[枯草芽孢杆菌原生质体的还原]。

Q4 Biochemistry, Genetics and Molecular Biology
Acta Microbiologica Bulgarica Pub Date : 1990-01-01
Ts Valerianov, A Kuiumdzhiev, A Ignatova
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引用次数: 0

摘要

两个菌株原生质体的还原。研究了枯草芽孢杆菌在Elliott等人的DP、Chang和Cohen的DM3以及Bourne和Dancer的MRG三种营养培养基上的生长情况。特别注意了明胶、牛血清白蛋白和渗透稳定剂的作用。根据本研究得出的结论,提出了一种新的原生质体还原培养基——葡糖醇还原琼脂GRA,保证了播种原生质体的连续20-40%的还原。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Protoplast reversion in Bacillus subtilis].

The reversion of the protoplasts of two strains Bac. subtilis on 3 nutritive media: DP of Elliott et al., DM3 of Chang and Cohen and MRG of Bourne and Dancer, is studied. Special attention is devoted to the role of the components gelatin, bovine serum albumin and osmotic stabilizer. In relation to the conclusions drawn in the study a new medium for reversion of protoplasts is suggested--the glucitole reversion agar GRA ensuring continuous 20-40% reversion of the sown protoplasts.

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来源期刊
Acta Microbiologica Bulgarica
Acta Microbiologica Bulgarica Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
0.40
自引率
0.00%
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0
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