Journal of Medical Microbiology and Infectious Diseases最新文献_第8页

Detection of Staphylococcus aureus Enterotoxin A (SEA) Using Dot-ELISA in Milk Samples Dot-ELISA法检测牛奶样品中金黄色葡萄球菌肠毒素A (SEA)

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-10-01 DOI: 10.29252/JOMMID.8.4.132

Haniyeh Golafrouz, H. Ahari, S. Anvar, D. Shahbazzadeh, Tehran-Iran. Bio therapeutic Molecules Lab.

{"title":"Detection of Staphylococcus aureus Enterotoxin A (SEA) Using Dot-ELISA in Milk Samples","authors":"Haniyeh Golafrouz, H. Ahari, S. Anvar, D. Shahbazzadeh, Tehran-Iran. Bio therapeutic Molecules Lab.","doi":"10.29252/JOMMID.8.4.132","DOIUrl":"https://doi.org/10.29252/JOMMID.8.4.132","url":null,"abstract":"Introduction: Staphylococcus aureus enterotoxin A (SEA) is one of the most common causes of staphylococcal food poisoning. Due to the simplicity and no requirement for laboratory apparatuses, dot-ELISA is a choice method for detecting Staphylococcal enterotoxins. The present study aimed to develop a dot-ELISA for the detection of SEA. Methods: Nitrocellulose membranes were coated with the SEA antibody and blocked by the addition of 3% bovine serum albumin (BSA) blocking buffer. After 1 h incubation and washing the membranes, milk samples and the positive control (SEA, 50 ng/ml) were added to the membranes and incubated for 1 h. The membranes were then washed and incubated for 45 min with HRP-conjugated SEA, followed by the addition of TMB. Results: Our dot-ELISA could detect amounts of ≥ 50 ng/ml of SEA in the milk samples. Of the 30 raw milk samples randomly purchased from dairy product stores in District 3, Tehran, 5 (16%) contained SEA ≥ 50 ng/ml by the dot-ELISA. Conclusion : The dot-ELISA showed to be a reliable method for the preliminary screening of milk samples for SEA contamination. This method is cost-effective, fast, and does not require an ELISA-reader device.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76483386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva sylvestris and Capsella bursa-pastoris against Methicillin-Resistant Staphylococcus aureus 洋甘菊、西洋花和荠菜乙醇提取物对耐甲氧西林金黄色葡萄球菌的抑菌活性评价

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-10-01 DOI: 10.29252/JOMMID.8.4.127

Shahrbanoo Sadat Sadat, A. Azari, M. Mazandarani

{"title":"Evaluation of Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva sylvestris and Capsella bursa-pastoris against Methicillin-Resistant Staphylococcus aureus","authors":"Shahrbanoo Sadat Sadat, A. Azari, M. Mazandarani","doi":"10.29252/JOMMID.8.4.127","DOIUrl":"https://doi.org/10.29252/JOMMID.8.4.127","url":null,"abstract":"*Correspondence Email: ania_783@yahoo.com Tel: + 989111777377 Fax: +9817321353000 Introduction: The study aimed to determine the antibacterial activity of ethanolic extract of Matricaria chamomilla (chamomile), Malva sylvestris, and Capsella bursa-pastoris against Methicillin-resistant Staphylococcus aureus (MRSA) isolated from clinical specimens. Methods: The plants were collected from Ziarat Village, southern heights of Gorgan, and the required parts were separated and then thoroughly dried in the shade. After grinding, extraction was performed by the maceration method. The extract was dried at 37°C for 24 h. A concentration of 50 mg/ml of each extract was obtained in 10 ml 5% dimethyl sulfoxide and sterilized. For the antibacterial assay, agar well diffusion and broth microdilution methods were used. Results: Our results showed no inhibitory effect for the ethanolic extracts of M. sylvestris and C. bursa-pastoris against the MRSA isolates in both antibacterial assays. The chamomile flower extract showed antibacterial activity against the 20 MRSA isolates at 50 and 25 mg/ml concentrations. The extract from chamomile leaves demonstrated an inhibitory effect on the 7 MRSA isolates. The extracts from chamomile flowers demonstrated MIC and MBC at a concentration of 6.25 and 12.5 mg/ml for most MRSA isolates, while these values for the extracts from chamomile leaves were 12.5 and 25 mg/ml for a few MRSA isolates, respectively. Conclusion: In this study, the ethanolic flower extract of chamomile showed significant antibacterial activity against the MRSA isolates. Hence, this extract may be an alternative to antibiotic therapy and a good option to control infections caused by MRSA and pathogenic bacteria.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86270090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Phenotypic Detection of Beta-lactamases among Proteus mirabilis, Enterobacter cloacae, and Citrobacter freundii Isolates from Urinary Samples in Gorgan, Northeast Iran 伊朗东北部戈尔干地区尿液中奇异变形杆菌、阴沟肠杆菌和弗伦地柠檬酸杆菌分离株β -内酰胺酶的表型检测

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-10-01 DOI: 10.29252/JOMMID.8.4.161

Ameneh Fenderski, A. Azari, T. Dadgar

{"title":"Phenotypic Detection of Beta-lactamases among Proteus mirabilis, Enterobacter cloacae, and Citrobacter freundii Isolates from Urinary Samples in Gorgan, Northeast Iran","authors":"Ameneh Fenderski, A. Azari, T. Dadgar","doi":"10.29252/JOMMID.8.4.161","DOIUrl":"https://doi.org/10.29252/JOMMID.8.4.161","url":null,"abstract":"Introduction: The data on members of the genera Proteus , Pseudomonas , Enterobacter , Citrobacter, and Staphylococcus as the etiologic agents of urinary tract infections (UTIs) is not much. This study investigated the frequency of various beta-lactamases in urine isolates of Proteus mirabilis , Enterobacter cloacae, and Citrobacter freundii in Gorgan, Golestan province. Methods: A total of 632 urine samples were collected from hospitalized patients in a teaching hospital. The samples were cultured on blood agar and Eosin Methylene blue agar and incubated overnight at 37°C. The cultures with a ≥105 CFU/mL bacterial count were defined as positive for UTI. Bacteria identification was performed using standard biochemical methods and the API20E enteric identification system. The antibiotic resistance pattern was determined by the Kirby-Bauer disk diffusion method, and a phenotypic confirmatory test was used for detecting ESBL, MBL, and AmpC beta-lactamases producers. Results: Out of 632 samples, 317 (50.1%) were positive for UTIs, and 27 (8.5%), 21 (6.6%), and 12 (3.7%) were positive for Enterobacter cloacae , Citrobacter freundii, and Proteus mirabilis isolates, respectively. All the isolates were sensitive to piperacillin-tazobactam and colistin. The prevalence of ESBL and AmpC beta-lactamases in P. mirabilis isolates was higher than the other isolates, but No MBL producers were detected. Conclusions: In this study, the high frequency of ESBL and AmpC beta-lactamases in P. mirabilis isolates may suggest an increasing trend in resistance to cephalosporins and monobactams, which could have a significant impact on the management and treatment of UTI caused by this organism. Therefore, continuous monitoring is required to control the spread of β-lactamase-producing isolates in different geographical areas.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75150368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Investigation of Chemical Composition, Antibacterial and Antioxidant Activity of Thymus daenensis and Thymus eriocalyx Essential Oils against Human Pathogenic Bacteria 胸腺精油对人致病菌的化学成分、抑菌活性及抗氧化活性研究

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-10-01 DOI: 10.29252/JOMMID.8.4.148

M. Alamholo

{"title":"Investigation of Chemical Composition, Antibacterial and Antioxidant Activity of Thymus daenensis and Thymus eriocalyx Essential Oils against Human Pathogenic Bacteria","authors":"M. Alamholo","doi":"10.29252/JOMMID.8.4.148","DOIUrl":"https://doi.org/10.29252/JOMMID.8.4.148","url":null,"abstract":"Introduction: Plant essential oils can be used as alternative agents for the treatment of antibiotic-resistant pathogenic bacteria. This study aimed to investigate the chemical composition, antibacterial, and antioxidant activity of Thymus eriocalyx and Thymus daenensis essential oils against Gram-positive and Gram-negative human pathogenic bacteria. Methods: The aerial part of Thymus eriocalyx and Thymus daenensis in the full flowering stage were collected from West Azerbaijan Province (Urmia), Iran, in 2014. We obtained the essential oils using a Clevenger device. The disc diffusion method was used to determine the antibacterial activity of the essential oils against 15 Gram-positive and Gram-negative PTCC and ATCC bacterial standards. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were measured by microdilution broth method in 96-well plate and free radical scavenging activity by 2,2-diphenyl-1-picrylhydrazyl. Chemical analysis of the essential oils performed out by gas chromatography connected to Mass spectrometry. Results: Eleven (94.06%) and seven (90.76%) compounds were identified in T. eriocalyx and T. daenensis essential oils, respectively. The major components of T. eriocalyx essential oil were thymol (37.8%) and α-terpineol (14.91%), and of T. daenensis were thymol (52.36%) and carvacrol (16.72%). T. eriocalyx essential oil showed the highest activity against B. cereus with MIC of 0.93 μg mL - and MBC of 1.87 μg mL - . The most potent radical scavenging activity was also obtained for T. daenensis essential oil . Conclusion: Essential oil components of T. eriocalyx and T. daenensis may have the potential to be used as antimicrobial agents against antibiotic-resistant pathogenic bacteria.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91201530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Prevalence and Antibiotic Resistance Pattern of Streptococcus genus and Other Pathogens Isolated from Throat Culture Samples of Patients in Fatemeh Al-Zahra Hospital of Sari, Iran 伊朗萨里市Fatemeh Al-Zahra医院患者咽喉培养标本中链球菌属及其他病原体的流行及耐药模式

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-10-01 DOI: 10.29252/JOMMID.8.4.143

M. Nazari, Marzieh Ebrahimi, Niloofar Mobarezpour, A. Sepehr

{"title":"Prevalence and Antibiotic Resistance Pattern of Streptococcus genus and Other Pathogens Isolated from Throat Culture Samples of Patients in Fatemeh Al-Zahra Hospital of Sari, Iran","authors":"M. Nazari, Marzieh Ebrahimi, Niloofar Mobarezpour, A. Sepehr","doi":"10.29252/JOMMID.8.4.143","DOIUrl":"https://doi.org/10.29252/JOMMID.8.4.143","url":null,"abstract":"*Correspondence Email: mtebrahimi@ymail.com Tel: +989011955851 Fax: +982122222222 Introduction: Tracheal tubes are among the primary means of infection transmission in hospitals. Therefore, identifying microbial agents transmitted via this route is necessary to control and prevent these infections. This study aimed to investigate the prevalence of pharyngeal-contaminating microorganisms and their antibiotic resistance pattern. Methods: In this cross-sectional study, we used 117 pharyngeal swabs samples obtained from patients referred to Fatemeh Zahra Hospital of Sari, Iran, in 2018. The Samples were obtained using the sterile cotton swab from the throat and then cultured in the sheep blood agar. The positive colonies for the alpha-hemolytic test were subcultured on the Mueller-Hinton agar for further assays, including the susceptibility to optochin, catalase test, Gram's polychromatic stain, microscopic examination, pyrrolidonyl aminopeptidase (PYR) test, sensitization to bacitracin, and latex agglutination assay. The antibiotic susceptibility assay was performed using the agar disk diffusion method according to CLSI-2018 guidelines. The results were analyzed using SPSS 16.0 software and descriptive statistical methods. Results: The frequency of Streptococcus pneumoniae and Streptococcus pyogenes was 13.7% and 9.4%, respectively. However, the highest frequency belonged to Pseudomonas aeruginosa (14.5%), and the lowest to Citrobacter spp. (0.9%). Conclusion: Our results indicated increased antibiotic resistance in streptococcal strains due to inappropriate prescriptions and antibiotic misuse. Therefore, recognizing and controlling the contributing factors and the rational use of antibiotics can be very important.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84200679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Fast and Straightforward Method for the Purification of Anti- Immunoglobulin G (IgG) for Coombs Wright Assay 库姆斯·赖特试验中抗免疫球蛋白G (IgG)的一种快速、简便的纯化方法

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-10-01 DOI: 10.29252/JOMMID.8.4.155

Z. Panahi, V. Kia, Mitra Moghiman, D. Doroud, R. Shokri, M. Paryan

{"title":"A Fast and Straightforward Method for the Purification of Anti- Immunoglobulin G (IgG) for Coombs Wright Assay","authors":"Z. Panahi, V. Kia, Mitra Moghiman, D. Doroud, R. Shokri, M. Paryan","doi":"10.29252/JOMMID.8.4.155","DOIUrl":"https://doi.org/10.29252/JOMMID.8.4.155","url":null,"abstract":"were applied to purify IgG. SDS-PAGE and Bradford protein content assay was conducted to evaluate the quality and the concentration of the purified IgG. Rabbits were weekly injected with different amounts of the protein four times. Then, sera were obtained from the immunized mice, and total IgG was purified. Finally, the Coombs Wright test was performed on samples from brucellosis patients to validate purified IgG antibody quality. Results: Electrophoresis and Bradford assay results showed that the purified protein had considerable high purity and quantity. Protein bands of reducing and the non-reducing SDS-PAGE showed high purity of the protein along with a protein yield of 2.2 mg/L. Coombs Wright tests using the rabbit anti-human serum had a comparable result with available commercial anti-human immunoglobulin. Conclusion: The results indicated that our method for the purification of IgG was suitable for anti-human globulin preparation. This antibody can also be used in clinical diagnostic tests such as Coombs Wright, cross-match, and blood types evaluation with weak Rh or Du antigens. ,","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80368154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Host Species on Hatchability of Fasciola hepatica and Fasciola gigantica Eggs from Sheep and Cattle 宿主物种对羊、牛肝片吸虫和巨型片吸虫卵孵化率的影响

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-10-01 DOI: 10.29252/JOMMID.8.4.166

Sara Larki, M. Jalali, Saeed Goodarzi, M. Zamani

{"title":"Effect of Host Species on Hatchability of Fasciola hepatica and Fasciola gigantica Eggs from Sheep and Cattle","authors":"Sara Larki, M. Jalali, Saeed Goodarzi, M. Zamani","doi":"10.29252/JOMMID.8.4.166","DOIUrl":"https://doi.org/10.29252/JOMMID.8.4.166","url":null,"abstract":"Introduction: Successful development of free-living stages of parasitic helminths depends on larva ability to survive, develop, and hatch. In this study, we aimed to study the host role in the hatching process of Fasciola species. Methods: Fasciola hepatica and Fasciola gigantica eggs were collected from adult worms that originated from naturally infected sheep and cattle livers and were incubated at 26±1°C for 15 days. The percentage of hatched and developed eggs were obtained for each isolate under a light microscope. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) was applied to identify the F. hepatica and F. gigantica species. Results: Our findings showed no significant differences in the development rates of F. gigantica and F. hepatica eggs in sheep (69.32% and 72.71%) and cattle (73.56% and74.69%). However, the rates of hatched eggs of F. gigantica and F. hepatica originated from cattle (69.19% and 62.36%) were almost twice the rates in sheep (31.69% and 32.59%), indicating a significant difference. Conclusion: This study demonstrated that host species significantly affect the hatching of Fasciola eggs as the hatching rates of F. gigantica and F. hepatica originated from cattle were higher than those taken from sheep did not affect their larval development. Thus, in addition to environmental factors, the hatching phenomenon is influenced by host species.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83259774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Enhancement of SARS-CoV-2 Receptor Binding Domain -CR3022 Human Antibody Binding Affinity via in Silico Engineering Approach 基于硅工程方法增强SARS-CoV-2受体结合域-CR3022人抗体结合亲和力

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-08-14 DOI: 10.21203/rs.3.rs-56411/v1

F. Sefid, Z. Payandeh, G. Azamirad, B. Mansoori, B. Baradaran, M. Sadeghi

{"title":"Enhancement of SARS-CoV-2 Receptor Binding Domain -CR3022 Human Antibody Binding Affinity via in Silico Engineering Approach","authors":"F. Sefid, Z. Payandeh, G. Azamirad, B. Mansoori, B. Baradaran, M. Sadeghi","doi":"10.21203/rs.3.rs-56411/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-56411/v1","url":null,"abstract":"\u0000 Background: The nCoV-2019 is a cause of COVID-19 disease. The surface spike glycoprotein (S), which is necessary for virus entry through the intervention of the host receptor and it mediates virus-host membrane fusion, is the primary coronavirus antigen (Ag). The angiotensin-converting enzyme 2 (ACE2) is reported to be the effective human receptor for SARS-CoVs 2. ACE2 receptor can be prevented by neutralizing antibodies (nAbs) such as CR3022 targeting the virus receptor-binding site. Considering the importance of computational docking, and affinity maturation we aimed to find the important amino acids of the CR3022 antibody (Ab). These amino acids were then replaced by other amino acids to improve Ab-binding affinity to a receptor-binding domain (RBD) of the 2019-nCoV spike protein. Finally, we measured the binding affinity of Ab variants to the Ag. Result: Our findings disclosed that several variant mutations could successfully improve the characteristics of the Ab binding compared to the normal antibodies. Conclusion: The modified antibodies may be possible candidates for stronger affinity binding to Ags which in turn can affect the specificity and sensitivity of antibodies.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85006417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Class I Integron and Antimicrobial Resistance Profile of Klebsiella pneumonia isolates in Babol, North of Iran 伊朗北部巴博勒地区肺炎克雷伯菌I类整合子及耐药谱调查

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-01-01 DOI: 10.29252/jommid.8.1.24

Parisa Sabbagh, Azadeh Ferdosi-Shahandashti, Mehdi Rajabnia, A. Maali, E. Shahandashti

引用次数: 1

Molecular Study of Occult Hepatitis C Infection among Iranian Hemophilia Patients Treated with Direct-acting Antiviral Agents 直接抗病毒药物治疗伊朗血友病患者隐匿性丙型肝炎感染的分子研究

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2020-01-01 DOI: 10.29252/jommid.8.1.1

Elnaz Agi, S. Asghari, A. Namvar, Niloofar Khairkhah, Niloofar Naderi, A. Anvar, A. Saraji, A. Bolhassani

{"title":"Molecular Study of Occult Hepatitis C Infection among Iranian Hemophilia Patients Treated with Direct-acting Antiviral Agents","authors":"Elnaz Agi, S. Asghari, A. Namvar, Niloofar Khairkhah, Niloofar Naderi, A. Anvar, A. Saraji, A. Bolhassani","doi":"10.29252/jommid.8.1.1","DOIUrl":"https://doi.org/10.29252/jommid.8.1.1","url":null,"abstract":"*Correspondence Email: A_bolhasani@pasteur.ac.ir Tel: +98 21 66953311 Ext. 2240 Fax: +98 21 66465132 Introduction: A new pathological form of HCV named as occult HCV infection (OCI) has been recently characterized by the presence of HCV RNA in liver biopsy and/or peripheral blood mononuclear cell specimens (PBMCs) and the absence of detectable circulating HCV RNA in plasma samples. In this study, we investigated the presence of HCV RNA in PBMCs and plasma samples of 100 hemophilia patients with negative serum HCV RNA. Methods: One hundred hemophilia participants receiving IFN-free direct-acting antivirals (DAAs) regimens as a treatment of HCV infection participated in this study. PBMCs were separated with Ficoll before RNA extraction. The HCV genotypes of the positive specimens were also analyzed by RT-PCR assay. Finally, data analysis was performed by SPSS software. Results: Our data revealed that out of 100 hemophilia patients, three (3%, 95% CI: 0.006-0.085) were positive for OCI, showing a significant association between OCI and genotype3/drug regimens (p = 0.0203). There was no significant increase at ALT and AST levels in patients with OCI. Moreover, a genotype difference was observed between plasma and PBMCs samples of 1% (1/100) of patients. Conclusion: Generally, HCV genotyping in PBMCs along with plasma subtyping before beginning the therapy is vital due to the possibility of OCI detection.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74915518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0