Current Enzyme Inhibition最新文献

{"title":"Seed Oils as Tyrosinase Inhibitors for the Treatment of Hyperpigmentation","authors":"Lata Kothapalli, Tanuja Dhakane, Komal Bhosale, Asha Thomas","doi":"10.2174/0115734080257150231017094342","DOIUrl":"https://doi.org/10.2174/0115734080257150231017094342","url":null,"abstract":"Background: Hyperpigmentation is a growing problem worldwide among various skin diseases and directly relates to the quality of life. The factors causing hyperpigmentation may range from excess exposure to sunlight, pollution, underlying disease conditions, adverse effects of a medication, modified lifestyle, and oxidative stress. Treatment includes the use of hydroquinone, retinoids, corticosteroids, and kojic acid along with anti-inflammatory drugs. However, these medications are preferred for short-term treatment under acute conditions, while in the case of long-term treatment, botanical extracts are a safe choice for a majority of the population for the treatment of hyperpigmentation. Formulation with plant extracts/oil enriched with polyphenols, vitamin C, and vitamin E are reported to be safe and effective in inhibiting melanogenesis. Objective: Seed oils composed of polyphenols, vitamins and unsaturated fatty acids were selected to evaluate their antioxidant property and tyrosinase enzyme inhibitory activity. Methods: Grape seed oil (GSO), papaya seed oil (PSO), and carrot seed oil (CSO) were evaluated for their total phenolic and flavonoid content , in-vitro antioxidant activity was done using DPPH assay and anti-tyrosinase activity was done usingMushroomtyrosinaseinhibition assay. Result: Total phenolic content for PSO was 0.936 mg/gm. Gallic acid equivalent (GAE) was highest when compared to GSO and CSO, while CSO had a higher total flavonoid concentration, 0.945 mg/gm as quercetin equivalent (QE). Free radical scavenging activity was comparable to standard and tyrosinase inhibitory activity for grape and carrot seed oils were 80.10 % and 76.52 % at 100µg/ml, respectively and was comparable to kojic acid used as standard. Conclusion: The results obtained suggest that the oils can be formulated as a topical depigmenting product and used for skin care and skin glow","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135873984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Hepatoprotective Effects of L-Glu and NAC against CCl4-Induced Oxidative Damage in Rats. Biochemical and Histopathological Evaluation","authors":"Nataliya Salyha, Yuriy Salyha","doi":"10.2174/0115734080257975230922050816","DOIUrl":"https://doi.org/10.2174/0115734080257975230922050816","url":null,"abstract":"Background: The imbalance between free radical formation and antioxidant defence leads to the development of oxidative stress. The search for substances that would mitigate or prevent the effects of oxidative stress remains relevant. Objective: Our goal was to compare the antioxidant and mitigation effects of L-glutamic acid (LGlu) and N-acetylcysteine (NAC) alone or in combination using a battery of biomarkers of oxidative stress such as reduced glutathione (GSH) superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST) and lipid peroxidation, determined as a content of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARS). Histopathological examination of the liver was also performed. Methods: Experimental rats were divided into five experimental groups. Exp.1: was treated with CCl4 only, Exp. 2: was treated with CCl4/L-Glu, Exp. 3: was treated with CCl4/Glu/NAC. Exp. 4: was treated with CCl4/NAC, Control 5: served as the control rats. Results: These findings suggest that the CCl4 leads to oxidative stress by depleting the antioxidant enzyme activities and increasing peroxidation products. The studied biochemical parameters were altered by the introduction of CCl4, which was normalised (to one degree or another) by L-Glu, LGlu/ NAC and NAC treatment. Conclusion: The most remarkable protective effect was observed in groups of rats that were treated with L-Glu only. This conclusion was confirmed by histopathological findings which showed less severe hepatocellular necrosis, fibrosis and inflammation in CCl4/L- Glu and CCl4/L-Glu/NAC treated group, compared to the CCl4 group.","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135647381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgement to Reviewers","authors":"","doi":"10.2174/157340801903230915113602","DOIUrl":"https://doi.org/10.2174/157340801903230915113602","url":null,"abstract":"","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of Type 2 Diabetes Mellitus by Plant-derived Natural Dipeptidyl Peptidase-4 Inhibitors through Incretin Degradation Lowering Effect: An Updated Review","authors":"Hari Kishan, Manjusha Choudhary, Sachin Sharma, Prabhjeet Kaur Bamrah, Nitesh Choudhary","doi":"10.2174/1573408019666230912123312","DOIUrl":"https://doi.org/10.2174/1573408019666230912123312","url":null,"abstract":"Abstract: Glucagon-like peptide-1 and Glucose-dependent insulinotropic polypeptides are the most investigated gut peptides concerned with the biological glucose milieu. Early and late metabolism of incretin governs glucose homeostasis in diabetes mellitus. Dipeptidyl Peptidase-4, present in pancreatic alpha cells, is responsible for incretin degradation. Emerging biotechnological and molecular approaches established the pathophysiological role of Dipeptidyl Peptidase-4 and incretin in type 2 diabetes mellitus. Thus, various conventional synthetic Dipeptidyl Peptidase-4 inhibitors have been formulated, but they have serious adverse effects such as cancer, pancreatitis, cardiovascular risks, hepatic dysfunctions, etc. So, the concoction of a Dipeptidyl Peptidase-4 inhibitor entity with less or no severe adverse event becomes a need for society and medical corridor. Over the last two decades, natural or conventional herbal remedies have emerged as an alternate therapy for diabetes and treating its complications. This review summarized various plants (Emblica officinalis, Adenia viridiflora, Cleome droserifolia, Lens culinaris, Hedera nepalensis Melicope glabra, etc.) Dipeptidyl Peptidase-4 inhibitors, which have been preclinically proven for hyperglycemia treatment.","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135885461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into JNK Inhibition-based Strategy for the Treatment of Alzheimer's Disease","authors":"G. Zyuz’kov, L. Miroshnichenko, T. Polyakova, E. Simanina, Alexander Vasil `evich Chayikovskyi, Larisa Yur`evna Kotlovskaya","doi":"10.2174/1573408019666230816143357","DOIUrl":"https://doi.org/10.2174/1573408019666230816143357","url":null,"abstract":"\u0000\u0000Existing drugs for the treatment of Alzheimer's disease do not meet the basic requirements. Therefore, it is necessary to search for fundamentally new targets for the discovery of drugs for Alzheimer's disease (AD). When creating approaches to stimulate neurogenesis, such a search is promising to conduct as part of the development of a strategy for targeted regulation of intracellular signal transduction in regeneration-competent cells (RCCs). Moreover, the participation of c-Jun N-terminal kinases (JNK) in the regulation of the functions of the nervous tissue and neuroglial cells is known.\u0000\u0000\u0000\u0000The aim of the work was to study the effect of the JNK inhibitor (IQ-1S) on exploratory behavior and cognitive functions in aged mice (16-month-old male C57BL/6 mice as a model of AD) in combination with the dynamics of shifts in the RCCs functioning (nervous tissue progenitors: neural stem cells (NSCs) and neuronal-committed progenitors (NCPs); neuroglial cells: astrocytes, oligodendrocytes, microglial cells).\u0000\u0000\u0000\u0000The studies were carried out on male C57BL/6 mice aged 16 months as model of Alzheimer's disease. We studied the effect of the JNK inhibitor on exploratory behavior, conditioned reflex activity, and on the functioning of RCCs in the subventricular zone of the cerebral hemispheres (SVZ). NCPs and neuroglial cells of different types were obtained by immunomagnetic separation.\u0000\u0000\u0000\u0000We observed significant changes in exploratory behavior and impaired conditioned reflex activity in aged C57BL/6 mice. The administration of the JNK inhibitor led to a significant correction of age-related behavioral and memory disorders in aged mice. At the same time, against the background of JNK inhibition-based therapy, an increase in mitotic activity and the content of both NSCs and NCPs in the SVZ was noted. However, these shifts were more pronounced in committed precursors. The phenomenon of the inhibition of NSC specialization under the influence of a pharmacological agent was also revealed. In addition, the JNK inhibitor caused an increase in the secretion of neurotrophic growth factors by oligodendrocytes and microglial cells.\u0000\u0000\u0000\u0000The findings open up prospects for the development of JNK targeting-based approaches for the treatment of AD.\u0000","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44228308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery Phenolic Profiles and In vitro Antioxidants, Neuroprotective, Anti-Diabetic Activities of Extracts from of Algerian Plant: Calendula mo- nardii Boiss. & Reut","authors":"Zakaria Samai, N. Toudert, N. Dadda, T. Hamel, Farida Zakkad, Chaima Zerrad, Siham Boutemedjet, C. Bensouici, S. Djilani","doi":"10.2174/1573408019666230810115245","DOIUrl":"https://doi.org/10.2174/1573408019666230810115245","url":null,"abstract":"\u0000\u0000Calendula suffruticosa subsp. monardii (Boiss. & Reut.) Ohle, a medicinal plant from the Mediterranean region and, more precisely, from the extreme northeast of Algeria, is characterized by its wide use in the traditional medicine of the local population. This is what prompted us to investigate some pharmacological benefits such as anti-diabetes and Alzheimer's activities, in addition to anti- oxidant activity.\u0000 \u0000The effects of extraction methods and solvents on the amount of phenolic profiles and the biological ac- tivity of the different parts of this plant were studied, where the aim was to obtain a high extraction yield of bioactive compounds and consequently high biological activities.\u0000\u0000\u0000\u0000In vitro standard procedures were used to assess enzyme inhibitory activity (AChE, BChE, α- Amylase and α-Glucosidase) of Calendula monadii, and the antioxidant activity was assessed using the DPPH, ABTS, CUPRAC, Reducing power and Ferrous ions assays.\u0000\u0000\u0000\u0000When using ultrasound, a significant increase in the amounts of (TPC, TFC and TFlas) and an- tioxidant activity (DPPH, ABTS, CUPRAC, Reducing power and Ferrous ions cheating assay) in addi- tion to the inhibitory activity of enzymes (AChE, BChE, α-Amylase α-Glucosidase) was found, com- pared to the results of conventional extraction. Furthermore, the aqueous solvent of ethanol 70% is the very effective solvent for extraction compared to methanol 70% aqueous solvent.\u0000\u0000\u0000\u0000Based on these results, it can be said that this plant contains important biological activities, so it can be used in phytotherapy.\u0000","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44963260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural antioxidant extracts as anti-browning agents from Algerian date palm fruit","authors":"B. Wafa, Djeridane Amar, B. Noureddine, Yousfi Mohamed","doi":"10.2174/1573408019666230807161244","DOIUrl":"https://doi.org/10.2174/1573408019666230807161244","url":null,"abstract":"\u0000\u0000Enzymatic browning is a negative change that affects fruits and vegetables. This makes them unfit for consumption or reduces their shelf life and quality. This reaction alters the appearance, texture, fragrance, taste, and nutritional value of the food. In order for these products to be widely accepted by consumers, it is necessary to use natural compounds with anti-browning properties.\u0000\u0000\u0000\u0000The main objective of this study was to select effective extracts for the anti-browning (anti-polyphenol oxidase and anti-peroxidase), and antioxidant activities of by-products of Phoenix dactylifera L., Ghars variety, from Algeria.\u0000\u0000\u0000\u0000Hydro-methanolic extracts from the pedicel, perianth, and leaves of date palm were examined to evaluate the phenol and total flavonoid contents. Using spectrophotometric techniques, the antioxidant activities were assessed using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH●), 2, 2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) radical (ABTS●+) and Ferric Reducing Antioxidant Power (FRAP) assay, examined the enzyme inhibitory activity against polyphenol oxidase and peroxidase of Phoenix dactylifera L. extract.\u0000\u0000\u0000\u0000The range of total phenolic and flavonoid contents was 12.29 to 48.98 mg gallic acid equivalent/g dry matter and 2.83 to 15.07 mg rutin equivalent/g dry matter, respectively. The pedicel extracts showed significant antioxidant activity in the DPPH and FRAP tests (IC50 = 0.0057 ± 0.0010 mg/ml and FEAC = 1.1961 ± 0.0647) compared to other extracts. However, in the ABTS assay, the leaf extract exhibited an interesting potency (IC50 = 0.0020 ± 0.0001mg/ml). The study on the anti-browning activity of date palm by-product extracts showed that it inhibited the activity of peroxidase enzyme from date palm fruit.\u0000\u0000\u0000\u0000This work is the first time the potential of an extract from date palm by-products to enzymatically reduce the browning of date palms is presented. According to the results obtained, the different organs studied from the Ghars date palm, are a powerful natural antioxidant and may include natural compounds that retard browning by enzymes.\u0000","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48181691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Enzyme Inhibitors in Drug Discovery: Current Strategies and Future Prospects","authors":"Kuldeep Singh, J. Gupta, Devendra Pathak, Shivendra Kumar","doi":"10.2174/1573408019666230731113105","DOIUrl":"https://doi.org/10.2174/1573408019666230731113105","url":null,"abstract":"\u0000\u0000: Enzyme inhibitors are an important therapeutic agent in drug discovery, as they can be used\u0000to manipulate biochemical pathways to treat a wide range of diseases. Current strategies for enzyme inhibition involve the use of small molecule inhibitors, natural products, or peptide-based inhibitors. These inhibitors can be used to target and modulate the activity of enzymes, which in turn can affect the\u0000progression of a particular disease. The future of enzyme inhibitor-based drug discovery lies in the development of more selective and potent inhibitors, as well as the ability to target enzymes that are involved in multiple pathways. Additionally, there is significant potential for the use of enzyme inhibitors\u0000in combination with other therapeutic strategies, such as gene therapy. Finally, the development of novel computational strategies for the design and optimization of enzyme inhibitor-based drugs is an exciting area of research. Such strategies could lead to the discovery of new and more effective inhibitors,\u0000which would facilitate the development of improved treatments for a variety of diseases. Hence, this review focuses on some techniques for the discovery of enzyme inhibitors and their applications as various therapeutic agents.\u0000","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46850206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peanut Resveratrol Inhibits COX-2 in Ehrlich Ascites Carcinoma In vivo Model","authors":"Hamed A. Abosharaf, Aliaa Habib, Abdul Aziz Gad, Tarek M. Mohamed","doi":"10.2174/1573408019666230726142647","DOIUrl":"https://doi.org/10.2174/1573408019666230726142647","url":null,"abstract":"\u0000\u0000The primary goal of researchers interested in the field of oncology continues to be the development of a new anti-cancer medicine with minimal side effects. Due to their minimal toxicity and impressive performance, natural source-mediated anti-cancer treatments are attracting a lot of attention. Objective: The purpose of the current work was to extract and purify resveratrol from local Leguminosae, such as peanut, beans, cowpea, lupine, fava bean, and soybean, and then assess its cyclooxygenase-2 (COX-2) inhibition. The aim was then to evaluate the anticancer potential of extracted resveratrol individually or combined with doxorubicin against Ehrlich ascites carcinoma (EAC) model.\u0000\u0000\u0000\u0000Resveratrol was extracted and purified using a silica gel column. The inhibition study of extracted resveratrol was conducted against COX-2 in vitro. Then, the anti-proliferation impact of resveratrol alone or combined with doxorubicin was evaluated against the previously established EAC model. Apoptotic/anti-apoptotic genes and cell cycle arrest were investigated.\u0000\u0000\u0000\u0000After being extracted from peanuts, resveratrol inhibited COX-2 in vitro competitively with an inhibition constant (Ki) of 0.545 µM, which is extremely close to the theoretically predicted value (0.48 µM) from molecular docking. Further, resveratrol obviously inhibited COX-2 in vivo. Importantly, resveratrol was able to cause apoptosis by upregulating Bax and downregulating the anti-apoptotic gene Bcl-2, either by itself or in combination with doxorubicin. Additionally, resveratrol's ability to stop the cell cycle is evidence of its COX-2-inhibiting antiproliferative properties.\u0000\u0000\u0000\u0000Resveratrol exhibits anticancer potential via inhibition of COX-2, and it could be appropriate for combinational therapy in vivo.\u0000","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43499469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective and Antioxidant Activities of Algerian Punica granatum L. Peel Extracts","authors":"Naila Chaabna, Oumaima Naili, Nafissa Ziane, C. Bensouici, S. Dahamna, D. Harzallah","doi":"10.2174/1573408019666230626142826","DOIUrl":"https://doi.org/10.2174/1573408019666230626142826","url":null,"abstract":"\u0000\u0000Oxidative stress is implicated in many neurodegenerative disorders, such as Alzheimer’s disease. As an alternative, Punica granatum peels are widely used in the treatment of several ailments. To date, this is the first study on the anti-acetyl/butyrylcholinesterase effects of diethyl ether extract from Algerian pomegranate peels.\u0000\u0000\u0000\u0000This research aimed to evaluate the content of total phenolics, flavonoids, flavonols, and tannins in Punica granatum peel crude extract and its fraction diethyl ether, as well as to assess its antioxidant and neuroprotective activities.\u0000\u0000\u0000\u0000The total phenolic, flavonoid, flavonol, and tannin contents in the extracts were evaluated using spectrophotometric methods. The inhibitory effects on acetyl/butyrylcholinesterase were assessed using the in vitro models. In addition, several methods were used to investigate the antioxidant capacity of extracts, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) free radicals scavenging, phenanthroline, reducing power and cupric ion reducing antioxidant capacity (CUPRAC) assays.\u0000\u0000\u0000\u0000Phytochemical analysis revealed that the diethyl ether extract recorded the highest content of total phenolics, flavonoids, flavonols, and tannins with values of 643.3±9.96µgGAE/mg, 78.97±9.97µgQE/mg, 31.32±1.79µgQE/mg and 96.6±13.65µgTAE/mg, respectively. Moreover, the diethyl ether extract exhibited the strongest activity in the DPPH scavenging assay (IC50=3.36±0.11µg/mL), ABTS scavenging assay (IC50=1.46±0.10µg/mL), CUPRAC assay (CA0.5=3.62±0.36µg/mL), phenanthroline assay (CA0.5=1.04±0.10µg/mL), reducing power assay (CA0.5=4.14±0.32µg/mL), anti-acetylcholinesterase assay (IC50=114.86±13.66µg/mL), and anti-butyrylcholinesterase assay (IC50=147.00±10.41µg/mL).\u0000\u0000\u0000\u0000The present study has shown the extracts from Punica granatum peel to be a rich source of bioactive compounds, potent antioxidants, and cholinesterase inhibitors. They could be considered as natural agents that can be used in anti-neurodegenerative pharmaceutical formulations.\u0000","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46872606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}