Environmental Science and Ecotechnology最新文献

{"title":"Geobatteries in environmental biogeochemistry: Electron transfer and utilization","authors":"Shihao Cui ,&nbsp;Rui Wang ,&nbsp;Qing Chen ,&nbsp;Lorenzo Pugliese ,&nbsp;Shubiao Wu","doi":"10.1016/j.ese.2024.100446","DOIUrl":"https://doi.org/10.1016/j.ese.2024.100446","url":null,"abstract":"<div><p>The efficiency of direct electron flow from electron donors to electron acceptors in redox reactions is significantly influenced by the spatial separation of these components. Geobatteries, a class of redox-active substances naturally present in soil–water systems, act as electron reservoirs, reversibly donating, storing, and accepting electrons. This capability allows the temporal and spatial decoupling of redox half-reactions, providing a flexible electron transfer mechanism. In this review, we systematically examine the critical role of geobatteries in influencing electron transfer and utilization in environmental biogeochemical processes. Typical redox-active centers within geobatteries, such as quinone-like moieties, nitrogen- and sulfur-containing groups, and variable-valent metals, possess the potential to repeatedly charge and discharge. Various characterization techniques, ranging from qualitative methods like elemental analysis, imaging, and spectroscopy, to quantitative techniques such as chemical, spectroscopic, and electrochemical methods, have been developed to evaluate this reversible electron transfer capacity. Additionally, current research on the ecological and environmental significance of geobatteries extends beyond natural soil–water systems (e.g., soil carbon cycle) to engineered systems such as water treatment (e.g., nitrogen removal) and waste management (e.g., anaerobic digestion). Despite these advancements, challenges such as the complexity of environmental systems, difficulties in accurately quantifying electron exchange capacity, and scaling-up issues must be addressed to fully unlock their potential. This review underscores both the promise and challenges associated with geobatteries in responding to environmental issues, such as climate change and pollutant transformation.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"22 ","pages":"Article 100446"},"PeriodicalIF":14.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000607/pdfft?md5=405392aab693313bd59d790efc365d2f&pid=1-s2.0-S2666498424000607-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic governance: China's roadmap to improved health through climate and clean air actions","authors":"Tao Xue,&nbsp;Tong Zhu","doi":"10.1016/j.ese.2024.100447","DOIUrl":"https://doi.org/10.1016/j.ese.2024.100447","url":null,"abstract":"","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"20 ","pages":"Article 100447"},"PeriodicalIF":14.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000619/pdfft?md5=f0072d136b5de66717b25a3c5482bcbf&pid=1-s2.0-S2666498424000619-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keratinous and corneous-based products towards circular bioeconomy: A research review","authors":"Giovanni Davide Barone ,&nbsp;Irene Tagliaro ,&nbsp;Rodrigo Oliver-Simancas ,&nbsp;Matteo Radice ,&nbsp;Livia M. Kalossaka ,&nbsp;Michele Mattei ,&nbsp;Antonino Biundo ,&nbsp;Isabella Pisano ,&nbsp;Amparo Jiménez-Quero","doi":"10.1016/j.ese.2024.100444","DOIUrl":"10.1016/j.ese.2024.100444","url":null,"abstract":"<div><p>Keratins and corneous proteins are key components of biomaterials used in a wide range of applications and are potential substitutes for petrochemical-based products. Horns, hooves, feathers, claws, and similar animal tissues are abundant sources of α-keratin and corneous β-proteins, which are by-products of the food industry. Their close association with the meat industry raises environmental and ethical concerns regarding their disposal. To promote an eco-friendly and circular use of these materials in novel applications, efforts have focused on recovering these residues to develop sustainable, non-animal-related, affordable, and scalable procedures. Here, we review and examine biotechnological methods for extracting and expressing α-keratins and corneous β-proteins in microorganisms. This review highlights consolidated research trends in biomaterials, medical devices, food supplements, and packaging, demonstrating the keratin industry's potential to create innovative value-added products. Additionally, it analyzes the state of the art of related intellectual property and market size to underscore the potential within a circular bioeconomic model.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"22 ","pages":"Article 100444"},"PeriodicalIF":14.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000589/pdfft?md5=5a24035c671ccb5c42b24395a82d377a&pid=1-s2.0-S2666498424000589-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141961330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment","authors":"Lluís Bertrans-Tubau ,&nbsp;Sergio Martínez-Campos ,&nbsp;Julio Lopez-Doval ,&nbsp;Meritxell Abril ,&nbsp;Sergio Ponsá ,&nbsp;Victoria Salvadó ,&nbsp;Manuela Hidalgo ,&nbsp;Anna Pico-Tomàs ,&nbsp;Jose Luis Balcazar ,&nbsp;Lorenzo Proia","doi":"10.1016/j.ese.2024.100445","DOIUrl":"https://doi.org/10.1016/j.ese.2024.100445","url":null,"abstract":"<div><p>The overuse and misuse of antibiotics have accelerated the selection of antibiotic-resistant bacteria, significantly impacting human, animal, and environmental health. As aquatic environments are vulnerable to antibiotic resistance, suitable management practices should be adopted to tackle this phenomenon. Here we show an effective, nature-based solution for reducing antibiotic resistance from actual wastewater. We utilize a bioreactor that relies on benthic (biofilms) and planktonic microbial communities to treat secondary effluent from a small urban wastewater treatment plant (&lt;10,000 population equivalent). This treated effluent is eventually released into the local aquatic ecosystem. We observe high removal efficiency for genes that provide resistance to commonly used antibiotic families, as well as for mobile genetic elements that could potentially aid in their spread. Importantly, we notice a buildup of sulfonamide (<em>sul1</em> and <em>sul2</em>) and tetracycline (<em>tet(C)</em>, <em>tet(G)</em>, and <em>tetR</em>) resistance genes specifically in biofilms. This advancement marks the initial step in considering this bioreactor as a nature-based, cost-effective tertiary treatment option for small UWWTPs facing antibiotic resistance challenges.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"22 ","pages":"Article 100445"},"PeriodicalIF":14.0,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000590/pdfft?md5=1ef9cea825e211b8c0b97200670d6d4a&pid=1-s2.0-S2666498424000590-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141542010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strong metal-support interaction (SMSI) in environmental catalysis: Mechanisms, application, regulation strategies, and breakthroughs","authors":"Fuyuan Qi,&nbsp;Jianfei Peng,&nbsp;Zilu Liang,&nbsp;Jiliang Guo,&nbsp;Jiayuan Liu,&nbsp;Tiange Fang,&nbsp;Hongjun Mao","doi":"10.1016/j.ese.2024.100443","DOIUrl":"10.1016/j.ese.2024.100443","url":null,"abstract":"<div><p>The strong metal-support interaction (SMSI) in supported catalysts plays a dominant role in catalytic degradation, upgrading, and remanufacturing of environmental pollutants. Previous studies have shown that SMSI is crucial in supported catalysts' activity and stability. However, for redox reactions catalyzed in environmental catalysis, the enhancement mechanism of SMSI-induced oxygen vacancy and electron transfer needs to be clarified. Additionally, the precise control of SMSI interface sites remains to be fully understood. Here we provide a systematic review of SMSI's catalytic mechanisms and control strategies in purifying gaseous pollutants, treating organic wastewater, and valorizing biomass solid waste. We explore the adsorption and activation mechanisms of SMSI in redox reactions by examining interfacial electron transfer, interfacial oxygen vacancy, and interfacial acidic sites. Furthermore, we develop a precise regulation strategy of SMSI from systematical perspectives of interface effect, crystal facet effect, size effect, guest ion doping, and modification effect. Importantly, we point out the drawbacks and breakthrough directions for SMSI regulation in environmental catalysis, including partial encapsulation strategy, size optimization strategy, interface oxygen vacancy strategy, and multi-component strategy. This review article provides the potential applications of SMSI and offers guidance for its controlled regulation in environmental catalysis.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"22 ","pages":"Article 100443"},"PeriodicalIF":14.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000577/pdfft?md5=6ba288c77ca3ac4caff084f5d2614e8a&pid=1-s2.0-S2666498424000577-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141951399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"65% cover is the sustainable vegetation threshold on the Loess Plateau","authors":"Yi-ping Chen ,&nbsp;Kai-bo Wang ,&nbsp;Bo-jie Fu ,&nbsp;Yan-fen Wang ,&nbsp;Han-wen Tian ,&nbsp;Yi Wang ,&nbsp;Yi Zhang","doi":"10.1016/j.ese.2024.100442","DOIUrl":"https://doi.org/10.1016/j.ese.2024.100442","url":null,"abstract":"<div><p>Global temperatures will continue to increase in the future. The ∼640,000-km² Loess Plateau (LP) is a typical arid and semi-arid region in China. Similar regions cover ∼41% of the Earth, and its soils are some of the most severely eroded anywhere in the world. It is very important to understand the vegetation change and its ecological threshold under climate change on the LP for the sustainable development in the Yellow River Basin. However, little is known about how vegetation on the LP will respond to climate change and what is the sustainable threshold level of vegetation cover on the LP. Here we show that the temperature on the LP has risen 0.27 °C per decade over the past 50 years, a rate that is 30% higher than the average warming rate across China. During historical times, vegetation change was regulated by environmental factors and anthropogenic activities. Vegetation coverage was about 53% on the LP from the Xia Dynasty to the Spring and Autumn and Warring States period. Over the past 70 years, however, the environment has gradually improved and the vegetation cover had increased to ∼65% by 2021. We forecast future changes of vegetation cover on the LP in 2030s, in 2050s and in 2070s using SDM (Species Distribution Model) under Low-emission scenarios, Medium-emission scenarios and High-emission scenarios. An average value of vegetation cover under the three emission scenarios will be 64.67%, 62.70% and 61.47%, respectively. According to the historical record and SDM forecasts, the threshold level of vegetation cover on the LP is estimated to be 53–65%. Currently, vegetation cover on the LP has increased to the upper limit of the threshold value (∼65%). We conclude that the risk of ecosystem collapse on the LP will increase with further temperature increases once the vegetated area and density exceed the threshold value. It is urgent to adopt sustainable strategies such as stopping expanding vegetation area and scientifically optimizing the vegetation structure on the LP to improve the ecological sustainability of the Yellow River Basin.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"22 ","pages":"Article 100442"},"PeriodicalIF":14.0,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000565/pdfft?md5=1c264a25cf8cb314b35525a445a21dab&pid=1-s2.0-S2666498424000565-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141483422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorisation of industrial hemp (Cannabis sativa L.) residues and cheese whey into volatile fatty acids for single cell protein production","authors":"Carlo Moscariello,&nbsp;Silvio Matassa,&nbsp;Francesco Pirozzi,&nbsp;Giovanni Esposito,&nbsp;Stefano Papirio","doi":"10.1016/j.ese.2024.100439","DOIUrl":"10.1016/j.ese.2024.100439","url":null,"abstract":"<div><p>The production of single cell protein (SCP) using lignocellulosic materials stands out as a promising route in the circular bioeconomy transition. However, multiple steps are necessary for lignocellulosics-to-SCP processes, involving chemical pretreatments and specific aerobic cultures. Whereas there are no studies that investigated the SCP production from lignocellulosics by using only biological processes and microbial biomass able to work both anaerobically and aerobically. In this view, the valorisation of industrial hemp (<em>Cannabis sativa</em> L.) biomass residues (HBRs), specifically hurds and a mix of leaves and inflorescences, combined with cheese whey (CW) was investigated through a semi-continuous acidogenic co-fermentation process (co-AF). The aim of this study was to maximise HBRs conversion into VFAs to be further used as carbon-rich substrates for SCP production. Different process conditions were tested by either removing CW or increasing the amount of HBRs in terms of VS (i.e., two and four times) to evaluate the performance of the co-AF process. Increasing HBRs resulted in a proportional increase in VFA production up to 3115 mg HAc L⁻¹, with experimental production nearly 40% higher than theoretical predictions. The synergy between HBRs and CW was demonstrated, proving the latter as essential to improve the biodegradability of the former. The produced VFAs were subsequently tested as substrates for SCP synthesis in batch aerobic tests. A biomass concentration of 2.43 g TSS L⁻¹ was achieved with a C/N ratio of 5.0 and a pH of 9.0 after two days of aerobic fermentation, reaching a protein content of 42% (g protein per g TSS). These results demonstrate the overall feasibility of the VFA-mediated HBR-to-SCP valorisation process.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"21 ","pages":"Article 100439"},"PeriodicalIF":14.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266649842400053X/pdfft?md5=889e81d50253791b102694b88e119cc2&pid=1-s2.0-S266649842400053X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141413557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrite-driven anaerobic ethane oxidation","authors":"Cheng-Cheng Dang ,&nbsp;Yin-Zhu Jin ,&nbsp;Xin Tan ,&nbsp;Wen-Bo Nie ,&nbsp;Yang Lu ,&nbsp;Bing-Feng Liu ,&nbsp;De-Feng Xing ,&nbsp;Nan-Qi Ren ,&nbsp;Guo-Jun Xie","doi":"10.1016/j.ese.2024.100438","DOIUrl":"10.1016/j.ese.2024.100438","url":null,"abstract":"<div><p>Ethane, the second most abundant gaseous hydrocarbon in vast anoxic environments, is an overlooked greenhouse gas. Microbial anaerobic oxidation of ethane can be driven by available electron acceptors such as sulfate and nitrate. However, despite nitrite being a more thermodynamically feasible electron acceptor than sulfate or nitrate, little is known about nitrite-driven anaerobic ethane oxidation. In this study, a microbial culture capable of nitrite-driven anaerobic ethane oxidation was enriched through the long-term operation of a nitrite-and-ethane-fed bioreactor. During continuous operation, the nitrite removal rate and the theoretical ethane oxidation rate remained stable at approximately 25.0 mg NO₂^–N L⁻¹ d⁻¹ and 11.48 mg C₂H₆ L⁻¹ d⁻¹, respectively. Batch tests demonstrated that ethane is essential for nitrite removal in this microbial culture. Metabolic function analysis revealed that a species affiliated with a novel genus within the family Rhodocyclaceae, designated as '<em>Candidatus</em> Alkanivoras nitrosoreducens', may perform the nitrite-driven anaerobic ethane oxidation. In the proposed metabolic model, despite the absence of known genes for ethane conversion to ethyl-succinate and succinate-CoA ligase, '<em>Ca</em>. A. nitrosoreducens' encodes a prospective fumarate addition pathway for anaerobic ethane oxidation and a complete denitrification pathway for nitrite reduction to nitrogen. These findings advance our understanding of nitrite-driven anaerobic ethane oxidation, highlighting the previously overlooked impact of anaerobic ethane oxidation in natural ecosystems.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"21 ","pages":"Article 100438"},"PeriodicalIF":14.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000528/pdfft?md5=dfae899e89f6f60f9a583213ab0f39ec&pid=1-s2.0-S2666498424000528-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141396370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confounding associations between green space and outdoor artificial light at night: Systematic investigations and implications for urban health","authors":"Yang Liu ,&nbsp;Mei-Po Kwan ,&nbsp;Jianying Wang ,&nbsp;Jiannan Cai","doi":"10.1016/j.ese.2024.100436","DOIUrl":"10.1016/j.ese.2024.100436","url":null,"abstract":"<div><p>Excessive urbanization leads to considerable nature deficiency and abundant artificial infrastructure in urban areas, which triggered intensive discussions on people's exposure to green space and outdoor artificial light at night (ALAN). Recent academic progress highlights that people's exposure to green space and outdoor ALAN may be confounders of each other but lacks systematic investigations. This study investigates the associations between people's exposure to green space and outdoor ALAN by adopting the three most used research paradigms: population-level residence-based, individual-level residence-based, and individual-level mobility-oriented paradigms. We employed the green space and outdoor ALAN data of 291 Tertiary Planning Units in Hong Kong for population-level analysis. We also used data from 940 participants in six representative communities for individual-level analyses. Hong Kong green space and outdoor ALAN were derived from high-resolution remote sensing data. The total exposures were derived using the spatiotemporally weighted approaches. Our results confirm that the negative associations between people's exposure to green space and outdoor ALAN are universal across different research paradigms, spatially non-stationary, and consistent among different socio-demographic groups. We also observed that mobility-oriented measures may lead to stronger negative associations than residence-based measures by mitigating the contextual errors of residence-based measures. Our results highlight the potential confounding associations between people's exposure to green space and outdoor ALAN, and we strongly recommend relevant studies to consider both of them in modeling people's health outcomes, especially for those health outcomes impacted by the co-exposure to them.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"21 ","pages":"Article 100436"},"PeriodicalIF":14.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000504/pdfft?md5=678dc38e07c3792a4fce3dc4e699c58f&pid=1-s2.0-S2666498424000504-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141392824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing anaerobic digestion: Benefits of mild temperature transition from thermophilic to mesophilic conditions","authors":"Xingxing Zhang ,&nbsp;Pengbo Jiao ,&nbsp;Yiwei Wang ,&nbsp;Yinying Dai ,&nbsp;Ming Zhang ,&nbsp;Peng Wu ,&nbsp;Liping Ma","doi":"10.1016/j.ese.2024.100440","DOIUrl":"10.1016/j.ese.2024.100440","url":null,"abstract":"<div><p>Anaerobic digestion (AD) plays a significant role in renewable energy recovery. Upgrading AD from thermophilic (50–57 °C) to mesophilic (30–38 °C) conditions to enhance process stability and reduce energy input remains challenging due to the high sensitivity of thermophilic microbiomes to temperature fluctuations. Here we compare the effects of two decreasing-temperature modes from 55 to 35 °C on cell viability, microbial dynamics, and interspecies interactions. A sharp transition (ST) is a one-step transition by 20 °C d⁻¹, while a mild transition (MT) is a stepwise transition by 1 °C d⁻¹. We find a greater decrease in methane production with ST (88.8%) compared to MT (38.9%) during the transition period. ST mode overproduced reactive oxygen species by 1.6-fold, increased membrane permeability by 2.2-fold, and downregulated microbial energy metabolism by 25.1%, leading to increased apoptosis of anaerobes by 1.9-fold and release of intracellular substances by 2.9-fold, further constraining methanogenesis. The higher (1.6 vs. 1.1 copies per <em>gyr</em>A) metabolic activity of acetate-dependent methanogenesis implied more efficient methane production in a steady mesophilic, MT-mediated system. Metagenomic binning and network analyses indicated that ST induced dysbiosis in keystone species and greatly enhanced microbial functional redundancy, causing loss of microbial syntrophic interactions and redundant metabolic pathways. In contrast, the greater microbial interconnections (average degrees 44.9 vs. 22.1) in MT at a steady mesophilic state suggested that MT could better maintain necessary system functionality and stability through microbial syntrophy or specialized pathways. Adopting MT to transform thermophilic digesters into mesophilic digesters is feasible and could potentially enhance the further optimization and broader application of practical anaerobic engineering.</p></div>","PeriodicalId":34434,"journal":{"name":"Environmental Science and Ecotechnology","volume":"21 ","pages":"Article 100440"},"PeriodicalIF":12.6,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666498424000541/pdfft?md5=1e3a5fe99d8cb02cc135ef6228bedee4&pid=1-s2.0-S2666498424000541-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141391364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}