木糖氧化无色杆菌的磷脂酰胆碱特异性磷脂酶C。

Q4 Biochemistry, Genetics and Molecular Biology

Acta Microbiologica Bulgarica Pub Date : 1993-01-01

A Ivanov, S Kostadinova

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引用次数: 0

摘要

从木氧化无色杆菌培养液中分离到一种新的磷脂酰胆碱特异性磷脂酶C (EC 3.1.4.3.)。优化生长条件，使酶的产量最大化。建立了一种温度敏感合成方法。对该天然酶进行CM Sephadex层析和聚丙烯酰胺凝胶电泳，发现了多个同工酶。磷脂酶对硝基苯基磷胆碱的水溶性底物不能被酶水解。

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Phosphatidylcholine-specific phospholipase C from Achromobacter xylosoxidans.

A new phosphatidylcholine-specific phospholipase C (EC 3.1.4.3.) has been isolated from the culture broth of Achromobacter xylosoxidans. Growth conditions were optimized for maximum production of the enzyme. A temperature-sensitive synthesis was established. Chromatography on CM Sephadex and polyacrylamide gel electrophoresis of the native enzyme revealed a number of isozymes. The water soluble substrate for phospholipase p-nitrophenylphosphorylcholine was not hydrolysed by the enzyme.

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来源期刊

Acta Microbiologica Bulgarica Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

0.40

自引率

0.00%

发文量