中国实验血液学杂志最新文献

{"title":"[Genetic Variation of <i>SH2B3</i> in Patients with Myeloid Neoplasms].","authors":"Qiang Ma, Rong-Hua Hu, Hong Zhao, Xiao-Xi Lan, Yi-Xian Guo, Xiao-Li Chang, Wan-Ling Sun, Li Su, Wu-Han Hui","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.032","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.04.032","url":null,"abstract":"<p><strong>Objective: </strong>To observe the genetic variation of <i>SH2B3</i> in patients with myeloid neoplasms.</p><p><strong>Methods: </strong>The results of targeted DNA sequencing associated with myeloid neoplasms in the Department of Hematology, Xuanwu Hospital, Capital Medical University from November 2017 to November 2022 were retrospectively analyzed, and the patients with <i>SH2B3</i> gene mutations were identified. The demographic and clinical data of these patients were collected, and characteristics of <i>SH2B3</i> gene mutation, co-mutated genes and their correlations with diseases were analyzed.</p><p><strong>Results: </strong>The sequencing results were obtained from 1 005 patients, in which 19 patients were detected with <i>SH2B3</i> gene mutation, including 18 missense mutations (94.74%), 1 nonsense mutation (5.26%), and 10 patients with co-mutated genes (52.63%). Variant allele frequency (VAF) ranged from 0.03 to 0.66. The highest frequency mutation was p.Ile568Thr (5/19, 26.32%), with an average VAF of 0.49, involving 1 case of MDS/MPN-RS (with <i>SF3B1</i> mutation), 1 case of MDS-U (with <i>SF3B1</i> mutation), 1 case of aplastic anemia with PNH clone (with <i>PIGA</i> and <i>KMT2A</i> mutations), 2 cases of MDS-MLD (1 case with <i>SETBP1</i> mutation). The other mutations included p.Ala567Thr in 2 cases (10.53%), p.Arg566Trp, p.Glu533Lys, p.Met437Arg, p.Arg425Cys, p.Glu314Lys, p.Arg308*, p.Gln294Glu, p.Arg282Gln, p.Arg175Gln, p.Gly86Cys, p.His55Asn and p.Gln54Pro in 1 case each.</p><p><strong>Conclusion: </strong>A wide distribution of genetic mutation sites and low recurrence of <i>SH2B3</i> is observed in myeloid neoplasms, among of them, p.Ile568Thr mutation is detected with a higher incidence and often coexists with characteristic mutations of other diseases.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prognostic Value of DTA Mutations in Patients with Newly Diagnosed Acute Myeloid Leukemia].","authors":"Hui-Juan Chen, Yang Cao, Ying-Jie Miao, Yi-Fang Zhou, Yue Liu, Wei-Ying Gu","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.003","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.04.003","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the prognostic significance of DTA (<i>DNMT3A, TET2, ASXL1</i> ) gene mutations in patients with non-M3 acute myeloid leukemia (AML).</p><p><strong>Methods: </strong>The clinical data of 180 newly diagnosed AML patients hospitalized in the First People's Hospital of Changzhou from January 2018 to April 2022 were retrospectively analyzed. Next-generation sequencing technology was used to detect 150 gene mutations in the patients, and log-rank tests and Cox regression models were used to analyze the prognostic factors.</p><p><strong>Results: </strong>DTA gene mutations were detected in 83 (46.1%) of 180 AML patients. Compared to patients without DTA mutations, patients with DTA mutations were significantly older (<i>P</i> < 0.001). The median overall survival (OS) time and disease-free survival (DFS) time in the DTA mutation group were significantly shorter than those in the group without DTA mutation (both <i>P</i> < 0.05). Multivariate analysis showed that age ≥ 60 years (<i>P</i> < 0.001), with DTA mutation (<i>P</i> =0.018), and intermediate-risk (relative to favorable-risk) (<i>P</i> =0.005) were independent risk factors for OS in AML patients.</p><p><strong>Conclusion: </strong>AML patients with DTA mutations are relatively older, with shorter median OS time and DFS time, and poor prognosis.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of Thalassemia Gene Mutation Types and Ethnic Distribution Characteristics in Hechi Area, Guangxi].","authors":"Li-Fang Liang, Xiu-Ning Huang, Dong-Ming Li, Bi-Yan Chen, Xiang Chen, Zhen-Ren Peng, Sheng He","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.033","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.04.033","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the genotype, mutation type, and ethnic distribution characteristics of thalassemia in the population of Hechi area, Guangxi, and to provide a reference basis for prevention and control of thalassemia and eugenic counseling in the region.</p><p><strong>Methods: </strong>Gap-polymerase chain reaction (gap-PCR) and reverse dot blot (RDB) were used for genetic testing on suspected thalassemia persons, and the results were analyzed.</p><p><strong>Results: </strong>Among 29 136 samples, a total of 17 016 (58.40%) positive samples for thalassemia genes were detected, with a higher detection rate in males than in females (χ²=49.917，<i>P</i> < 0.001). The detection rates of thalassemia genes were significant different among Zhuang, Han, Yao, Mulao, and Maonan ethnic groups (χ²=546.121, <i>P</i> < 0.001). The α-thalassemia genotypes were mainly --^<i>SEA</i> /αα (16.67%), -α^<i>3.7</i>/αα (8.90%), α ^<i>CS</i>α/αα (6.00%). Additionally, four rare genotypes were detected, including -- ^<i>THAI</i>/αα (47 cases), HKαα/αα (2 cases), --^<i>SEA</i> /-α ^<i>21.9</i> (2 cases), and -- ^<i>THAI</i>/α^<i>CS</i>α (1 case). The β-thalassemia genotypes were mainly β ^<i>CD17</i>/β^<i>N</i> (7.49%), β^{<i>CD41-42</i>}/β^<i>N</i> (6.70%), β^{<i>CD71-72</i>}/β^<i>N</i> (0.44%). 108 cases of moderate and severe β-thalassemia were detected, of which 81 cases had a history of blood transfusion, the transfusion frequency of 60 cases was more than 10 times/year, and 10 cases received bone marrow transplantation.</p><p><strong>Conclusion: </strong>Thalassemia in Hechi area is predominantly deletion type --^<i>SEA</i> /αα, the detection rate of thalassemia in ethnic minorities is higher than that in Han population. In this area, moderate and severe β-thalassemia have certain incidence, these patients mostly need regular blood transfusion and iron removal treatment, and very few patients have received bone marrow transplantation. This study provides a certain reference basis for prevention and control of thalassemia and eugenic counseling in the region.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of Selinexor on Proliferation and Apoptosis of Acute Myeloid Leukemia Kasumi-1 Cells].","authors":"Lu-Hui Lin, Sun-Qiao Gao, Xu-Qiao Mei, Da-Yi Lin, Yi-Feng Chen, Su-Dan Lin, Li-Hong Zhuang, Cong-Meng Lin","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.017","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.04.017","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of selinexor, a inhibitor of nuclear export protein 1 (XPO1) on the proliferation inhibition and apoptosis of Kasumi-1 cells in acute myeloid leukemia (AML).</p><p><strong>Methods: </strong>MTS method was used to detect the inhibitory effect of different concentrations of selinexor on the proliferation of Kasumi-1 cells at different time points. The apoptosis rate and cell cycle changes after treatment with different concentration of selinexor were detected by flow cytometry.</p><p><strong>Results: </strong>Selinexor inhibited the growth of Kasumi-1 cells at different time points in a concentration-dependent manner (<i>r</i> _{24 h}=0.7592, <i>r</i> _{48 h}=0.9456, and <i>r</i> _{72 h}=0.9425). Selinexor inhibited Kasumi-1 cells growth in a time-dependent manner (<i>r</i> =0.9057 in 2.5 μmol/L group, <i>r</i> =0.9897 in 5 μmol/L group and <i>r</i> =0.9994 in 10 μmol/L group). Selinexor could induce apoptosis of Kasumi-1 cells in a dose-dependent manner (<i>r</i> =0.9732), and the apoptosis of Kasumi-1 cells was more obvious with the increase of drug concentration. The proportion of G₀/G₁ phase was significantly increased and the proportion of S phase was significantly decreased after the treatment of Kasumi-1 cells by selinexor. With the increase of drug concentration, the proportion of Kasumi-1 cells cycle arrest in G₀/G₁ phase was increased and the cell synthesis was decreased.</p><p><strong>Conclusion: </strong>Selinexor can promote the death of tumor cells by inhibiting Kasumi-1 cells proliferation, inducing apoptosis and blocking cell cycle.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Characteristic Analysis of Adult Acute Myeloid Leukemia Patients with <i>PTPN11</i> Gene Mutation].","authors":"Li Sheng, Ya-Jiao Liu, Jing-Fen Zhou, Hong-Ying Chao, Hai-Ying Hua, Xin Zhou, Xiao-Hong Zhao","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.014","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.04.014","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the incidence of <i>PTPN11</i> gene mutation and its associated gene mutations in adult patients with acute myeloid leukemia (AML), and analyze its clinical characteristics.</p><p><strong>Methods: </strong>Second-generation sequencing and Sanger sequencing were used to detect 51 gene mutations, and multiplex-PCR was used to detect 41 fusion genes from 451 newly diagnosed adult AML patients admitted to Affiliated Hospital of Jiangnan University, Changzhou Second People's Hospital, Wuxi People's Hospital and Wuxi Second People's Hospital from January 2017 to July 2022.</p><p><strong>Results: </strong>Among 451 primary adult AML patients, the <i>PTPN11</i> gene mutation was detected in 34 cases, and the mutation rate was 7.5%. In the 34 patients, 37 <i>PTPN11</i> alterations were found, which were exclusively missense mutations affecting residues located within the N-SH2 (31 cases) and PTP (6 cases) domains and clustered overwhelmingly in exon 3. The platelet count of <i>PTPN11</i> mutation patients was 76.5(23.5, 119.0)×10⁹/L, which was significantly higher than 41.0(22.0, 82.5)×10⁹/L of wild-type patients (<i>P</i> < 0.05). While, there were no significant differences in sex, age, peripheral white blood cell count, hemoglobin, and bone marrow blast between <i>PTPN11</i> mutation and wild-type patients (<i>P</i> >0.05). In FAB subtypes, <i>PTPN11</i> mutations were mainly distributed in M5, followed by M2 and M4, less frequently in M3 and M6. There was no significant difference in the distribution of FAB subtypes between <i>PTPN11</i> mutation and wild-type patients (<i>P</i> >0.05). A total of 118 AML patients were detected positive fusion gene, among which patients with <i>PTPN11</i> mutations had a higher incidence of positive MLL-AF6 than wild-type ones (<i>P</i> < 0.01). 97.1% of 34 patients with <i>PTPN11</i> mutations were accompanied by other mutations, in descending order, they were respectively <i>NPM1</i> (38.2%), <i>NRAS</i> (32.4%), <i>FLT3-ITD</i> (32.4%), <i>DNMT3A</i> (32.4%) and <i>KRAS</i> (23.5%), etc .</p><p><strong>Conclusion: </strong><i>PTPN11</i> mutation has a certain incidence in AML patients and is clustered overwhelmingly in exon 3. ALL of them are exclusively missense mutations, and most often present in conjunction with <i>NPM1</i> mutations. FAB typing of <i>PTPN11</i> mutation is mostly manifested as M5 subtype, which is associated with higher platelet counts.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficacy and Safety of Reduced Dose Azacitidine in the Treatment of Elderly Patients with Myelodysplastic Syndromes].","authors":"Cong Zhang, Cai Sun, De-Zhen Wang, Zhan-Wei Liu, Ting Fang","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.028","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.04.028","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the efficacy and safety of low-dose azacitidine in the treatment of senile myelodysplastic syndromes (MDS).</p><p><strong>Methods: </strong>A total of 92 elderly MDS patients who were initially diagnosed in the Huaibei Miners General Hospital and the Affiliated Hospital of Xuzhou Medical University from January 2018 to June 2022 were randomly divided into the observation group and the control group with 46 patients in each group. The observation group received a low dose of azacitidine 100 mg/d, d1-7, 28 days as a course of treatment, 6 courses in total, and the control group received a standard dose of azacitidine 75 mg(m²·d), d1-7, 28 days as a course of treatment, a total of 6 courses of treatment. The clinical efficacy, overall survival (OS) and adverse reactions of the two groups of patients were observed.</p><p><strong>Results: </strong>There was no statistically significant difference in the clinical data between the two groups (<i>P</i> >0.05). After treatment, the hemoglobin and platelet levels of the two groups of patients were significantly higher than before treatment in each group (<i>P</i> < 0.05). There was no statistically significant difference in leukocyte, hemoglobin and platelet levels between patients in the observation group and control group (<i>P</i> >0.05). The number of cases with complete remission, partial remission, hematological remission, disease stabilization and disease progression in the observation group were 4, 10, 22, 6 and 4, respectively, with a total effective rate of 78.26%. The numbers of cases with complete remission, partial remission, hematological remission, disease stabilization and disease progression in control group were 8, 12, 18, 4 and 4, respectively, with a total effective rate of 82.61%. The total effective rate of patients in the observation group was slightly lower than that of the control group(χ²=0.457, <i>P</i> =0.254). There was no significant difference between the two treatment schemes in the treatment of patients with blood transfusion dependence and patients with low risk, medium risk and high risk (<i>P</i> >0.05). It takes 4 and 6 courses of treatment to achieve the best treatment response in the control group and observation group respectively. There was no significant difference in OS between the two groups (<i>P</i> >0.05). In the observation group, there were 4, 6 and 2 cases of infection, III-IV degree myelosuppression and gastrointestinal reaction, respectively, with the incidence rate of adverse events being 26.09%. In the control group, there were 6, 16 and 6 cases of infection, III-IV degree myelosuppression and gastrointestinal reaction, respectively, with the adverse event rate was 60.87%. The incidence of adverse events in the control group was significantly higher than that in the observation group (χ²=7.095, <i>P</i> =0.036).</p><p><strong>Conclusion: </strong>Elderly patients with MDS have po","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical Characteristics and Prognosis of Myelodysplastic Syndromes Patients with <i>RUNX1</i> Gene Mutation].","authors":"Yi Chen, Yue-Ru Ji, Jing-Yi Zhang, Wei-Wei Qin, Cang-Chun Liu, Li Liu, Xue-Qian Yan","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.030","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.04.030","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical characteristics and survival analysis of myelodysplastic syndromes (MDS) with <i>RUNX1</i> gene mutation.</p><p><strong>Methods: </strong>Clinical data of 177 newly diagnosed MDS patients admitted to the Department of Hematology, the Second Affiliated Hospital of Air Force Military Medical University from October 1, 2015 to October 31, 2022 were retrospectively analyzed. Gene mutation detection was performed by second-generation sequencing technology, and clinical characteristics and prognosis of patients with <i>RUNX1</i> gene mutation were analyzed.</p><p><strong>Results: </strong>A total of 30 cases (16.95%) of <i>RUNX1</i> gene mutations were detected, including 15 missense mutations (50.0%), 9 frameshift deletion mutations (30.0%), 4 splice site mutations (13.3%), 1 insertion mutation (3.3%), and 1 nonsense mutation (3.3%). Patients with <i>RUNX1</i> mutations had a median age of 68.5 years at diagnosis (range: 62.25-78.50 years old). There were no significantly differences between <i>RUNX1</i> mutations and wild type patients in age distribution, gender, peripheral blood white blood cell count, hemoglobin level, bone marrow and peripheral blood blasts ratio, IPSS-R cytogenetics, IPSS-R stage, etc. (<i>P</i> >0.05). However, there were statistically significant differences in platelet count and whether complicated karyotype. Compared with patients without <i>RUNX1</i> gene mutation, patients with <i>RUNX1</i> gene mutation had lower platelet count (<i>P</i> =0.018), and were less likely to have complicatedkaryotype at initial diagnosis (<i>P</i> =0.01). Cox proportional hazards model analysis showed that when other covariates remained unchanged, the higher the platelet count, the better the survival of patients (<i>HR</i>=0.995, 95%<i>CI</i> : 0.990-0.999, <i>P</i> =0.036); In the IPSS-M prognostic stratification, keeping other covariates unchanged, the risk of progression or death of myelodysplastic syndrome was significantly lower in the medium to high-risk and low-risk groups compared with the high-risk group (<i>HR</i>=0.149, 95%<i>CI</i> : 0.031-0.721, <i>P</i> =0.018; <i>HR</i>=0.026, 95%<i>CI</i> : 0.003-0.234, <i>P</i> =0.001). Survival analysis showed that MDS patients with <i>RUNX1</i> gene mutation had worse overall survival time (<i>P</i> < 0.001). Patients with <i>RUNX1</i> mutation had worse OS than non-mutation patients in the early WHO group. <i>RUNX1</i> mutation and IPSS-M risk stratification mean OS and mean LFS were worse in low-risk patients than in non-mutated patients.</p><p><strong>Conclusion: </strong><i>RUNX1</i> gene mutation is an adverse prognostic factor in MDS patients, especially in the IPSS-M prognosis stratification group of low-risk, medium-low risk, medium-high risk and WHO classification of early patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Correlation of CD4⁺/CD8⁺Ratio in Peripheral Blood with Prognosis of Mantle Cell Lymphoma].","authors":"Yan-Ling Li, Xiao-Qi Qin, Lu-Yao Guo, Xiao-Xu Hou, Yao Chao, Yan-Ping Ma","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.024","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.04.024","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the correlation of peripheral blood T lymphocyte subsets with overall survival (OS) and clinical baseline characteristics in mantle cell lymphoma (MCL).</p><p><strong>Methods: </strong>The clinical data of 55 MCL patients who were newly diagnosed in the Department of Hematology, Second Hospital of Shanxi Medical University from January 2012 to July 2022 were analyzed retrospectively. The percentages of T lymphocyte subsets and CD4⁺/CD8⁺ ratio in peripheral blood were detected by flow cytometry, and their correlation with clinical characteristics of patients were analyzed. Kaplan-Meier method was used for survival analysis and survival curves were drawn. Log-rank test was used for univariate analysis, while Cox proportional hazards model was used for multivariate analysis.</p><p><strong>Results: </strong>The median follow-up was 40(1-68) months, and the median overall survival (OS) was 47 months. Among the 55 patients, 30(54.5%) patients had a decrease in peripheral blood CD4⁺T lymphocyte, while 17(30.9%) patients had a increase in peripheral blood CD8⁺T lymphocyte, and 20(36.4%) patients had a decrease in CD4⁺/CD8⁺ ratio. There were no significant correlations between CD4⁺/CD8⁺ ratio and sex, age, Ki-67, B symptoms, leukocytes, hemoglobin, lymphocytes, platelets, albumin, lactate dehydrogenase (LDH), β₂-microglobulin, splenomegaly, bone marrow invasion, primary site and MIPI score. Survival analysis showed that patients with CD4⁺T cell >23.3%, CD8⁺T cell ≤33.4% and CD4⁺/CD8⁺ ratio >0.6 had longer OS (<i>P</i> =0.020, <i>P</i> <0.001, <i>P</i> <0.001). Univariate analysis showed that Ki-67>30%, LDH>250 U/L, splenomegaly, bone marrow involvement, CD4⁺T cells ≤23.3%, CD8⁺ T cells >33.4%, CD4⁺/CD8⁺ ratio ≤0.6 were adverse prognostic factors affecting OS of MCL patients. Multivariate analysis showed that CD4⁺/CD8⁺ ratio ≤0.6 (<i>HR</i> =4.382, <i>P</i> =0.005) was an independent adverse prognostic factor for OS of MCL patients.</p><p><strong>Conclusions: </strong>Low CD4⁺/CD8⁺ ratio is associated with poor prognosis in MCL, and the CD4⁺/CD8⁺ ratio can be used as an important indicator to evaluate the prognosis risk in MCL patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Dobutamine Enhances the Targeted Inhibitory Effect of Quizartinib on <i>FLT3-ITD</i> Mutant Acute Myeloid Leukemia].","authors":"Yu-Ang Gao, Qian-Yu Zhang, Xin Li, Shen-Yu Wang, Ji-Hui Li, Yang Xue, Chang-Yan Li, Hong-Mei Ning","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.015","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2024.04.015","url":null,"abstract":"<p><strong>Objective: </strong>To observe the inhibitory effect of dobutamine on proliferation of <i>FLT3-ITD</i> mutated acute myeloid leukemia (AML) cells and explore the feasibility of dobutamine as a monotherapy or in combination with quizartinib for the treatment of this type of AML.</p><p><strong>Methods: </strong><i>FLT3-ITD</i> mutant cell lines MOLM13 and MV4-11 were cultured in vitro and divided into control group, dobutamine treatment group, quizartinib treatment group, and dobutamine combined with quizartinib treatment group. Cell viability, ROS levels, and apoptosis rate were detected by CCK-8, Flow cytometry, respectively, as well as the expression of YAP1 protein by Western blot.</p><p><strong>Results: </strong>Both dobutamine and quizartinib inhibited the proliferation of <i>FLT3-ITD</i> mutant AML cell lines. Compared with the control group, the dobutamine group exhibited a significant increase in ROS levels (<i>P</i> < 0.01), an increase in apoptosis rates (<i>P</i> < 0.05), and a decrease in YAP1 protein expression (<i>P</i> < 0.01), and decreased YAP1 expression (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Dobutamine as a monotherapy can inhibit theproliferation of <i>FLT3-ITD</i> mutated AML cells, inducing apoptosis. Additionally, the combination of quizartinib enhances the targeted inhibitory effect on <i>FLT3-ITD</i> mutated AML. The mechanism may involve the inhibition of YAP1 protein expression in AML cells of this type, leading to an increase in ROS levels and exerting its anti-tumor effects.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Inhibition Effect of Resveratrol on Spontaneous Senescence of Human Bone Marrow Derived Mesenchymal Stem Cells].","authors":"Yue Yang, Jian-Xin He","doi":"10.19746/j.cnki.issn.1009-2137.2024.04.039","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.04.039","url":null,"abstract":"<p><strong>Objective: </strong>To explore whether Resveratrol (RSV) can inhibit the spontaneous senescence of human bone marrow-derived mesenchymal stem cells (MSC).</p><p><strong>Methods: </strong>MSC were serially cultured to passage 13 and passage 15 to establish model groups exhibiting spontaneous senescence, respectively. MSC at passage 13 and passage 15 were treated with 5 nmol/L RSV for 48 h to establish the RSV-treated groups. SA-β-Gal staining was used to detect cell senescence. MTT assay was used to detect cell proliferation. RT-PCR method was used to detect senescenceassociated telomerase activity. Western blot was used to detect the senescence-associated protein level of the phosphorylated-mTOR.</p><p><strong>Results: </strong>SA-β-Gal staining showed that the senescent cells of MSC in RSV-treated group was significantly less than those in the model group (RSV group compared with model group at passage 13, <i>P</i> < 0.05; RSV group compared with model group at passage 15, <i>P</i> < 0.01). The cell proliferation ability of MSC in RSV-treated group was significantly higher than those in model group, at 72 h in passage 13, there was significant difference between RSV-treated group and model group (<i>P</i> < 0.05). RT-PCR results showed that the <i>hTERT</i> mRNA expression of MSC in RSV-treated group was higher than that in model group, which was significantly different between RSV-treated group and model group at passage 13 (<i>P</i> < 0.05). Western blot results showed that the phosphorylated (Ser2448)-mTOR level of MSC in RSV-treated group was lower than that in model group, which was significantly different between RSV-treated group and model group at passage 13 (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>RSV can inhibit the spontaneous senescence of human MSC by mediating mTOR activity.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}