Acta Microbiologica Bulgarica最新文献_第5页

In Vitro Evaluation of Novel Bacillus-Derived Lipopeptide on Clinically Prevalent Trichophyton Spp. in Dermatophytosis 新型芽孢杆菌来源的脂肽对临床流行的毛癣菌的体外评价

Acta Microbiologica Bulgarica Pub Date : 2023-03-01 DOI: 10.59393/amb23390112

S. Ramesh, D. Shaw, M. Madhuri, S.M. Rudramurthy, U. Roy

{"title":"In Vitro Evaluation of Novel Bacillus-Derived Lipopeptide on Clinically Prevalent Trichophyton Spp. in Dermatophytosis","authors":"S. Ramesh, D. Shaw, M. Madhuri, S.M. Rudramurthy, U. Roy","doi":"10.59393/amb23390112","DOIUrl":"https://doi.org/10.59393/amb23390112","url":null,"abstract":"Trichophyton spp. are the leading cause of dermatophytosis globally, and in India. Antifungal li¬popeptides have emerged as potential candidates for developing alternative antifungal therapies. In our previous studies, the novel lipopeptide variant AF4, purified by reversed-phase HPLC from the wild-type Bacillus subtilis, demonstrated broad-spectrum antifungal activities against fungal clinical isolates. Here we have tested AF4 against Trichophyton spp. isolated from human samples to determine the antifungal susceptibility of T. rubrum, T. mentagrophytes complex and T. tonsurans. Twenty Trichophyton spp. isolates exhibited minimum inhibitory concentrations (MICs) in the range of 2-16 mg/L (100 % inhibition). The MICs of AF4 against six Trichophtyon spp. were equivalent to or less than that of MICs of terbinafine. AF4 has also exhibited potent activity against isolates of T. mentagrophytes complex and T. rubrum that carry mutations in the squalene epoxidase gene, which confers terbinafine resistance. In conclusion, we present a novel antifungal lipopeptide capable of significantly inhibiting the growth of Trichophyton spp. that is commonly found in clinical presentations of dermatophytosis.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135532399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association Between Naegleria fowleri and Bacteria, its Cytotoxicity and Protein Activity by Monosaccharides 福氏奈格氏杆菌与细菌的关系、细胞毒性及单糖蛋白活性

Acta Microbiologica Bulgarica Pub Date : 2023-03-01 DOI: 10.59393/amb23390110

S-Y. Jung

{"title":"Association Between Naegleria fowleri and Bacteria, its Cytotoxicity and Protein Activity by Monosaccharides","authors":"S-Y. Jung","doi":"10.59393/amb23390110","DOIUrl":"https://doi.org/10.59393/amb23390110","url":null,"abstract":"Naegleria fowleri is a brain-eating amoeba that inhabits freshwater and causes acute primary amoebic encephalitis (PAME). Studies on the importance of monosaccharides for the contact-dependent mechanism of free-living amoeba have been reported. In this study, association tests were analyzed to understand the contact-dependent mechanism of N. fowleri trophozoites with bacteria by monosaccharides. In addition, the cytotoxicity and proteolytic activity of N. fowleri by monosaccharides were analyzed. Salmonella typhi association by 100 mM mannose was inhibited by about 217% compared to the monosaccharide untreated control. The association of pathogenic S. typhi was about 180% higher than that of nonpathogenic Escher¬ichia coli. However, galactose, another monosaccharide, did not show the inhibitory effect of mannose. The cytotoxicity of the 50mM and 100mM mannose-treated groups was inhibited by 35.9% and 59.4%, respectively (P<0.05). On the other hand, in the 100 mM group treated with galactose, cytotoxicity was in¬hibited by about 25.8%. The activity of N. fowleri trophozoites proteolytic enzyme was maximized by 100 mM mannose but had little effect by galactose at a concentration of 0 to 100 mM. Taken together, it was confirmed that the pathogen-host interaction could be mediated by monosaccharides and that mannose and galactose could act as important mediators for N. fowleri trophozoites even though the galactose-induced proteolytic enzyme activity of N. fowleri trophozoites was weak.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"201 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135532400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial Properties and Evaluation of Survival in Model Systems of LAB Isolated from Oral Cavity 口腔分离LAB模型系统的有益特性及生存评估

Acta Microbiologica Bulgarica Pub Date : 2023-03-01 DOI: 10.59393/amb23390106

H. Kim, R. Rwubuzizi, J.I.I. Fugaban, W.H. Holzapfel, S.D. Todorov

{"title":"Beneficial Properties and Evaluation of Survival in Model Systems of LAB Isolated from Oral Cavity","authors":"H. Kim, R. Rwubuzizi, J.I.I. Fugaban, W.H. Holzapfel, S.D. Todorov","doi":"10.59393/amb23390106","DOIUrl":"https://doi.org/10.59393/amb23390106","url":null,"abstract":"In this study evaluation of the beneficial properties and survival for eight LAB putative probiotic strain was conducted including their ability to survive at different ox-bile concentrations and in the models representing different parts of digestive tract, ability to produce diacetyl, GABA and specific enzymes. The survival rates and release rates of the cells under the SSDP and AOS models were evaluated by encapsula¬tion using sodium alginate. Interaction between studied LAB and the pathogen in the artificial oral saliva was evaluated. Streptococcus salivarius ST48HK, ST59HK, ST61HK and ST62HK generated positive results for gadL and only Lactobacillus gasseri ST16HK for gadP, genes associated with GABA production. According to bio¬chemical approach in the evaluation of GABA production, strains Str. salivarius ST48HK, ST59HK, and Lactobacillus sakei ST69HK can be considered as the most promising producers, even if other evaluated strains showed levels of assimilation of sodium glutamate. Overall, tests for the beneficial properties, survival rate for the planktonic and encapsulated strains under different ox-bile conditions, SSDP, AOS models, indicates that the studied strains can be promising candidates for oral probiotics application. Evaluation of probiotic properties of new strains is a complex process, with essential basic requirement – survival in the different parts of digestive tract, stability for the extended storage time, expression of beneficial properties and safety characteristics. We have evaluated Str. salivarius ST48HK, ST59HK, ST61HK, and ST62HK, L. plantarum ST63HK and ST66HK, L. sakei ST69HK, and L. gasseri ST16HK as putative probiotics, promising candi-dates for complementary animals’ studies and future human applications.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135532403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hepatitis A and Hepatitis E Virus in Patients with Acute Hepatitis – a Three-Year Hospital-Based, Retrospective Study 急性肝炎患者的甲型肝炎和戊型肝炎病毒——一项基于医院的三年回顾性研究

Acta Microbiologica Bulgarica Pub Date : 2023-03-01 DOI: 10.59393/amb23390105

T. Kostadinova, Z. Stoykova, D. Tsaneva-Damyanova, T. Todorova

{"title":"Hepatitis A and Hepatitis E Virus in Patients with Acute Hepatitis – a Three-Year Hospital-Based, Retrospective Study","authors":"T. Kostadinova, Z. Stoykova, D. Tsaneva-Damyanova, T. Todorova","doi":"10.59393/amb23390105","DOIUrl":"https://doi.org/10.59393/amb23390105","url":null,"abstract":"Hepatitis A (HAV) and hepatitis E (HEV) are widespread hepatitis viruses causing usually acute he¬patic disease with benign outcome. The current study aims to investigate the etiological role of HAV and HEV in the hospitalized patients with symptoms of acute hepatitis. We retrospectively examined the num¬ber of anti-HEV IgM and anti-HAV IgM positive serum samples obtained for a three-year period (2016- 2018) in the Virology Laboratory of University Hospital St. Marina, Varna, Bulgaria. Anti-HEV IgM was detected in 16.0% and anti-HAV IgM – in 9.5% of the tested samples. Acute HEV affected mainly men (19.7% positive anti-HEV IgM male samples versus 10.3% female), while HAV was more prevalent in women (12.5% positive female samples versus 7.4% male). HAV infected mainly young individuals, while HEV predominated in older ages. Finally, the cases of HAV and HEV were distributed all year long but they are more prevalent in the cold months. HEV clearly dominates in the epidemiology of fecal-orally transmitted hepatitis among hospitalized patients in Varna. This shows the increasing importance of HEV for public health and the need for a specific nationwide measure to control the spread of the infection.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":"482 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135532402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amylase activity of Aspergillus strains--producers of organic acids. 有机酸生产者曲霉菌株的淀粉酶活性。

Acta Microbiologica Bulgarica Pub Date : 1993-01-01

K Tsekova, D Dentchev, A Vicheva, M Dekovska

引用次数: 0

Effectiveness of phenol biodegradation as a function of the physicochemical parameters of the medium (II). 苯酚生物降解效果与介质理化参数的函数关系(II)。

Acta Microbiologica Bulgarica Pub Date : 1993-01-01

Y Topalova, R Dimkov

引用次数: 0

Effect of C-6 on reverse transcriptase of some avian retroviruses. C-6对某些禽逆转录病毒逆转录酶的影响。

Acta Microbiologica Bulgarica Pub Date : 1993-01-01

R Stefanova, I Mikhaĭlov, S Vakarelova, I Gagov, L Karamucheva