D Velianov, S Nikolova, Kh Naĭdenski, T Raducheva, V Kŭsovski, I Grigoriev, L Ilieva
{"title":"Studies on aerosol Yersinia pseudotuberculosis infection of guinea-pigs.","authors":"D Velianov, S Nikolova, Kh Naĭdenski, T Raducheva, V Kŭsovski, I Grigoriev, L Ilieva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Aerosol Y. pseudotuberculosis infection in guinea-pigs has been reproduced. In the course of infectious process, alveolar macrophages respiration is activated due to a rise in their cytochrome oxidase activity.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"30 ","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19272283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phosphatidylcholine-specific phospholipase C from Achromobacter xylosoxidans.","authors":"A Ivanov, S Kostadinova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new phosphatidylcholine-specific phospholipase C (EC 3.1.4.3.) has been isolated from the culture broth of Achromobacter xylosoxidans. Growth conditions were optimized for maximum production of the enzyme. A temperature-sensitive synthesis was established. Chromatography on CM Sephadex and polyacrylamide gel electrophoresis of the native enzyme revealed a number of isozymes. The water soluble substrate for phospholipase p-nitrophenylphosphorylcholine was not hydrolysed by the enzyme.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"29 ","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19494380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunological and biochemical investigations of infectious bovine rhinotracheitis. IV. Effect of suramin on pulmonary surfactant lipids.","authors":"N Khadzhiivanova, V Dimov, A Toshkov, E Filipova","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"30 ","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19272281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemiluminescent activity of macrophages during phagocytosis of different bacterial forms.","authors":"E Ivanova, V Dimov, S Bachev, J Gumpert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studies on the luminol-dependent chemiluminescent activity of rat peritoneal macrophages after in vitro interactions with E. coli WF+ L-form cells, their cytoplasmic membranes (CM) or parent bacterial cells were carried out. It was established that the phagocytosis of CM induce 20 time stronger light emission as compared to the L-form and 40 times compared to the parent bacterium cells respectively. Electron microscopical investigation of ultrathin sections of rat peritoneal macrophages after 24 h interaction in vitro with CM showed activation of the cell surfaces and vacuolisation of the cytoplasm. Inhibition of the phagolysosome fusion during phagocytosis of CM was observed. The mechanism of the immunostimulating activity of CM is discussed.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"29 ","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19494381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E Zvetkova, I Chowdhury, R Rusev, E Janeva, E Nikolova, I Tzenov, S Trifonov
{"title":"Influence of the immunomodulators suramin, MER and clenbuterol on the haemopoiesis of mice with virus-induced Rauscher leukemia.","authors":"E Zvetkova, I Chowdhury, R Rusev, E Janeva, E Nikolova, I Tzenov, S Trifonov","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"29 ","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19494382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dependence of the microbiological degradation of aryl-containing xenobiotics on their concentration.","authors":"R Dimkov, Y Topalova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of concentration of the toxic substrates: phenol, oNph, mNph, pNph on their microbiological degradation was studied. The dependence was investigated at biocenotic and population levels. The aryl-catabolic properties of 55 biodegrading cultures were studied at an increasing gradient of phenol substrates. The degradation dynamics has been studied at different initial phenol concentrations on 3 bacterial and one yeast strains.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"29 ","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19494386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Lazarova, V Kostov, S Zhelev, V Ivanova, T Sokoloov
{"title":"[The effect of acetic acid and furfural on yeast growth].","authors":"G Lazarova, V Kostov, S Zhelev, V Ivanova, T Sokoloov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acetic acid and furfural are normal components of the yeast hydrolysates. The influence of acetic acid in different concentrations (from 2 to 10 g/dm3) and furfural (from 0.2 to 0.8 g/dm3) on the growth of 8 yeast strains from the genus Candida under cultivation in synthetic nutrient media has been studied. It was established that some yeast strains absorb acetic acid better at pH 4.0-5.0, while others--only at pH 6.0 and at different concentrations of the acid in the nutrient media. Acetic acid in concentrations from 2 to 4 g/dm3 suppresses growth entirely in some strains, while in others growth was established. Acetic acid in concentrations over 8 g/dm3 inhibits growth in all 8 strains of the studied yeasts. Furforal in concentration to 0.4 g/dm3 in the synthetic nutrient media inhibits the growth in the 8 strains to a certain extent, while in concentration over 8 g/dm3 it stops the growth in all 8 yeast strains.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"24-30"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13531410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The synergistic antiviral effect of acyclovir and ribavirin against the herpes simplex type-1 virus and the pseudorabies virus in vitro].","authors":"S Shishkov, S Pancheva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The antiviral effect of the combination acyclovir (ACV) and ribavirin (R) on herpes simplex virus type 1 (HSV-1) in diploid cell culture of human embryonal skin and muscle fibroblasts and the virus of pseudorabies in secondary culture of chicken embryonal fibroblasts is studied. The antiviral activity is studied under the checker-board combinations of twofold dilutions of the two substances on the basis of inhibition of the virus cytopathic effect in microplates. The viruses are applied at 100 CCID50, per 0.1 cm3 in each well. Dose isobologrammes are made which point out to the synergistic type of interaction of the substances in the combination in relation to the two viruses. The concentrations with maximal synergistic effect in relation to HSV-1-1.1 for ACV and 41 mu for R respectively, and in relation to the virus of pseudorabies of 3.3 and 9.2 M respectively, are established.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13320468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Occurrence of Enterobacteriaceae and E. coli in the intestine of the newborn by caesarean section.","authors":"E Bezirtzoglou, C Romond","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been established that colonization of the gastrointestinal tract by Enterobacteriaceae and especially E. coli in newborn delivered by caesarean section occurs generally within the second day of life. On the 14th day, these bacteria inhabit the fecal flora of the majority of newborn. Hospital environment, as well as type of feeding do not seem to influence their frequency nor their level in the intestine of the infants born by caesarean section.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"76-81"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13352722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Protoplast reversion in Bacillus subtilis].","authors":"Ts Valerianov, A Kuiumdzhiev, A Ignatova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reversion of the protoplasts of two strains Bac. subtilis on 3 nutritive media: DP of Elliott et al., DM3 of Chang and Cohen and MRG of Bourne and Dancer, is studied. Special attention is devoted to the role of the components gelatin, bovine serum albumin and osmotic stabilizer. In relation to the conclusions drawn in the study a new medium for reversion of protoplasts is suggested--the glucitole reversion agar GRA ensuring continuous 20-40% reversion of the sown protoplasts.</p>","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"25 ","pages":"35-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13272236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}