Journal of Medical Microbiology and Infectious Diseases最新文献

{"title":"A Shot at Dendritic Cell-Based Vaccine Strategy against HIV-1","authors":"M. S. Larijani, S. M. Sadat, A. Bolhassani, Amitis Ramezani","doi":"10.29252/jommid.7.4.89","DOIUrl":"https://doi.org/10.29252/jommid.7.4.89","url":null,"abstract":"*Correspondences Email1: amitisramezani@hotmail.com Email2: mehdi_sadat@pasteur.ac.ir Tel/Fax1: +98 21 64112240 Tel/Fax2: +98 21 64112812 Introduction: Despite considerable efforts to control AIDS pandemic, it is still one of the significant infectious concerns worldwide. The advance in medical research has led to the development of highly active antiretroviral therapy with a considerable effect to suppress the disease. However, an effective vaccine capable of eradication the HIV pandemic is not available yet. Failure to develop a prophylactic vaccine diverted the efforts to clinical trials of therapeutic vaccines. Methods: Here, we review different approaches to dendritic cell-based HIV therapeutic vaccines. We have summarized the dendritic cell-based trials as HIV therapeutic vaccination, registered in the United States clinical trial database. Results and Conclusion: The strategies applied in the clinical trials were mostly of low success rates; however, by using dendritic cell therapy, they could trigger the host immune response against HIV-1 infections.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83807506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran","authors":"Maryam Khalili-Samani, M. Barati, Navid Mirmohammadsadegh, M. Amin, Ali Samadikuchaksaraei","doi":"10.29252/jommid.7.4.127","DOIUrl":"https://doi.org/10.29252/jommid.7.4.127","url":null,"abstract":"Department of Drug and Food Control, and Pharmaceutical Quality Assurance Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Faculty of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran; The Institute of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran, Iran; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78078723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Quinolone Resistance in Escherichia coli Isolates Recovered from Urine and Feces of Patients with Acute or Recurrent Urinary Tract Infection","authors":"Hossein Norouzian, N. Shahrokhi, S. Sabeti, S. Bouzari, M. Pooya","doi":"10.29252/jommid.7.4.120","DOIUrl":"https://doi.org/10.29252/jommid.7.4.120","url":null,"abstract":"*Correspondences Email1: m_pooya@pasteur.ac.ir Email2: bouzari@pasteur.ac.ir Tel: +98 21 64112220 Fax: +98 21 64112803 Introduction: Antibiotic resistance, especially in Gram-negative uropathogens such as Escherichia coli, is the main barrier to treat urinary tract infection (UTI). In recent years, the dramatically increased resistance of E. coli to quinolones, a group of widely used antibiotics, has become a significant concern. Methods: In this descriptive crosssectional study, we collected 261 E. coli isolates from the urine and stool samples of patients, referred to or hospitalized at Loghman hospital in Tehran, Iran, with either acute or recurrent UTI. The susceptibility testing for quinolones was performed by the disk diffusion method according to the recent protocols. Results: The frequency of resistant E. coli isolates was higher against nalidixic acid than ciprofloxacin and norfloxacin (67.8% vs. 48.7% and 44.1% respectively). When comparing acute and recurrent phases of UTI, in the urine samples, no significant difference was seen in the frequency of resistant isolates against nalidixic acid and norfloxacin, while this frequency against ciprofloxacin was significantly higher in recurrent UTI (68% vs. 48.2%). However, in the stool samples, the frequency of resistant isolates against nalidixic acid was higher in recurrent UTI (77.1% vs. 55.7%), while no significant difference was seen against ciprofloxacin and norfloxacin in these phases. Conclusion: Regarding the antibiotic type and frequency of the administration, the resistance pattern of E. coli to quinolones seems to differ in acute and recurrent phases of UTI.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83148632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Delivery of HIV-1 Nef Antigen by Histidine-rich nona-arginine and Latarcin 1 peptide","authors":"F. Namazi, A. Bolhassani, S. M. Sadat, S. Irani","doi":"10.29252/jommid.7.4.107","DOIUrl":"https://doi.org/10.29252/jommid.7.4.107","url":null,"abstract":"*Correspondence Email: A_bolhasani@pasteur.ac.ir Tel: +98 21 64112240 Fax: +98 21 66465132 Introduction: The Nef accessory protein is an attractive antigenic candidate in the development of HIV-1 DNAor protein-based vaccines. The most crucial disadvantage of DNA and protein-based vaccines is their low immunogenicity, which can be improved by cell-penetrating peptides (CPPs) as effective carrier molecules. Methods: In this study, the HIV-1 Nef protein was generated in the Escherichia coli expression system for in vitro delivery using a novel CPP, Latarcin 1 peptide, in a non-covalent manner. Also, the Histidine-rich nona-arginine peptide was utilized to transfer the HIV1 Nef gene. The size, morphology, and zeta potential of the complexes were evaluated by scanning electron microscopy (SEM) and Zetasizer. The efficiency of cell transfection was studied using a fluorescence microscopy and flow cytometry for the DNA/CPP complexes and western blot analysis for the protein/CPP complexes. Results: The Nef protein generated in the BL21 strain migrated as a dominant band of ~30 kDa in SDS-PAGE. The SEM data confirmed the formation of stable complexes with a size below 200 nm. MTT assay demonstrated that the complexes did not represent any considerable cytotoxic effect compared to untreated HEK-293T cells. The results of fluorescence microscopy, flow cytometry, and western blotting revealed that the Nef DNA and protein constructs could be significantly transfected into HEK-293T cell line using these CPPs. Conclusion: These data suggest that the Histidine-rich nonaarginine peptide and Latarcin 1 peptide as CPPs can be considered as a promising approach in the development of the HIV-1 vaccine for gene or protein delivery.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80482292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity Determination of Recombinant lysostaphin Immobilized on Glass Surfaces Modified by Cold Atmospheric Plasma on Staphylococcus aureus","authors":"Gelareh Ehsani, Foad Fahmide, D. Norouzian, S. Atyabi, P. Ehsani","doi":"10.29252/jommid.7.4.132","DOIUrl":"https://doi.org/10.29252/jommid.7.4.132","url":null,"abstract":"*Correspondences Email1: p_ehsani@yahoo.com Email2: mohammadatyabi@yahoo.com Tel: +98 21 64112219 Fax: +98 21 66465132 Introduction: Staphylococcus aureus is a source of nosocomial infections and one of the significant concerns in patients with indwelling devices. Lysostaphin is a bacterially produced endopeptidase with a unique activity on S. aureus. Plasma, the fourth state of the material, consists of charged ions, free electrons, and activated neutral species. Biomedical applications of cold plasma are rapidly growing due to its capacity to treat heat-sensitive objects such as polymeric materials and biological samples. It activates surfaces by etching them to stabilize proteins. The direct effect of cold atmospheric plasma on the eradication of microorganisms have been investigated. However, there is no report on immobilizing antibiotic agents. Methods: In this study, the lysostaphin protein was expressed and purified using Ni-NTA column, then the purified enzyme was immobilized on glass surfaces pretreated with cold atmospheric plasma for 150 s, 200 s, and 300 s. The antimicrobial activity of immobilized lysostaphin on S. aureus was approved by in vitro analysis. Results: The 300 s plasma treatment confirmed to be the best time arrangement for more lysostaphin immobilization, shown by Atomic Force Microscopy. Conclusion: Our results showed that passive adsorption to the treated surface does not affect the structure and subsequent antimicrobial function of the recombinant protein compared to the standard proteins.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75936903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure Evaluation of IroN for Designing a Vaccine against Escherichia Coli, an In Silico Approach","authors":"F. Sefid, Roghayyeh Baghban, Z. Payandeh, B. Khalesi, M. M. Gomari","doi":"10.29252/jommid.7.4.93","DOIUrl":"https://doi.org/10.29252/jommid.7.4.93","url":null,"abstract":"Department of Medical Genetics, Shahid Sadoughi University of Medical Science, Yazd, Iran; Department of Biology, Science and Art University, Yazd, Iran; Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Science, Tabriz, Iran; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extention Organization (AREEO), Karaj, Iran; Department of Medical Biotechnology, Faculty of Paramedical, Iran University of Medical Science, Tehran, Iran","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88489117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Agglutination Assays in Diagnosis of Brucellosis in Golestan Province, North of Iran","authors":"B. Khodabakhshi, Abdollah Abbasi, Mobina Torabi Rostami, H. Joshaghani, G. Roshandel","doi":"10.29252/jommid.7.4.116","DOIUrl":"https://doi.org/10.29252/jommid.7.4.116","url":null,"abstract":"The clinical symptoms of brucellosis are similar to a wide range of diseases; hence, reliable diagnostic and laboratory methods are required to identify the causative agent. Iran is an endemic region of brucellosis, and many patients are misdiagnosed due to the nature of the infection. In this study, we aimed to evaluate and compare the use of the conventional Wright test and quantitative polymerase chain reaction (qPCR) for the diagnosis of brucellosis. Methods: Diagnosis of brucellosis was performed using serological tests and PCR amplification of a gene encoding 31-kDa immunogenic Brucella abortus protein (BCSP31). Data were analyzed using the Chi-square test. Results: Brucellosis was diagnosed in 45 (69.23%) and 22 (38.8%) patients using the Wright test and qRT-PCR, respectively. The results of Wright and qRT-PCR assays were consistent in patients with negative results (90%). Moreover, qRT-PCR detected brucellosis in 25% of patients with Wright test titers <1/160, while 55.2% of the patients were positive with titers ≥1/160. No significant association was detected between positive PCR results and age, gender, and clinical symptoms. Conclusion: qRT-PCR showed a reliable diagnostic method capable of detecting the infection in suspected individuals with negative Wright results or with Wright test titers <1/160. Also, the positive qRT-PCR assays were in agreement with the Wright test titer. Regarding the financial and availability issues as well as technical problems, the agglutination test remains the preferred method in Iran.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86712262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolution and pro-resolving lipid mediators in Leishmania infection","authors":"N. Seyed, S. Rafati","doi":"10.29252/jommid.7.3.61","DOIUrl":"https://doi.org/10.29252/jommid.7.3.61","url":null,"abstract":"*Correspondence Email: Negarse@gmail.com Tel: +98 [21] 64112810 Fax: +98 [21] 64112810 The acute inflammatory response is the body’s natural reaction to inciting stimuli, including trauma and pathogens. Well-known pro-inflammatory metabolites take control of this reaction to recruit the leukocytes into the inflamed tissue. These cells professionally ingest and kill the invading pathogens and clear the debris of dead or injured cells. This further signals the tissue regeneration and gain of function, another active process mediated by newly uncovered anti-inflammatory metabolites that downregulate the active inflammation. These molecules and their cognate receptors are novel targets for the treatment of chronic inflammatory diseases. Although not very well understood, these mediators are suspected of supporting intracellular parasite survival (as Leishmania parasite) and are worth further investigation for innovative therapeutic interventions.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"362 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76168952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Epidemics of Cutaneous Leishmaniasis in Iran: Operational Aspects, Management and Implemented Control Approaches","authors":"I. Sharifi, Mohammad Reza Aflatoonian, Z. Babaei, F. Sharifi, A. Keyhani, Ehsan Salarkia, A. Khosravi, A. Khamesipour, M. Mohebali, A. Nadim, M. Bamorovat","doi":"10.29252/jommid.7.3.52","DOIUrl":"https://doi.org/10.29252/jommid.7.3.52","url":null,"abstract":"Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran; Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran; Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; Center for Research and Training in Skin Diseases & Leprosy, Tehran University of Medical Sciences, Tehran, Iran; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88518591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LeiSHield-MATI consortium: An international project to understand and combat cutaneous leishmaniasis through research and innovation staff exchange","authors":"F. Bahrami, S. Rafati","doi":"10.29252/jommid.7.3.85","DOIUrl":"https://doi.org/10.29252/jommid.7.3.85","url":null,"abstract":"For long, it had become evident to leishmaniases researchers that the pathological outcomes of infections such as cutaneous leishmaniasis (CL) are affected by complex interactions of multiple factors, including the genetic diversities of the parasite and its phlebotomine vector, the immunocompetence of the human hosts, the mammalian reservoirs and last but not least, the environmental conditions, which favors the propagation of the parasite in transition [1]. Hence, simplistic assumptions based on data obtained from small animal models or monitoring the fluctuations of a few available biomarkers [2] have not been able to elevate our understanding of the mechanisms that shape or control CL pathologies. This has left us with no approved vaccination or new therapeutic for any forms of CL. The above shortcomings were addressed during a two-day Leishmania panel session within the 2016 meeting of Pasteur Institutes of the MATI region (Morocco, Algeria, Tunisia, and Iran) with the participation of Institut Pasteur in Paris, which was held at Pasteur Institute of Iran in Tehran. The exchange of ideas among the experts in that panel led to the proposition of a multilateral yet integrated research approach toward finding preventive and therapeutic means against the shared CL problem of this region. The main elements of this approach were based on “Leishmania genomics”, “host immune responses”, and “vector and transmission” thematics. Moreover, the issue of the social impact of CL was also raised, with the aim to increase public awareness on societal calamities caused by this neglected disease [3]. Upon a request by the Institut Pasteur International Network (IPIN) and with tremendous contributions of all the involved Leishmania researchers and in particular, Dr. Gerard Spaeth (head of Molecular Parasitology and Signaling Unit of Institut Pasteur), the topics and workpackages defined in the aforementioned Leishmania panel were developed into a well-defined two-year research project. This proposal entitled \"LeiSHield-MATI Project A multidisciplinary integrative approach toward understanding clinical, molecular and socio-economic factors underlying cutaneous leishmaniasis across Morocco, Algeria, Tunisia and Iran in collaboration with Institut Pasteur International Network”, was evaluated and accepted by external reviewers under supervision of IPIN in 2017 and was consequently approved for funding by each involved Pasteur Institute in the consortium (€20,000 for 2 years per Institute).","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80997869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}