Frontiers in Nanotechnology最新文献

{"title":"Detection and quantification of antiviral drug tenofovir using silver nanoparticles and surface enhanced Raman spectroscopy (SERS) with spatially resolved hotspot selection","authors":"Marguerite R. Butler, Jana Hrncirova, Terry A. Jacot, Sucharita Dutta, Meredith R. Clark, Gustavo F. Doncel, John B. Cooper","doi":"10.3389/fnano.2023.1270474","DOIUrl":"https://doi.org/10.3389/fnano.2023.1270474","url":null,"abstract":"This study introduces a convenient and ultra-sensitive method of detection and quantification of the antiviral drug, tenofovir (TFV), by surface-enhanced Raman spectroscopy (SERS). Novel spatially resolved instrumentation for spectral acquisition and subsequent statistical analysis for hot spot selection was developed for convenient quantification of TFV in an aqueous matrix. Methods of statistical analysis include the use of partial least squares (PLS) regression vector analysis and spectral ranking by quality indices computed using CHAOS theory. Hydroxylamine-reduced Ag colloidal nanoparticles evaporated to dryness on an aluminum well-plate were used as the SERS substrate. To our knowledge, quantification of TFV down to 25 ng/mL by SERS, comprising clinically relevant concentrations, has not been previously reported. Furthermore, in this work we propose a novel method of quantification of aqueous TFV standards by SERS using statistical treatment of data by PLS and CHAOS theory. Based on these data, we propose future studies to develop a method of TFV detection and quantification in biological samples, beneficial to clinicians for rapid assessment of drug adherence during the treatment and prevention of viral diseases.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135884343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles","authors":"Alexandra Paxinou, Elena Marcello, Vittoria Vecchiato, Lara Erman, Edward Wright, Brendon Noble, Adele McCormick, Pooja Basnett","doi":"10.3389/fnano.2023.1243056","DOIUrl":"https://doi.org/10.3389/fnano.2023.1243056","url":null,"abstract":"Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44892847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining metal nanoparticles and nanobodies to boost the biomedical imaging in neurodegenerative diseases","authors":"A. Sanna, A. Quarta, N. Pieroni, B. Parodi, F. Palermo, I. Bukreeva, M. Fratini, L. Massimi, D. Simeone, X. Le Guével, A. Bravin, E. Quintiero, I. Viola, G. Gigli, N. Kerlero de Rosbo, L. Sancey, A. Cedola","doi":"10.3389/fnano.2023.1236810","DOIUrl":"https://doi.org/10.3389/fnano.2023.1236810","url":null,"abstract":"Introduction: In the study of neurodegenerative diseases, the possibility to follow the fate of specific cells or molecules within the whole body would be a milestone to better understand the complex evolution of disease mechanisms and to monitor the effects of therapies. The techniques available today do not allow the visualization of disease-relevant cells within the whole tridimensional biological context at high spatial resolution.Methods: Here we show the results from the first validation steps of a novel approach: by combining the conjugate nanobodies anti-glial fibrillary acidic protein (GFAP) and metal-nanoparticles (i.e. 2 nm gold NP) with X-ray phase contrast tomography (XPCT) we would be able to obtain a tridimensional visualization and identification of cells of interest together with the surrounding tissue and the vascular and neuronal networks.Results: By exploiting the X-ray attenuation properties of metal nanoparticles and the specific targeting capabilities of nanobodies, we could give XPCT the specificity it presently lacks, making it no longer a pure morphological but a molecular and targeted imaging technique. In our case, we synthesized and characterized Gold-NP/GFAP nanobody to target the astrocytes of mouse brain.Discussion: The results of the first tests presented in this paper have provided us with information on the feasibility of the approach, encouraging us to carry out further experiments in order to achieve the ultimate goal of setting up this new imaging technique.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47689320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A short appraisal of nanodiamonds in drug delivery and targeting: recent advancements","authors":"Dilpreet Singh, Supriya Ray","doi":"10.3389/fnano.2023.1259648","DOIUrl":"https://doi.org/10.3389/fnano.2023.1259648","url":null,"abstract":"Nanodiamonds have emerged as a powerful tool in the field of drug delivery, offering exciting recent advancements and promising future prospects. Recent research has focused on enhancing drug loading efficiency, targeted delivery, and precision medicine using nanodiamonds. Strategies such as surface functionalization, polymer coatings, and encapsulation techniques have been optimized to improve drug loading, stability, and controlled release. Nanodiamonds have shown potential in targeted drug delivery through the incorporation of targeting ligands, enabling site-specific drug delivery and improved therapeutic outcomes. Additionally, nanodiamonds allow for combination therapy by carrying multiple drugs simultaneously, opening avenues for synergistic treatments. Stimuli-responsive nanodiamonds have been developed for precise and controlled drug release. Overcoming biological barriers, such as the blood-brain barrier, has been investigated by modifying nanodiamonds’ surfaces. Recent studies have highlighted innovative strategies for loading a variety of therapeutic cargoes onto nanodiamond platforms, including small molecules, proteins, nucleic acids, and even gene-editing tools. Future prospects include the integration of nanodiamonds with emerging technologies and addressing biocompatibility and safety concerns. Despite the need for further research and clinical studies, the recent advancements and future prospects of nanodiamonds in drug delivery suggest their potential to revolutionize the field and enhance patient care.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46286525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identity crisis of nanostructures inside the human body: a perspective on inflammation","authors":"Sarmistha Saha, L. Saso","doi":"10.3389/fnano.2023.1256952","DOIUrl":"https://doi.org/10.3389/fnano.2023.1256952","url":null,"abstract":"Advances in nanotechnology have attracted a lot of potential medical applications, such as therapeutic agents, diagnostics, and theranostics for complex diseases. The intersection of nanotechnologies, molecular and cell biology, and medicine can function to improve human health and quality of life, making healthcare a primary target of nanotechnology research. However, this seems like a promising future, ethical, health, and safety concerns must be considered before a reasoned evaluation of the situation can be made. Most nanostructures, however, typically fail to reach their intended targets because they get trapped inside innate immune cells. Since little is known about how nanomaterials and nanotechnologies change their identity inside the biological system, there is a wide-ranging discussion on possible concerns. In this regard, we present a perspective on how biological systems may interact with nanoscale materials and how that interaction might affect cellular recognition of nanostructures. We will also discuss dynamic modifications of the nanomaterials inside biological systems and, in particular, inflammation responses.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41333032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: New generation nano-biomaterials and their potential application in drug delivery and bio-sensing","authors":"Bijayananda Panigrahi, Suresh K. Verma, Sourav Das, Abhishek Kumar","doi":"10.3389/fnano.2023.1260792","DOIUrl":"https://doi.org/10.3389/fnano.2023.1260792","url":null,"abstract":"","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44251997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis and characterization of silver nanoparticles generated from peels of Solanum tuberosum (potato) and their antibacterial and wastewater treatment potential","authors":"None Deepa, Raunak Dhanker, Ram Kumar, Shashank S. Kamble, None Kamakshi, Shubham Goyal","doi":"10.3389/fnano.2023.1213160","DOIUrl":"https://doi.org/10.3389/fnano.2023.1213160","url":null,"abstract":"Global food production consumes a large fraction of energy budget, land area, and freshwater; however, a larger fraction of the produce is lost or unutilized, which has potential to produce useful products for human use. The biogenic synthesis of silver nanoparticles from such waste food appears to be a promising strategy. A conservative estimate of 70–140 thousand tons of potato peels is produced annually by food-chain companies globally; however, they are primarily utilized to produce substandard feed for livestock or manure. For the formation of highly profitable compounds, enhancement of value, and the process of extraction, such as nanocomposite, organic antioxidants, and organic meal inclusions, potato peels can be used as a cheap, productive, and readily available source of raw material. In the present research, silver nanoparticles (AgNPs) were extracted from the peels of potato ( Solanum tuberosum ) . The fabrication of potato peel-derived AgNPs was established using UV-visible spectroscopy analysis. Approaches like X-ray diffraction (XRD), attenuated total reflection-infrared (ATR-IR) spectroscopy analysis, and field emission scanning electron microscopy (FESEM) were used to determine the characteristics of the AgNPs. Additionally, strains of Gram-positive bacteria such as Staphylococcus aureus ( S. aureus ) (ATCC 25923) and Gram-negative bacteria such as Escherichia coli ( E. coli ) (ATCC 25922) were used to determine the antibacterial activity of AgNPs via the disc diffusion technique. The antibacterial properties of AgNPs could help protect food from microbial contamination. Furthermore, AgNPs were tested for their potential application in purification of industrial wastewater. The results revealed that AgNPs derived from the potato peels could be used in industrial and biomedical applications and possess excellent antibacterial activity. Our research suggests that AgNPs can be extracted from a safe and ecofriendly fabrication technique from largely unused potato peels that have a great potential for inhibiting the bacterial growth and for the in situ purification of wastewater in the upcoming years. Therefore, besides value addition to the farm produce, such recycling of potato peels is likely to reduce the burden of the solid waste volumes in agro-centers, kitchen wastes, and food industries across the globe.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135114523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable nano-interventions to enhance crop yield, anthocyanin content, and marketability of onion (Allium cepa)","authors":"Himanshi Jangir, Brijesh Kaler, Gaurav Srivastava, M. Das","doi":"10.3389/fnano.2023.1256439","DOIUrl":"https://doi.org/10.3389/fnano.2023.1256439","url":null,"abstract":"Introduction: Agriculture predominantly follows three farming systems: natural, organic (organic manures for soil nutrition), and agrochemical intensive. Agrochemical-intensive farming uses synthetic fertilizers, insecticides, pesticides, and weedicides. It holds the most prominent place in the modern agriculture business. Humankind realizes that over-reliance on synthetic agrochemicals has resulted in the declining health of the ecosystem’s soil, water, and living species. It must explore strategies to reduce synthetic agrochemicals without compromising agricultural production to restore the ecological balance. Nano-formulations help lower dosages of agrochemicals, leading to the emergence of nano-agriculture. However, the critical challenge is how we could exploit nanomaterials’ power to selectively improve crop plants’ metabolic performance so that it has a better competitive edge in the field to withstand the nutrient-seeking pressure exerted by other plants.Methods: Here we have achieved the same by root treatment of the onion crop and then growing them in the presence of organic goat manure.Results: In a 2 years field trial with onion crops, we report an increase in yield through root treatment with nano-pyrite (FeS2) plus soil application of goat dropping (Test) as compared to the use of goat dropping alone (Control). In the first-year field trial (November 2018- April 2019), the total biomass (bulb + leaf) weight of the test sample was 4.75 kg (n = 86), while control samples weighed 3.5 kg (n = 83). The total bulb weight for the control and test was 2.6 and 3.6 kg, respectively. In the second-year field trial (December 2019- April 2020), the total biomass of the test sample was 2.65 kg (n = 64), while control samples weighed 2.30 kg (n = 64). We observed a yield-boosting effect of root treatment with nano-pyrite plus soil application of goat dropping in onion crops. As monitored by spectroscopic absorbance, there is a significant increase in the anthocyanin content in test bulbs (0.069) compared to control bulbs (0.02). Similarly, the relative flavanol content in test leaves (0.253) was significantly higher compared to the control samples (0.086). We have integrated nano-agriculture and organic farming, resulting in a hybrid form; nano-organic farming bolsters the metabolic fitness of the onion (Allium cepa) to achieve sustainable food production.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42610596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probing magnetization dynamics of iron oxide nanoparticles using a point-probe magneto-optical method","authors":"Xuyiling Wang, D. Cabrera, Ying Yang, N. Telling","doi":"10.3389/fnano.2023.1214313","DOIUrl":"https://doi.org/10.3389/fnano.2023.1214313","url":null,"abstract":"Magnetic nanoparticles (MNPs) are promising as local heat generators for magnetic hyperthermia under AC magnetic fields. The heating efficacy of MNPs is determined by the AC hysteresis loop area, which in turn is affected by the dynamic magnetic properties of the nanoparticles. Whilst inductive-based AC magnetometers can measure the average magnetic behavior of samples, the use of the magneto-optical Faraday effect with a focused laser spot allows point-probe measurements to be made, and without some of the magnetic field limitations imposed by inductive methods. In this work, the AC magnetic properties of different sized iron oxide MNPs in suspension were measured by AC magnetometry and AC susceptibility techniques. AC hysteresis loops measured by magneto-optical magnetometry were validated using a commercial inductive AC magnetometer, and compared to the magnetization relaxation behavior revealed by fitting the AC susceptibility data. The spatial sensitivity of the point-probe magneto-optical method is also demonstrated by measuring the AC hysteresis loop from large (>1 μm) MNP aggregates dried onto glass slides. These aggregated particles are found to be magnetically softer than in their suspension form, suggesting interparticle coupling mechanisms could occur when the nanoparticles form dense aggregates.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48766840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the bacterial synthesis of metal nanoparticles","authors":"A. Campaña, Athanasios Saragliadis, P. Mikheenko, D. Linke","doi":"10.3389/fnano.2023.1216921","DOIUrl":"https://doi.org/10.3389/fnano.2023.1216921","url":null,"abstract":"Metal nanoparticles have attracted considerable attention due to their astounding potential for a wide range of commercial applications. From targeted drug delivery and antimicrobial agents to electronics, metal nanoparticles seem to have immeasurable prospects in all areas of science. However, modern industrial production frequently involves complex procedures, large amounts of energy, utilizes strong chemical solvents, or produces hazardous waste. Biological synthesis has been proposed as an alternative for simpler, inexpensive, and more eco-friendly metal nanoparticle production. Microorganisms possess multiple mechanisms to transport, regulate and bind metal ions that may result in the biosynthesis of nanoparticles. They can synthesize even complex bimetallic nanoparticles, which are difficult to produce with normal chemical and physical processes. A better understanding of bacteria-metal interactions might thus pave the way for a wide array of industrial applications. This review will summarize the current methods for metal nanoparticle synthesis, with a focus on the microbial (bio) synthesis of nanoparticles. We will describe the general mechanisms of bacteria-metal ion interactions, including cellular uptake and the subsequent reduction into nanoparticles. Protocols for the production of metal-based nanoparticles of relevant elements with different bacterial strains are compiled and the current challenges in bacterial synthesis of metal nanoparticles in the industry are discussed.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49162764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}