Visnyk of Dnipropetrovsk University. Biology, medicine最新文献

{"title":"The influence of external factors on the accuracy of non-invasive measuring of oxygen in blood","authors":"Y. M. Snizhko, V. Sarana","doi":"10.15421/021614","DOIUrl":"https://doi.org/10.15421/021614","url":null,"abstract":"In this paper we investigated a pulse oximetry-based method for mobile devices. This method obtains bio-signals related to blood pulsation in transparent parts of body. The most widely accepted field for use of this method is hospital care. In these cases a pulse oximeter is the best solution for the monitoring of emergency patients. A promising field for pulse oximetry is physical exercise. It only requires simple clips such as ear-clips, finger-clips, headbands etc. However this method presents some difficulties: weak signal, noise ratio, motion artefacts, low perfusion. We used a MAX30100 Oximeter and Heart Rate Sensor integrated circuit to obtain signals of blood pulse waves from red and infrared light emission diodes (LED). This device measures the oxygen saturation of a person’s blood by placing an LED and a photodetector against the thin skin of a person’s body, such as a fingertip, wrist or earlobe. The MAX30100 is a 14-pin surface mount integrated circuit that contains sensors for measuring a person’s heart rate. It can also indirectly determine the oxygen saturation of a person’s blood. The MAX30100 provides a complete pulse oximetry and heart rate measurement solution for medical monitors and wearable fitness devices. As each LED emits light into a person’s finger, the integrated photodetector measures variations in light caused by changes in blood volume. An integrated 16-bit analog to digital converter (ADC) with programmable sample rate converts the photodetector output to a digital value. The MAX30100 filters out ambient light that can interfere with an accurate reading. Data are read through a serial I2C interface to computer for further processing. The LED current can be programmed from 0 to 50 mA with proper supply voltage. The LED pulse width can be programmed from 200 µs to 1.6 ms to optimize measurement accuracy and power consumption based on use cases. The SpO 2 algorithm is relatively insensitive to the wavelength of the infrared LED, but the red LED’s wavelength is critical to correct interpretation of the data. The temperature sensor data can be used to compensate errors with ambient temperature changes. During the experiments we registered oxygen concentration values under different conditions. With low muscular activity we observed high stability and repeatability of measuring values under various exterior conditions. However, with high muscular activity there were various artefacts in the gauged signals that led to contortion of effects. We identified the boundaries of the validity of measuring and propose the use of an adapted filter in order to distinguish pulse waves from optical signals more reliably. These devices can be applied in fitness training , medical monitoring and used as wearable devices.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"190 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132105257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of moderate physical load on parameters of the immune system among residents of contaminated areas","authors":"V. Sokolenko, S. Sokolenko","doi":"10.15421/021609","DOIUrl":"https://doi.org/10.15421/021609","url":null,"abstract":"The aim of this research was to evaluate the effects of physical stress caused by physical activity on parameters of immune system among the residents of areas contaminated with radionuclides. In the 2000–2015 we examined 125 students ofCherkasyStateUniversity, including the control group of people from uncontaminated areas, persons working in a basic physical training group and those with symptoms of vegetative-vascular dystonia, who worked in a therapeutic physical training group. Immune system parameters were analyzed: a day before physical training, immediately after the training and two days after the training to assess the recovery period. Indicators of cellular immunity were determined by immunophenotyping and dyeing on Romanowsky-Giemsa. The level of immunoglobulins in blood serum was determined by radial immunodiffusion on Mancini. The level of cortisol in blood serum was determined by the immunoenzyme method. Here we established that even in the absence of physical activity, some immunosuppression of T-cell immunity was observed in residents of contaminated areas. Working in the basic physical training group resulted in a significant decrease in the relative number of lymphocytes and increasing in the relative number of band neutrophils, which is a typical feature of the early stages of stress response. A statistically significant reduction in relative and absolute number of cells with phenotypes CD3+, CD5+, CD4+ and immunoregulatory index CD4+/CD8+ was observed. There were no significant changes of cytotoxic T lymphocytes with phenotype CD8+ and natural killer cells with phenotype CD16+. Increase of the relative number of B cells, that express CD72 antigen, and growth trend in serum IgM were registered. All parameters analyzed were within the physiological homeostatic norm, however, some reached extreme recommended levels. Recovery period lasted 2 days. Individuals working in therapeutic physical training group did not show statistically significant changes in immune system parameters. Thus, therapeutic exercises don't reach the stress level and can be potentially safe for the natural resistance of the body. So, among residents of areas contaminated with radionuclides due to the Chernobyl accident, moderate load during physical training lessons causes short-term compensatory changes of cellular immunity within the homeostatic norm with effective and rapid recovery. Taking into account the immunosuppression, caused by chronic exposure to low doses of ionizing radiation, it is important to choose exercises, their duration and intensity carefully , giving preference to therapeutic exercises.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128287789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioelectrical activity of the brain during performance of manipulative movements in women with different modal alpha-frequencies","authors":"A. Morenko","doi":"10.15421/021612","DOIUrl":"https://doi.org/10.15421/021612","url":null,"abstract":"An important component of the general biological problem of coordinated activity of the brain’s motor systems and of the executive apparatus during the implementation of motor functions is the question of the interconnection of electroencephalogram (EEG) rhythm characteristics, above all α-rhythm, with control peculiarities of manipulative movements (MM). A test group consisting of 113 healthy right-handed women from the ages of 19 to 21 was divided into two groups according to the average magnitude of their individual modal α-frequency – groups with high and low values of individual modal α-frequency (IαF). The ideal time of a simple sensorimotor reaction and choice-point behavior as well as speed capabilities of nervous processes during the finger tapping test, and measures of the power spectrum of ЕЕG spectral components individually determined for each testee in quiescent intervals and while performing alternating movements by the fingers of the right hand were evaluated. Alternate female finger movements were accompanied by a decrease in the EEG α2- and α3-activities in the posterior cortical areas, and β1- and γ-activities in the frontal, temporal and central areas of the cortex compared with the same in the immobile state; they were also characterized by the generalized growth of θ-oscillations and local (in the frontal leads) – α1- and β2-activities. Thus, in both groups of women surveyed the increase in activity of those cortical structures that ensure its implementation – sensory perception, motor start and motor programming commands and sense-motor coordination, was found. When MM are performed by women with a higher output α-frequency, they were characterized by more local changes in the electrical activity of the cerebral cortex. People with a lower modal α-frequency were characterized by somewhat less specific and differentiated features of the cortex activation. Alternating movements of fingers performed by women with low IαF were associated with higher EEG θ-, α1-, α2-activities, generally in the cortex, and α3-, β- and γ-oscillations – in the posterior areas than in those with high IαF. Instead of this, a relatively lower power spectral EEG was recorded in the frontal leads of the range consisting of α3-, β- and γ-oscillations. Intergroup differences found by us may indicate a higher status regarding a specific cortical tone, its readiness for activity and control of information processes in people with high IαF in comparison with those who had low IαF. It is natural that different levels of features of such activation systems achieve specific outcomes in testees with a different starting IαF. Women with a higher IαF had better speed characteristics of nervous processes.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117102211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and cellular mechanisms of profile changes of charged blood plasma free oligosaccharides in myeloproliferative disorders","authors":"I. U. Pismenetskaya, T. Butters","doi":"10.15421/021611","DOIUrl":"https://doi.org/10.15421/021611","url":null,"abstract":"Free oligosaccharides (FOS) are unbound to proteins or lipids structural analogs of their glycans. FOS appear as by-products of endoplasmic reticulum synthesis, cell control folding with endoplastic reticulum-associated degradation and lysosomal/endosomal breakdown of glycoconjugates. They may be either neutral or negatively charged depending on the way of their formation. Charged FOS appear during degradation of glycoconjugates in the lysosomal/endosomal system and are natural substrates for lysosomal sialidase-1. FOS are formed inside the cell but some of them can get into the extracellular space, and then into the blood and urine, where both neutral and charged structures were found. Secretion of charged FOS outside of the cell is most likely to be caused by the lysosomal exocytosis. The activity of neuraminidase-1 is known to be connected with the intensity of the lysosomal exocytosis. In our previous studies, it was found that HPLC-profiles of charged FOS were specifically changed in acute and chronic myeloproliferative blood disorders. The objective of this work was to analyze the molecular and cellular mechanisms of these changes and to test the hypothesis of their association with the activity change of lysosomal neuraminidase-1. Plasma samples of patients with acute and chronic leukaemia – polycytaemia vera, chronic idiopathic myelofibrosis, hypoplastic anaemia, myelodysplastic syndrome with transformation, acute lymphatic and acute myelomonocytic leukaemia – were collected for investigation. Plasma samples of practically healthy volunteers were obtained and used for comparison. After plasma deproteinization and FOS purification the oligosaccharides were labelled with anthranilic acid (2-AA), separated into the neutral and charged fractions with QAE Sephadex (Q25-120) chromatography and analysed using high-performance liquid chromatography (HPLC). Glucose unit values were determined following comparison with a 2-AA-labelled glucose oligomer ladder derived from a partial hydrolysate of dextran as an external standard. The data were collected and processed using Empower software. The charged FOS were digested with the sialidase from Arthrobacter ureafaciens . 2-AA – labelled free oligosaccharides from transferrin were used as an external standard for the structure decoding. In this paper, the changes in neraminidase-1 activity was first demonstrated on natural substrates, free oligosaccharides, in vivo . Chromatographic profiles of charged plasma FOS of patients with acute and chronic hematological malignancies revealed decreasing of neuraminidase-1 activity and increasing of the lysosomal exocytosis. Thus, chromatographic profiles of charged plasma FOS appeared to be a sensitive parameter of the lysosomal/endosomal status in normal or pathological states and to open up new prospects for their applications to the development of anti-cancer drugs designed to block the work of the lysosomal/endosomal system and monitoring of their action.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132986072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of the supporting lower limb function in the premotor changes in the H-reflex of m. soleus","authors":"E. Ivanchenko","doi":"10.15421/021608","DOIUrl":"https://doi.org/10.15421/021608","url":null,"abstract":"In studies on healthy humans we examined changes in the H reflex amplitude and the intensity of background EMG of the soleus muscle within the premotor period of voluntary extension of the contralateral ankle joint. The tested persons were in the lying prone position with relaxed legs, or in the standing position with support on both feet or only on one foot with additional hand support. The soleus H reflex was induced using the standard technique by transcutaneous stimulation (1 ms long current pulses) of the tibial nerve afferents in the region of the popliteal dimple. Using superficial electrodes, we recorded the integral EMG reflex discharge from the m. soleus , amplified, and visualized it using a two-channel digital oscillograph, Handiscope HS3 (TiePieEngeneering, Netherlands). In other tests, we also recorded tonic background EMG activity from the soleus muscle. After fullwave rectification and low-frequency filtration we estimated the area under the integral curve enveloping oscillations of the above mentioned EMG. In the lying position the extension of contralateral ankle joint evoked anticipatory facilitation of the testing soleus H-reflex. It manifested in 90–60 ms and reached the maximum 30 ms before the conditioning movement. Considerable changes of the soleus background EMG-activity did not occur during the premotor period. Consequently, it is supposed that the mechanism leading to the H-reflex facilitation is the decrease of presynaptic inhibition at 1a. In the standing position with support on both feet contralateral ankle joint extension caused anticipatory inhibition of the soleus H-reflex and simultaneous suppression of background EMG 90–60 ms before the conditioning movement. These results indicate the anticipatory changes of muscle tone and suggest participation of postsynaptic mechanism in the inhibition of the testing H-reflex. When the moving contralateral extremity had no support, the contralateral ancle joint extension did not cause anticipatory inhibition but facilitation of the soleus H-reflex. Its time course was similar to that observed in the lying position. The considerable changes of the soleus background EMG-activity did not arise. The results of the experiments indicate that the nature of premotor changes in the spinal neuron circuits, associated with the lower limb muscles, depends not only on the type of upcoming voluntary movement. Being caused by the contralateral limb movements, they depend greatly on the fulfillment or nonfulfillment of the support function by the lower limbs.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130804595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological parallels of communications between the circumventricular system in adolescents","authors":"T. Komshuk","doi":"10.15421/021607","DOIUrl":"https://doi.org/10.15421/021607","url":null,"abstract":"This article presents, based on the example of morphometric study of magnetic resonance tomograms of persons of various ages, the complex of vital characteristics of the cerebrospinal system of the brain in adolescents. The study tested gender characteristics and inter hemispheric asymmetry of the analyzed indexes. An asymmetry of anatomical structures of the right and left hemispheres with the tendency to increase in their sizes in the right hemisphere was found both for males and for females. Females had some age differences in the longitudinal dimension of the III ventricle (this index was lower in older females), the width and index of the IV ventricle was significantly lower in the 18 and 20 year old girls. Objective and methods. The research was conducted on the base of the radiation diagnosis department of Rivne Regional Clinical Hospital on the CT scan General Electric Healthcare Signa MRI 1.5 T and in the office of magnetic resonance imaging of Lutsk City Clinical Hospital on the CT scan Signa Profile Ce Medical System – 1,5 Tl in standard anatomic planes (sagittal, frontal andaxial). Measurements were carried out in persons without visual signs of organic lesions of the brain and skull. Analysis was made of 11 tomograms (males 17–21 years old – 6 persons, females 16–20 years old – 5 persons). In comparing pairs of parameters (of lateral ventricles) the factor of asymmetry was calculated, which is equal to the difference between the right and left lateral ventricles divided by the sum of right and left ventricles indexes (%). The results were processed by methods of descriptional statistics. In young males of this age group a significant increase in the following indicators was identified: the length of the anterior horn of the lateral ventricle on the right side, the length of the body of the lateral ventricle on both sides and the height of the III ventricle. Females had significantly increases in the following parameters: the width of the anterior horn of the lateral ventricle on the right side and the width of the posterior horn of the lateral ventricle on the left side. Consequently, in male adoles cents a parallelism in the formation of various structures of the ventricular system was traced. Since this is a single system, morphological and functional connections between its different components are stored. Mathematical analysis of the values of the structures of ventricular system in females characterize a hormonal influence on its development in adolescents. We can assume that organs which are the part of the circumventricular system (subcommisural organ, subfornical organ, median rise etc.), definitely have the character of anatomical connections between these components. Their functional unity in maintaining the homeostasis of the body is undeniable.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125441200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of citrate and sulfate of different metals on carbohydrates composition of medicinal mushroom Trametes versicolor (Polyporales, Polyporaceae)","authors":"G. Al-Maali, N. Bisko, A. Ostapchuk","doi":"10.15421/021606","DOIUrl":"https://doi.org/10.15421/021606","url":null,"abstract":"The aim of our research was to study the influence of citrate and sulfate of copper, manganese and zinc on the carbohydrates composition of biomass of the medicinal mushroom Trametes versicolor (L.) Lloyd. cultivated in a liquid medium. The studied strain of Trametes versicolor 353 was obtained from the Culture Collection of Mushrooms (IBK) from the M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine. Citrates of copper, manganese and zinc were obtained from the Institute of Nanobiotechnologies and Resource Conservation of Ukraine, Kyiv. In this study we used glucose-peptone-yeast extract medium (GPY) with the following composition of (g/L): glucose – 25, peptone – 3, yeast extract – 3, K 2 HPO 4 – 1, KH 2 PO 4 – 1, MgSO 4 ∙ 7H 2 O – 0.25, distilled water – 1,000 ml; pH 6,5 (control medium). Cu 2+ (sulfate form or citrate form) were added to control medium in concentration 4 mg/L, Zn 2+ and Mn 2+ (sulfate form or citrate form) were added to control medium in concentration 1 mg/L. Mycelium was grown in a submerged culture on a rotary shaker (120 rpm) at 26 °C in 250 ml Erlenmeyer flasks, containing 50 ml of liquid medium. The biomass was harvested after 9 days of cultivation in the liquid medium. Monosaccharides and sugar alcohols was analyzed by high-performance liquid chromatography Agilent 1200 (Agilent technologies, USA). The results of our research demonstrated that the fraction of total carbohydrates from T. versicolor 353, which were cultivated on the control medium, consists of glucose (91.5%), galactose (1.9%), mannose (5.4%), fucose (0.6%) and xylose (0.7%). The adding of sulfate of zinc or copper to the liquid medium didn’t change the content of monosaccharides of T. versicolor 353 biomass relative to the control medium. At the same time, in the medium with citrate of zinc the amount of glucose increased and the amount of mannose decreased relative to the control medium. Xylose and fucose were not detected in this case. In mycelium of T. versicolor 353 that was cultivated in the medium with manganese sulfate or manganese citrate we detected a small amount of ribose. But the content of other monosaccharides didn’t change in the medium with sulfate of manganese. At the same time, the addition of citrate of manganese to the medium raised the amount of mannose and reduced the amount of glucose in the biomass of T. versicolor 353 relative to the control medium. Also copper citrate reduced the content of glucose in mycelium of T. versicolor 353 relative to the control medium. But in this case we detected mannitol and sorbitol in the biomass. This data indicates that synthesis of sugur alcohols in T. versicolor 353 depend on copper ions in bioactive forms of citrate.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115131743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of efficiency of using the enzyme preparation «Celloviridin Dx20» in the content of pig feed","authors":"A. O. Dejnega, V. O. Lesova, A. Anatsky","doi":"10.15421/021603","DOIUrl":"https://doi.org/10.15421/021603","url":null,"abstract":"One of the main problems in the feeding of farm animals is the low degree of digestion and assimilation of nutrients in the feed used in animal production. In the bodies of animals such as pigs and poultry enzymes and microflora which are necessary for breaking down cellulose and other polysaccharides of vegetable raw materials are absent, therefore they not only fail to be digested in the gastrointestinal tract, but also obstruct access of other digestive enzymes to the other feed components, in particular intracellular proteins. The only way to solve this problem is deliberate introduction of enzymes into food. Depending on the type and composition of the feed, amylolytic, proteolytic, cellulolytic enzyme preparations are used. The purpose of this work is to assess the effect of the enzyme preparation \"Celloviridin Dx20\" as a fodder component on the growth and biochemical parameters of young pigs. For this purpose, a scientific and economic experiment on feeding young pigs was conducted at the agro-industrial farm«Niva» (Novonikolaevka village, Dnipropetrovsk region), in which the enzyme preparation «Celloviridin Dx20», which promotes the breakdown of plant cell polysaccharides (cellulose, xylans) was introduced to a standard feed mixture used in swine husbandry. The composition of the ration consisted of 60% barley, 20% wheat and 20% of the protein-vitamin-mineral complex «ShenPigStart». The results of the experiment showed that the introduction into the feed mixture of the enzyme preparation at 100 g per ton helps to increase the body weight of pigs by 11.6% compared to the control, can increase the digestion rate of fibre (13.8%), protein and fat. These results can be explained by the mechanism of action of the enzyme complex, which consists in the hydrolysis of polysaccharides of plant cell walls in the feed in the digestive tract of pigs, the formation of digestable polysaccharides and the release of additional quantities of intracellular proteins. The result of these phenomena is also a positive trend in feed nitrogen assimilation in animals of the experimental group: a 19% decrease in the amount of physiological secretions and a 12.5% increase in nitrogen use. At the same time hematology (hemoglobin, erythrocytes, leukocytes), calcium and phosphorus metabolism remained unchanged. This shows that the preparation used does not accumulate in the body and gradually loses enzymatic activity, and under the influence of the pigs’ stomach proteases it is broken down into separate amino acids which are absorbed by the animals. The resulting stimulatory effect of using \"Celloviridin Dх20\" has not only physiological but also economic importance, as it helps reduce the time for the pigs to reach slaughter weight (close to 100 kg) by 11 days, and consequently, reduces feed intake and its costs in rearing pigs on farms. Therefore, it is advisable to use \"Celloviridin Dх20\" in swine husbandry.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124854776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological charactheristic of catheterized urinary tract as flow system","authors":"V. P. Zhalko-Tytarenko, E. A. Synetar, S. I. Savoschenko","doi":"10.15421/021610","DOIUrl":"https://doi.org/10.15421/021610","url":null,"abstract":"It is well known that the ureter of mammals and man works as a flow system that normally remains uninfected. In biotechnology flow systems are used to produce microbial mass on orders greater than when grown in laboratory and industrial periodic cultures. Therefore, these systems are used in the microbiological industry. But there ina flow systems one can observe a growth in microbial populations, the patterns of which can be fruitful in studying the process of catheter-associated urinary tract infections. The main factors are the speed of propagation and speed of flow, and their variation in conditions of the catheterized urinary tract, which determines the magnification factor of microbial infection. In this lies the general biological need to study them from a biotechnology perspective. Hence the question arises – why are there changes in the flow system of the urinary tract during catheterization, which contribute to the reproduction of opportunistic microorganisms, as a factor of infection? In the study of the biological properties of agents of catheter-associated infections this issue has not attracted sufficient attention from researchers. In view of the above, the purpose of our study was to establish patterns of population growth of agents of urinary tract infections on the model of the yeast fungus species Candida albicans (C.P. Robin) Berkhout, 1923 in the catheterized human urinary tract, treated as a flow system. The results of the research show that the emergence of catheter-associated urinary tract infections is a regularity inherent throughout the flow system. It was proved that with the model C. albicans , bacteria in urine multiply faster than in the medium Saburo. The study established that one of the factors leading to catheter-associated urinary tract infections is the persistent presence of urine deposits around the head of the catheter in the bladder, which provides bacteria with sufficient conditions for reproduction and where bacteria cannot be eliminated by urine flow. When the pathogen enters and reproduces in the bladder, the latter is a reservoir of infection like a \"royal culture\", with a gradual overgrowth in the catheter of yeast fungi of the species C. albicans . These data suggest that the mechanism of strengthening catheter-associated urinary tract infections are the formation of biofilms by microorganisms around the catheter head and on its surfaces. It is recommended to further improve the design of urinal catheters in such a way that ensures the complete emptying of the bladder and avoidance of urethral reflux.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"184 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122631037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of oxidative stress on the state of the antioxidant defense system in the organism of rats","authors":"T. Martyshuk","doi":"10.15421/021602","DOIUrl":"https://doi.org/10.15421/021602","url":null,"abstract":"Наведено результати досліджень впливу оксидаційного стресу на інтенсивність процесів перекисного окиснення ліпідів та активність глутатіонової системи антиоксидантного захисту організму щурів. Внутрішньом’язове уведення щурам дослідної групи 50% розчину тетрахлорметану у дозі 0,25 мл на 100 г маси тіла тварини спричиняє активацію процесів вiльнорадикального окислення з надмірним накопиченням проміжних та кінцевих продуктів перекисного окиснення ліпідів. Результати досліджень вказують на те, що розвиток оксидаційного стресу викликає значне та вірогідне прискорення утворення та накопичення в плазмі крові щурів у всі терміни дослідження гідроперекисів ліпідів і малонового діальдегіду. Найвищим рівень гідроперекисів ліпідів у плазмі крові щурів за оксидаційного стресу був на другу добу досліду (0,843 од. Е/мл) порівняно з контролем (0,245 од. Е/мл). Під час дослідження вмісту малонового діальдегіду встановлено, що він був вищим у 2,03 раза порівняно з контролем у тварин дослідної групи на п ’ яту добу. На 10- та 14-ту добу досліду відмічаємо незначне зниження рівня гідроперекисів ліпідів і малонового діальдегіду. Розвиток оксидаційного стресу також спричинює пригнічення активності глутатіонової системи антиоксидантного захисту організму щурів. Про це свідчить низька активність глутатіонпероксидази та низький рівень відновленого глутатіону у крові щурів дослідної групи. На п’яту добу досліду активність глутатіонпероксидази та рівень відновленого глутатіону у крові щурів, яким уводили тетрахлорметан, був найнижчим (порівняно з контролем дані показники знизилися відповідно на 53% і 51%). На 10-ту та 14-ту добу досліджень активність глутатіонпероксидази та рівень відновленого глутатіону у крові щурів дослідної групи дещо зросли, однак порівняно із контролем були вірогідно нижчими. Встановлено суттєве порушення окисно-антиоксидантної рівноваги у тварин за оксидаційного стресу, яке характеризується активацією процесів вiльнорадикального окиснення ліпідів із надмірним накопиченням проміжних та кінцевих продуктів ПОЛ, пригніченням активності системи антиоксидантного захисту.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116945972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}