中国实验血液学杂志最新文献

{"title":"[The Comparison of Efficacy and Safety between Venetoclax Combined with Demethylating Drugs and the \"3+7\" Drug Regimen for Acute Myeloid Leukemia].","authors":"Na Cui, Li-Jing Wang, Chang-Yong Yuan","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.004","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.004","url":null,"abstract":"<p><strong>Objective: </strong>To explore the efficacy and safety of venetoclax combined with demethylating drugs and intense chemotherapy in the treatment of acute myeloid leukemia (AML).</p><p><strong>Methods: </strong>The clinical data of 76 patients with AML treated in Qilu Hospital of Shandong University Dezhou Hospital from January 2019 to March 2024 were retrospectively analyzed. Patients were divided into observation group and control group. 38 patients in the observation group received venetoclax combined with demethylating drugs (decitabine or azacytidine) and 38 patients in the control group with the \"3+7\" intensive chemotherapy regimen. The primary endpoints of clinical observation were complete remission (CR), CR with incomplete hematologic recovery (CRi), partial remission (PR), non remission (NR), and overall response rate (ORR). Secondary endpoints were overall survival (OS) and drug safety.</p><p><strong>Results: </strong>After 2 courses of treatment, the CR+CRi rate in observation group and control group was 71.05% and 65.79%, respectively, and the ORR was 81.58% and 78.95%, respectively. After all courses of treatment, CR+CRi rate in the observation group and the control group was 73.68% and 78.95%, respectively, and the ORR was 81.58% and 84.21%, respectively, with no statistical significance between the two groups (<i>P</i> >0.05). After 1 course of treatment, there were statistically significant differences in the proportion and degree of myelosuppression, the duration of neutropenia and the duration of thrombocytopenia between the two groups (<i>P</i> < 0.05), while there were no statistically significant differences in the occurrence of neutropenia with fever between the two groups (<i>P</i> >0.05). The incidence of non-hematological adverse reactions was highest in infection (mainly pulmonary infection) and gastrointestinal reaction. Among the many adverse reactions, there were statistically significant differences in the infection and hypokalemia between the two groups (<i>P</i> < 0.05), the incidence of hypokalemia in observation group and control group was 42.11% and 15.79%, respectively, and the infection rate in observation group and control group was 73.68% and 94.74%, respectively. The median OS was 13.13(1.67-53.63) months in the observation group and 16.60(0.57-59.67) months in the control group.</p><p><strong>Conclusion: </strong>The combination of venetoclax and demethylating drugs has a low degree of myelosuppression, but a long recovery time, a response rate as good as that of intensive chemotherapy, and a lower infection rate. However, the incidence of hypokalemia is low in the intensive chemotherapy regimen, and the regimen significantly improves the long-term outcome of patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"25-31"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[5-HT Promotes Proliferation and Inhibits Apoptosis of Megakarycytes through 5-HT2BR].","authors":"Hui-Min Kong, Yu-Rong Cen, Mo Yang, Qiang Peng, Jin-Qi Huang","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.011","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.011","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of 5-hydroxytryptamine (5-HT) on the proliferation, apoptosis and colony-forming unit-megakaryocyte (CFU-MK) of Meg-01 cells and its possible mechanisms.</p><p><strong>Methods: </strong>The uptake and metabolism of 5-HT in Meg-01 cells were analysed by reverse-phase high-performance liquid chromatography (RP-HPLC) with electrochemical detection. The expression of 5-HT2B receptor (5-HT2BR) in megakaryocytes was detected by immunofluorescence staining. The cell proliferation and viability were measured by MTT and Trypan blue staining after Meg-01 cells were single-cultured or co-cultured with different concentrations of 5-HT/5-HT2BR inhibitor Ketanserin for 48 h. Meg-01 cells were incubated with 5-HT/ Ketanserin for 72 h, then the flow cytometry was used to detect early apoptosis of the cells and the activity of caspase-3. Using CFU-MK assay to investigate the effect of 5-HT on the differentiation of megakaryocytes.</p><p><strong>Results: </strong>5-HT could be uptaken by Meg-01 cells, and metabolized into 5-hydroxyindoleacetic acid (5-HIAA). The expression of 5-HT2BR on megakaryocytes could be detected after immunofluorescence staining. 5-HT could promote the proliferation of Meg-01 cells at a dose-dependent manner (<i>r</i> =0.82), with the most significant effect observed at a concentration of 200 nmol/L (<i>P</i> < 0.001). Trypan blue staining also indicated that 200 nmol/L 5-HT had the most significant effect on the viability of Meg-01 cells (<i>P</i> < 0.05). The proliferation of Meg-01 cells treated with 5-HT was increased compared with the untreated control (<i>P</i> < 0.001), while the combination of 5-HT with ketanserin downregulated this effect. 5-HT significantly reduced the early apoptosis rate (<i>P</i> < 0.001) and caspase-3 activity (<i>P</i> < 0.05) of Meg-01 cells, while addition of ketanserin significantly increased the early apoptosis rate of Meg-01 cells (<i>P</i> < 0.001) and caspase-3 activity also increased to some extent. 5-HT promoted the formation of CFU-MK in bone marrow cells in a dose-dependent manner (<i>r</i> =0.89). The addition of ketanserin reduced the promoting effect of 5-HT on CFU-MK formation (<i>P</i> < 0.01).</p><p><strong>Conclusion: </strong>There may be monoamine oxidase present in megakaryocytes, which can metabolize and decompose 5-HT into 5-HIAA. 5-HT may promote the proliferation and differentiation of megakaryocytes through 5-HT2BR. Besides, 5-HT can also reduce the apoptosis of megakaryocytes, and its anti-apoptotic effect may be mediated by 5-HT2BR and caspase-3 pathways.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Sequence Analysis and Confirmation of an HLA Null Allele Generated by a Base Insertion].","authors":"Zhan-Rou Quan, Yan-Ping Zhong, Liu-Mei He, Bing-Na Yang, Hong-Yan Zou","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.041","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.041","url":null,"abstract":"<p><strong>Objective: </strong>To confirm the sequence of a null allele <i>HLA-C*08:127N</i> produced by a base insertion.</p><p><strong>Methods: </strong>PCR sequence-specific oligonucleotide probe (SSOP) and PCR sequence-based typing (SBT) were used for HLA routine detection, which discovered abnormal sequence maps of <i>HLA-C</i> in one acute myeloid leukemia patient. The sequence of the above loci was confirmed by next generation sequencing (NGS) technology.</p><p><strong>Results: </strong>The SSOP typing result showed that <i>HLA-C</i> locus was <i>C*03:04, C*08:01</i>, while the sequence was suspected to be inserted or deleted in exon 3 by SBT, and finally confirmed by NGS as <i>C*03:04, C*08:127N</i>.</p><p><strong>Conclusion: </strong>When base insertion produces HLA null alleles, SBT analysis software cannot provide correct results, but NGS technology can more intuitively obtain accurate HLA typing results.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"276-279"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical Characteristics and Prognosis Analysis of Patients with Extranasal NK/T-Cell Lymphoma: A Multicenter Retrospective Study of Huaihai Lymphoma Working Group].","authors":"Hui-Rong Shan, Qing Zhang, Ling Wang, Yu-Ye Shi, Yu-Qing Miao, Tai-Gang Zhu, Jing-Jing Ye, Xu-Dong Zhang, Liang Wang, Zi-Yuan Shen, Wei Sang","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.013","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.013","url":null,"abstract":"<p><strong>Objective: </strong>To explore the clinical characteristics and prognostic factors of patients with extranasal NK/T-cell lymphoma (NKTCL).</p><p><strong>Methods: </strong>The clinical data of 138 patients with NKTCL diagnosed in 10 medical centers of Huaihai Lymphoma Working Group from June 2015 to April 2021 were collected and analyzed retrospectively. The differences in clinicopathological characteristics of patients with different involvement and efficacy of pegaspargase regimen were compared, as well as perform survival analysis.</p><p><strong>Results: </strong>A total of 138 extranasal NKTCL patients were included, with a median age of 46 years, and the ratio of males to females was approximately 2∶1. There were 39 patients with gastrointestinal involvement, 32 patients with oropharyngeal involvement, 17 patients with skin involvement, 11 patients with lymph node involvement, 11 patients with orbital involvement, and 28 patients with other parts involvement. Patients with skin involvement had a higher proportion of advanced disease and a lower proportion of CD56 positive rate compared to those with oropharyngeal involvement. Among the patients with gastrointestinal involvement, the survival rate of patients who received pegaspargase regimen was significantly higher than those who were treated without pegaspargase (<i>P</i> < 0.01). Multivariate analysis showed that serum creatinine was an independent prognostic factor for patients with skin involvement ( <i>HR</i> =1.027, 95%<i>CI</i> : 1.001-1.054, <i>P</i> =0.040), ECOG PS and EBV DNA were independent prognostic factors for patients with gastrointestinal involvement ( <i>HR</i> =2.635, 95%<i>CI</i> : 1.096-6.338, <i>P</i> =0.030; <i>HR</i> =4.772, 95% <i>CI</i> : 1.092-20.854, <i>P</i> =0.038), and ECOG PS and CA stage were independent prognostic factors for patients with oropharyngeal involvement ( <i>HR</i> =13.875, 95%<i>CI</i> : 2.517-76.496, <i>P</i> =0.002; <i>HR</i> =20.261, 95%<i>CI</i> : 2.466-166.470, <i>P</i> =0.005).</p><p><strong>Conclusion: </strong>The clinicopathological characteristics of extranasal NKTCL patients with different sites of involvement are vary, and effective individualized treatment need to be further explored.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"93-100"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expression and Biological Function of <i>SPOP</i> in Acute Myeloid Leukemia].","authors":"Xue-Ying Wan, Jing Xu, Xiao-Li Liu, Hong-Wei Wang","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.005","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.005","url":null,"abstract":"<p><strong>Objective: </strong>To study the expression of <i>SPOP</i> in patients with acute myeloid leukemia (AML) and its effect on proliferation, apoptosis and cycle of AML cells.</p><p><strong>Methods: </strong>RT-qPCR was used to detect the expression of <i>SPOP</i> mRNA in bone marrow samples of patients with newly diagnosed AML and normal controls. The stable overexpression of <i>SPOP</i> in AML cell lines THP-1 and U937 were constructed by liposome transfection. The effect of <i>SPOP</i> on cell proliferation was detected by CCK-8, and the effect of <i>SPOP</i> on apoptosis and cell cycle was detected by flow cytometry. The expressions of anti-apoptotic protein Bcl-2 and apoptotic protein Bax, Caspase3 were detected by Western blot.</p><p><strong>Results: </strong>The median expression level of <i>SPOP</i> mRNA in normal control group was 0.993 1(0.6303, 1.433), while that in AML group was 0.522 1(0.242 2, 0.723 7). The expression level of <i>SPOP</i> in AML group was significantly lower than that in normal control group ( <i>P</i> < 0.001). After the overexpression of <i>SPOP</i>, the proportion of apoptotic cells in the U937 overexpression group and THP-1 overexpression group was 10.9%±0.3% and 4.6%±015%, which were higher than 8.9%±0.3% and 3.0%±0.30% in the Empty Vector group, respectively (both <i>P</i> < 0.05). The expression of Caspase3 in U937 overexpression group and THP-1 overexpression group was 1.154±0.086 and 1.2±0.077, which were higher than 1 in Empty Vector group, respectively (both <i>P</i> < 0.05). The ratio of Bax/Bcl-2 in U937 overexpression group and THP-1 overexpression group was 1.328±0.057 and 1.669±0.15, which were higher than 1 in Empty Vector group, respectively (both <i>P</i> < 0.05). In the cell proliferation experiment, the number of cells in the U937 overexpression group and THP-1 overexpression group were both slightly lower than those in the Empty Vector group, but the differences were not statistically significant (<i>P</i> >0.05). In the cell cycle experiment, the proportion of G₁ cells in the U937 overexpression group and THP-1 overexpression group were both slightly higher than those in the Empty Vector group, but the differences were not statistically significant (<i>P</i> >0.05).</p><p><strong>Conclusion: </strong><i>SPOP</i> can promote the apoptosis of leukemic cells, and its mechanism may be related to down-regulation of Bcl-2 expression and up-regulation of Bax and Caspase3 expression.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"32-38"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Advances in Study of Erythroblastic Island Macrophages--Review].","authors":"Zhao-Hui Wang, Shuang Ding","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.044","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.044","url":null,"abstract":"<p><p>Bone marrow microenvironment is the environment in which hematopoietic stem cells live, mainly composed of bone marrow stromal cells, microvessels, nerves, and cytokines secreted by stromal cells. The bone marrow microenvironment plays a crucial role in the self-renewal, directed differentiation and proliferation of hematopoietic stem cells and the regulation of proliferation, differentiation and maturation of hematopoietic cells. A class of macrophages exists in the bone marrow microenvironment, the bone marrow-resident tissue macrophages, which plays a crucial role in maintaining homeostasis <i>in vivo</i>, and three subpopulations of bone marrow-resident tissue macrophages have been characterized: erythroblastic island macrophages (EIMs), hematopoietic stem cell niche macrophages, and bone macrophages. This review focuses on the functions, surface markers and modeling of EIMs.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"292-295"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical and Laboratory Characteristics of Streptococcus mitis Causing Bloodstream Infection in Children with Hematological Disease].","authors":"Yu-Long Fan, Guo-Qing Zhu, Zhi-Ying Tian, Yan-Xia Lyu, Zhao Wang, Ye Guo, Wen-Yu Yang, Qing-Song Lin, Xiao-Juan Chen","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.043","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.043","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the risk factors, clinical characteristics, and bacterial resistance of bloodstream infections caused by <i>Streptococcus mitis</i> in children with hematological disease, so as to provide a reference for infection control.</p><p><strong>Methods: </strong>The clinical information and laboratory findings of pediatric patients complicated with blood cultures positive for <i>Streptococcus mitis</i> from January 2018 to December 2020 in the Institute of Hematology & Blood Diseases Hospital were searched and collected. The clinical characteristics, susceptibility factors, and antibiotic resistance of the children were retrospectively analyzed.</p><p><strong>Results: </strong>Data analysis from 2018 to 2020 showed that the proportion of <i>Streptococcus mitis</i> isolated from bloodstream infections in children (≤14 years old) with hematological diseases was the highest (19.91%) and significantly higher than other bacteria, accounting for 38.64% of Gram-positive cocci, and presented as an increasing trend year by year. A total of 427 children tested positive blood cultures, including 85 children with bloodstream infections caused by <i>Streptococcus mitis</i> who tested after fever. Most children experienced a recurrent high fever in the early and middle stages (≤6 d) of neutropenia and persistent fever for more than 3 days. After adjusting the antibiotics according to the preliminary drug susceptibility results, the body temperature of most children (63.5%) returned to normal within 4 days. The 85 children were mainly diagnosed with acute myeloid leukemia (AML), accounting for 84.7%. The proportion of children in the neutropenia stage was 97.7%. The incidence of oral mucosal damage, lung infection, and gastrointestinal injury symptoms was 40%, 31.8%, and 27.1%, respectively. The ratio of elevated C-reactive protein (CRP) and procalcitonin was 65.9% and 9.4%, respectively. All isolated strains of <i>Streptococcus mitis</i> were not resistant to vancomycin and linezolid, and the resistance rate to penicillin, cefotaxime, levofloxacin, and quinupristin-dalfopristin was 10.6%, 8.2%, 9.4%, and 14.1%, respectively. None of children died due to bloodstream infection caused by <i>Streptococcus mitis</i>.</p><p><strong>Conclusion: </strong>The infection rate of <i>Streptococcus mitis</i> is increasing year by year in children with hematological diseases, especially in children with AML. Among them, neutropenia and oral mucosal damage after chemotherapy are high-risk infection factors. The common clinical symptoms include persistent high fever, oral mucosal damage, and elevated CRP. Penicillin and cephalosporins have good sensitivity. Linezolid, as a highly sensitive antibiotic, can effectively control infection and shorten the course of disease.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"286-291"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of Iron Overload on Expression of PD-1 on Surface of T Lymphocyte in Mice].","authors":"Yu-Mei Liu, Hua-Quan Wang, Zong-Hong Shao","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.039","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.039","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of iron overload on the expression of programmed death-1 (PD-1) on the surface of T lymphocyte in mice, in order to analyze the mechanism of iron overload inhibiting T cell function.</p><p><strong>Methods: </strong>Flow cytometry was used to detect the labile iron pool (LIP), reactive oxygen species (ROS), and the expression of PD-1 in peripheral blood T cells in mice with iron overload.</p><p><strong>Results: </strong>The mean fluorescence intensity of calcein in T cells of mice in iron overload group was 2 492±311.1, which was significantly lower than 3 136±537.3 in the control group ( <i>P</i> <0.01), suggesting that increased LIP in iron overload group. Compared with the control group, the ratio of CD4/CD8 of peripheral blood T cells was normal or increased in iron overload group. The level of ROS in T cells was 2 452±393.3 in iron overload group, which was significantly increased compared to 1 874±121.8 in the control group ( <i>P</i> <0.001). The expression of PD-1 on the surface of T cells was significantly increased. The percentage of PD-1⁺ cells in CD8⁺T cells was (12.97±6.92)% and (6.18±2.95)% in iron overload group and control group, respectively ( <i>P</i> <0.05), and that in CD8^-T cells was (33.55±15.69)% and (12.51±4.11)% ( <i>P</i> <0.001).</p><p><strong>Conclusion: </strong>The expression of PD-1 on peripheral blood T cells in mice with iron overload is significantly increased, which may be involved in inhibiting T cell effector function.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"262-268"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Enhancing Effects and Underlying Mechanism of Ionizing Radiation on Adipogenic Differentiation of Mesenchymal Stem Cells via Regulating Oxidative Stress Pathway].","authors":"Fu-Hao Yu, Bo-Feng Yin, Pei-Lin Li, Xiao-Tong Li, Jia-Yi Tian, Run-Xiang Xu, Jie Tang, Xiao-Yu Zhang, Wen-Jing Zhang, Heng Zhu, Li Ding","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.037","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2025.01.037","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects and underlying mechanism of ionizing radiation on the adipogenic of mesenchymal stem cells (MSCs).</p><p><strong>Methods: </strong>Mouse MSCs were cultured in vitro and treated with 2 Gy and 6 Gy radiation with ⁶⁰Co, and the radiation dose rate was 0.98 Gy/min. Bulk RNA-seq was performed on control and irradiated MSCs. The changes of adipogenic differentiation and oxidative stress pathways of MSC were revealed by bioinformatics analysis. Oil Red O staining was used to detect the adipogenic differentiation ability of MSCs <i>in vitro</i>, and real-time fluorescence quantitative PCR (qPCR) was used to detect the expression differences of key regulatory factors <i>Cebpa, Lpl</i> and <i>Pparg</i> after radiation treatment. At the same time, qPCR and Western blot were used to detect the effect of inhibition of <i>Nrf2</i>, a key factor of antioxidant stress pathway, on the expression of key regulatory factors of adipogenesis. Moreover, the species conservation of the irradiation response of human bone marrow MSCs and mouse MSC was determined by qPCR.</p><p><strong>Results: </strong>Bulk RNA-seq suggested that ionizing radiation promotes adipogenic differentiation of MSCs and up-regulation of oxidative stress-related genes and pathways. The results of Oil Red O staining and qPCR showed that ionizing radiation promoted the adipogenesis of MSCs, with high expression of <i>Cebpa, Lpl</i> and <i>Pparg</i>, as well as oxidative stress-related gene <i>Nrf2</i>. <i>Nrf2</i> pathway inhibitors could further enhance the adipogenesis of MSCs in bone marrow after radiation. Notably, the similar regulation of oxidative pathways and enhanced adipogenesis post irradiation were observed in human bone marrow MSCs. In addition, irradiation exposure led to up-regulated mRNA expression of interleukin-6 and down-regulated mRNA expression of colony stimulating factor 2 in human bone marrow MSCs.</p><p><strong>Conclusion: </strong>Ionizing radiation promotes adipogenesis of MSCs in mice, and oxidative stress pathway participates in this effect, blocking <i>Nrf2</i> further promotes the adipogenesis of MSCs. Additionally, irradiation activates oxidative pathways and promotes adipogenic differentiation of human bone marrow MSCs.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"246-254"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical Characteristics and Prognosis Analysis of Angioimmunoblastic T-Cell Lymphoma].","authors":"Hong-Bo Wang, Xiu-Juan Wang, Xin-Hong Guo","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.015","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.015","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical characteristics and prognostic factors of patients with angioimmunoblastic T-cell lymphoma (AITL).</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the clinical data of 42 AITL patients who were diagnosed and monitored in the Hematologic Disease Center of the First Affiliated Hospital of Xinjiang Medical University from 2011 to 2021. Kaplan-Meier method was used to calculate overall survival (OS) rate and progression-free survival (PFS) rate, while log-rank method was utilized for comparison.</p><p><strong>Results: </strong>The majority of 42 patients were elderly, with a median age at diagnosis of 66(22-80) years old, and 25 cases were males. There were 38 patients in Ann Arbor stage III-IV, 23 patients with B symptoms, and 34 patients with intermediate-high to high risk of International Prognostic Index (IPI) score. The most common clinical manifestation was painless lymph node swelling (31 cases), which could be accompanied by multiple serous effusion (24 cases), fever (17 cases), rash (11 cases), and anemia (15 cases). There were 18 patients with positive Epstein-Barr virus (EBV), 28 patients with Ki-67 ≥40%, and 6 patients accompanied by EBV viremia. The median survival time of the 42 patients was 12(1-121) months, 3-year OS rate was 37.6%, and 3-year PFS rate was 26.1%. Univariate analysis indicated that patients with elevated interleukin (IL)-6 and multiple serous effusion exhibited lower survival rate.</p><p><strong>Conclusion: </strong>AITL is characterized by diverse clinical manifestations, high aggressiveness and poor prognosis. Conventional chemotherapy is not effective. Elevated IL-6 and multiple serous effusion are poor prognostic factors for AITL patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"108-113"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}