IF 2.7

BioTech Pub Date : 2025-05-08 DOI: 10.3390/biotech14020035

Gleb Aktuganov, Alexander Lobov, Nailya Galimzianova, Elena Gilvanova, Lyudmila Kuzmina, Polina Milman, Alena Ryabova, Alexander Melentiev, Sergey Chetverikov, Sergey Starikov, Sergey Lopatin

{"title":"Comparative Potential of Chitinase and Chitosanase from the Strain Bacillus thuringiensis B-387 for the Production of Antifungal Chitosan Oligomers.","authors":"Gleb Aktuganov, Alexander Lobov, Nailya Galimzianova, Elena Gilvanova, Lyudmila Kuzmina, Polina Milman, Alena Ryabova, Alexander Melentiev, Sergey Chetverikov, Sergey Starikov, Sergey Lopatin","doi":"10.3390/biotech14020035","DOIUrl":"10.3390/biotech14020035","url":null,"abstract":"The depolymerization of chitosan using chitinolytic enzymes is one of the most promising approaches for the production of bioactive soluble chitooligosaccharides (COS) due to its high specificity, environmental safety, mild reaction conditions, and potential for development. However, the comparative efficacy of bacterial chitinases and chitosanases in terms of yield, solubility, and antimicrobial activity of produced COS remains understudied. In this work, chitinase (73 kDa) and chitosanase (40 kDa) from the strain Bacillus thuringiensis B-387 (Bt-387) were purified using various chromatographic techniques and compared by their action on chitosan (DD 85%). The molecular mass and structure of generated COS was determined using TLC, LC-ESI-MS, HP-SEC, and C13-NMR techniques. Chitosanase converted the polymer more rapidly to short COS (GlcN2-GlcN4), than chitinase, and was more specific in its action on mixed bonds between GlcN and GlcNAc. Chitosanase needed a noticeably shorter incubation time and enzyme-substrate ratio than chitinase for production of larger oligomeric molecules (Mw 2.4-66.5 and 15.4-77.7 kDa, respectively) during controlled depolymerization of chitosan. Moreover, chitosanase-generated oligomers demonstrate better solubility and a higher antifungal activity in vitro against the tested plant pathogenic fungi. These features, as well as the high enzyme production and its simplified purification protocol, make chitosanase B-387 more suitable for the production of antifungal chitooligomers than chitinase.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Effect of Spent Mushroom Substrate and Agro-Industrial Residues on Pleurotus columbinus Production and Intra-Cellular Polysaccharide Synthesis. 食用菌废底物与农工残留物对侧耳生产及胞内多糖合成的联合影响

IF 2.7

BioTech Pub Date : 2025-05-02 DOI: 10.3390/biotech14020034

Marianna Dedousi, Chrysavgi Gardeli, Seraphim Papanikolaou, Panagiota Diamantopoulou

{"title":"Combined Effect of Spent Mushroom Substrate and Agro-Industrial Residues on Pleurotus columbinus Production and Intra-Cellular Polysaccharide Synthesis.","authors":"Marianna Dedousi, Chrysavgi Gardeli, Seraphim Papanikolaou, Panagiota Diamantopoulou","doi":"10.3390/biotech14020034","DOIUrl":"10.3390/biotech14020034","url":null,"abstract":"Spent mushroom substrate (SMS), spent coffee grounds from espresso production (SCG), faba bean harvest residues (FBR), pistachio shells (PS) wheat straw (WS) (control) agro-industrial waste were combined in different ratios, with or without supplements (wheat bran, soybean flour), to create novel substrates for Pleurotus columbinus growth. The impact of the substrates on the mycelial growth rate (Kr), biomass production, laccase, total cellulases and carbohydrate synthesis, along with the C and N consumption by P. columbinus, were examined in fully colonized substrates. The incubation period, earliness and biological efficiency (B.E.) (%) were also determined. Then, the intracellular polysaccharide (ICP) contents of the P. columbinus produced mushrooms were evaluated in the most promising substrates. P. columbinus was grown successfully in a wide range of C/N ratios of substrates and the fastest Kr (7.6 mm/d) was detected on the 70 SMS-30 FBR, without supplements, whereas substrates consisting of SCG enhanced biomass production (700.0-803.7 mg/g d.w.). SMS and PS or SCG led to the shortest incubation and earliness period of P. columbinus. The C content was reduced and the N content was substantially increased in all the colonized substrates. The 70 SMS-30 FBR and 80 SMS considerably enhanced the laccase production (up to 59,933.4 U/g d.w.) and substrates consisting of PS promoted total cellulases activities. Greater amounts of carbohydrates (3.8-17.4 mg/g d.w.) than that in the control were recorded for all the substrates. The combination of SMS and SCG or WS led to the highest B.E. values (59.3-87.1%) and ICP amounts (34.7-45.9%, w/w), regardless of the supplement addition. These findings support the effective utilization of agro-industrial waste in P. columbinus cultivation, producing high-value-added compounds and supporting mushroom growth.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovations in Proteomic Technologies and Artificial Neural Networks: Unlocking Milk Origin Identification. 蛋白质组学技术和人工神经网络的创新：解锁牛奶来源鉴定。

IF 2.7

BioTech Pub Date : 2025-04-28 DOI: 10.3390/biotech14020033

Achilleas Karamoutsios, Emmanouil D Oikonomou, Chrysoula Chrysa Voidarou, Lampros Hatzizisis, Konstantina Fotou, Konstantina Nikolaou, Evangelia Gouva, Evangelia Gkiza, Nikolaos Giannakeas, Ioannis Skoufos, Athina Tzora

{"title":"Innovations in Proteomic Technologies and Artificial Neural Networks: Unlocking Milk Origin Identification.","authors":"Achilleas Karamoutsios, Emmanouil D Oikonomou, Chrysoula Chrysa Voidarou, Lampros Hatzizisis, Konstantina Fotou, Konstantina Nikolaou, Evangelia Gouva, Evangelia Gkiza, Nikolaos Giannakeas, Ioannis Skoufos, Athina Tzora","doi":"10.3390/biotech14020033","DOIUrl":"10.3390/biotech14020033","url":null,"abstract":"Milk's biological origin determination, including its adulteration and authenticity, presents serious limitations, highlighting the need for innovative advanced solutions. The utilisation of proteomic technologies combined with personalised algorithms creates great potential for a more comprehensive approach to analysing milk samples effectively. The current study presents an innovative approach utilising proteomics and neural networks to classify and distinguish bovine, ovine and caprine milk samples by employing advanced machine learning techniques; we developed a precise and reliable model capable of distinguishing the unique mass spectral signatures associated with each species. Our dataset includes a diverse range of mass spectra collected from milk samples after MALDI-TOF MS (Matrix-assisted laser desorption/ionization-time of flight mass spectrometry) analysis, which were used to train, validate, and test the neural network model. The results indicate a high level of accuracy in species identification, underscoring the model's potential applications in dairy product authentication, quality assurance, and food safety. The current research offers a significant contribution to agricultural science, providing a cutting-edge method for species-specific classification through mass spectrometry. The dataset comprises 648, 1554, and 2392 spectra, represented by 16,018, 38,394, and 55,055 eight-dimensional vectors from bovine, caprine, and ovine milk, respectively.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Multifunctional Hydrolysates from the Lupinus mutabilis Protein Using a Micrococcus sp. PC7 Protease. 利用微球菌PC7蛋白酶从变异狼疮蛋白生产多功能水解物。

IF 2.7

BioTech Pub Date : 2025-04-27 DOI: 10.3390/biotech14020032

Keyla Sofía Llontop-Bernabé, Arturo Intiquilla, Carlos Ramirez-Veliz, Marco Santos, Karim Jiménez-Aliaga, Amparo Iris Zavaleta, Samuel Paterson, Blanca Hernández-Ledesma

{"title":"Production of Multifunctional Hydrolysates from the Lupinus mutabilis Protein Using a Micrococcus sp. PC7 Protease.","authors":"Keyla Sofía Llontop-Bernabé, Arturo Intiquilla, Carlos Ramirez-Veliz, Marco Santos, Karim Jiménez-Aliaga, Amparo Iris Zavaleta, Samuel Paterson, Blanca Hernández-Ledesma","doi":"10.3390/biotech14020032","DOIUrl":"10.3390/biotech14020032","url":null,"abstract":"The growing demand for functional foods has driven the search for bioactive compounds derived from plant proteins. Lupinus mutabilis \"Tarwi\", a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bioactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functionally classified into three groups: cellular component (35.57%), molecular function (33.45%), and biological process (30.97%). The isolated AF was hydrolysed with the native protease PC7 (HAP), optimizing the E/S ratio and time parameters. Additionally, sequential hydrolysis of the PC7 protease and alcalase (HAPA) was performed. In vitro multifunctionality assays, HAP and HAPA demonstrated the ability to scavenge radicals (ABTS and ORAC) and inhibit angiotensin-converting enzyme (ACE)-I and dipeptidyl peptidase IV (DPP-IV). The findings of this study highlight the potential of L. mutabilis albumin hydrolysate as a multifunctional ingredient for functional foods aimed at managing chronic conditions associated with oxidative stress, hypertension, and/or metabolic disorders.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaginal Microbiota Patterns Associated with Yeast Infection in Mexican Women, a Pilot Study. 墨西哥妇女阴道微生物群模式与酵母菌感染相关，一项初步研究。

IF 2.7

BioTech Pub Date : 2025-04-26 DOI: 10.3390/biotech14020031

Janet Pineda-Díaz, Carolina Miranda-Brito, Carmen Josefina Juárez-Castelán, Alberto Piña-Escobedo, Noemí Del Socorro Lázaro-Pérez, Alejandra de la Cruz-Munguía, Daniela Ramírez-Sánchez, Yuliana Gómez-Meraz, Juan Manuel Vélez-Ixta, Jaime García-Mena

{"title":"Vaginal Microbiota Patterns Associated with Yeast Infection in Mexican Women, a Pilot Study.","authors":"Janet Pineda-Díaz, Carolina Miranda-Brito, Carmen Josefina Juárez-Castelán, Alberto Piña-Escobedo, Noemí Del Socorro Lázaro-Pérez, Alejandra de la Cruz-Munguía, Daniela Ramírez-Sánchez, Yuliana Gómez-Meraz, Juan Manuel Vélez-Ixta, Jaime García-Mena","doi":"10.3390/biotech14020031","DOIUrl":"10.3390/biotech14020031","url":null,"abstract":"Recurrent vulvovaginal candidiasis (RVVC) is a common condition that affects women of reproductive age. The etiology of RVVC remains largely unknown, but it is believed to be associated with changes in vaginal microbiota composition. This study investigates the vaginal microbiota in 57 women with RVVC and 38 healthy controls. Bacterial DNA was analyzed using high-throughput 16S rRNA gene sequencing, and Candida and Saccharomyces species were determined by PCR. RVVC cases had a higher prevalence of Nakaseomyses glabratus (former Candida glabrata) compared to controls. Alpha diversity metrics were similar between groups, but beta diversity analysis revealed significant differences in vaginal microbiota composition. The Firmicutes abundance was altered in RVVC cases, with genus Bifidobacterium and phylum Actinobacteriota being more abundant than in the controls. At the genus level, Lactobacillus dominated controls using antibiotics, while Bifidobacterium was higher in cases with no antibiotic intake. Our study provides evidence that Nakaseomyses glabratus (former Candida glabrata) is a significant pathogen in RVVC, while Candida albicans was more prevalent in healthy women. The vaginal microbiota composition differs significantly between the two groups, with distinct patterns of bacterial abundance and changes in Firmicutes abundance.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Biotechnology of Three New Strains of Basidial Fungi as Promising Sources of Biologically Active Substances. 三种具有生物活性物质的担子真菌新菌株的鉴定与生物技术研究。

IF 2.7

BioTech Pub Date : 2025-04-25 DOI: 10.3390/biotech14020030

Maria Alexandrovna Sysoeva, Ilyuza Shamilevna Prozorova, Elena Vladislavovna Sysoeva, Tatyana Vladimirovna Grigoryeva, Ruzilya Kamilevna Ismagilova

{"title":"Characterization and Biotechnology of Three New Strains of Basidial Fungi as Promising Sources of Biologically Active Substances.","authors":"Maria Alexandrovna Sysoeva, Ilyuza Shamilevna Prozorova, Elena Vladislavovna Sysoeva, Tatyana Vladimirovna Grigoryeva, Ruzilya Kamilevna Ismagilova","doi":"10.3390/biotech14020030","DOIUrl":"10.3390/biotech14020030","url":null,"abstract":"The study of new strains of basidiomycetes as sources of biologically active substances is a promising direction in modern biotechnology. This work aims to isolate new cultures of the fungi Daedaleopsis tricolor, Pycnoporellus fulgens and Trichaptum abietinum from natural fruiting bodies and to improve their growth conditions on solid nutrient media. The identification of fungi was performed based on their morphological features and using the Sanger sequencing method. Cultivation was carried out by placing inoculum in the middle of a Petri dish and at the edge, which provided a more comprehensive definition of the characteristics of colonies and fungus hyphae. New strains were registered in Genbank Overview. The optimal cultivation temperature was 27 °C for all studied strains. The highest radial growth was observed on synthetic medium for D. tricolor (5.26 mm/day) and T. abietinum (7.5 mm/day), and on synthetic medium with lignin for P. fulgens (2.98 mm/day). The biomass amount of D. tricolor KS11 was 133.25 mg at 9 days of cultivation, that of P. fulgens KS12 was 86.73 mg at 16 days, and that of T. abietinum KS10 was 227.33 mg at 6 days. New strains of fungi can be used to obtain biologically active substances for the food, pharmaceutical and cosmetic industries.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct Shoot Regeneration from the Finger Millet's In Vitro-Derived Shoot Apex and Genetic Fidelity Study with ISSR Markers. 五味子离体茎尖直接再生及ISSR标记遗传保真度研究。

IF 2.7

BioTech Pub Date : 2025-04-18 DOI: 10.3390/biotech14020029

Theivanayagam Maharajan, Veeramuthu Duraipandiyan, Thumadath Palayullaparambil Ajeesh Krishna

{"title":"Direct Shoot Regeneration from the Finger Millet's In Vitro-Derived Shoot Apex and Genetic Fidelity Study with ISSR Markers.","authors":"Theivanayagam Maharajan, Veeramuthu Duraipandiyan, Thumadath Palayullaparambil Ajeesh Krishna","doi":"10.3390/biotech14020029","DOIUrl":"https://doi.org/10.3390/biotech14020029","url":null,"abstract":"Globally, people are cultivating finger millet, an important cereal, to improve food availability and health benefits for humans. However, the biotechnological research on this millet is limited and insufficient in this field. The primary focus of this study is to optimize an efficient regenerated protocol for initiating further plant transformation studies, using the shoot apex as an explant and various growth regulators. For example, three cytokinins (BAP, TDZ, and Kin) at different concentrations were used to induce multiple shoots of finger millet. Among these, TDZ (4.5 µM) provided the maximum number (17.3) of shoots as compared to BAP and Kin. IBA (2.46 µM), along with MS medium, was used for the induction of roots, where 5.6 roots were produced in an individual shoot and the length of the root was longer with a size of 8.2 cm after two weeks of incubation. The clonal fidelity of the in vitro regenerated plantlets of finger millet was confirmed by ISSR primers. Overall, the present work developed a robust and reliable procedure for the establishment of efficient and reproducible regeneration through the shoot apex that will be useful for the genetic improvement of this crop. The genetic enhancement of these millets as well as the successful creation of transgenic plant varieties modified for resistance to biotic and abiotic challenges in the near future would be aided by this study.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix Metalloproteinases in Glioma: Drivers of Invasion and Therapeutic Targets. 基质金属蛋白酶在胶质瘤中的作用：侵袭的驱动因素和治疗靶点。

IF 2.7

BioTech Pub Date : 2025-04-15 DOI: 10.3390/biotech14020028

Ella E Aitchison, Alexandra M Dimesa, Alireza Shoari

{"title":"Matrix Metalloproteinases in Glioma: Drivers of Invasion and Therapeutic Targets.","authors":"Ella E Aitchison, Alexandra M Dimesa, Alireza Shoari","doi":"10.3390/biotech14020028","DOIUrl":"https://doi.org/10.3390/biotech14020028","url":null,"abstract":"Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteolytic enzymes that are crucial for the remodeling of the extracellular matrix, a process that is often co-opted by cancers, including brain tumors, to facilitate growth, invasion, and metastasis. In gliomas, MMPs contribute to a complex interplay involving tumor proliferation, angiogenesis, and immune modulation, thereby influencing tumor progression and patient prognosis. This review provides a comprehensive analysis of the roles of various MMPs in different types of gliomas, from highly malignant gliomas to metastatic lesions. Emphasis is placed on how the dysregulation of MMPs impacts tumor behavior, the association between specific MMPs and the tumor grade, and their potential as biomarkers for diagnosis and prognosis. Additionally, the current therapeutic approaches targeting MMP activity are discussed, exploring both their challenges and future potential. By synthesizing recent findings, this paper aims to clarify the broad significance of MMPs in gliomas and propose avenues for translational research that could enhance treatment strategies and clinical outcomes.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AsNAC Genes: Response to High Mercury Concentrations in Allium sativum Seed Clove. 大蒜种子丁香AsNAC基因对高汞浓度的响应。

IF 2.7

BioTech Pub Date : 2025-04-08 DOI: 10.3390/biotech14020027

Brenda Mendoza-Almanza, María de la Luz Guerrero-González, Marcos Loredo-Tovias, María Elena García-Arreola, Catarina Loredo-Osti, Erika Padilla-Ortega, Pablo Delgado-Sánchez

{"title":"AsNAC Genes: Response to High Mercury Concentrations in Allium sativum Seed Clove.","authors":"Brenda Mendoza-Almanza, María de la Luz Guerrero-González, Marcos Loredo-Tovias, María Elena García-Arreola, Catarina Loredo-Osti, Erika Padilla-Ortega, Pablo Delgado-Sánchez","doi":"10.3390/biotech14020027","DOIUrl":"https://doi.org/10.3390/biotech14020027","url":null,"abstract":"Heavy metal contamination in soils is a growing concern due to anthropogenic activities, and Allium sativum (garlic) has shown tolerance to mercury pollution. We analyzed the physiological and molecular responses of garlic cloves exposed to HgCl2 at 0, 5000, 23,000, and 46,000 mg/kg for 2, 3, and 4 h. The germination percentage was lower than 46,000 mg/kg Hg for 4 h. We also analyzed the expression levels of NAC transcription factors and found that AsNAC11 had higher expression at 46,000 mg/kg at 2 h; AsNAC17 was underexpressed and the maximum was at 2 h at 23,000 mg/kg. AsNAC20 had the highest expression (30 times more than the control) at 3 and 4 h with 23,000 mg/Kg. AsNAC27 showed the highest expression at 3 h with 23,000 mg/kg. The tissues exhibited a maximum Hg bioconcentration factor of 0.037 at 23,000 mg/kg, indicating moderate mercury absorption. However, at a concentration of 46,000 mg/kg, the BCF decreased to 0.023. Our in-silico analysis revealed that the analyzed AsNACs are associated with various abiotic stress responses. This study provides valuable insights into genes that could be utilized for genetic improvement to enhance crop resistance to mercury soil contamination.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Classical and Rising Approaches the Extraction and Utilization of Marine Collagen. 海洋胶原蛋白提取与利用的经典方法及新兴方法综述。

IF 2.7

BioTech Pub Date : 2025-04-03 DOI: 10.3390/biotech14020026

Cesia Deyanira Gutierrez-Canul, Luis Alfonso Can-Herrera, Emmanuel de Jesús Ramírez-Rivera, Witoon Prinyawiwatkul, Enrique Sauri-Duch, Victor Manuel Moo-Huchin, Emanuel Hernández-Núñez

{"title":"A Review of Classical and Rising Approaches the Extraction and Utilization of Marine Collagen.","authors":"Cesia Deyanira Gutierrez-Canul, Luis Alfonso Can-Herrera, Emmanuel de Jesús Ramírez-Rivera, Witoon Prinyawiwatkul, Enrique Sauri-Duch, Victor Manuel Moo-Huchin, Emanuel Hernández-Núñez","doi":"10.3390/biotech14020026","DOIUrl":"https://doi.org/10.3390/biotech14020026","url":null,"abstract":"This comprehensive review explores the extraction and utilization of marine collagen, a sustainable alternative to traditional mammalian sources. The review covers conventional extraction methods like acid and pepsin solubilization, highlighting their limitations and contributing to the search for improved efficiency and sustainability. It also delves into innovative extraction technologies, such as ultrasound-assisted extraction, deep eutectic solvents, and supercritical carbon dioxide, showing their potential to revolutionize the field. The significance of collagen hydrolysis in generating bioactive peptides with diverse functionalities is also discussed, emphasizing their potential applications in various sectors. By providing an analysis of marine collagen extraction and its implications, this review presents a perspective for leveraging this valuable bioresource, promoting a circular economy, and satisfying the increasing demand for high-quality collagen in diverse industries.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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