osteopontin (OPN), neutrophil/lymphocyte ratio (NLR) and chemotherapy efficacy in patients with acute myeloid leukemia (AML).</p><p><strong>Methods: </strong>The clinical data of AML patients who received cytarabine (Ara-C) + idarubicin (IDA) induction chemotherapy in Nanyang First People's Hospital from March 2021 to February 2023 were retrospectively analyzed through the hospital information system. According to the induction chemotherapy results, the selected patients were divided into chemotherapy effective and ineffective groups. Logistic regression was used to analyze the relationship between OPN, NLR and chemotherapy efficacy in AML patients. Receiver operating characteristic (ROC) curves and decision curves were drawn to analyze the application value of OPN and NLR in predicting chemotherapy efficacy in AML patients.</p><p><strong>Results: </strong>This study enrolled 103 AML patients, and 30 patients did not achieve complete response (CR) after two rounds of induction chemotherapy and were evaluated as chemotherapy ineffective (29.13%), with chemotherapy effective accounting for 70.87%. The proportion of patients aged≥60 years, with poor prognosis and combined central nervous system infiltration (CNSL) in the chemotherapy ineffective group were higher than those in the chemotherapy effective group, and serum OPN, lactate dehydrogenase (LDH), β₂-microglobulin (β₂-MG), and NLR values were also significantly higher than those in the chemotherapy effective group (all <i>P</i> < 0.01). Logistic regression analysis showed that the ineffective chemotherapy in AML patients was associated with age≥60 years, poor prognosis, increased NLR value, and high expression of serum OPN, LDH, and β₂-MG (all <i>P</i> < 0.05). ROC curve analysis showed that the area under the curve for OPN and NLR alone or in combination to predict the risk of ineffective chemotherapy in AML was 0.787, 0.770, and 0.853, respectively, all of which had certain predictive value. Decision curve analysis showed that age, prognostic risk, NLR, OPN, LDH, and β₂-MG all had certain benefits in predicting the risk of chemotherapy failure in AML patients, and the combined prediction had a higher benefit rate.</p><p><strong>Conclusion: </strong>The combination of Ara-C and IDA in AML patients has a risk of ineffective induction chemotherapy, which may be related to serum OPN and NLR values. High expression of serum OPN and elevated NLR value may increase the risk of ineffective induction chemotherapy. The combination of serum OPN and NLR can assist in improving the predictive value of Ara-C combined with IDA in AML patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"28-34"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular Biological Mechanism of Ael Novel Mutant Subtypes].","authors":"Yu Zhang, Ya-Ting Ling, Hai-Lin DU, Jie Cai, Qiang Fu, Cheng-Tao He","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.039","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.039","url":null,"abstract":"<p><strong>Objective: </strong>Genotyping was performed on a specimen whose blood type was inconsistent with positive and negative serological tests, and its family investigation and molecular biological mechanism were studied.</p><p><strong>Methods: </strong>Blood group serology was used to identify the ABO blood group of the proband and her family members. <i>ABO</i> gene of proband and her family samples were sequenced by <i>ABO</i> gene direct sequencing (Sanger sequencing) and SMRT haplotype sequencing methods. AlphaFold software was used to simulate the three-dimensional structure of proteins, and the protein structures before and after mutation were compared to observe the changes in the intermolecular forces within the structure.</p><p><strong>Results: </strong>Blood group serological test showed that the proband had Ael/B phenotype, her mother and daughter had Ael/O phenotype, and the blood group results of other family members were consistent with positive and negative stereotypes. The sequencing results showed that there was a new mutation of c.1024 A>C in exon 7 of <i>ABO</i> gene in the proband and her mother and daughter, which led to the mutation of amino acid No. 342 from threonine to proline (p.Thr342Pro). The AlphaFold software modeling results showed that the mutation of p.Thr342Pro significantly reduced the number of hydrogen bonds formed with 215-bit Glu.</p><p><strong>Conclusion: </strong><i>ABO</i> gene exon 7 c.1024 A>C mutation leads to amino acid replacement of polypeptide chain, affects the stability of GTA protein structure, causes changes in enzyme activity, produces Ael phenotype, and this variant gene can be stably inherited.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"266-271"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Observation of the Therapeutic Effect of Venetoclax Combined with HEA Regimen on Acute Myeloid Leukemia Patients with <i>KMT2A</i> Gene Rearrangement].","authors":"Shan-Dong Tao, Rong Wen, Jia-Xin Li, Xin-Qi Zheng, Wen-Ting Shi, Bang-He Ding, Liang Yu, Chun-Ling Wang","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.001","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.001","url":null,"abstract":"<p><strong>Objective: </strong>To observe the treatment response rate and safety of the combination of venetoclax and HEA (VHEA) regimen in the induction therapy of acute myeloid leukemia (AML) patients with <i>KMT2A</i> gene rearrangement.</p><p><strong>Methods: </strong>Six patients with AML accompanied by <i>KMT2A</i> gene rearrangement were treated with the VHEA regimen ［venetoclax 100 mg on day 1, 200 mg on day 2, 400 mg on day 3-14; Homoharringtonine 2 mg/(m²·d) on day 1-7; Etoposide 100 mg/d on day 1-5; Cytarabine 100 mg/(m²·d) on day 1-7］. The remission rate and safety of the VHEA regimen were observed.</p><p><strong>Results: </strong>Among the 6 patients, 3 were relapsed or refractory AML, and 3 were newly diagnosed patients. After one course of induction chemotherapy, 5 patients achieved complete remission (CR), with 3 cases showing minimal residual disease (MRD) < 1.0×10^-3 by flow cytometry and 3 cases showing 0.00% MRD by PCR, one patient who relapsed 13 months after transplantation died from pulmonary infection before evaluating the efficacy. Three patients underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) in CR1, one patient relapsed 12 months after transplantation, with a total survival time of 20 months. The other 2 patients are currently in disease-free survival, with a follow-up period of 15 months and 5 months, respectively. Two patients did not undergo allo-HSCT, one patient died from relapse 4 months after survival, and the other patient is currently in CR and undergoing maintenance chemotherapy. The total CR rate of VHEA regimen in 6 patients with <i>KMT2A</i> gene rearrangement AML was 83.3% (5/6), with no treatment-related deaths. Chemotherapy-related adverse reactions such as myelosuppression and infection were within controllable range.</p><p><strong>Conclusion: </strong>The VHEA regimen can significantly improve the CR rate of AML patients with <i>KMT2A</i> gene rearrangement, with good safety. It may be a better choice for induction remission chemotherapy in newly diagnosed and relapsed/refractory AML patients with <i>KMT2A</i> gene rearrangement.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of Clinical Characteristics and Prognosis in Patients with NCAM1-Positive Acute Myeloid Leukemia].","authors":"Zhong-Li Hu, Meng-Qing Hua, Jia-Jia Li, Zhong-Ting Hu, Yan-Li Yang","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.003","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.003","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the expression of NCAM1 in the bone marrow of patients with acute myeloid leukemia (AML), explore the clinical characteristics of NCAM1⁺AML patients, and investigate the impact of NCAM1 on the prognosis of AML patients.</p><p><strong>Methods: </strong>Flow cytometry was used to detect the immunophenotypes of 82 AML patients. The bone marrow smears of these patients were stained, and the nucleated cells were classified and counted. Real-time quantitative PCR was used to detect the relative expression of <i>WT1</i> . Comparative analysis was performed on the differences in tumor burden, blood routine tests, bone marrow cytology characteristics, and the expression of <i>WT1</i> gene between patients with NCAM1⁺AML and those with NCAM1^-AML. Additionally, the differences in the initial induction complete remission (CCR) rate and overall survival (OS) were compared between NCAM1⁺AML and NCAM1^-AML patients.</p><p><strong>Results: </strong>The average platelet count (PLT) of NCAM1⁺AML patients was significantly lower than that of NCAM1^-AML patients (<i>P</i> < 0.001). The percentage of residual nucleated red blood cells (<i>P</i> =0.028 9) and the number of megakaryocytes (<i>P</i> < 0.001) in the bone marrow of NCAM1⁺AML patients were significantly reduced compared to NCAM1^-AML patients. At the initial diagnosis, the expression level of <i>WT1</i> in the bone marrow of NCAM1⁺AML patients was significantly higher than that in NCAM1^-AML patients (<i>P</i> =0.037 8). There were no significant differences in tumor burden, peripheral white blood cells (WBC) and hemoglobin (Hb) levels, as well as the proportions of residual granulocytes, blast cells, POX positivity, and positive rates of Auer rods in the bone marrow between NCAM1⁺AML and NCAM1^-AML patients (<i>P</i> >0.05). The OS time of NCAM1⁺AML patients was significantly shorter than that of NCAM1^-AML patients (2.5 months <i>vs.</i> . 5 months, <i>P</i> =0.045 7). However, there was no significant difference in the initial induction CR rate between the two groups (<i>P</i> >0.05).</p><p><strong>Conclusion: </strong>NCAM1⁺AML has unique clinical characteristics. NCAM1 not only promotes the occurrence and drug resistance of leukemia, but may also affect the prognosis of AML patients by affecting bone marrow hematopoiesis.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"14-20"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Effect of Haplo-Cord Mixed Chimerism on Lymphocyte Subsets in Aplastic Anemia after Haplo-Cord HSCT].","authors":"Yu-Qing Gao, Ou Xu, Shao-Yan Hu","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.022","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.022","url":null,"abstract":"<p><strong>Objective: </strong>To explore the changes and clinical significance of lymphocyte subsets in peripheral blood of aplastic anemia (AA) pediatric patients with mixed chimerism of dual donors.</p><p><strong>Methods: </strong>Twenty-five pediatric patients with AA who underwent a haploidentical stem cells combined with umbilical cord blood transplantation (haplo-cord HSCT) in Children's Hospital of Soochow University from March 2020 to April 2023 were included. The changes in chimerism and lymphocyte subgroup levels after transplantation were observed. The differences in lymphocyte subgroups between haploid chimeric and double donor chimeric pediatric patients were compared.</p><p><strong>Results: </strong>After haplo-cord HSCT, the absolute count of NK cells in the 25 pediatric patients recovered and reached reference levels within as early as 3 months, the absolute count of CD8 ⁺ T cells reconstituted more rapidly compared to CD4 ⁺ T cells, while B cells hadn't reached reference level after 12 months. Among the 25 pediatric patients, 5 cases (20%) showed haplo-cord mixed chimerism and others were haploidentical chimerism. In the haploidentical chimerism group, the absolute count of B cells had significantly lower levels at 6 and 12 months after transplantation compared to the haplo-cord mixed chimerism group (both <i>P</i> < 0.05), and the absolute count of NK cell was significantly lower at 6 months after transplantation (<i>P</i> < 0.01).</p><p><strong>Conclusion: </strong>After haplo-cord HSCT, NK cells reconstitution is the fastest, while B cells reconstitution is the slowest. B cells reconstitute in the haplo-cord mixed chimerism group is faster compared to the haploidentical chimerism group.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"159-163"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of Ellagic Acid on the Growth and Platelet-Producing Function of Megakaryocytes after X-Ray Irradiation].","authors":"Kang Sun, Chun-Liang Liu, Fan-Bi Meng, Yan Yan, Zi-Yu Zhao, Ke-Sheng Dai","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.040","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.040","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of ellagic acid on the growth and platelet-producing function of megakaryocytes after X-ray irradiation, in order to clarify the therapeutic effect of ellagic acid on ionizing radiation-induced thrombocytopenia.</p><p><strong>Methods: </strong>In <i>in vitro</i> experiments, human primitive megakaryocytes CMK cells were treated with ellagic acid and irradiated with X-rays using a bio-irradiator. The proliferation of CMK cells was detected by CCK-8 assay. The apoptosis rate and reactive oxygen species (ROS) level of CMK cells were detected by flow cytometry. In <i>in vivo</i> experiments, C57BL/6J male mice were given gastric gavage with ellagic acid and irradiated with X-rays using a bio-irradiator. The number of megakaryocytes in the mouse bone marrow was measured by HE staining and immunohistochemistry. The apoptosis rate, ROS level and polyploid content of mouse bone marrow megakaryocytes were detected by flow cytometry, and the counts of platelets in the peripheral blood of mice were detected by a hemocytometer after X-rays irradiation.</p><p><strong>Results: </strong><i>In vitro</i> experiments showed that ellagic acid treatment at different concentrations (1, 2, 5, 10, and 20 μmol/L) could increase the proliferation rate of CMK cells after X-ray irradiation (5 μmol/L showing the best effect), decrease the content of intracellular ROS levels, but did not affect the apoptosis rate.<i>in vivo</i> experiments showed that gavage of ellagic acid at a concentration of 20 mg/kg could delay the decrease of platelet number in the peripheral blood of X-ray irradiated mice and promote recovery, increase the number and the polyploid content of megakaryocytes in the bone marrow, and reduce the ROS content, but did not affect the apoptosis rate.</p><p><strong>Conclusion: </strong>Ellagic acid can play a therapeutic role in radiation-induced thrombocytopenia by increasing the number and platelet-producing capacity of megakaryocytes <i>in vivo</i> and <i>ex vivo</i> after X-ray irradiation, which may act by reducing ROS produced by ionizing radiation.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"272-278"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of the Degree of Myelofibrosis on the Therapeutic Efficacy and Survival in Patients with Myelodysplastic Syndromes].","authors":"Rui-Ping Li, Yan-Ping Ma","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.020","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.020","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the effect of the degree of myelofibrosis (MF) on clinical characteristics, therapeutic efficacy of hypomethylating agents (HMA) and survival in patients with myelodysplastic syndromes (MDS).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The clinical data of 103 patients with newly diagnosed MDS who received HMA treatment in the Department of Hematology of the Second Hospital of Shanxi Medical University from January 1, 2016 to July 15, 2021 were retrospectively analyzed. The patients were divided into three groups according to the degree of fibrosis in bone marrow biopsy: MDS-MF0, MDS-MF1, and MDS-MF2/3. The clinical characteristics, efficacy and survival were compared among three groups. Logistic regression and Cox regression analyses were employed to identify factors affecting the efficacy and prognosis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Among 103 MDS patients, 60 cases (58.2%) had no myelofibrosis (MDS-MF0), 28 cases (27.2%) had mild myelofibrosis (MDS-MF1), and 15 cases (14.6%) had moderate to severe myelofibrosis (MDS-MF2/3). The median hemoglobin (Hb) of patients in the MDS-MF0, MDS-MF1, and MDS-MF2/3 groups showed statistical differences (&lt;i&gt;P&lt;/i&gt;=0.016), and the proportion of patients with -5/del(5q) also showed statistical differences among the three groups (&lt;i&gt;P&lt;/i&gt;=0.046). A total of 97 patients were included in the efficacy analysis, with an overall response rate (ORR) of 62.9%. The ORRs of the MDS-MF0, MDS-MF1, and MDS-MF2/3 groups were 72.2%, 53.6% and 46.7%, respectively, with no statistically significant difference (&lt;i&gt;P&lt;/i&gt;=0.093). The ORR in MDS patients combined with MF was 51.2%, which was statistically significantly different from that in MDS-MF0 patients (&lt;i&gt;P&lt;/i&gt;=0.033). Multivariate analysis showed that more than 4 cycles of treatment was an independent favorable factor affecting the overall response to HMA therapy (&lt;i&gt;OR&lt;/i&gt;=7.532, 95%&lt;i&gt;CI&lt;/i&gt;: 2.031-27.938, &lt;i&gt;P&lt;/i&gt;=0.003), while the degree of myelofibrosis did not affect the efficacy (&lt;i&gt;P&lt;/i&gt;&gt;0.05). By the end of follow-up, among 101 patients included in the survival analysis, 27 cases (26.7%) transformed into leukemia, a total of 75 cases (74.3%) died, 19 cases (18.8%) remained alive, and 7 cases (6.9%) were lost to fllow-up. The median overall survival (OS) was 21.4 months, the median progression-free survival (PFS) was 10.3 months, and the median leukemia-free survival (LFS) was 18.1 months. In the MDS-MF0, MDS-MF1, and MDS-MF2/3 groups, the median OS was 27.9, 12.2 and 11 months (&lt;i&gt;P&lt;/i&gt;=0.017), respectively; the median PFS was 13.5, 9.1 and 6.4 months (&lt;i&gt;P&lt;/i&gt;=0.025), respectively; and the median LFS was 24.5, 11 and 11 months (&lt;i&gt;P&lt;/i&gt;=0.020), respectively. For MDS patients with low blast (MDS-LB), there were statistically significant differences in OS and LFS between those with and without MF (OS: &lt;i&gt;P&lt;/i&gt;=0.024, LFS: &lt;i&gt;P&lt;/i&gt;=0.026), while no significant difference was observed in PFS (&lt;i&gt;P&lt;/i&gt;=0.057). Multivariate analysis sho","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"142-153"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[NK Cell Immunotherapy for Acute Myeloid Leukemia: Recent Advances and the Immunomodulatory Role of Traditional Chinese Medicine--Review].","authors":"Shu-Peng Chen, Jie Liu, Kai-Yue Xia, Ying-Jian Zeng","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.042","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.042","url":null,"abstract":"<p><p>Acute myeloid leukemia (AML) is a hematological malignancy caused by abnormal proliferation and blocked maturation of hematopoietic stem cells, leading to the accumulation of a large number of immature or abnormal myeloid cells in the bone marrow and blood. Immune escape is one of the core mechanisms underlying the refractory and relapse of AML. Natural killer (NK) cells play a crucial role in immune surveillance and immune killing. In recent years, NK cell-based immunotherapy has experienced rapid development, especially in allogeneic NK cell therapy, chimeric antigen receptor NK (CAR-NK) cell therapy, intelligent NK cell therapy, and combination strategies with other treatments, providing new therapeutic hope for relapsed and refractory AML patients. Traditional Chinese medicine (TCM) also plays a regulatory role in natural killer (NK) cell function. For instance, acupuncture therapy, Chinese herbal compounds, and herbal extracts can enhance the quantity and activity of NK cells, thereby promoting their potential antitumor effects. This review aims to summarize the latest developments in NK cell therapy for AML and provide scientific references for precise treatment strategies of AML.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"288-292"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147474833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research Progress of Megakaryocyte Morphology in the Prognosis of Primary Myelofibrosis--Review].","authors":"Zhi-Kang Zheng, Jian Huang","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.043","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.043","url":null,"abstract":"<p><p>Primary myelofibrosis (PMF) is a myeloproliferative neoplasm (MPN) characterized by malignancy derived from hematopoietic stem cells. Compared with polycythemia vera (PV) and essential thrombocythemia (ET), PMF shows a worse clinical prognosis. Therefore, it is essential to explore biological markers for early identification and intervention to delay the process of the disease. Megakaryocytes (MK) play a central role in the pathogenesis and disease progression of PMF. The thrombophilia, the aggravation of myelofibrosis and the transformation to acute myeloid leukemia (AML) in patients with PMF are closely related to the morphological characteristics of MK.Changes in MK morphology are not only regulated by inflammatory mediators, but also influenced by specific genetic factors. This article will review the mechanism of MK morphological changes in PMF and the latest research progress on the prognosis of PMF with MK morphology, so as to provide reference for early clinical diagnosis and treatment.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"293-297"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of Anti-HCV Confirmatory Test and Nucleic Acid Quantitative Test Results in Blood Center Laboratory].","authors":"Ya-Zi Zhao, Xian-Yuan Wang, Xue-Feng Han, Hui-Xian Zhang, Wei Han, Yan-Bin Wang","doi":"10.19746/j.cnki.issn.1009-2137.2026.01.027","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.027","url":null,"abstract":"<p><strong>Objective: </strong>To understand the situation of Hepatitis C infection among voluntary blood donors, and to provide decision-making basis for the screening and control of Hepatitis C in blood collection and supply institutions.</p><p><strong>Methods: </strong>The unqualified anti-HCV ELISA samples from voluntary blood donors in our center from 2020 to 2023 were selected, and the results of western blot (WB) test and nucleic acid quantitative test were compared and analyzed.</p><p><strong>Results: </strong>Among the 444 samples for WB test, WB results were positive in 276 cases (62.16%), uncertain in 53 cases (11.94%) and negative in 115 cases (25.90%). Among the positive results of WB, 58 cases (21.01%) were full band type, there were 58 cases (21.01%) with 4 bands, there were 45 cases (16.30%) with 3 bands, there were 80 cases with 2 bands (28.99%), only Core bands were found in 35 cases (12.68%). Among the positive results of WB, the positive rate of Core was 96.01%, followed by NS3-1 (80.07%), NS3-2 (51.09%), NS4 (40.94%) and NS5 (40.58%), the positive rates of different bands were statistically significant (<i>P</i> <0.01). 143 WB positive results and nucleic acid quantitative results were compared and analyzed, there was a statistically significant difference in the detection rate of different band types of nucleic acid <i>P</i> <0.01). The quantitative detection rates of Core, NS3-1, NS3-2, NS4 and NS5 in the positive group were higher than those in the negative group, and the differences were statistically significant. Multivariate Logistic regression analysis showed that NS3-2(<i>OR</i>=15.171，95%<i>CI</i>:3.720-61.874，<i>P</i> <0.01) and NS5(<i>OR</i>=4.511，95%<i>CI</i>:1.228-16.573， <i>P</i> <0.05) were independent risk factors for HCV nucleic acid detection after adjusting other variables.</p><p><strong>Conclusion: </strong>Analysis of the characteristics of WB positive bands can provide additional information for assessing the virus replication status. NS3-2 and NS5 status can be used as important reference indicators to predict the virus replication, suggesting the risk of infection in blood donors, assisting blood collection and supply institutions to make screening decisions and optimize the detection process.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"34 1","pages":"192-197"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}