Sudebno-Meditsinskaya Ekspertisa最新文献_第6页

[Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay].

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246706121

A A Gusarov, V L Sidorov, O D Yagmurov, N E Surikova

{"title":"[Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay].","authors":"A A Gusarov, V L Sidorov, O D Yagmurov, N E Surikova","doi":"10.17116/sudmed20246706121","DOIUrl":"https://doi.org/10.17116/sudmed20246706121","url":null,"abstract":"Objective: Improving the way to establish the species belonging of muscle tissue fragments and particles using the quantitative solid-phase enzyme-linked immunosorbent assay method.Material and methods: We studied human muscle tissue fragments and particles (sampled 2-4 weeks before) and also dried extracts from the blood of certain animals, such as dogs, birds, pigs, and cats. The samples were studied using quantitative enzyme immunoassay with the domestic kit «IgG-total-ELISA-HEMA».Results: The study confirmed the method's sensitivity and specificity. We determined the optical density and concentration total IgG indicative values and selected the optimal values of the minimum and maximum calibration samples: 0.076±0.014-2.623±0.351.Conclusion: Experts from forensic biological units can use the obtained data when establishing the species belonging of muscle tissue particles using a quantitative solid-phase enzyme-linked immunosorbent assay method.","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 6","pages":"21-24"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Theory of formation of the postmortem muscle contraction ridge during mechanical stimulation of skeletal muscles].

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246706117

E V Tumanov, E M Kildyushov, D N Uslontsev

引用次数: 0

[Case of elongated styloid process detected in forensic medical examination of skeletal remains]. [骸骨法医鉴定中发现的伸长样式突病例]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246705151

O M Fandeeva, V L Popov, I V Gaivoronsky, A L Fandeev, G I Nichiporuk

引用次数: 0

[Modern opportunities of using computer programs and mobile devices in the frame of personality identification]. [在人格识别框架内使用计算机程序和移动设备的现代机遇]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246704127

O I Kosukhina, Yu P Kareva, S V Leonov, E N Leonova, K P Selyanina, Yu I Pigolkin

引用次数: 0

[Educational trajectory of forensic medical expert in the system of continuous medical education]. [继续医学教育体系中法医专家的教育轨迹]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246702153

G Kh Romanenko, T I Avdiukhina

引用次数: 0

[The study of the features of the determination and nature of the localization of 2-amino-4.6-dinitrophenol in the body of warm-blooded animals in case of lethal poisoning]. [研究致命中毒情况下 2-氨基-4.6-二硝基苯酚在温血动物体内定位的特征和性质]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246703139

V C Shormanov, N G Pogosyan, V A Omelchenko

{"title":"[The study of the features of the determination and nature of the localization of 2-amino-4.6-dinitrophenol in the body of warm-blooded animals in case of lethal poisoning].","authors":"V C Shormanov, N G Pogosyan, V A Omelchenko","doi":"10.17116/sudmed20246703139","DOIUrl":"https://doi.org/10.17116/sudmed20246703139","url":null,"abstract":"The aim of the work is to study the nature of the distribution of 2-A-4.6-DNP in the organisms of warm-blooded animals with intragastric administration of a toxicant. The study was carried out using the methods of TLC, UV-Visible spectroscopy, and GC-MS using derivatives of 2-A-4.6-DNP. Male Wistar rats at the age of 4 months were considered as a model of the body of a warm-blooded animal. An oily suspension of 2-A-4.6-DNF was administered intragastrically in an amount of three times the LD50. Extraction of the target substance from the biomaterial was carried out by double infusion (30 minutes each) with a mixture of acetone-acetonitrile (1:1), the amount of the mixture exceeded the weight of the biomaterial by 2 times. Extractions were purified by TLC method using «Sorbfil» plates and acetone-chloroform (7: 3) mobile phase. Preliminary identification was carried out at the same time using a standard substance. Confirmatory identification was carried out by the absorption of dimethylformamide eluates in «SF-2000», as well as by the retention time and mass spectra of the major compound of the corresponding chromatographic peaks after GC-MS analysis. The quantitative content was determined spectrophotometrically, in DMF, by optical density at the analytical wavelength (490 nm). 2-Amino-4.6-dinitrophenol was found unchanged in the blood and in all the studied hollow and parenchymal organs of poisoned rats. The largest amount of 2-amino-4.6-dinitrophenol (mg/100 g) was found in the stomach walls (199.39±25.43) and stomach contents (143.14±22.63), a significant amount of the substance was found in the heart (33.49±3.66), skeletal muscles (30.70±2.64), as well as in the spleen (24.30±1.96).","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 3","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141421242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Vibrational spectroscopy use for forensic purposes combined with machine learning]. [将振动光谱法用于法医目的与机器学习相结合]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246704169

R Alpuche, Yu I Pigolkin, S N Zakharov, I K Lednev

引用次数: 0

[Acute poisoning with antiseptic of external application containing chlorohexidine in the case of accidental intravenous administration causing serious harm to health]. [意外静脉注射含氯己定的外用消毒剂导致急性中毒，严重危害健康]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246703150

M Sh Mukashev, A E Turganbaev, B Toktosun Uulu, B A Asanov, G M Kalybekova, A B Ibragimov

{"title":"[Acute poisoning with antiseptic of external application containing chlorohexidine in the case of accidental intravenous administration causing serious harm to health].","authors":"M Sh Mukashev, A E Turganbaev, B Toktosun Uulu, B A Asanov, G M Kalybekova, A B Ibragimov","doi":"10.17116/sudmed20246703150","DOIUrl":"10.17116/sudmed20246703150","url":null,"abstract":"The article presents a case of expert evaluation of poisoning with chlorohexidine in the case of intravenous administration to a maternity woman who entered the maternity department for a planned cesarean section, contrary to the instructions for the use of this antiseptic, intended only for external or cavity application. The toxic effect of chlorohexidine began with thoracic discomfort, shortness of breath, then deep hypoxic phenomena in the form of diffuse cyanosis of the skin, facial pastoralism, respiratory disorders requiring ventilation, acute decrease of oxygen saturation in the blood up to 77% with further decrease to 60%, a pattern of pronounced hemorrhagic edema in the lungs, occurred when consciousness was absent. The consequences of poisoning are assessed as life-threatening from the forensic medical position, and uterus removal to prevent a heavy atonic hemorrhage is considered as loss of the organ. These features are characterized as causing serious harm to health. The legal evaluation of the health workers' actions is the prerogative of the juridical investigative authorities.","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 3","pages":"50-53"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141421215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[On improvement of legal regulation of personnel training in the sphere of state forensic medical activity]. [关于完善国家法医活动领域人员培训的法律规定]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246705162

I E Loban

引用次数: 0

[Development of selective method for identifying mebeverine in hair]. [开发鉴定毛发中美白藜芦醇的选择性方法]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246705129

P S Vikman, O L Balabanova, O Yu Strelova, A N Grebenyuk

{"title":"[Development of selective method for identifying mebeverine in hair].","authors":"P S Vikman, O L Balabanova, O Yu Strelova, A N Grebenyuk","doi":"10.17116/sudmed20246705129","DOIUrl":"https://doi.org/10.17116/sudmed20246705129","url":null,"abstract":"Objective: To develop the method for identification of mebeverine and its metabolites in biological materials (urine and hair) for solution of the problem of possible cross-reaction with psychoactive substances during preliminary studies by immunochromatographic method.Material and methods: The study was carried out using «Mebeverine» tablets (NAO «Severnaya Zvezda», Russia), enzymes, namely trypsin, chymotrypsin and hyaluronidase, papain. The obtained extracts were analyzed using HELC-MS/MS on a modular liquid Nexera XR chromatograph with a tandem LCMS-8050 (Shimadzu) mass spectrometer. Guinea pigs weighing about 500 gr were used in modelling the long-term use of mebeverine. The drug was administered per os, once to 5 guinea pigs at a dosage of 200 mg calculated as an animal weighing 500 gr and daily urine was collected. The wool was collected 30 days after regular administration.Results and conclusion: The main mebeverine's metabolites have been determined and native mebeverine and its marker metabolites (mebeverine and veratric acids) have been found in urine while taking this medicine in therapeutic doses for medical purposes. The application of enzymatic hydrolysis of hair method allowed to establish that hair tissue accumulates only a native molecule of mebeverine, therefore, only by results of hair analysis it is possible to conclude, if there was pharmacological use of this medicine or psychoactive substances from the group of phenylalkilamines. The effectiveness of enzymatic hydrolysis by hyaluronidase to isolate mebeverine from hair has been shown.","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 5","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0