Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia最新文献

Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples 肉类加工样品中猪DNA的实时PCR检测和定量

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284634

M. Baihaqi, Y. Rachmawati, Saiku Rokhim, M. Munir, L. Hamidah

{"title":"Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples","authors":"M. Baihaqi, Y. Rachmawati, Saiku Rokhim, M. Munir, L. Hamidah","doi":"10.4108/EAI.2-5-2019.2284634","DOIUrl":"https://doi.org/10.4108/EAI.2-5-2019.2284634","url":null,"abstract":"Meat mills in Indonesia are not halal certified. Small traders who do not have a food processor facility will choose the public market. This can be done both by traders who sell halal processed food and processed pork. It is necessary to study the content of meat mills in the public market so that it becomes critical evidence of an institution authorized to issue a policy immediately. This study aims to examine the content of porcine DNA in meat mills in Pasar Surya Kota Surabaya using the Real Time PCR (qPCR) method. QPCR amplification was performed using a porcine probe primer with annealing temperature optimization of 60oC. The amplification curve shows an increase in the curve in the positive control (EPC) with a value of Cq 34.00, while the negative control (NTC) is not amplified. The results of this study show that 2 out of 10 samples experienced an increase in the amplification curve on the FAM indicator, meaning that in the meat grinding sample there was a content of porcine DNA. The two samples are 8 and 9 with Cq values of 38.02 and 39.04, respectively. Market Halal Certification must be done immediately.","PeriodicalId":355290,"journal":{"name":"Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124934489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Biodegradation of Cocoa Pod Husk Waste used Rot Fungi Consortium at Various Storage Times 腐殖菌群在不同贮存时间对可可豆荚壳废弃物的生物降解研究

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284691

I. Rahim, J. Jumardin, S. Suherman, A. Nasruddin

{"title":"Biodegradation of Cocoa Pod Husk Waste used Rot Fungi Consortium at Various Storage Times","authors":"I. Rahim, J. Jumardin, S. Suherman, A. Nasruddin","doi":"10.4108/EAI.2-5-2019.2284691","DOIUrl":"https://doi.org/10.4108/EAI.2-5-2019.2284691","url":null,"abstract":"Cocoa pods is only considered as waste and was not utilized. Even though the utilization of cocoa pod husk waste was enough extensive, as a nutrient-rich fertilizer for plants. Degradation of cocoa pod husk waste naturally takes a long time, so could needed acceleration to degraded by using rot fungi. The purpose of this study was to determine the ability of rot fungi consortium to degraded cocoa pod husk waste at various time of storage. Then to know percentage of lignocellulotics, weight loss, C/N ratio, and the nutrient content produced from the biodegradation process. The cocoa pod husk waste that has been chopped were inoculated with a consortium of Mycena sp., Tremella sp., and Pleurotus sp.. and fermented for 40 days at room temperature. The results showed that the lowest percentage of weight loss was on fresh cocoa pod husk, and the highest weight loss up to 2.3% in cocoa pod husk which was stored 30 days. The highest levels of C and P on cocoa pod stored 90 days and fermented 60 days, respectively at 8.98% and 0.59%. It’s showed that the best cocoa pods for compost were 90 days stored. Consortium of Mycena sp., Tremella sp., and Pleurotus sp. was a biodegradator for plantation crops waste that has the potential to be developed.","PeriodicalId":355290,"journal":{"name":"Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia","volume":"185 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124698922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational Study of the Heat Ventilation on The Attics of Buginese Houses 商业住宅阁楼热通风的计算研究

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284620

S. Latif, B. Hamzah, R. Rahim, R. Mulyadi, I. Idrus

引用次数: 2

Dynamics of Macrofages and Cytokines after Myocardial Infarction 心肌梗死后巨噬细胞和细胞因子的动态变化

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284674

Usman Pagalay, Luluk Handayani, A. Azzam

引用次数: 1

Numerical analysis of Composite with Natural Fiber Reinforcement using Finite Element Method: Leaf Spring Composite Application 基于有限元法的天然纤维增强复合材料数值分析:钢板弹簧复合材料的应用

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284617

Amirin Kusmiran, Muhamad Hidayat, R. Desiasni, A. Maad

{"title":"Numerical analysis of Composite with Natural Fiber Reinforcement using Finite Element Method: Leaf Spring Composite Application","authors":"Amirin Kusmiran, Muhamad Hidayat, R. Desiasni, A. Maad","doi":"10.4108/EAI.2-5-2019.2284617","DOIUrl":"https://doi.org/10.4108/EAI.2-5-2019.2284617","url":null,"abstract":"Leaf spring is one of component that use to vibration damping on vehicle, where one of material that use to fabrication this component is 55Si7 steel. Currently, sustainable product is main focus in industry, so that composite is a potential alternative to replace steel as leaf spring. Materials that use to composite fabrication are sisal as fiber, epoxy as matrix, and honeycomb and alumunium alloy as core, where fabrication both 55Si7 steel and composite specimen are based on ASTM D638 standard. Fabrication of composite specimen conducted by hand lay-up technique, and mechanical properties analysis of thus specimen are conducted by finite element method. Tensile test is tested on 55Si7 steel and composite specimen using finite element method. According tensile test, steel of 55Si7 type have 2.92 mm of total deformation average, and 12583 MPa of strain, composite natural fiber with honeycomb core have 62.75 mm of total deformasi average, and 18590 MPa of stress, and composite natural fiber with aluminium alloy core have 15.47 mm of total deformasi average, and 12813 MPa of stress. According that results, composite with aluminium alloy core have the mechanical properties close to 55Si7 steel than composite honeycomb core.","PeriodicalId":355290,"journal":{"name":"Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114313117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Capsaicin Level and Anatomy Response of Curly Red Chili (Capsicum annuum L.) to Bio Fertilizer and Sludge Biogas Application

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284700

D. Siswanti, Utaminingsih Utaminingsih, Nurul Pangestuti

{"title":"Capsaicin Level and Anatomy Response of Curly Red Chili (Capsicum annuum L.) to Bio Fertilizer and Sludge Biogas Application","authors":"D. Siswanti, Utaminingsih Utaminingsih, Nurul Pangestuti","doi":"10.4108/EAI.2-5-2019.2284700","DOIUrl":"https://doi.org/10.4108/EAI.2-5-2019.2284700","url":null,"abstract":"Curly red chili (Capsicum annuum L.) is widely cultivated using inorganic fertilizers which causes high production cost. Chili is value because of the level of spiciness. The level of spiciness of chili is determined by the level of capsaicin which is affected by nutrients in the growing media. Bio fertilizer and sludge biogas which is one of the organic fertilizer containing microbes and organic material that are able to support nutrien of the medium. This study aimed to measure capsaicin level and analyze the anatomy response of fruits in curly red chili which given by bio fertilizer and sludge biogas in various dosage. The study design used RCBD, the data analyzed by ANOVA at 95% confidence level. The highest capsaicin level were obtain on curly red chili which were given sludge biogas 36 mL + bio fertilizer 10 L /ha. The combination treatment of sludge biogas and bio fertilizer give the highest anatomical response ie thickness of leaves, thickness of parenchyme palisade, diameter of stele, siameter of metaxylem and thickness of pericarp.","PeriodicalId":355290,"journal":{"name":"Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124257625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Vehicle Detection and Counting to Identify Traffic Density in The Intersection of Road Using Image Processing 基于图像处理的交叉口车辆检测与计数识别交通密度

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284706

Fitria Lahinta, Z. Zainuddin, S. Syarif

引用次数: 1

Determination of the Physical and Chemical Properties of the Complex Latosol Soil, Mediterranean, Alluvial and Its Inhibitory Effect on Dog Saliva Bacteria 复合红壤、地中海、冲积土理化性质测定及其对狗唾液细菌抑制作用

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284695

Sjamsiah Sjamsiah, Sappewali Sappewali, Miftahul Jannah, A. Usman, B. Nur, Arifuddin Ahmad, M. Masri, Indah Hasanuddin, H. Hamrullah

{"title":"Determination of the Physical and Chemical Properties of the Complex Latosol Soil, Mediterranean, Alluvial and Its Inhibitory Effect on Dog Saliva Bacteria","authors":"Sjamsiah Sjamsiah, Sappewali Sappewali, Miftahul Jannah, A. Usman, B. Nur, Arifuddin Ahmad, M. Masri, Indah Hasanuddin, H. Hamrullah","doi":"10.4108/EAI.2-5-2019.2284695","DOIUrl":"https://doi.org/10.4108/EAI.2-5-2019.2284695","url":null,"abstract":"This research is to determine the physical and chemical properties of LatosolComplex soil, Mediterranean and Alluvial. In addition, a resistor power test is conducted soil with liquid soap against dog saliva bacteria using a method of sumuran, is by measuring the diameter of the inhibitory zone. The result of this research is obtained Latosol Complex soil has a pH of 4-5, dry weight of 0.07% -0.09%, water content of 6% -7%, and N-total 0.08% -0.19%. Mediterranean soil has a pH of 4.8-5.0, dry weight of 0.06% -0.16%, water content 5.8% -6.6%, N-total 0.09% -0.2%. Alluvial land has pH 6.7-6.9, weight dry 0.03% -0.50%, water content 3.0% -3.9%, and N-total 0.6% -1.7%. Optimum inhibitory zone of wild dog saliva bacteria was obtained in succession the combination of 10% Latosol Complex soil with liquid soap is 2.4 cm. Mix 20% Mediterranean soil with liquid soap is 2.4 cm. Aluvial 60% with liquid soap is 2.5 cm. At depth of soil is 30 cm each.","PeriodicalId":355290,"journal":{"name":"Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129535833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Docking of Bawang Dayak (Eleutherine bulbosa) Secondary Metabolites as Bacterial Cell Wall Synthesis Inhibitor 霸王大黄次级代谢物作为细菌细胞壁合成抑制剂的分子对接

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284686

Mohammad Rizki Fadhil Pratama, I. Aziz

引用次数: 9

Screening Endophytic Fungi from Local Rice for Lignocellulolytic Enzyme Production 本地水稻内生真菌生产木质纤维素酶的筛选

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 2019-06-14 DOI: 10.4108/EAI.2-5-2019.2284633

S. Syamsia, Abubakar Idhan, Irma Hakim, A. Patappari, N. Noerfitryani

{"title":"Screening Endophytic Fungi from Local Rice for Lignocellulolytic Enzyme Production","authors":"S. Syamsia, Abubakar Idhan, Irma Hakim, A. Patappari, N. Noerfitryani","doi":"10.4108/EAI.2-5-2019.2284633","DOIUrl":"https://doi.org/10.4108/EAI.2-5-2019.2284633","url":null,"abstract":"Lignocellulose is a complex molecule consisting of lignin, cellulose, and hemicellulose was difficult to degrade. Microorganisms such as fungi and bacteria have the ability to produce lignocellulolytic enzymes that can degrade lignocellulose. This study aimed to isolate and screen endophytic fungi isolates from local rice which have the ability to produce lignocellulolytic enzymes. Entophytic fungi were isolated from roots, stems, and leaves of local rice. Fungi was isolated on Potato dextrose Agar (PDA) media. Screening of endophytic fungi for lignocellulolytic enzyme production by Czapek Dox Agar (CDA) media which have been added with reamazol brilliant blue (RBB), chitin, carboxymotin cellulose, pectin and, lignin as much as 0.1% respectively. The presence of clear zones around endophytic fungi isolates is an indicator of the ability to produce chitin, cellulose, pectin, and lignin. Successfully isolated 16 endophytic fungi isolates from Pulu Mandoti Local rice. The 16 endophytic fungus isolates have the different ability in producing lignocellulosic enzymes (cellulose, chitin, lignin and pectin). There were 3 isolates that have the highest ability in producing cellulose, chitin, lignin and pectin enzymes. These results relevant to explore endophytic fungi and has potential in degrading agricultural waste and controlling pests and","PeriodicalId":355290,"journal":{"name":"Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134217982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1