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Acta Microbiologica Bulgarica最新文献

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[The diagnosis of human papillomaviruses in cervical smears by deoxyribonucleic acid hybridization]. 脱氧核糖核酸杂交法诊断宫颈涂片中人乳头瘤病毒
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
A Mincheva, L Shindarov, B Slavchev, I Karag'ozov, V Makaveeva, N Vasilev
{"title":"[The diagnosis of human papillomaviruses in cervical smears by deoxyribonucleic acid hybridization].","authors":"A Mincheva,&nbsp;L Shindarov,&nbsp;B Slavchev,&nbsp;I Karag'ozov,&nbsp;V Makaveeva,&nbsp;N Vasilev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The method of filter in situ hybridization is applied for diagnostics of human papilloma viruses of the types 11, 16 and 18 in cervical smears [correction of frictions]. The results of the hybridization of 30 cell materials with clonal virus DNA probes proved the existence of human papilloma viruses in 15 patients. The precision of the filter in situ hybridization is demonstrated by comparing the results of the cytological study to the existence of HPV DNA in the same materials proved by in situ DNA hybridization.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"62-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13320467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The dissociation of a mutant strain of Streptomyces levoris 41-08, a producer of ichthiomycin]. [一种产鱼硫霉素的左旋链霉菌41-08突变株的分离]。
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
K Popov, I Ognianov, I Gameĭska, R Tsvetkova
{"title":"[The dissociation of a mutant strain of Streptomyces levoris 41-08, a producer of ichthiomycin].","authors":"K Popov,&nbsp;I Ognianov,&nbsp;I Gameĭska,&nbsp;R Tsvetkova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The population of Streptomyces levoris 41-08, producer of ichtiomicin, is established to be unhomogeneous and consisting of four types of morphologically different varieties: light grey, white, oligospore and asporogenous. The morphological varieties differ in their biochemical activity. The most promising for the ichtiomicine biosynthesis is the grey variant, some of whose representatives manifest up to 30% higher activity than the controls. The statistical processing of data shows that the oligospore and the asporogene variants with their limits of activity to 121.8 and 119.3% respectively are promising too, while the existence of white representatives in the population is not recommended since they are low-active minus variants.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"31-4"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13352720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Biochemical research on the neuraminidase of Erysipelothrix rhusiopathiae]. 红唇丹毒神经氨酸酶的生化研究
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
I Abrashev, P Velcheva
{"title":"[Biochemical research on the neuraminidase of Erysipelothrix rhusiopathiae].","authors":"I Abrashev,&nbsp;P Velcheva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purified neuraminidase preparation from Erysipelothrix rhusiopathiae is studied biochemically. The main physicochemical characteristics of the enzyme are determined: molecular mass, isoelectric point, electrophoretic mobility, chemical nature, UV spectrum, substrate specificity, etc. The obtained data are compared to those for other bacterial neuraminidases.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"3-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13531411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The immunostimulating action of suramin in infection with the infectious rhinotracheitis virus]. 苏拉明对传染性鼻气管炎病毒感染的免疫刺激作用。
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
V Dimov, A Toshkov, S Trifonova, N Khadzhiivanova, P Tsvetkov
{"title":"[The immunostimulating action of suramin in infection with the infectious rhinotracheitis virus].","authors":"V Dimov,&nbsp;A Toshkov,&nbsp;S Trifonova,&nbsp;N Khadzhiivanova,&nbsp;P Tsvetkov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The immunostimulating antiviral preparation suramin has a favourable effect on the process and outcome of an experimental infection with the virus of infectious rhinotracheitis (bovine herpes virus type 1, BHV1), thus reducing the gravity of the clinical course and increasing the survival rate. The therapeutic protective effect of the preparation is expressed in the prevention of virus-induced immunosuppression which is markedly strong during the acute phase of the infection process. Suramin restored the number, viability, relative share in the bronchial lavage population, phagocytic and microbicidal capacity of the alveolar macrophages--a major factor of the local defence mechanisms of the lung. In a similar way suramin influenced some parameters of the systemic immunity (number and differential count of polymorphonuclear phagocytes, plaque- and rosette-forming cells). The application of suramin in doses lower than those used for virus inhibition and its considerable action on different mechanisms of the immune system suggested that the preparation's therapeutic effect could be related mainly to its immunostimulating properties.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"26 ","pages":"10-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13253508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The thiobarbituric method for analyzing sugars. III. A method for studying sorbitol dehydrogenase activity]. 硫代巴比妥分析糖的方法3山梨醇脱氢酶活性的一种研究方法[j]。
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
L Lilov, Zh Saide, V Beshkov
{"title":"[The thiobarbituric method for analyzing sugars. III. A method for studying sorbitol dehydrogenase activity].","authors":"L Lilov,&nbsp;Zh Saide,&nbsp;V Beshkov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for studying of sorbitol dehydrogenase activity of Acetobacter suboxydans has been worked out which can be applied to other microbe cells too. The enzyme transformation of D-sorbitol into L-sorbose is determined through the reaction between ketohexose and 2-thiobarbituric acid under heating in 1 M oxalic acid (pH = 0.64) medium for 15 or 30 min. The yellow-coloured product was measured at 400 nm. The method is characterized with its high specificity, good reproducibility and accuracy, which allows for its application for quantitative analysis of sorbitol in dietetic foods. For this purpose a sorbitol dehydrogenase preparation isolated from bacterial cells is needed. The main purpose of the spectrophotometric method is to use it as a control in the process of microbial transformation of sorbitol into sorbose both under laboratory and industrial conditions.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"40-5"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13531412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Immunosuppression in an experimental infection with the infectious rhinotracheitis virus]. 传染性鼻气管炎病毒实验性感染的免疫抑制
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
V Dimov, E Slavcheva, A Toshkov, S Trifonova, N Khadzhiivanova, P Tsvetkov
{"title":"[Immunosuppression in an experimental infection with the infectious rhinotracheitis virus].","authors":"V Dimov,&nbsp;E Slavcheva,&nbsp;A Toshkov,&nbsp;S Trifonova,&nbsp;N Khadzhiivanova,&nbsp;P Tsvetkov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The changes in the immune status of the calves infected with bovine herpes virus-1 inducing infectious rhinotracheitis were studied. The virus was introduced by intratracheal inoculation of 5 cm3 suspension with minimal concentration of 10(-7) CPU50 per 1 cm2 from the Cervena voda strain cultivated in calf kidney culture. An infection of moderate seriousness and characteristic clinical manifestations was caused. On the 2nd, 5th, 10th and 15th day after the infection lung lavage was carried out and samples were taken from the peripheral blood. The number, viability and differential distribution of the alveolar macrophages and phagocytic and microbicidal ability showed a tendency to decrease during the acute phase of the infection (the 2nd-5th day) followed by a slow restoration in the period of convalescence (the 15th day). Some parameters of the systemic immunity--number of the plaque-forming and the rosette-forming cells, differential and absolute number of leucocytes in the peripheral blood--also had similar dynamics. The changes in the local and in the systemic, in the cell and humoral immunity were connected to the pathogenesis of the infection and the immune suppressive properties of the agent. The rhinotracheitis infection is indicative of the character of changes in the immune status at herpes virus infections and, on the other hand, it can be used as a model for testing immunostimulators of recovery effect.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"46-53"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13531413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The anti-influenza effect of rimantadine and isoprinosine when used in combination in mice]. [金刚乙胺和异丙氨酸在小鼠体内联合使用时的抗流感作用]。
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
S Pancheva, G Gegova, V Maksimova, N Manolova
{"title":"[The anti-influenza effect of rimantadine and isoprinosine when used in combination in mice].","authors":"S Pancheva,&nbsp;G Gegova,&nbsp;V Maksimova,&nbsp;N Manolova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The antiviral effect of rimantadine and isoprinosine applied in combination against fowl plague virus (FPV) in cell cultures of chick embryo fibroblasts and in experimental influenza infection in mice has been studied. Isoprinosine does not inhibit the reproduction of FPV when given alone and does not increase the antiviral activity of rimantadine. After oral application of both substances according to an appropriate scheme in mice infected with influenza virus A/Aichi (N3N2), an increase in the antiviral effect with an index of protection by 12% higher than the theoretically calculated one for additive effect was established. The results suggest that the combined effect is synergic.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"26 ","pages":"26-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12875079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The phage sensitivity and insecticidal activity of local strains of Bacillus sphaericus]. [球形芽孢杆菌本地菌株的噬菌体敏感性和杀虫活性]。
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
R Grigorova, T Khristova, A Abadzhieva, T Alchev, N Peneva, V Miteva, P Blagoev
{"title":"[The phage sensitivity and insecticidal activity of local strains of Bacillus sphaericus].","authors":"R Grigorova,&nbsp;T Khristova,&nbsp;A Abadzhieva,&nbsp;T Alchev,&nbsp;N Peneva,&nbsp;V Miteva,&nbsp;P Blagoev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The sensitivity of 132 local isolates of B. sphaericus to phages 3, 4 and SST of A. Yousten has been studied. According to this incomplete phage typing, most of the strains are classified in the third phage group. A high-degree correlation between sensitivity to phage 4 and insecticidal activity was established, owing to which this sensitivity can be used as an initial criterion for selection of newly isolated strains. LC50 and LC90 of one reference and six local strains of B. sphaericus were determined using Culex pipiens third instar larvae, and the local strains were found to exceed in insecticidal activity the reference strain 2362. This suggests that the newly isolated strains are promising for developing of a preparation for biological control of mosquitoes.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"26 ","pages":"77-83"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13253510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resistance of spores and vegetative cells of Streptomyces thermovulgaris strain 127 and the glucose isomerase produced by them to temperature and glutaraldehyde. 热乌氏链霉菌127孢子和营养细胞及其产生的葡萄糖异构酶对温度和戊二醛的抗性。
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
G Djejeva, Y Grigorova, M Stoychev
{"title":"Resistance of spores and vegetative cells of Streptomyces thermovulgaris strain 127 and the glucose isomerase produced by them to temperature and glutaraldehyde.","authors":"G Djejeva,&nbsp;Y Grigorova,&nbsp;M Stoychev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A study was made of the effects of high temperatures and glutaraldehyde on the spores and vegetative cells of Str. thermovulgaris strain 127. It was determined at what treatment times for different temperatures and glutaraldehyde concentrations the spore germination and vegetative cell growth stopped. A high heat stability was found for glucose isomerase, the loss in enzymatic activity increasing with the increase in glutaraldehyde concentration and exposure time. It was determined at what optimum parameters of the combined treatment the growth of the strain stopped and a comparatively high level of glucose isomerase activity was preserved.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13272237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The immunomodulating properties of a glucomacropeptide from whey. I. The stimulation of resistance in mice]. 乳清中糖宏肽的免疫调节特性。[1]刺激小鼠的抵抗力。
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
Kh Neĭchev, E Slavcheva, I Abrashev, P Todorova, I Mitov, D Veselinova
{"title":"[The immunomodulating properties of a glucomacropeptide from whey. I. The stimulation of resistance in mice].","authors":"Kh Neĭchev,&nbsp;E Slavcheva,&nbsp;I Abrashev,&nbsp;P Todorova,&nbsp;I Mitov,&nbsp;D Veselinova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The immunomodulating effect on mice of three products glucomacropeptides obtained from whey with different carbohydrate and peptide components has been studied. After the preliminary application of the preparations at several doses the protective effect in infections with Salmonella typhimurium and Klebsiella pneumoniae increases to different extent. At lower doses fraction 3 increases in vitro the mitogen-induced proliferation of the thymus cells. The phagocytosis of the polymorphonuclear cells on the 24th hour after application of the three preparations is activated. The influence of the structurally different fractions on the immunoprotective effect is discussed.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"54-61"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13352721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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