Journal of Chromatography A最新文献_第6页

Direct ion chromatographic method for speciation micro analysis of arsenic forms in industrial samples with “rich” matrix composition 采用直接离子色谱法对基质成分 "丰富 "的工业样品中的砷形态进行微量标本分析。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-10-05 DOI: 10.1016/j.chroma.2024.465421

{"title":"Direct ion chromatographic method for speciation micro analysis of arsenic forms in industrial samples with “rich” matrix composition","authors":"","doi":"10.1016/j.chroma.2024.465421","DOIUrl":"10.1016/j.chroma.2024.465421","url":null,"abstract":"<div><div>The speciation analysis of arsenic has consistently been a subject of great interest. However, it remains challenging to analyze complex matrix samples that contain both arsenic and interfering components. In this case, it can be hard to choose the right combinations of different instrumental methods, or a separation method followed by detection, which is usually done using a spectral approach (hybrid methods). In the production control of copper electrorefining, the determination of the concentration of As (III) and As (V) helps to improve the quality of the cathode copper produced. This work investigated the possibility of directly determining both arsenic forms and total As in an electrolyte bath using ion chromatography (IC) with conductometric detection. The use of the ion chromatographic approach for the determination of As(V) in complex matrix samples such as copper electrolyte must take into account the presence of potential interferences from anions such as sulphates, sulfites, selenites, selenates, etc. The results revealed that the method is accurate and precise, with As(V) quantification limits of 15 µg.L<sup>-1</sup> and detection limits of 5 µg.L<sup>-1</sup>. This method is suitable for assessing various types of arsenic in the production of electrolytic copper, with the aim of replacing the current technique that requires liquid-liquid extraction and ICP-OES detection. This led to the following improvements: Enhanced efficiency: The method eliminates the need for extensive and time-consuming sample preparation for the initial separation of arsenic forms. At the same time, the method's characteristics are comparable to those of ICP-OES with liquid-liquid extraction, which is often used in the speciation analysis of arsenic. The method is environmentally friendly as it avoids the use of organic and poisonous extractants. The method can simultaneously analyze other anions (PO<sub>4</sub><sup>3-</sup>, SO<sub>4</sub><sup>2-</sup>, F<sup>-</sup>, Cl<sup>-</sup>, etc.) with arsenates with appropriate calibration.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating BTEX in vehicle exhaust gas: A fast and efficient approach using SPME and GC-BID 评估汽车尾气中的 BTEX：使用 SPME 和 GC-BID 的快速高效方法。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-10-05 DOI: 10.1016/j.chroma.2024.465417

{"title":"Evaluating BTEX in vehicle exhaust gas: A fast and efficient approach using SPME and GC-BID","authors":"","doi":"10.1016/j.chroma.2024.465417","DOIUrl":"10.1016/j.chroma.2024.465417","url":null,"abstract":"<div><div>Benzene, toluene, ethylbenzene, and the xylene isomers (m, p, and o-xylene) (BTEX) are known for their harmful effects on human health and have been extensively studied across various environmental matrices. However, quantifying BTEX in exhaust gases poses challenges due to the complexity of the matrices. In this study, we investigated a method development strategy involving solid-phase microextraction (SPME) and gas chromatography coupled with a dielectric barrier discharge ionization Detector (BID) for quantifying BTEX emitted from internal combustion engines operating at idle. Sampling was conducted using 1.0 L Tedlar bags, followed by withdrawal of aliquots and dilution with atmospheric air using a novel device (graduated vial) designed for gaseous samples. The SPME-GC-BID method was developed and validated for the conditions: BTEX extraction in CAR/PDMS 75 μm fiber at a contact time of 5.0 min at a temperature of 27 °C, followed by GC-BID analysis. Method validation to ensure the reliability of quantitative results used the merit figures e.g., limits of detection (LOD) and quantification (LOQ), precision, and accuracy (recovery). LOD varied from 0.194 to 0.340 mg <em>m</em><sup>−3</sup>, LOQ varied from 0.587 to 1.03 mg <em>m</em><sup>−3</sup>, precision ranged from 1.47 to 7.14 %, and recovery varied from 82.34 to 109.5 %. BTEX concentration in vehicle exhaust varied from 3.40 to 16.4 mg <em>m</em><sup>−3</sup>. The results showed, concerning the figures of merit analyzed, that the SPME-GC-BID method provides good sensibility, precision, and accuracy for evaluating the presence of BTEX in the exhaust of internal combustion engines, contributing to the understanding of health risks associated with vehicle emissions.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recoverable and reusable light-induced multi-arm azobenzenes-Fe3O4 hybrid sorbent for enrichment of phthalate plasticizer and utilized as a SALDI substrate for the detection of 2-naphthol 可回收和重复使用的光诱导多臂偶氮苯-Fe3O4 混合吸附剂，用于富集邻苯二甲酸酯类增塑剂，并用作检测 2-萘酚的 SALDI 底物。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-10-05 DOI: 10.1016/j.chroma.2024.465418

{"title":"Recoverable and reusable light-induced multi-arm azobenzenes-Fe3O4 hybrid sorbent for enrichment of phthalate plasticizer and utilized as a SALDI substrate for the detection of 2-naphthol","authors":"","doi":"10.1016/j.chroma.2024.465418","DOIUrl":"10.1016/j.chroma.2024.465418","url":null,"abstract":"<div><div>The construction, structural identifications along with compositional properties, using TEM, FT-IR, and XPS spectroscopies, of an innovative light-induced multi-arm azobenzenes based Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles (<strong>Azo-Fe<sub>3</sub>O<sub>4</sub> MNPs</strong>) are being reported. Such organic (light-sensitive dendrimers-like structure), inorganic (magnet core), hybrid material has been applied as an efficient recoverable/reusable extractive sorbent for the detection of phthalate plasticizers from acetate buffer solution. The extraction study was controlled within consecutive procedures <em>via</em> UV-light exposure to achieve pore-size control which then further subjected for the evaluation of the analytes’ retention, separation, and release as well as the detection of the phthalate pollutants using GCMS–. Various experimental conditions, such as time of extraction, salt concentration, pH, and desorption time, were studied and adjusted. Additionally, the extraction repeatability (RSD from 0.46 % to 6.12%, <em>n</em> = 5) of the studied sorbent was comparable to other published work. The linear range extended from 6.25 to 100 μg L<sup>-</sup>¹ and detection limits (LOD) within the range of 41- 150 ng L<sup>-1</sup> were achieved, demonstrating good linearity with values ranging from 0.9992 to 0.9892. The inter-batch and intra-batch RSD ranged from 0.46 % to 6.12 %, respectively. Additionally, it provides effective detection of 2-naphthol when used as a SALDI substrate.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zirconium(IV) coordination-mediated rapid and versatile post-modification of polydopamine coating as stationary phase for open-tubular capillary electrochromatography 锆（IV）配位介导的聚多巴胺涂层快速、多功能后改性，作为开放管式毛细管电色谱的固定相。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-10-02 DOI: 10.1016/j.chroma.2024.465415

{"title":"Zirconium(IV) coordination-mediated rapid and versatile post-modification of polydopamine coating as stationary phase for open-tubular capillary electrochromatography","authors":"","doi":"10.1016/j.chroma.2024.465415","DOIUrl":"10.1016/j.chroma.2024.465415","url":null,"abstract":"<div><div>In recent years, mussel-inspired polydopamine (PDA)-based materials have attracted significant attention in the field of open-tubular capillary electrochromatography (OT-CEC) owing to their diverse and appealing properties. However, previously established functionalized PDA coating-based CEC stationary phases predominantly relied on the latent reactivity of PDA with amine/thiol-containing molecules, limiting the types of applicable modifiers and requiring time-consuming reaction processes. Herein, we presented a versatile and efficient method for the facile and rapid fabrication of diverse functionalized PDA coatings as OT-CEC stationary phases through a Zr(IV) coordination-mediated post-modification strategy. Different kinds of modifiers, including octadecylamine (ODA), lauric acid (LA), and perfluorooctanoic acid (PFOA), were rapidly and robustly grafted onto the PDA coating, verified through multiple characterization techniques. The influences of preparation parameters on the grafting efficiency of the functionalized PDA coating were systematically investigated. Utilizing the Zr(IV)-mediated ODA-, LA- and PFOA-functionalized PDA-based OT-CEC columns, we achieved high-efficiency baseline separation of a series of neutral analytes with excellent repeatability, good stability, and long lifetime. Given the strong universality of the Zr(IV) coordination-mediated post-modification approach, our study provides an effective pathway for advancing the development of a wider range of functional PDA-based chromatographic stationary phases.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide retention time prediction for electrostatic repulsion-hydrophilic interaction chromatography 静电排斥-亲水相互作用色谱法的肽保留时间预测。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-10-02 DOI: 10.1016/j.chroma.2024.465414

{"title":"Peptide retention time prediction for electrostatic repulsion-hydrophilic interaction chromatography","authors":"","doi":"10.1016/j.chroma.2024.465414","DOIUrl":"10.1016/j.chroma.2024.465414","url":null,"abstract":"<div><div>Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) is one of the legacy separation tools developed by Dr. Andrew Alpert and has been used for developing unique separation methods of hydrophilic compounds, including peptides. In the past it has been studied using designed peptide libraries to elucidate major features of its separation mechanism, while comprehensive peptide retention modeling for ERLIC is still lacking. In this work we employed a proteomics-derived ∼170,000 peptide retention datasets to evaluate major ERLIC retention features using the framework of our Sequence-Specific Retention Calculator model. The separation conditions were adjusted to obtain a wider proteome coverage, particularly for non-modified peptides, resulting in a superior separation orthogonality for a 2D LC combination with reversed-phase C18 LC-MS in the second dimension. The SSRCalc ERLIC model presents a consistent theme with the existing ERLIC retention mechanism, reflecting a dependence on peptide orientation and the position of charged and hydrophilic residues across the peptide backbone. R<sup>2</sup> values of 0.935 and 0.955 accuracy were demonstrated for the standard interpretable SSRCalc model and machine learning algorithm, respectively. The effects of various PTMs on peptide retention were evaluated in this study, covering spontaneous (oxidation, deamidation) and enzymatic (N-terminal acetylation, phosphorylation, glycosylation) modifications.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple method to remove polyimide coating from fused silica capillaries for capillary electrophoresis 从毛细管电泳用熔融石英毛细管上去除聚酰亚胺涂层的简单方法。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-10-01 DOI: 10.1016/j.chroma.2024.465395

引用次数: 0

An explore method for quick screening biomarkers based on effective enrichment capacity and data mining 基于有效富集能力和数据挖掘的生物标记物快速筛选探索方法。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-10-01 DOI: 10.1016/j.chroma.2024.465413

{"title":"An explore method for quick screening biomarkers based on effective enrichment capacity and data mining","authors":"","doi":"10.1016/j.chroma.2024.465413","DOIUrl":"10.1016/j.chroma.2024.465413","url":null,"abstract":"<div><div>Protein glycosylation acts as a crucial role in regulating protein function and maintaining cellular homeostasis. Efficient peptide enrichment can be utilized to effectively solve the inherent challenges of protein glycosylation analysis to search unknown cancer biomarkers. In this research, a low dimensional porous hydrophilic nanosheets with a multi-level porous structure (Co-MOF-SiO<sub>2</sub>@HA) was synthetized via an easy one-pot method for the efficient enrichment of the N-glycopeptides in the digests of complex biosamples. The synthetized nanosheets Co-MOF-SiO<sub>2</sub>@HA demonstrated excellent enriching performances including a high enrichment capacity (300 mg g<sup>-1</sup> calculated), a spectacular selectivity (IgG digests and BSA digests at the molar ratio of 1/1200), and an excellent spatial confinement ability (IgG digests, IgG and BSA at the molar ratio of 1/1000/1000). As an explore result, after the enrichment of human colorectal cancer tissue and human healthy tissue by the nanosheets, several proteins related to cancers and one protein directly related to well-known human colorectal cancer were identified by detecting the corresponding glycopeptides. It presented the potential value of the feasibility of this analysis mode by nanosheets Co-MOF-SiO<sub>2</sub>@HA in proteomic analysis.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robustness evaluation of weak anion exchange chromatography method for the purity analysis of therapeutic oligonucleotides 用于治疗性寡核苷酸纯度分析的弱阴离子交换色谱法的稳健性评估。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-09-30 DOI: 10.1016/j.chroma.2024.465412

引用次数: 0

Magnetic covalent organic framework as an absorbent coupled with a portable mass spectrometer for rapid detection of malachite green and its metabolite residues 磁性共价有机框架作为吸收剂与便携式质谱仪联用，用于快速检测孔雀石绿及其代谢物残留。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-09-30 DOI: 10.1016/j.chroma.2024.465407

{"title":"Magnetic covalent organic framework as an absorbent coupled with a portable mass spectrometer for rapid detection of malachite green and its metabolite residues","authors":"","doi":"10.1016/j.chroma.2024.465407","DOIUrl":"10.1016/j.chroma.2024.465407","url":null,"abstract":"<div><div>It is important to develop specific adsorbents for malachite green and other fish drug residues. Herein, a simple strategy for synthesizing a novel magnetic covalent organic frameworks (rFe<sub>3</sub>O<sub>4</sub>@Py-COF) has been studied, and the materials were used as a magnetic absorbent for solid phase extraction (MSPE) of malachite green (MG) and its metabolite as leucomalachite green (LMG) in fishes. In this study, the mild reduction program of formic acid replacing traditional sodium borohydride as a reducing agent has been adopted to increase the stability of the framework, which can maintain the original high crystallinity and surface area of the reduced COF. The secondary amine bond is expected to be used as the reaction center for further functionalization of COF pore wall. Subsequently, rFe<sub>3</sub>O<sub>4</sub>@Py-COF (rmCOF) obtained after reduction was used as MSPE materials to detect MG and LMG by a portable mass spectrometer. After optimizing the conditions, the linearity is good within the range of 1.25∼100 μg/kg (R<sup>2</sup>≥0.9954), the limits of detection (LODs) are 0.31∼0.44 μg/kg with satisfactory recovery (85.0 %∼106.0 %). These results indicate that the assay is suitable for monitoring MG and LMG in complex aquatic foods, providing protection for food safety.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The emerging application of LC-based metallomics techniques to unravel the bioinorganic chemistry of toxic metal(loid)s 基于液相色谱的金属组学技术在揭示有毒金属（loid）的生物无机化学方面的新兴应用。

IF 3.8 2区化学

Journal of Chromatography A Pub Date : 2024-09-30 DOI: 10.1016/j.chroma.2024.465409

{"title":"The emerging application of LC-based metallomics techniques to unravel the bioinorganic chemistry of toxic metal(loid)s","authors":"","doi":"10.1016/j.chroma.2024.465409","DOIUrl":"10.1016/j.chroma.2024.465409","url":null,"abstract":"<div><div>The on-going anthropogenic emission of toxic metal(loid) species into the environment contaminates the food supply and drinking water resources in various parts of the world. Given that inorganic pollutants cannot be degraded, their increased influx into the bloodstream of babies, children and pregnant women is inevitable. Since the ramifications of the ensuing environmental exposure on human health remain poorly defined, fundamentally new insight into their bioinorganic chemistry in organisms is urgently needed. Based on the flow of dietary constituents through organisms, the interaction of toxic metal(loid) species with biomolecules in the bloodstream deserve particular attention as they play an integral role in the mechanisms of their chronic toxicity. Gaining insight into these bioinorganic processes is hampered by the biological complexity of plasma/red blood cells and the low concentrations of the metal(loid) species of interest, but can be overcome by employing LC techniques hyphenated to atomic spectroscopic detectors (i.e. metallomics techniques). This perspective aims to highlight the potential of unconventional hyphenated separation modes to advance our understanding of the bioinorganic chemistry of toxic metal(loid) species in the bloodstream-organ system. Four examples are illustrated. The application of anion-exchange (AEX) and size-exclusion chromatography (SEC) provided new insight into the blood-based bioinorganic mechanisms that direct Cd<sup>2+</sup> and MeHg<sup>+</sup> to target organs. AEX chromatography also allowed to observe the formation of complexes between Hg<sup>2+</sup> and MeHg<sup>+</sup> with L-cysteine at pH 7.4, that are implicated in their organ uptake. Lastly, the application of reversed phase (RP) chromatography revealed a possible cytosolic mechanism by which N-acetyl-L-cysteine binds to MeHg<sup>+</sup> in the presence of cytosolic glutathione (GSH). New insight into other bioinorganic processes may advance the regulatory framework to better protect public health.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0