Natural antioxidant extracts as anti-browning agents from Algerian date palm fruit

Q4 Pharmacology, Toxicology and Pharmaceutics

Current Enzyme Inhibition Pub Date : 2023-08-07 DOI:10.2174/1573408019666230807161244

B. Wafa, Djeridane Amar, B. Noureddine, Yousfi Mohamed

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引用次数: 0

Abstract

Enzymatic browning is a negative change that affects fruits and vegetables. This makes them unfit for consumption or reduces their shelf life and quality. This reaction alters the appearance, texture, fragrance, taste, and nutritional value of the food. In order for these products to be widely accepted by consumers, it is necessary to use natural compounds with anti-browning properties. The main objective of this study was to select effective extracts for the anti-browning (anti-polyphenol oxidase and anti-peroxidase), and antioxidant activities of by-products of Phoenix dactylifera L., Ghars variety, from Algeria. Hydro-methanolic extracts from the pedicel, perianth, and leaves of date palm were examined to evaluate the phenol and total flavonoid contents. Using spectrophotometric techniques, the antioxidant activities were assessed using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH●), 2, 2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) radical (ABTS●+) and Ferric Reducing Antioxidant Power (FRAP) assay, examined the enzyme inhibitory activity against polyphenol oxidase and peroxidase of Phoenix dactylifera L. extract. The range of total phenolic and flavonoid contents was 12.29 to 48.98 mg gallic acid equivalent/g dry matter and 2.83 to 15.07 mg rutin equivalent/g dry matter, respectively. The pedicel extracts showed significant antioxidant activity in the DPPH and FRAP tests (IC50 = 0.0057 ± 0.0010 mg/ml and FEAC = 1.1961 ± 0.0647) compared to other extracts. However, in the ABTS assay, the leaf extract exhibited an interesting potency (IC50 = 0.0020 ± 0.0001mg/ml). The study on the anti-browning activity of date palm by-product extracts showed that it inhibited the activity of peroxidase enzyme from date palm fruit. This work is the first time the potential of an extract from date palm by-products to enzymatically reduce the browning of date palms is presented. According to the results obtained, the different organs studied from the Ghars date palm, are a powerful natural antioxidant and may include natural compounds that retard browning by enzymes.

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阿尔及利亚椰枣果实的天然抗氧化提取物抗褐变研究

酶促褐变是一种影响水果和蔬菜的负面变化。这使得它们不适合食用或降低了它们的保质期和质量。这种反应会改变食物的外观、质地、香味、味道和营养价值。为了使这些产品被消费者广泛接受，有必要使用具有抗褐变性能的天然化合物。本研究的主要目的是从阿尔及利亚筛选具有抗褐变（抗多酚氧化酶和抗过氧化物酶）和抗副产物抗氧化活性的有效提取物。对椰枣茎、花被和叶的水-甲醇提取物进行了酚类和总黄酮含量的测定。采用分光光度法，用1，1-二苯基-2-苦基肼基（DPPH●), 2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）自由基（ABTS●+) 和铁还原抗氧化能力（FRAP）测定法检测了凤爪兰提取物对多酚氧化酶和过氧化物酶的抑制活性。总酚和类黄酮含量的范围分别为12.29至48.98 mg没食子酸当量/g干物质和2.83至15.07 mg芦丁当量/g干物。与其他提取物相比，椎弓根提取物在DPPH和FRAP测试中显示出显著的抗氧化活性（IC50=0.0057±0.0010 mg/ml和FEAC=1.1961±0.0647）。然而，在ABTS测定中，叶提取物表现出令人感兴趣的效力（IC50=0.0020±0.0001mg/ml）。对椰枣副产物提取物的抗褐变活性的研究表明，它抑制了椰枣果实中过氧化物酶的活性。这项工作首次展示了从椰枣副产品中提取的提取物在酶促减少椰枣褐变方面的潜力。根据所获得的结果，从加尔斯椰枣中研究的不同器官是一种强大的天然抗氧化剂，可能包括通过酶延缓褐变的天然化合物。

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.