中国实验血液学杂志最新文献

{"title":"[Molecular Pathogenic Mechanism Study of Two Cases of Inherited Dysfibrinogenemia].","authors":"Min Wang, Tian-Ping Chen, Ao-Shuang Jiang, Cheng-Lin Zhu, Nan Wei, Li-Juan Zhu, Li-Jun Qu, Hong-Jun Liu","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.027","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.027","url":null,"abstract":"<p><strong>Objective: </strong>To analyze two families with inherited dysfibrinogenemia, and explore the molecular pathogenic mechanisms.</p><p><strong>Methods: </strong>The coagulation indexes of the probands and their family members were detected. The <i>FGA, FGB</i>, and <i>FGG</i> exons and their flanking sequences were amplified by PCR, and the mutation sites were identified by sequencing. SIFT, PolyPhen2, LRT, ReVe, MutationTaster, phyloP, and phastCons bioinformatics software were used to predict the functional impact of the mutation sites. Protein structure and amino acid conservation analysis of the variant were conducted using PyMOL and Clustal X software.</p><p><strong>Results: </strong>The thrombin time (TT) of the proband in family 1 was prolonged to 37.00 s, and Fg∶C decreased to 0.52 g/L. The TT of the proband in family 2 was 20.30 s, and Fg∶C was 1.00 g/L, which was lower than the normal range. Genetic analysis revealed that the proband in family 1 had a heterozygous mutation c.80T>C in <i>FGA</i>, resulting in the substitution of phenylalanine 27 with serine (Phe27Ser). The proband in family 2 had a heterozygous mutation c.1007T>A in <i>FGG</i>, resulting in the substitution of methionine 336 with lysine (Met336Lys). Bioinformatics software prediction analysis indicated that both mutations were deleterious variants. PyMOL mutation models revealed that the Aα chain mutation (Phe27Ser) in family 1 and γ chain mutation (Met336Lys) in family 2 resulted in alterations in spatial structure and reduced protein stability. Clustal X results showed that both Aα Phe27 and γMet336 were highly conserved across homologous species.</p><p><strong>Conclusion: </strong>Heterozygous mutations of <i>FGA</i> gene c.80T>C and <i>FGG</i> gene c.1007T>A are both pathogenic variants, causing inherited dysfibrinogenemia.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"187-192"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical Characteristics and Prognosis Analysis of Angioimmunoblastic T-Cell Lymphoma].","authors":"Hong-Bo Wang, Xiu-Juan Wang, Xin-Hong Guo","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.015","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.015","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical characteristics and prognostic factors of patients with angioimmunoblastic T-cell lymphoma (AITL).</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the clinical data of 42 AITL patients who were diagnosed and monitored in the Hematologic Disease Center of the First Affiliated Hospital of Xinjiang Medical University from 2011 to 2021. Kaplan-Meier method was used to calculate overall survival (OS) rate and progression-free survival (PFS) rate, while log-rank method was utilized for comparison.</p><p><strong>Results: </strong>The majority of 42 patients were elderly, with a median age at diagnosis of 66(22-80) years old, and 25 cases were males. There were 38 patients in Ann Arbor stage III-IV, 23 patients with B symptoms, and 34 patients with intermediate-high to high risk of International Prognostic Index (IPI) score. The most common clinical manifestation was painless lymph node swelling (31 cases), which could be accompanied by multiple serous effusion (24 cases), fever (17 cases), rash (11 cases), and anemia (15 cases). There were 18 patients with positive Epstein-Barr virus (EBV), 28 patients with Ki-67 ≥40%, and 6 patients accompanied by EBV viremia. The median survival time of the 42 patients was 12(1-121) months, 3-year OS rate was 37.6%, and 3-year PFS rate was 26.1%. Univariate analysis indicated that patients with elevated interleukin (IL)-6 and multiple serous effusion exhibited lower survival rate.</p><p><strong>Conclusion: </strong>AITL is characterized by diverse clinical manifestations, high aggressiveness and poor prognosis. Conventional chemotherapy is not effective. Elevated IL-6 and multiple serous effusion are poor prognostic factors for AITL patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"108-113"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment and Application of an in Vitro Cellular Model of Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells with Serum Injury in aGVHD Mouse].","authors":"Run-Xiang Xu, Pei-Lin Li, Jia-Yi Tian, Jie Tang, Bo-Feng Yin, Fu-Hao Yu, Fei-Yan Wang, Xiao-Tong Li, Xiao-Yu Zhang, Wen-Rong Xia, Heng Zhu, Li Ding","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.038","DOIUrl":"10.19746/j.cnki.issn.1009-2137.2025.01.038","url":null,"abstract":"<p><strong>Objective: </strong>To establish an <i>in vitro</i> cell model simulating acute graft-versus-host disease (aGVHD) bone marrow microenvironment injury with the advantage of mouse serum of aGVHD model and explore the effect of serum of aGVHD mouse on the adipogenic differentiation ability of mesenchymal stem cells (MSCs).</p><p><strong>Methods: </strong>The 6-8-week-old C57BL/6N female mice and BALB/c female mice were used as the donor and recipient mice of the aGVHD model, respectively. Bone marrow transplantation (BMT) mouse model (<i>n</i>=20) was established by being injected with bone marrow cells (1×10⁷ per mouse) from donor mice within 4-6 hours after receiving a lethal dose (8.0 Gy, 72.76 cGy/min) of γ ray general irradiation. A mouse model of aGVHD (<i>n</i>=20) was established by infusing a total of 0.4 ml of a mixture of donor mouse-derived bone marrow cells (1×10⁷ per mouse) and spleen lymphocytes (2×10⁶ per mouse). The blood was removed from the eyeballs and the mouse serum was aspirated on the 7^th day after modeling. Bone marrow-derived MSCs were isolated from 1-week-old C57BL/6N male mice and incubated with 2%, 5% and 10% BMT mouse serum and aGVHD mouse serum in the medium, respectively. The effect of serum in the two groups on the <i>in vitro</i> adipogenic differentiation ability of mouse MSCs was detected by Oil Red O staining. The expression levels of related proteins PPARγ and CEBPα were detected by Western blot. The expression differences of key adipogenic transcription factors including <i>PPARγ, CEBPα, FABP4</i> and <i>LPL</i> were determined by real-time quantitative PCR (RT-qPCR).</p><p><strong>Results: </strong>An <i>in vitro</i> cell model simulating the damage of bone marrow microenvironment in mice with aGVHD was successfully established. Oil Red O staining showed that the number of orange-red fatty droplets was significantly reduced and the adipogenic differentiation ability of MSC was impaired at aGVHD serum concentration of 10% compared with BMT serum. Western blot experiments showed that adipogenesis-related proteins PPARγ and CEBPα expressed in MSCs were down-regulated. Further RT-qPCR assay showed that the production of <i>PPARγ, CEBPα, FABP4</i> and <i>LPL</i>, the key transcription factors for adipogenic differentiation of MSC, were significantly reduced.</p><p><strong>Conclusion: </strong>The adipogenic differentiation capacity of MSCs is inhibited by aGVHD mouse serum.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"255-261"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect and Safety of a New Conditioning Regimen with Chidamide and BEAM for Autologous Hematopoietic Stem Cell Transplantation in Lymphoma].","authors":"Sen Li, Jin-Jie Gao, Yan Li, Fei Dong, Qi-Hui Li, Wei Zhao, Wei Wan, Ping Yang, Ji-Jun Wang, Hong-Mei Jing","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.017","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.017","url":null,"abstract":"<p><strong>Objective: </strong>To assess the efficacy and safety of a new conditioning regimen with chidamide and BEAM for autologous hematopoietic stem cell transplantation (AHSCT) in patients with lymphoma.</p><p><strong>Methods: </strong>Medical records and further follow-up data from 85 patients with lymphoma from May 2015 to September 2020 in our hospital were retrospectively collected and analyzed.</p><p><strong>Results: </strong>Among 85 patients, 52 cases accepted BEAM regimen and 33 cases accepted CBEAM followed by AHSCT. In CBEAM group, 18 patients (54.5%) received AHSCT as salvage therapy, while only 26.9% (14 cases) for salvage in BEAM group ( <i>P</i> < 0.01). CBEAM conditioning resulted in shorter neutrophil engraftment of 2 days, while no significant difference was found in platelet engraftment. Although the incidence of liver impairment was higher in CBEAM group (12.1%), the grade of impairment was only Ⅰ to Ⅱ. The two conditioning regimens both achieved good complete remission rate of over 90%, and no transplant-related death occurred. The median follow-up time in the CBEAM group was 18(12, 22) months, and 39(20, 59) months in the BEAM group. There were no significantly differences in 2-year progression-free survival (PFS) and overall survival (OS) rate between the two groups (<i>P</i> >0.05). In patients with refractory or relapsed non-Hodgkin lymphoma, the 2-year PFS rate after transplantation in BEAM group and CBEAM group was 74.1% and 92.9%, respectively (<i>P</i> >0.05), indicating that chidamide may have certain advantages in prolonging PFS.</p><p><strong>Conclusion: </strong>CBEAM conditioning regimen has a good efficacy and safety in lymphoma patients before AHSCT, especially in refractory and relapsed non-Hodgkin lymphoma patients, suggesting that it could serve as an alternative conditioning regimen prior to AHSCT for lymphoma.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"121-126"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expression and Clinical Significance of Co-inhibitory Molecules TIGIT/CD155 and PD-1 in Chronic Lymphocytic Leukemia].","authors":"Rui Zhang, Shuang Chen, Ting-Ting Luo, Jian-Hua Qu","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.008","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.008","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression of co-inhibitory molecules TIGIT/CD155 and PD-1 on CD4⁺T cells and Treg cells in peripheral blood of patients with chronic lymphocytic leukemia (CLL) and analyze their clinical significance.</p><p><strong>Methods: </strong>The expression of PD-1 and TIGIT on CD4⁺T cells and Treg cells was detected by flow cytometry in 40 CLL patients and 20 healthy controls. Additionally, the expression of CD155 on peripheral blood B cells and DC cells of the enrolled subjects was detected.</p><p><strong>Results: </strong>The proportions of PD-1⁺TIGIT⁺CD4⁺T cells, PD-1⁺TIGIT⁺Treg cells and CD155⁺DC cells in peripheral blood of CLL patients were significantly higher than those of healthy controls ( <i>P</i> < 0.05). The proportions of PD-1⁺TIGIT⁺CD4⁺T cells and PD-1⁺TIGIT⁺Treg cells in CLL patients were significantly higher than those of PD-1⁺TIGIT^-CD4⁺T cells and PD-1⁺TIGIT^-Treg cells, respectively ( <i>P</i> < 0.05). Both PD-1⁺TIGIT⁺CD4⁺T cells and PD-1⁺TIGIT⁺Treg cells were positively correlated with the level of CD155⁺DC cells (<i>r</i> =0.742, <i>r</i> =0.766). With the progression of Binet stage, the proportions of PD-1⁺TIGIT⁺CD4⁺T cells, PD-1⁺TIGIT⁺Treg cells, and CD155⁺DC cells gradually increased ( <i>P</i> < 0.05), and the aforementioned three types cells were all increased in patients with CD38≥30%, <i>IGVH</i> unmutated, or poor prognosis due to chromosomal abnormalities ( <i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Co-inhibitory molecules PD-1 and TIGIT may be involved in immunodepletion in patients with advanced CLL, which has clinical prognostic value. Dual inhibitor molecular targeted therapy provides a new direction for the individualized treatment of CLL.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"54-61"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Ginsenoside-Rg5 Synergizes with Imatinib to Enhances the Anti-Chronic Myeloid Leukemia K562 Cell Activity through PI3K/AKT/mTOR Pathway].","authors":"Di Jin, Chang-Qing Gui, Qian-Qian Ye, Guo-Fang Deng, Chang-Ling Zhu, Li Xu","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.001","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the synergistic effect and its mechanism of ginsenoside-Rg5 in combination with imatinib in inhibiting proliferation of chronic myeloid leukemia K562 cells.</p><p><strong>Methods: </strong>K562 cells were treated with ginsenoside-Rg5 and imatinib. Cell survival was detected by CCK-8 assay, and IC₅₀ were calculated separately for each drug. Based on the value of IC₅₀ of ginsenoside-Rg5 and imatinib, an appropriate concentration gradient was selected for the combination. The synergistic effect of the two drug was analyzed using the online software synergy finder. The effects of single or combination therapy on apoptosis rate and the cell cycle distribution of K562 cells were analyzed by flow cytometry. Western blot was used to detect the expression of PI3K/AKT/mTOR signaling pathway related proteins and apoptosis related proteins in K562 cells after single or combination therapy.</p><p><strong>Results: </strong>Ginsenoside-Rg5 and imatinib were able to inhibit the proliferative activity of K562 cells in a dosedependent manner(<i>r</i> =-0.991， <i>r</i> =-0.942). The synergy score ZIP >10 was measured by Synergy Finder online software, indicating that ginsenoside-Rg5 and imatinib act synergistically on K562 cells. The apoptotic rates of K562 cells after single treatments with ginsenoside-Rg5 and imatinib were 11.96% and 8.13%, respectively, while the rate increased to 21.35% with the combination of two drugs, the apoptosis rate in the combination group was higher than that in the single-drug group ( <i>P</i> <0.05). The proportion of K562 cells in the G₀/G₁ phase was significantly increased with the combined treatment of two drugs( <i>P</i> <0.05). The protein expression levels of p-PI3K, p-AKT, p-mTOR in K562 cells treated with the combination were significantly decreased, with noticeable downregulation of BCL-2 and upregulation of BAX, leading to a decreased Bcl-2/BAX ratio, while no significant changes were observed in the non-phosphorylated forms of PI3K, AKT, and mTOR proteins.</p><p><strong>Conclusion: </strong>The combination of ginsenoside-Rg5 and imatinib can inhibit the proliferation of CML cells and induce apoptosis, and the mechanism may act through PI3K/AKT/mTOR signaling pathways.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research Progress on Tyrosine Kinase Inhibitors Discontinuation in Patients with Chronic Myeloid Leukemia --Review].","authors":"Jun-Xia He, Xi-Yuan Jiang, Ya-Ming Xi","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.046","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.046","url":null,"abstract":"<p><p>Tyrosine kinase inhibitors (TKIs) have significantly improved the prognosis of patients with chronic myeloid leukemia (CML), and achieving treatment-free remission (TFR) has become a new goal for these patients. Various methods of discontinuing medication are continuously being explored, with successful cessation linked to factors such as the duration of TKI treatment, the sustainability of deep molecular response (DMR), transcript type, and immunological factors. Early switching of TKI, combining other therapies, and targeting leukemia stem cells may help increase the TFR rate. This article reviews the latest research developments on the exploration of TKI cessation, factors affecting TFR, selection of patients for discontinuation, strategies to enhance TFR, and monitoring after cessation, expecting to provide reference and guidance for achieving TFR in CML patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"300-305"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A Retrospective Analysis of Irregular Erythrocyte Antibodies in the Blood Transfusion Department of People's Hospital of Xinjiang Uygur Autonomous Region from 2011 to 2022].","authors":"Ru-Bin Wang, Hui-Jun Li, Fei Li, Wei Chen","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.031","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.031","url":null,"abstract":"<p><strong>Objective: </strong>The distribution of irregular erythrocyte antibodies in the blood transfusion department of the People's Hospital of Xinjiang Uygur Autonomous Region from 2011 to 2022 and the relationship between irregular erythrocyte antibodies and ethnicity, gender, pregnancy history, blood transfusion history were retrospectively analyzed.</p><p><strong>Methods: </strong>The irregular antibody screening data of patients who were proposed to receive blood transfusions in the clinical blood transfusion safety and blood management software of our hospital from 2011 to 2022 were collected for a retrospective study, and the distribution of irregular erythrocyte antibodies from 2011 to 2022 was analyzed. The relationship between ethnicity, gender, pregnancy history, blood transfusion history and the detection rate of irregular erythrocyte antibodies was further analyzed.</p><p><strong>Results: </strong>From 2011 to 2022, the positive detection rate of irregular erythrocyte antibodies in 329 270 samples was 0.77%. Rh blood group (43.72%), Lewis blood group (9.90%) and MNS blood group (6.44%) accounted for the highest proportion of irregular erythrocyte antibody positive samples. In Rh blood group, the proportion of anti-D and anti-E in positive samples was the highest, with 19.09% and 16.06%, respectively. In MNS blood group, the proportion of anti-M in positive samples was the highest (5.46%). In Lewis blood group, the proportion of anti-Le^a in positive samples was the highest (8.80%). Compared with other ethnic groups, the detection rates of irregular erythrocyte antibodies were significantly higher in Han, Hui and Uyghur ethnic groups (<i>P</i> < 0.001). Irregular erythrocyte antibody positive samples in Rh blood group system were concentrated in Han and Uygur ethnic groups. Compared to males and patients without a history of blood transfusion and pregnancy, female patients and patients with a history of blood transfusion and pregnancy had significantly higher detection rates of irregulart erythrocyte antibodies (<i>P</i> < 0.01).</p><p><strong>Conclusion: </strong>The results of irregular antibody screening before blood transfusion showed that Rh blood group system antibodies were the main type of irregular antibodies, and the screening of various Rh blood group antigens should be strengthened. And the screening should be focused on female, patients with blood transfusion history and pregnancy history, as well as ethnic minority patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"211-216"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of Delayed Hemolytic Transfusion Reaction in Children with Repeated Blood Transfusion].","authors":"Li-Lan Gao, Meng-Xing Lyu, Shu-Xia Wang, Xiao-Hong Jin, Jian-Xiang Liu, Mei-Kun Hu, Ke-Xuan Qu","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.032","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.032","url":null,"abstract":"<p><strong>Objective: </strong>To summarize and analyze the characteristics of delayed hemolytic transfusion reaction in children, in order to provide a scientific basis for clinical prevention, and ensure the safety of children's blood transfusion.</p><p><strong>Methods: </strong>The basic situation, clinical symptoms and signs, diagnosis time and disappearance time of alloantibody of delayed hemolytic transfusion reaction in children were retrospectively analyzed. The serological test, routine blood test, biochemical detection and urine analysis results were compared pre- and post-transfusion.</p><p><strong>Results: </strong>Among 15 164 children with repeated blood transfusion, 23 cases occurred delayed hemolytic transfusion reactions, with an incidence rate of 0.15%, and mainly children with thalassemia and acute leukemia. 39.13% of delayed hemolytic reactions occurred in children with more than 20 times of blood transfusions. Anemia was the main clinical symptom in 86.96% of children. 4.35% of children had hypotension and dyspnea. Serological test results showed that the positive rate of direct antiglobulin test was 91.30%, and that of erythrocyte homologous antibody test was 100%. Erythrocyte alloantibodies were common in Rh and Kidd blood group systems, accounting for 73.91% and 13.04%, respectively. Laboratory test results showed that hemoglobin, reticulocyte, spherocyte, total bilirubin, indirect bilirubin, lactate dehydrogenase, serum ferritin and urine color were significantly different after transfusion compared with those before transfusion (all <i>P</i> <0.05). The average diagnosis time of delayed hemolytic transfusion reactions was 18.56 days, and the average disappearance time of erythrocyte alloantibodies was 118.43 days.</p><p><strong>Conclusion: </strong>The incidence of delayed hemolytic transfusion reaction is high in children with repeated blood transfusion, and the disappearance time of erythrocyte homologous antibody is long. Blood matched ABO, Rh and Kidd blood group antigens should be transfused prophylactically. Once diagnosed, erythrocyte alloantibody corresponding to antigen-negative blood should be used throughout the whole process.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"217-223"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prognostic Value of Plasma Fibrinogen Levels in Patients with Diffuse Large B-Cell Lymphoma].","authors":"Bing Zhang, Lin Lin, Jian-Min Ji, Yu Wu, Qun Shen","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.016","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.016","url":null,"abstract":"<p><strong>Objective: </strong>To assess the prognostic significance of plasma fibrinogen（FIB） levels in patients of diffuse large B-cell lymphoma(DLBCL).</p><p><strong>Methods: </strong>We retrospectively analyzed 203 newly diagnosed with DLBCL patients who met the study requirements from November 2016 to May 2024. Based on the receiver operating characteristic (ROC) curve analysis of plasma FIB levels during diagnosis, the critical value of FIB was determined, and patients were divided into high FIB and low FIB groups. The clinical characteristics and relevant laboratory indicators of two groups were compared. The impact of plasma FIB levels on overall survival (OS) were evaluated using Kaplan-Meier curves as well as univariate and multivariate Cox regression analysis. The differences in FIB and other laboratory indicators under different disease states were compared.</p><p><strong>Results: </strong>According to the ROC curve, the optimal cut-off value of FIB was 3.49 g/L. Compared with the high FIB group (>3.49 g/L), the low FIB group (≤3.49 g/L) had a significant decrease in neutrophil count (ANC) (<i>P</i> =0.001) and platelet count (PLT) (<i>P</i> =0.027), and a significant increase in prealbumin (PA) (<i>P</i> =0.001). A high FIB level was associated with decreased OS (<i>P</i> =0.005). Univariate analysis results showed that FIB had an impact on survival of patients(<i>HR</i>＝2.031，95%<i>CI</i> : 1.221-3.375, <i>P</i> =0.006). Multivariate analysis showed that higher FIB level was an independent adverse prognostic factor affecting patients survival （<i>HR</i>＝2.684， 95%<i>CI</i> :1.478-4.875, <i>P</i> =0.001）. Compared with patients with newly diagnosed or recurrent DLBCL, patients with complete remission showed a significant decrease in FIB (<i>P</i> _ND < 0.001, <i>P</i> _R=0.001) and ANC (<i>P</i> _ND < 0.001, <i>P</i> _R=0.021), as well as an increase in albumin (ALB) (<i>P</i> _ND < 0.001, <i>P</i> _R=0.018) and PA (<i>P</i> _ND < 0.001, <i>P</i> _R < 0.001).</p><p><strong>Conclusion: </strong>Elevated FIB is a poor prognostic factor for DLBCL patients. The plasma FIB level is correlated with laboratory indicators such as ANC, PLT, PA, and disease status in DLBCL patients. Dynamic monitoring can assist in the early detection of changes in the condition.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"114-120"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}