中国实验血液学杂志最新文献

{"title":"[Expression and Correlation of Serum MCV, MPV and <i>WT-1</i> in Elderly Patients with MDS].","authors":"Huan-Ying Li, Jun-Xia Wang, Fei Guo, Ai-Hua Cheng","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.024","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.024","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression levels and combined detection efficiency of serum mean corpuscular volume (MCV), mean platelet volume (MPV), and tumor gene ( <i>WT-1</i>) in elderly patients with myelodysplastic syndrome (MDS).</p><p><strong>Methods: </strong>One hundred elderly MDS patients admitted to our hospital from January 2020 to January 2021 were selected as observation group, and eighty healthy subjects during the same period were selected as control group. The levels of MCV, MPV and <i>WT-1</i> were detected, and receiver operating characteristic (ROC) curve was drawn to analyze the value of combined detection of the three indicators in the prediction of MDS. The expression and correlation of MCV, MPV and <i>WT-1</i> in elderly patients with MDS were analyzed and evaluated.</p><p><strong>Results: </strong>The levels of MCV, MPV, and <i>WT-1</i> in the observation group were higher than those in the control group (all <i>P</i> <0.001). Pearson correlation analysis showed that MCV was positively correlated with MPV and <i>WT-1</i> (<i>r</i> =0.724, 0.733), while MPV was positively correlated with <i>WT-1</i> (<i>r</i> =0.731). MCV, MPV, and <i>WT-1</i> were independent influencing factors for elderly MDS (all <i>P</i> <0.05). The combined detection of the three indicators had the largest area under the curve (AUC) (0.873, 95%<i>CI</i> : 0.776-0.893) in the diagnosis of elderly MDS, with a sensitivity of 95.00%, a specificity of 90.00%, and Youden index of 0.850. The diagnostic value was significantly higher than that of a single indicator (both <i>P</i> <0.05). The levels of MCV, MPV, and <i>WT-1</i> in severe patients were significantly higher than those in mild patients (all <i>P</i> <0.01). Logistic regression analysis showed that high expression of MCV, MPV, and <i>WT-1</i> were influencing factors of severe elderly MDS. The ROC curve analysis showed that the combined diagnosis of MCV, MPV and <i>WT-1</i> had the largest AUC for predicting severe MDS in elderly patients (0.897, 95%<i>CI</i> : 0.709-0.926), and the sensitivity and specificity were 93.18% and 91.07%, respectively (<i>P</i> <0.05).</p><p><strong>Conclusion: </strong>MCV, MPC, and <i>WT-1</i> are highly expressed in elderly patients with severe MDS. These three indicators can reflect the bone marrow hematopoietic function status of the subjects. However, compared with single indicator detection, the combined detection of the three indicators is more effective in diagnosis. It has certain advantages in elderly MDS and disease staging, and its promotion and application value is higher.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"475-480"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prognostic Value of <i>CDKN2A</i> Copy Number Deletion in Patients with Diffuse Large B-Cell Lymphoma].","authors":"Wei-Yuan Ma, Le-Tian Shao, Wen-Xin Tian, Sha Liu, Yan Li","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.011","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.011","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the relationship between <i>CDKN2A</i> copy number deletion and clinical features of patients with diffuse large B-cell lymphoma (DLBCL) and its prognostic value.</p><p><strong>Methods: </strong>155 newly diagnosed DLBCL patients with complete clinical data in the Department of Hematology of People's Hospital of Xinjiang Uygur Autonomous Region from March 2009 to March 2022 were included, formalin-fixed paraffin-embedded tumor tissues were obtained and DNA was extracted from them, and next-generation sequencing technology was applied to target sequencing including 475 lymphoma-related genes, the relationship between <i>CDKN2A</i> copy number deletion and clinical features, high-frequency mutated genes and overall survival (OS) of DLBCL patients were analyzed.</p><p><strong>Results: </strong><i>CDKN2A</i> copy number deletion was present in 12.9% (20/155) of 155 DLBCL patients, grouped according to the presence or absence of copy number deletion of <i>CDKN2A</i>, and a higher proportion of patients with IPI≥3 were found in the <i>CDKN2A</i> copy number deletion group compared to the group with no <i>CDKN2A</i> copy number deletion (80% <i>vs</i> 51.5%, <i>P</i> =0.015) and were more likely to have bulky disease (20% <i>vs</i> 5.2%, <i>P</i> =0.037). Survival analysis showed that the 5-year OS of patients in the <i>CDKN2A</i> copy number deletion group was significantly lower than that of the non-deletion group (51.3% <i>vs</i> 69.2%, <i>P</i> =0.047). Multivariate Cox analysis showed that IPI score≥3 (<i>P</i> =0.007), <i>TP53</i> mutation (<i>P</i> =0.009), and <i>CDKN2A</i> copy number deletion (<i>P</i> =0.04) were independent risk factors affecting the OS of DLBCL patients.</p><p><strong>Conclusion: </strong><i>CDKN2A</i> copy number deletion is an independent risk factor for OS in DLBCL, and accurate identification of <i>CDKN2A</i> copy number deletion can predict the prognosis of DLBCL patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"379-386"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Correlation between miR451a and Occult Hepatitis B Virus Infection in Voluntary Blood Donors].","authors":"Qiang Liu, Wei Yu, Fang Wang","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.035","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.035","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the relationship between miR451a and occult hepatitis B virus infection (OBI) in voluntary blood donors, and to provide ideas for the identification of OBI.</p><p><strong>Methods: </strong>A total of 125 003 blood samples were collected from voluntary blood donors in our center from January 2022 to June 2023, and OBI infection was detected by blood screening. At the same time, 40 HBsAg double reagent reactive samples (S/CO>3.0) were selected as the positive control group, and 40 healthy blood donors were selected as the negative control group (normal group). The plasma miR451a level was detected, and the serum indexes of total bilirubin (TBil), alanine aminotransferase (ALT), aspartate aminotransferase (AST). The relationship between miR451a and OBI were analyzed.</p><p><strong>Results: </strong>54 out of 125 003 blood samples were diagnosed as OBI, and the OBI infection rate was 0.043% (54/125 003). Compared with the normal group, the relative expression of plasma miR451a in the OBI group and the positive control group was down-regulated (<i>P</i> < 0.05), but there was no significant difference in the relative expression of plasma miR451a between the OBI group and the positive control group (<i>P</i> >0.05). The HBV DNA load, TBil, ALT and AST levels in the positive control group were higher than those in the OBI group and the normal group (<i>P</i> < 0.05). There was no significant difference in plasma TBil, ALT and AST levels between OBI group and normal group (<i>P</i> >0.05). Logistic regression analysis and receiver operating curve (ROC) showed that plasma miR451a could distinguish OBI group from healthy group, and the area under the curve (AUC) was 0.904 (95%<i>CI</i> : 0.829-0.978). However, plasma miR451a was difficult to distinguish between OBI and HBsAg responders.</p><p><strong>Conclusion: </strong>Plasma miR451a can be used as a potential biomarker for HBV infection, and can be used to identify OBI in HBsAg non-reactive blood donors.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"546-551"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Latest Research Progress of T Cell Exhaustion in Hematological Malignancy --Review].","authors":"Xia-Xin Liu, Zi-Zhen Xu, Jun-Min Li","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.045","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.045","url":null,"abstract":"<p><p>T cell exhaustion plays an immunosuppressive role in malignant tumors. Continuous tumor antigen stimulation, the presence of suppressive immune cells and cytokines in the tumor microenvironment, the up regulation of inhibitory receptor expression on the surface of T cells, changes in T cell related transcription factors, and metabolites in the tumor microenvironment may lead to T cell exhaustion. Reversing the exhaustion of T cells in tumor patients is a promising strategy for tumor immunotherapy. This article will review the latest research progress on T cell exhaustion status, pathogenesis, reversal methods, and clinical applications in hematological tumors.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"606-611"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on Differential DNA Methylation Profiles of Patients with High-Altitude Polycythemia].","authors":"Jun-Hua Ji, Min Yang, Yan Jiang, Ting-Xian Yang, Xiao-Jing Ma, Qi-Chao Yin, Hong-Wei Yin, Lin-Hua Ji","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.041","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.041","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the whole-genome differential methylation profile of patients with high-altitude polycythemia (HAPC).</p><p><strong>Methods: </strong>In this study, a total of 20 adult male patients with HAPC were included, including 10 Tibetan and 10 Han patients. The control group consisted of 20 healthy adult males, including 10 Tibetan and 10 Han patients. Peripheral blood was collected from each group for DNA extraction and quality inspection, and DNA libraries were constructed. The differential methylation regions (DMRs) between groups were detected using reduced representation bisulfite sequencing, with enriched regions compared to those of the control group. The differential enrichment regions were selected, and the intersection of the enriched regions was associated with genes. The methylation enrichment regions that differed significantly between groups were filtered based on the number of enriched samples in the enriched regions between the groups. GO, KEGG functional, and pathway analysis were performed on the differentially associated gene sets to reveal significant differences between the patients and control groups at the functional and pathway levels.</p><p><strong>Results: </strong>In comparison with the control group, 17 152 sites with more than 25% difference and 15 558 sites with less than -25% difference were identified in Tibetan patients. The top 5 genes with the largest methylation differences between the two groups were <i>MCCC2, RP3-399L15.3, ZNF621, RP11-394A14.2</i> and <i>SLC39A10.</i> The top significantly different pathways annotated in the differentially expressed genes pathway was serotonergic synapse. In comparison with the control group, 2 687 CpG sites with a greater than 25% difference and 2 602 CpG sites with a less than -25% difference were identified in Han patients. The top 5 genes with the largest methylation differences between the two groups were <i>NAA25, CORO2B, PDC, ZNF853</i>, and <i>MLLT10</i>. The top significantly different pathways annotated in the differentially expressed genes pathway were glutamatergic synapse, retrograde endocannabinoid signaling, Rap1 signaling pathway and cholinergic synapse. In comparison with the control group, 3 895 CpG sites with a greater than 25% difference and 3 969 CpG sites with a less than -25% difference were identified in HAPC patients. The maximum methylation difference between the two groups could reach 78.1%, while the minimum was -42.6%. The top 5 genes with the largest methylation differences between the two groups were <i>MCCC2, ARSJ, CTNNA3, SLC39A10</i>, and <i>SWAP70</i>. The top significantly different pathways annotated in the differentially expressed genes pathway was signaling pathways regulating pluripotency of stem cells.</p><p><strong>Conclusion: </strong>The occurrence of HAPC may be related to abnormal changes in DNA methylation, and methylation sites may be helpful for the early diagnosis of HAPC.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"580-586"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Genotyping and Transfusion Strategy for Pregnant Patients with ABO Blood Typing Difficulties].","authors":"Chen-Chen Feng, Qing Chen, Xiao Wei, Li-Li Shi, Ruo-Yang Zhang, Fang Zhao, Jian-Yu Xiao","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.034","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.034","url":null,"abstract":"<p><strong>Objective: </strong>To identify the blood type of specimens from pregnant patients with difficult-to-type ABO status, and to guide clinical safe blood transfusion.</p><p><strong>Methods: </strong>The specimens from 36 pregnant patients with suspicious ABO blood group were collected. These specimens were submitted by clinical institutions from various regions to our center's genetic testing platform from January 2021 to December 2022. The blood group phenotypes and genotypes of these specimens were identified by serological method and genetic sequencing.</p><p><strong>Results: </strong>A total of 20 ABO subtypes were detected in the 36 samples, including 10 cases of <i>BA/O</i>, 3 cases of <i>cisAB/O</i>, 2 cases of <i>A/Bw</i>, 1 case of <i>A2/B</i>, 1 case of <i>Aw/B</i>, 1 case of <i>BA/B</i>, 1 case of <i>BA/A</i>, and 1 case of <i>Bw/O</i>. Additionally, 4 cases were identified as para-Bombay blood type, and no specific variations associated with abnormal phenotypes were found in the remaining 12 cases.</p><p><strong>Conclusion: </strong>ABO subtypes interfere with ABO blood group identification in pregnant patients, and pregnancy status also affects blood group phenotype. Accurate determination of blood group genotype by genetic sequencing technology can guide clinical blood transfusion for pregnant patients, and ensure maternal and infant safety.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"538-545"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical Analysis of Extranodal NK/T-Cell Lymphoma, Nasal Type with Skin Lesions as Initial Symptom].","authors":"Ping Cheng, Yi Li, Xia Mao, Qiu-Xiang Wang, Lan-Lan Wang, Jun Guan, Ying Zhou, Hui Cheng","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.015","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.015","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical features, treatment and prognosis of extranodal NK/T-cell lymphoma, nasal type (ENKTL) with skin lesions as initial symptom.</p><p><strong>Methods: </strong>The clinical data of 11 ENKTL patients with skin lesions as initial symptom were retrospectively analyzed from August 2016 to January 2023 in Wuhan First Hospital and Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology.</p><p><strong>Results: </strong>Among the 11 patients, there were 6 males and 5 females, with a median age of 50(32-80) years. All patients had different forms of skin lesions as initial clinical symptom, including rash, ulcerative mass, painful skin nodules, infiltrating macula, etc. Most of the skin lesions were involved in the limbs and trunk but also appeared in the lower limbs alone. Five patients had hemophagocytic lymphohistiocytosis (HLH) at initial diagnosis, and 8 patients had B symptoms. All patients were diagnosed with advanced clinical staging (Lugano staging IV), and classified as high risk (PINK-E score ≥3). Immunohistochemical examination revealed that the positive rates of CD56 and EBER were both 100%, and the median Ki-67 index was 75%(50%-80%). Plasma EBV-DNA tests were all positive (≥5×10² copies/ml). Most of the induction chemotherapy regimens were combination chemotherapy (MESA, p-Gemox, SMILE) containing pegaspargase or L-asparaginase, or combined with PD-1 monoclonal immunotherapy, or HLH regimens (HLH-04 regimen, L-DEP). The median follow-up time and overall survival (OS) time were both 4.5(0.5-27) months. During the follow-up period, all 8 patients who did not receive autologous hematopoietic stem cell transplantation (ASCT) died, most of whom died of rapid disease progression. Three patients received ASCT, one died of central nervous system recurrence after transplantation, and two survived. The OS of three patients who underwent ASCT was 21, 27, and 19 months, and PFS was 11, 20, and 13 months, respectively. The plasma EBV-DNA copy number was monitored irregularly after transplantation, and the load of EBV was consistent with the changes of the disease.</p><p><strong>Conclusions: </strong>Early clinical symptoms of ENKTL patients with skin lesions as initial symptom are more atypical, and early diagnosis is particularly difficult. The disease progresses rapidly and the prognosis is poor. There is still no uniform standard for the best treatment strategy. The survival of patients can be significantly prolonged by applying ASCT as soon as possible after complete remission obtained by high-dose induction chemotherapy.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"416-422"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Significance of HALP Score as a Prognostic Indicator for Newly Diagnosed Multiple Myeloma].","authors":"Kuan-Shun Zhang, Dian-Liang Lyu, Lin Shi","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.019","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.019","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the significance of HALP score as a prognostic indicator for newly diagnosed multiple myeloma (MM).</p><p><strong>Methods: </strong>Clinical data of 62 newly diagnosed MM patients in our hospital from January 2020 to December 2022 were collected and retrospectively analyzed. The difference in age, sex, DS stage, R-ISS stage, M protein type, serum creatinine (Scr), β₂-microglobulin (β₂-MG), blood calcium, lactate dehydrogenase (LDH), hemoglobin (Hb), albumin (ALB), platelet count (PLT), and absolute lymphocyte count (ALC) between patients with low and high HALP scores were analyzed. The prognostic value of the above indexes in newly diagnosed MM patients was analyzed by univariate and multivariate analysis.</p><p><strong>Results: </strong>The optimal cut-off value of HALP score was 41 determined by X-tile software. Based on this, 62 patients were divided into a high HALP group (HALP>41, <i>n</i>=25) and a low HALP group (HALP≤41, <i>n</i>=37). The proportion of patients with Hb≥100 g/L in the high HALP group was significantly higher than that in the low HALP group (<i>P</i> <0.05). The median overall survival (OS) time of patients in the high HALP group and low HALP group were 29 (9-39) months and 20 (4-29) months, respectively, with statistically significant difference between the two groups (<i>P</i> <0.01). Univariate analysis showed that R-ISS stage, ALB, Hb, ALC and HALP were closely related to the prognosis of patients (<i>P</i> <0.05). COX regression multivariate analysis showed that R-ISS stage Ⅲ (<i>HR</i>=4.443, 95%<i>CI</i> : 1.480-13.343，<i>P</i> =0.008) and HALP≤41(<i>HR</i>=8.823, 95%<i>CI</i> : 1.858-41.910，<i>P</i> =0.006) were independent risk factors for shortened OS in newly diagnosed MM patients. The median OS of patients with high HALP at R-ISS stage Ⅲ was significantly higher than that of patients with low HALP at the same stage, and the difference was statistically significant (<i>P</i> <0.05).</p><p><strong>Conclusion: </strong>HALP score can be used as a prognostic indicator for newly diagnosed MM patients.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"442-446"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Value of Pathogenic Detection by Next-Generation Sequencing in Bronchoalveolar Lavage Fluid on Children with Hematological Malignancies].","authors":"Bin Wu, Jie Wang, Lan-Nan Zhang, Wei Tang, Kai-Lan Chen","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.039","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.039","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the application value of bronchoalveolar lavage fluid (BALF) metagenomic next-generation sequencing (mNGS) in etiological diagnosis of children with hematological malignancies complicated with pneumonia.</p><p><strong>Methods: </strong>We retrospectively analyzed the clinical data of children with hematological malignancies who underwent BALF mNGS pathogenic detection due to pneumonia. All patients underwent mNGS detection of bronchoalveolar lavage fluid as well as traditional methods(including sputum culture, bronchoalveolar lavage fluid culture, blood culture, serological detection of pathogens, etc.). By analyzing the results of mNGS and traditional methods, we compared key indicators such as the positive rate, etiological distribution.</p><p><strong>Results: </strong>A total of 26 children with hematological malignancies enrolled in the study, including 12 males and 14 females, with a median age of 4.9 (1.8-14.9) years, underwent bronchoalveolar lavage (BAL) 35 times. A total of 17 pathogenic microorganisms were detected in BALF mNGS, including 9 cases of bacterial infection, 10 cases of viral infection, 3 cases of fungal infection, 2 cases of mycoplasma infection and 8 cases of mixed infection, and the most commonly detected bacteria, viruses and fungi were streptococcus pneumoniae, cytomegalovirus and pneumocystis jirovecii, respectively. The positive rate of mNGS detection (91.43%) was significantly higher than that of traditional methods detection (20%, <i>P</i> <0.001). A total of 25 cases were adjusted according to BALF mNGS results.</p><p><strong>Conclusion: </strong>The application of BALF mNGS technology can improve the detection rate of the pathogens in children with hematological malignancies complicated with pneumonia, initially revealed the pathogen spectrum of pulmonary infection in this group, and effectively guide clinical medication, improve treatment outcomes.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"569-574"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of <i>TBL1XR1</i> Mutation on Cell Biological Characteristics of Diffuse Large B-Cell Lymphoma].","authors":"Hong-Ming Fan, Le-Min Hong, Chun-Qun Huang, Jin-Feng Lu, Hong-Hui Xu, Jie Chen, Hong-Ming Huang, Xin-Feng Wang, Dan Guo","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.016","DOIUrl":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.016","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of <i>TBL1XR1</i> mutation on cell biological characteristics of diffuse large B-cell lymphoma (DLBCL).</p><p><strong>Methods: </strong>The <i>TBL1XR1</i> overexpression vector was constructed and DNA sequencing was performed to determine the mutation status. The effect of <i>TBL1XR1</i> mutation on apoptosis of DLBCL cell line was detected by flow cytometry and TUNEL fluorescence assay; CCK-8 assay was used to detect the effect of <i>TBL1XR1</i> mutation on cell proliferation; Transwell assay was used to detect the effect of <i>TBL1XR1</i> mutation on cell migration and invasion; Western blot was used to detect the effect of <i>TBL1XR1</i> mutation on the expression level of epithelial-mesenchymal transition (EMT) related proteins.</p><p><strong>Results: </strong>The <i>TBL1XR1</i> overexpression plasmid was successfully constructed. The <i>in vitro</i> experimental results showed that <i>TBL1XR1</i> mutation had no significant effect on apoptosis of DLBCL cells. Compared with the control group, <i>TBL1XR1</i> mutation enhanced cell proliferation, migration and invasion of DLBCL cells. <i>TBL1XR1</i> gene mutation significantly increased the expression of N-cadherin protein, while the expression of E-cadherin protein decreased.</p><p><strong>Conclusion: </strong><i>TBL1XR1</i> mutation plays a role in promoting tumor cell proliferation, migration and invasion in DLBCL. <i>TBL1XR1</i> could be considered as a potential target for DLBCL therapy in future research.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"423-430"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}