Food Chemistry Molecular Sciences最新文献_第2页

Performance difference of enrofloxacin monoclonal antibody and nanobody elicited from the same immunogen and the underlying molecular mechanism 同种免疫原诱导的恩诺沙星单克隆抗体与纳米体的性能差异及其分子机制

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fochms.2026.100359

Yujie Chen , Zhaoxiang Wang , Qingqing He , Haiyan Xing , Simin Shen , Rui Feng , Yixuan Wu , Baomin Wang , Qing X. Li

{"title":"Performance difference of enrofloxacin monoclonal antibody and nanobody elicited from the same immunogen and the underlying molecular mechanism","authors":"Yujie Chen , Zhaoxiang Wang , Qingqing He , Haiyan Xing , Simin Shen , Rui Feng , Yixuan Wu , Baomin Wang , Qing X. Li","doi":"10.1016/j.fochms.2026.100359","DOIUrl":"10.1016/j.fochms.2026.100359","url":null,"abstract":"<div><div>Enrofloxacin (ENR) is a widely used fluoroquinolone antibiotic in animal husbandry, aquaculture, and humans. Here, a monoclonal antibody (mAb 2D3) and a nanobody (Nb22) against ENR were generated with the same immunogen. Nb22 had the nanobody common property of greater stability in harsh conditions, but its assay sensitivity was approximately 30-fold lower than that of mAb 2D3. Nb22 showed better selectivity, which the cross-reactivity to each of ENR analogs was less than or equal to that of mAb 2D3. The VH and VL gene sequences were amplified from the hybridoma cell line 2D3. Molecular docking revealed that mAb 2D3 had stronger hydrogen bonds and formed a flat and wide binding pocket to accommodate other analogs of ENR. The average recoveries of ENR from milk, milk powder, egg and fish determined by mAb 2D3 and Nb22 based ic-ELISAs ranged from 77.7% to 119% and 88.2% to 116%, respectively. This study confirmed that the direct application of nanobody in immunoassay is no better than the conventional monoclonal antibody. Improving the sensitivity of nanobody is an essential prerequisite for taking advantage of its stability and specificity.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100359"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated analysis of phenotypes and whole-transcriptome data for the preliminary exploration of molecular regulatory networks influencing beef quality 综合分析表型和全转录组数据，初步探索影响牛肉品质的分子调控网络

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 DOI: 10.1016/j.fochms.2026.100428

Mengli Yang , Xue Bai , Yuan Liu , Hongen Chu , Bei Cai , Runjun Yang , Lupei Zhang , Fen Li , Yun Ma

{"title":"Integrated analysis of phenotypes and whole-transcriptome data for the preliminary exploration of molecular regulatory networks influencing beef quality","authors":"Mengli Yang , Xue Bai , Yuan Liu , Hongen Chu , Bei Cai , Runjun Yang , Lupei Zhang , Fen Li , Yun Ma","doi":"10.1016/j.fochms.2026.100428","DOIUrl":"10.1016/j.fochms.2026.100428","url":null,"abstract":"<div><div>Guyuan cattle are a unique indigenous genetic resource in Northwest China, characterized by favorable roughage utilization efficiency and meat production potential. However, the molecular mechanisms governing their meat quality traits remain poorly elucidated. In this exploratory study, we characterized meat quality phenotypes and transcriptomic profiles in Guyuan cattle and Wagyu cattle, which differ significantly in intramuscular fat (IMF) content. Phenotypic comparisons (<em>n</em> = 6) revealed significant interbreed divergences in slaughter performance, IMF content, drip loss, moisture content, shear force, fatty acid composition, amino acid profile, and muscle fiber type. Through integrated whole-transcriptome sequencing (<em>n</em> = 3) and phenotypic analysis, and based on differential expression analysis coupled with phenotype correlation screening, we preliminarily identified 37 differentially genes associated with fat deposition (16), shear force (11), and muscle development (10). Based on seed region complementarity, phenotypic correlation, and signaling pathway enrichment analysis, we predicted potential target genes and constructed 16 co-expression networks. It should be noted that this study is limited by a small sample size, and the multi-omics data are associative in nature, which does not allow for causal inference. Therefore, the above genes and networks are predictive and have not undergone independent functional validation. These findings offer preliminary, exploratory insights into the potential molecular mechanisms associated with key meat quality traits in Guyuan cattle, and serve as a reference for future functional gene validation, genetic improvement of indigenous yellow cattle, and related industrial applications.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100428"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148183651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivar-driven variations in physicochemical properties and volatile organic compound profiles of strawberry (Fragaria × ananassa Duch.) 草莓（Fragaria × ananassa Duch.）理化性质和挥发性有机物分布的品种驱动变化

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2025-12-30 DOI: 10.1016/j.fochms.2025.100346

Zhen-Peng Zhou, Li-Yuan Zhao, Jing-Yao Sun, Qing-Yun Li, Bing-Bing Cai, Xin-Xin Wang

{"title":"Cultivar-driven variations in physicochemical properties and volatile organic compound profiles of strawberry (Fragaria × ananassa Duch.)","authors":"Zhen-Peng Zhou, Li-Yuan Zhao, Jing-Yao Sun, Qing-Yun Li, Bing-Bing Cai, Xin-Xin Wang","doi":"10.1016/j.fochms.2025.100346","DOIUrl":"10.1016/j.fochms.2025.100346","url":null,"abstract":"<div><div>Strawberry (<em>Fragaria × ananassa</em> Duch<em>.</em>) is an important economic crop all around the world. A new developed cultivar ‘Baozhu’ which has a similar fruit shape with ‘Hongyan’ is not yet proved to be a different genotype. The main purpose of the present study was to confirm the differences in genotypes and quality and volatile compounds of the two cultivars. The results showed that ‘Baozhu’ cultivar exhibited 39.6 % higher fruit weight, 35.1 % higher total soluble solids, 29.5 % higher firmness, 25.0 % higher total acidity, 45.0 % higher soluble sugars compared to ‘Hongyan’ cultivar (<em>p</em> < 0.05). A total of 51 volatile compounds were identified in the strawberries using headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS), and esters were the most abundant compounds, followed by aldehydes and alcohols. The results of chemometrics analysis reveal that ‘Baozhu’ and ‘Hongyan’ strawberries can be distinctly separated from each other. A panel of 28 volatiles with VIP > 1 and <em>p</em> < 0.05 were identified as discriminant factors for ‘Baozhu’ and ‘Hongyan’. Furthermore, ‘Baozhu’ cultivar had significantly more stomata than the ‘Hongyan’ cultivar. A clear and positive correlation was observed among quality, volatile and stomata number. The current findings contribute to the commercial value assessment of the ‘Baozhu’ cultivar at an industrial level, formulating a paradigm for improvement of daily eating pattern and driving the development of genetic tools for improvement of flavor.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100346"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetracycline residue alters the nutritional quality and bioactive composition of soybean sprouts: Evidence from transcriptomic and rhizosphere microbiota analyses 四环素残留改变豆芽的营养品质和生物活性成分：来自转录组学和根际微生物群分析的证据

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2025-12-31 DOI: 10.1016/j.fochms.2025.100345

Ting Cai , Jie Yao , Hongmei Jiang , Jie Zou , Ting Xia , Xinyue Mou , Shan Zhang , Xiao Tan , Jie Tang , Wenliang Xiang

{"title":"Tetracycline residue alters the nutritional quality and bioactive composition of soybean sprouts: Evidence from transcriptomic and rhizosphere microbiota analyses","authors":"Ting Cai , Jie Yao , Hongmei Jiang , Jie Zou , Ting Xia , Xinyue Mou , Shan Zhang , Xiao Tan , Jie Tang , Wenliang Xiang","doi":"10.1016/j.fochms.2025.100345","DOIUrl":"10.1016/j.fochms.2025.100345","url":null,"abstract":"<div><div>Antibiotic residues in edible crops have become an increasing food safety concern, yet their impacts on crop nutritional quality and bioactive composition remain poorly understood. Here, we investigated the effects of tetracycline, a widely used antibiotic in soil–vegetable systems, on the growth and nutritional quality and bioactive composition of soybean sprouts. Results showed tetracycline exposure significantly inhibited sprout growth and nutrient accumulation in a dose-dependent manner, with high concentrations reducing vitamin C, total flavonoids, and coumestrol contents by approximately 50 %, 30 %, and 43 %, respectively. Transcriptomic analysis revealed that these related declines were associated with disruptions in carbon, amino acid, and lipid metabolism, as well as in flavonoid and coumestrol biosynthesis pathways. In parallel, rhizosphere microbiota analysis showed that tetracycline reshaped microbial community structure by reducing nitrogen-cycling-related taxa (<em>Dokdonella</em>, <em>Acidibacter</em>) and enriching resistant genera (<em>Acinetobacter)</em>, which were significantly correlated with changes in sprout nutritional quality and bioactive composition. Together, these results demonstrate that tetracycline residues drive substantial losses of nutritional and bioactive composition in edible crops through coordinated metabolic and microbiome-mediated mechanisms, revealing an underappreciated pathway by which antibiotic contamination threatens crop nutritional value and food quality.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100345"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lychee peel extract and chitosan synergistically delay mango ripening: Molecular insights 荔枝皮提取物和壳聚糖协同延缓芒果成熟：分子的见解

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2026-01-13 DOI: 10.1016/j.fochms.2026.100355

Zhiwei Wu , Qinghua Qiao , Zhen Wang , Tiancui Shang , Shifang Wu , PengPeng He , Zhisheng Lin , Zhenxin Ren

{"title":"Lychee peel extract and chitosan synergistically delay mango ripening: Molecular insights","authors":"Zhiwei Wu , Qinghua Qiao , Zhen Wang , Tiancui Shang , Shifang Wu , PengPeng He , Zhisheng Lin , Zhenxin Ren","doi":"10.1016/j.fochms.2026.100355","DOIUrl":"10.1016/j.fochms.2026.100355","url":null,"abstract":"<div><div>Although lychee peel extract (LPE) is rich in bioactive compounds, its potential for postharvest fruit preservation remains unexplored. We hypothesised that LPE would act synergistically with chitosan (CH) to delay mango ripening by simultaneously modulating cell wall integrity, pigment metabolism, and hormone signaling pathways. Here, we demonstrate that chitosan combined with lychee peel extract (CHL) delays mango ripening through a multi-targeted mechanism. Specifically, CHL outperformed chitosan alone by significantly suppressing peel yellowing, maintaining fruit firmness, and reducing decay over 12 days of storage. Integrated transcriptomic and metabolomic analyses revealed that LPE reprogrammed ripening-associated pathways by (1) upregulating cell wall remodeling genes (CSLE1, XTH23) to stabilize pectin architecture, (2) retaining chlorophyll via suppressed CRTISO and PSY (carotenoid synthesis) and enhanced CHLP (chlorophyll biosynthesis), and (3) decoupling sugar-acid dynamics through γ-aminobutyric acid (GABA) and succinic acid accumulation. Notably, LPE attenuated ethylene-auxin- abscisic acid (ABA) crosstalk by downregulating ripening-specific transcription factors (ERF003, bZIPs) while activating stress-responsive WRKYs. These findings establish LPE as a sustainable alternative to synthetic preservatives, leveraging agricultural byproducts for eco-friendly fruit preservation.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100355"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Usage of nanobody-beta-galactosidase fusion in immunoassays and its application in detecting a peanut allergen 纳米体- β -半乳糖苷酶融合在免疫检测中的应用及其在花生过敏原检测中的应用

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2026-01-18 DOI: 10.1016/j.fochms.2026.100357

Yuzhu Zhang , Shilpa R. Bhardwaj , Mathis Carrere , Xiaohua He , Tengchuan Jin , Yixiang Xu

{"title":"Usage of nanobody-beta-galactosidase fusion in immunoassays and its application in detecting a peanut allergen","authors":"Yuzhu Zhang , Shilpa R. Bhardwaj , Mathis Carrere , Xiaohua He , Tengchuan Jin , Yixiang Xu","doi":"10.1016/j.fochms.2026.100357","DOIUrl":"10.1016/j.fochms.2026.100357","url":null,"abstract":"<div><div>Small-sized nanobodies (NBs) offer many advantages over traditional antibodies and antibody fragments. β-Galactosidase (β-gal) has been used as a detection enzyme in immunoassays when horseradish peroxidase (HRP) or alkaline phosphatase (ALP) could not be used. However, more research is needed to fully realize the benefits of using β-gal in immunoassays. This study fused a previously isolated NB specific to peanut allergen Ara h 3 (Nb16) with the tetrameric <em>Escherichia coli</em> β-gal. Kinetic signals generated using ONPG demonstrated the advantage of using β-gal in ELISA experiments. Peanut allergen Ara h 3 was successfully detected with the Nb16-β-gal, with a detection limit of 0.3 ppm, outperforming detection with the same NB and HRP. For detecting peanut proteins in baked foods, the detection limit was better than 1.56 ppm. Stable signals produced with S-Gal/X-Gal showed the benefits of using β-gal in immunoblots. The readily available, stable β-gal substrates and the ease of recombinant production of NB-β-gal chimeras are among the advantages of using β-gal over HRP and ALP as detection enzymes in immunoassays.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100357"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs from colostrum and mature cow milk: High-throughput sequencing and functional enrichment 初乳和成熟牛奶中的microrna：高通量测序和功能富集

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2025-12-08 DOI: 10.1016/j.fochms.2025.100337

Abdelmoneim H. Ali , Zain Najjar , Shao-Quan Liu , Mutamed Ayyash

{"title":"MicroRNAs from colostrum and mature cow milk: High-throughput sequencing and functional enrichment","authors":"Abdelmoneim H. Ali , Zain Najjar , Shao-Quan Liu , Mutamed Ayyash","doi":"10.1016/j.fochms.2025.100337","DOIUrl":"10.1016/j.fochms.2025.100337","url":null,"abstract":"<div><div>As vital regulators of newborn development, milk-derived microRNAs (miRNAs) have gained increasing attention recently. However, the variation in miRNAs profile among colostrum and mature milk remains unclear. We hypothesized that miRNAs content of colostrum and mature cow milk differs significantly, reflecting stage-specific biological functions related to immune priming and metabolic adaptation. Herein, high-throughput small RNA sequencing was performed to compare miRNAs profile in colostrum and mature cow milk. A total of 353 and 390 miRNA precursors were respectively detected in colostrum and mature milk, with diverse expression profiles. Colostrum displayed higher quantities of immune-related miRNAs, such as <em>let-7</em>, <em>miR-21</em>, and <em>miR-146</em>, whereas mature milk was enriched in metabolic regulation-related miRNAs, such as <em>miR-29b</em>, <em>miR-210</em>, and <em>miR-378</em>. Differential expression analysis showed 159 miRNAs substantially upregulated in colostrum and 38 in mature milk. Functional enrichment and pathway analysis revealed that mature milk miRNAs were associated with energy metabolism and tissues remodeling, while colostrum miRNAs were involved in early immune signaling and developmental activities. These findings support our hypothesis that miRNA expression patterns are temporally controlled across lactation and may serve as molecular indicators of milk functionality. This knowledge could be leveraged to enhance newborn nutrition strategies and design functional dairy products enriched with stage-specific miRNAs.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100337"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145711810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A key SNP in the flavonoid biosynthesis pathway gene CsaV3_6G009030 is highly correlated with cucumber fruit bitterness 类黄酮生物合成途径基因CsaV3_6G009030的一个关键SNP与黄瓜果实苦味高度相关

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2026-05-25 DOI: 10.1016/j.fochms.2026.100424

Qianli Lu , Chuanxing Zhang , Hongyu Chen , Yue Yu , Zhimin Wang , Dayong Wei , Jiaqi Zou , Hongcheng Zhang , Hebing Wang , Qinglin Tang

{"title":"A key SNP in the flavonoid biosynthesis pathway gene CsaV3_6G009030 is highly correlated with cucumber fruit bitterness","authors":"Qianli Lu , Chuanxing Zhang , Hongyu Chen , Yue Yu , Zhimin Wang , Dayong Wei , Jiaqi Zou , Hongcheng Zhang , Hebing Wang , Qinglin Tang","doi":"10.1016/j.fochms.2026.100424","DOIUrl":"10.1016/j.fochms.2026.100424","url":null,"abstract":"<div><div>Cucumber fruit bitterness is a prominent issue affecting fruit quality and seriously compromises the commercial value of cucumbers. In this study, near-isogenic lines (NILs) were developed through six consecutive backcross generations using the bitter cucumber inbred line ‘5’ (P2) as the recurrent parent and the non-bitter cucumber inbred line ‘WK15’ (P1) as the donor parent, generating a BC6F2 population. Bulk segregant RNA-seq (BSR-seq) combined with metabolomics analysis was performed to screen for candidate regions and metabolic pathways significantly associated with fruit bitterness. A 5.8 Mb (2.7–8.5 Mb) candidate region for bitterness was mapped to chromosome 6. Functional analysis of the target region revealed significant enrichment of the flavonoid biosynthesis pathway. Metabolic network analysis indicated that six flavonoid differential metabolites, including 4-methoxycinnamic acid and 2′-hydroxychalcone, were significantly enriched in bitter cucumbers, and their expression levels were highly consistent with the bitter phenotype. Through gene functional annotation and SNP validation, a key gene, <em>CsaV3_6G009030</em>, involved in the flavonoid biosynthesis pathway was identified, and a functional dCAPS marker was developed based on this gene. This study reveals a potential association between flavonoid metabolism and bitterness formation in cucumber, providing a theoretical basis and technical support for the further application of marker-assisted selection in breeding high-quality, non-bitter cucumber varieties.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100424"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148183649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated genomic, transcriptomic, and metabolomic profile analysis reveals molecular mechanism underlying meat quality traits in Chinese Kangle chickens 综合基因组学、转录组学和代谢组学分析揭示了中国康乐鸡肉质性状的分子机制

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2025-12-31 DOI: 10.1016/j.fochms.2025.100350

Qin He , Liang Xiong , Shuting Fang , Yan Chen , Jinge Ma , Zhangfeng Wang , Yanping Wu , Jiguo Xu , Xinwei Xiong

{"title":"Integrated genomic, transcriptomic, and metabolomic profile analysis reveals molecular mechanism underlying meat quality traits in Chinese Kangle chickens","authors":"Qin He , Liang Xiong , Shuting Fang , Yan Chen , Jinge Ma , Zhangfeng Wang , Yanping Wu , Jiguo Xu , Xinwei Xiong","doi":"10.1016/j.fochms.2025.100350","DOIUrl":"10.1016/j.fochms.2025.100350","url":null,"abstract":"<div><div>The local Chinese Kangle chicken breed has plump muscles, tender-tasting meat, and strong disease resistance. We aimed to identify muscle metabolites and associated regulatory genes that determine Kangle chicken muscle quality and flavor by elucidating the as yet unknown underlying molecular regulatory mechanism through omics-based analyses. Phenotype analysis and genome-wide association study (GWAS) showed that intramuscular fat (IMF) exhibited a high coefficient of variation and identified two SNPs that reached genome-wide significance levels (<em>P</em> < 1.23 × 10<sup>−6</sup>), with QTLs affecting IMF located on chromosomes 1, 2, 4, and 7. Transcriptome analysis screened 201 differential expression genes between chickens with high and low IMF content. Among these, the expression level of the FYVE-type zinc finger containing (<em>PIKFYVE</em>) gene located in the significant QTL region was significantly downregulated in the High-IMF group, which was validated by qRT-PCR. Metabolomics identified 17 intergroup differential metabolites, and the High-IMF group was significantly enriched in vitamin B6 metabolism and glycine, serine, and threonine metabolism. Differentially expressed gene and metabolite analyses in breast muscles and pathway enrichment and co-occurring network analyses showed that <em>PIKFYVE</em> impacted IMF. Its expression significantly and negatively correlated with most lipid metabolites. This study is the first to establish <em>PIKFYVE</em> as the primary regulatory gene of IMF in Kangle chickens. It provides crucial and valuable practical insights into genetically breeding high-quality meat chicken breeds.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100350"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Tiroler Bergkäse PDO cheese: A multimethodological approach 表征的梯罗勒Bergkäse PDO奶酪：一个多方法的方法

IF 4.7

Food Chemistry Molecular Sciences Pub Date : 2026-06-01 Epub Date: 2025-12-08 DOI: 10.1016/j.fochms.2025.100336

Hannah Innerbichler , Alexander Trockenbacher , Alexander Höller , Sabine Scholl-Bürgi , Lorenzo Del Vecchio , Martina Cirlini , Jürgen König , Katrin Bach

{"title":"Characterization of Tiroler Bergkäse PDO cheese: A multimethodological approach","authors":"Hannah Innerbichler , Alexander Trockenbacher , Alexander Höller , Sabine Scholl-Bürgi , Lorenzo Del Vecchio , Martina Cirlini , Jürgen König , Katrin Bach","doi":"10.1016/j.fochms.2025.100336","DOIUrl":"10.1016/j.fochms.2025.100336","url":null,"abstract":"<div><div>Tiroler Bergkäse PDO is a traditional Austrian hard cheese from Tyrol, a product of great regional significance. This study aimed to describe Tiroler Bergkäse PDO by comparing it with Bergkäse without PDO from Tyrol and a Stilfser type cheese from South Tyrol. To account for variability, three cheese wheels from three production days were analyzed for each cheese. Multimethodological characterization included quantitative Polymerase Chain Reaction, Ion Exchange Chromatography, and Headspace-Solid Phase Microextraction/Gas Chromatography-Mass Spectrometry. The characterization Tiroler Bergkäse PDO should impart potentially unique features. Multivariate analysis of the bacterial composition, free amino acid content, and volatile fraction revealed differences between all cheeses (α = 0.05). A triangle test confirmed significant differences, with more pronounced differences between the Bergkäse type cheeses and the Stilfser type cheese. The Stilfser type showed distinct differences in bacterial composition, amino acid content, and volatile profile, making it less similar to the Bergkäse type cheeses. Multivariate analysis also revealed differences between the PDO and non-PDO Bergkäse which is characterized by high levels of L. <em>delbrueckii</em> due to its starter culture, and a high free amino acid content from longer ripening, alongside elevated levels of hexanal and acetoin. In contrast, Bergkäse without PDO shows high levels of 2,3-butanediol and 2-nonanone, alongside high levels of L. <em>mesenteroides</em>, <em>L. lactis</em> subsp. <em>lactis</em>, and L. <em>lactis</em> subsp. <em>cremoris</em>. It was possible to distinguish between Tiroler Bergkäse PDO from non-PDO cheeses by microbial, amino acid, and volatile profiles and support the use of advanced methods to characterize regional foods.</div></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"12 ","pages":"Article 100336"},"PeriodicalIF":4.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0