Protection and Immune Responses Elicited by rSAG1-PLGA Nanoparticles in C57BL/6 Against Toxoplasma gondii

Abstract

, blank PLGA, and one group kept unvaccinated. The characteristics of PLGA nanoparticles, the amounts of produced IFN-γ, IL-10, specific anti-Toxoplasma IgGs, and the conferred protection against infection by T. gondii RH tachyzoite were assessed. Results: rSAG1-PLGA nanoparticles shared a z-average of about 450nm with negative Zeta potential. Compared with the negative control group, the mice vaccinated with rSAG1-PLGA nanoparticles produced significantly higher amounts of IFN-γ, specific anti- T. gondii IgG antibodies and higher titer of IgG2a, which resulted in longer survival times. Conclusion: The efficiency of rSAG1-PLGA nanoparticles in inducing humoral and cellular responses and consequently partial protection against acute toxoplasmosis in C57BL/6 was confirmed.