Journal of Medical Microbiology and Infectious Diseases最新文献_第7页

Expression of a Novel HIV-1 Gag-Pol-Env-Nef-Rev Multi-Epitope Construct in Escherichia coli 新型HIV-1 Gag-Pol-Env-Nef-Rev多表位结构在大肠杆菌中的表达

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-06-01 DOI: 10.52547/jommid.9.2.62

E. Akbari, S. Ajdari, E. M. Ardakani, Elnaz Agi, V. Khalaj, A. Bolhassani

{"title":"Expression of a Novel HIV-1 Gag-Pol-Env-Nef-Rev Multi-Epitope Construct in Escherichia coli","authors":"E. Akbari, S. Ajdari, E. M. Ardakani, Elnaz Agi, V. Khalaj, A. Bolhassani","doi":"10.52547/jommid.9.2.62","DOIUrl":"https://doi.org/10.52547/jommid.9.2.62","url":null,"abstract":"of multiepitope protein as an candidate to this study, the codon-optimized encoding sequence of the designed multi-epitope construct (Gag-Pol-Env-Nef-Rev) was synthesized and subcloned into the pET-24a (+) expression vector. Then, expression of the target antigen was evaluated in E. coli BL21 (DE3) and Rosetta strains under different conditions (temperature, optical density/ OD 600 , isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration, and time). Finally, the expression of the Gag-Pol-Env-Nef-Rev multi-epitope protein was confirmed using SDS-PAGE and western blot analysis. Results: The highly conserved and immunodominant T-cell epitopes of HIV-1 Gag, Pol, Env, Nef, and Rev proteins were used to prepare a novel Gag-Pol-Env-Nef-Rev multi-epitope construct. The gag-pol-env-nef-rev gene was successfully sub-cloned in pET-24a (+) vector and subsequently expressed in BL21 (DE3) E. coli strain under optimized conditions (1 mM IPTG, 16 h post-induction, OD 600 = 0.6, and 37ºC). A clear band of ~ 35 kDa was detected by western blotting using an anti-His antibody, indicating the successful expression of our target multi-epitope protein. Conclusion: Expression of the recombinant HIV-1 multi-epitope protein was optimized in a bacterial system. The expressed protein will be purified to use as a multi-epitope protein vaccine candidate in the future.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74132976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Dehydrozingerone on Growth, Biofilm Formation, and Ergosterol Biosynthesis of Candida albicans 脱氢锌酮对白色念珠菌生长、生物膜形成和麦角甾醇生物合成的影响

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-06-01 DOI: 10.52547/jommid.9.2.76

Z. Jahanshiri, S. Nejatbakhsh

引用次数: 0

Protection and Immune Responses Elicited by rSAG1-PLGA Nanoparticles in C57BL/6 Against Toxoplasma gondii rSAG1-PLGA纳米颗粒对C57BL/6抗刚地弓形虫的保护作用及免疫应答

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/JOMMID.9.1.38

M. Allahyari, Samira Amiri, A. Vatanara, Majid Golkar

引用次数: 0

Transient Gene Expression: an Approach for Recombinant Vaccine Production 瞬时基因表达:一种生产重组疫苗的方法

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/JOMMID.9.1.46

M. Habibi-Pirkoohi, A. Shahriari, M. H. G. Parizipour

{"title":"Transient Gene Expression: an Approach for Recombinant Vaccine Production","authors":"M. Habibi-Pirkoohi, A. Shahriari, M. H. G. Parizipour","doi":"10.52547/JOMMID.9.1.46","DOIUrl":"https://doi.org/10.52547/JOMMID.9.1.46","url":null,"abstract":"The production of recombinant vaccines in green plants is an attractive and promising topic in genetic engineering. However, the stable transformation of green plants is a time-consuming, costly, and labor-intensive practice. Moreover, public concerns about genetically modified plants put another limitation on the development and release of transgenic plant-based recombinant vaccines. These shortcomings were addressed by developing transient gene expression systems that allow researchers to investigate candidate recombinant vaccines quickly without tedious work and high costs. A comprehensive literature review was used to gather relevant information. This approach has received much attention in various recombinant vaccine production platforms, including mammalian cell culture, insect cell culture, yeast expression systems, and, more importantly, in plant hosts. Due to their simplicity and efficiency, transient gene expression systems are now widely used to validate gene constructs and transgene expression within plant tissues. This paper describes the concept of transient gene expression and discusses the significant advantages of this approach for producing recombinant vaccines. Notably, the major types of transient gene expression viz. agroinfiltration, viral-based systems, and application of naked plasmid in plant cell culture are introduced, and some examples illustrate the pros and cons of each system. Our literature review also discusses some practical notes on the successful application of this system to provide a more comprehensive image of transient gene expression applicability in green plants. As a whole, this review contributes to the existing literature by shedding more light on various aspects of transient gene expression that have not been addressed thoroughly yet.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86345170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison of HBV-DNA Levels with Biochemical and Microbiological Parameters for Chronic Hepatitis Evaluation, Bursa, Turkey HBV-DNA水平与生化和微生物参数在慢性肝炎评估中的比较，Bursa，土耳其

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/jommid.9.1.17

Sanem Karadağ Geçgel

引用次数: 1

The Effect of Silibinin on the Expression of TLR7, ISG15, and SOCS1 in Peripheral Blood Mononuclear Cells of Hepatitis C Infected Patients in Comparison with Interferon-α 水飞蓟宾对丙型肝炎患者外周血单核细胞TLR7、ISG15、SOCS1表达的影响及与干扰素-α的比较

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/JOMMID.9.1.12

R. Dowran, S. Hosseini, M. Fattahi, J. Sarvari

{"title":"The Effect of Silibinin on the Expression of TLR7, ISG15, and SOCS1 in Peripheral Blood Mononuclear Cells of Hepatitis C Infected Patients in Comparison with Interferon-α","authors":"R. Dowran, S. Hosseini, M. Fattahi, J. Sarvari","doi":"10.52547/JOMMID.9.1.12","DOIUrl":"https://doi.org/10.52547/JOMMID.9.1.12","url":null,"abstract":"Background: Silibinin (silibinin A) is the most active silymarin component, which acts both as a hepatoprotective [1] and an antiviral agent. The present study investigated the silibinin effect on IFN-related innate immune genes in PBMCs from HCV-infected patients. Method: 22 chronic HCV patients, including 10 IFN responders and 12 non-responders, were included. Their isolated PBMCs were treated for 6 hours in the presence of silibinin, IFN-α, or their combination. The transcription level of TLR7, ISG15, and SOCS1 genes was compared using real-time PCR. Result: Our result showed that IFN-α induced a significant up-regulation of TLR7 and ISG15 in PBMCs of both responder and non-responder groups. Nevertheless, the SOCS1 gene was not significantly changed in the non-responder group (P=0.32). The combination of IFNα- and silibinin showed a similar pattern to IFN-α alone. By itself, silibinin did not leave a significant change on the expression level of the studied genes . Conclusion: The results indicated that silibinin did not enhance or suppress the expression level of TLR7, ISG15, and SOCS1 genes. Therefore, it has been suggested that its anti-inflammatory role might be devoid of IFN pathways.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83782033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contamination of Raw Herbs with Parasitic Protozoa and Helminths in Shushtar City, Southwestern Iran 伊朗西南部舒什塔尔市生药材受寄生原虫和蠕虫污染的调查

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/JOMMID.9.1.32

Seyede Manizhe Heidar Nejadi, A. Abdoli

{"title":"Contamination of Raw Herbs with Parasitic Protozoa and Helminths in Shushtar City, Southwestern Iran","authors":"Seyede Manizhe Heidar Nejadi, A. Abdoli","doi":"10.52547/JOMMID.9.1.32","DOIUrl":"https://doi.org/10.52547/JOMMID.9.1.32","url":null,"abstract":"IntroductionIntestinal parasites are among the most prevalent foodborne diseases worldwide, and raw vegetables and herbs are among the primary sources of human infection by these parasites. This study aimed to investigate the prevalence of parasitic contamination of fresh herbs in Shushtar, Khuzestan Province, Southwest of Iran.MethodsIn this study, 129 herb samples from various farms were collected and washed with water. The washing waters were centrifuged, and the resulting sediments were examined by formol-ether concentration and Sheatherchr('39')s sugar flotation procedure, as well as a wet smear and Ziehl-Neelsen staining.ResultsAmong the 129 samples, 73.6% (n=95) showed contamination with at least one parasite, including trophozoite like amoebae (52.6%), followed by Giardia lamblia (14.7%), Cryptosporidium spp. (2.1%), Blastocystis sp. (21%), free-living nematodes larvae (3.1%), Trichostrongilid nematodes (1.05%), Ascaris lumericoids eggs (2.1%), Hymenolepis spp. (2.1%) and Taeniid eggs (1.05%).ConclusionA high prevalence rate of parasitic contaminations of herbs in Shushtar necessitates proper washing of herbs and vegetables by consumers to prevent parasitic infections.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82786880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Evaluation of Multiplicity of Infection (MOI) and Harvesting Time on the Production of CVS-11 Strain of Rabies Virus in BSR Cell Line 狂犬病病毒CVS-11株在BSR细胞株上的感染多重性(MOI)和收获时间评价

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/JOMMID.9.1.25

M. Rahpeyma, R. Bashar

引用次数: 0

Transmission of COVID-19 between Animals and Humans: A challenge for the Scientists COVID-19在动物和人类之间的传播:对科学家的挑战

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/JoMMID.9.1.1

E. Mostafavi, Z. Eftekhari, N. Jabbari, P. Gheibi

引用次数: 1

Comparison of Antioxidant Activity of Dracocephalum polychaetum Bornm and Nepeta cataria L. and Their Effect on Probiotic Bacteria in a Simulated Gastrointestinal Environment 在模拟胃肠道环境下，龙头草和荆芥抗氧化活性的比较及其对益生菌的影响

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2021-03-01 DOI: 10.52547/JOMMID.9.1.5

F. Shahdadi, Maryam Payandeh, A. S. Sardoei

引用次数: 0