Seed Oils as Tyrosinase Inhibitors for the Treatment of Hyperpigmentation

Q4 Pharmacology, Toxicology and Pharmaceutics

Current Enzyme Inhibition Pub Date : 2023-11-03 DOI:10.2174/0115734080257150231017094342

Lata Kothapalli, Tanuja Dhakane, Komal Bhosale, Asha Thomas

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引用次数: 0

Abstract

Background: Hyperpigmentation is a growing problem worldwide among various skin diseases and directly relates to the quality of life. The factors causing hyperpigmentation may range from excess exposure to sunlight, pollution, underlying disease conditions, adverse effects of a medication, modified lifestyle, and oxidative stress. Treatment includes the use of hydroquinone, retinoids, corticosteroids, and kojic acid along with anti-inflammatory drugs. However, these medications are preferred for short-term treatment under acute conditions, while in the case of long-term treatment, botanical extracts are a safe choice for a majority of the population for the treatment of hyperpigmentation. Formulation with plant extracts/oil enriched with polyphenols, vitamin C, and vitamin E are reported to be safe and effective in inhibiting melanogenesis. Objective: Seed oils composed of polyphenols, vitamins and unsaturated fatty acids were selected to evaluate their antioxidant property and tyrosinase enzyme inhibitory activity. Methods: Grape seed oil (GSO), papaya seed oil (PSO), and carrot seed oil (CSO) were evaluated for their total phenolic and flavonoid content , in-vitro antioxidant activity was done using DPPH assay and anti-tyrosinase activity was done usingMushroomtyrosinaseinhibition assay. Result: Total phenolic content for PSO was 0.936 mg/gm. Gallic acid equivalent (GAE) was highest when compared to GSO and CSO, while CSO had a higher total flavonoid concentration, 0.945 mg/gm as quercetin equivalent (QE). Free radical scavenging activity was comparable to standard and tyrosinase inhibitory activity for grape and carrot seed oils were 80.10 % and 76.52 % at 100µg/ml, respectively and was comparable to kojic acid used as standard. Conclusion: The results obtained suggest that the oils can be formulated as a topical depigmenting product and used for skin care and skin glow

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种子油作为酪氨酸酶抑制剂治疗色素沉着

背景:色素沉着是世界范围内各种皮肤病中日益严重的问题，并直接关系到生活质量。引起色素沉着的因素可能包括过度暴露在阳光下、污染、潜在的疾病状况、药物的不良影响、改变的生活方式和氧化应激。治疗包括使用对苯二酚、类维生素a、皮质类固醇和曲酸以及抗炎药物。然而，在急性情况下，这些药物是短期治疗的首选，而在长期治疗的情况下，植物提取物是大多数人治疗色素沉着过度的安全选择。据报道，含有富含多酚、维生素C和维生素E的植物提取物/油的配方在抑制黑色素形成方面是安全有效的。目的:选择由多酚类、维生素和不饱和脂肪酸组成的种子油，评价其抗氧化性能和酪氨酸酶抑制活性。方法:测定葡萄籽油(GSO)、木瓜籽油(PSO)和胡萝卜籽油(CSO)的总酚和类黄酮含量，采用DPPH法测定其体外抗氧化活性，采用蘑菇酪氨酸抑制法测定其抗酪氨酸酶活性。结果:PSO总酚含量为0.936 mg/gm。GSO和CSO的没食子酸当量(GAE)最高，而CSO的总黄酮浓度为0.945 mg/gm，为槲皮素当量(QE)。在100µg/ml浓度下，葡萄籽油和胡萝卜籽油的酪氨酸酶抑制活性分别为80.10%和76.52%，与以曲酸为标准的酪氨酸酶抑制活性相当。结论:该精油可配制成局部脱色产品，用于皮肤护理和皮肤光泽

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.