Biomedical Research Journal最新文献_第4页

Telomere length maintenance mechanisms in cancer 癌症端粒长度维持机制

Biomedical Research Journal Pub Date : 2018-04-01 DOI: 10.4103/2349-3666.240298

Ekta Khattar

引用次数: 0

Evaluation of housekeeping genes for studies in breast cancer cell lines treated with progesterone 孕酮处理乳腺癌细胞系研究中管家基因的评价

Biomedical Research Journal Pub Date : 2018-04-01 DOI: 10.4103/2349-3666.240301

A. Parab, Jaykumar V Kambli, Sujata Hake, N. Joshi

{"title":"Evaluation of housekeeping genes for studies in breast cancer cell lines treated with progesterone","authors":"A. Parab, Jaykumar V Kambli, Sujata Hake, N. Joshi","doi":"10.4103/2349-3666.240301","DOIUrl":"https://doi.org/10.4103/2349-3666.240301","url":null,"abstract":"Real-time semi-quantitative PCR (qPCR) is extensively used to assess variations in gene expressions resulting from pathological conditions or biological responses. Normalization of qPCR data is required to control innate variations that occur during experimental procedures. Endogenous control genes (ECs), used for normalization of the data should be expressed constitutively and consistently across treatments groups. The aim of the present study was to identify the most suitable endogenous control genes for qPCR based analyses to study responses in breast cancer cell lines following exposure to Progesterone (P4). The expression and validity of five candidate ECs (PUM1, RPS13, RPL13A, TBCA and PSMB) were determined in three breast cancer cell lines following exposure to Progesterone. Gene expression data was analyzed using three methods, Normfinder, Bestkeeper, and Comparative delta Ct method. A significant difference in variance of expression levels of individual ECs under different growth conditions was observed. Expression of PUM1 and PSMB was minimally affected in breast cancer cell lines under the experimental conditions. Indeed, in the progesterone treated cells,PUM1 and PSMB were identified as the most stable EC genes by all three methods while TBCAand RPS13 were least stable. In breast cancer cell lines, our results highlight PSMB as another stably expressed endogenous control gene besides the previously identified PUM1. PSMB gene expression may thus provide an additional parameter for studies in breast cancers.","PeriodicalId":34293,"journal":{"name":"Biomedical Research Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83859155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Estimation of epidemiological parameters for historicalm ship outbreaks of influenza 历史上船舶流感暴发流行病学参数的估计

Biomedical Research Journal Pub Date : 2018-04-01 DOI: 10.4103/2349-3666.240299

Praveen Kumar, Anjali Kshirsagar, P. Shil

引用次数: 1

Stability-indicating HPLC method for primaquine diphosphate: An application to niosomal formulation 稳定性指示高效液相色谱法测定二磷酸伯氨喹在乳质体制剂中的应用

Biomedical Research Journal Pub Date : 2018-04-01 DOI: 10.4103/2349-3666.240300

M. Thakkar, S. Brijesh

{"title":"Stability-indicating HPLC method for primaquine diphosphate: An application to niosomal formulation","authors":"M. Thakkar, S. Brijesh","doi":"10.4103/2349-3666.240300","DOIUrl":"https://doi.org/10.4103/2349-3666.240300","url":null,"abstract":"A novel, rapid, and precise stability-indicating gradient reverse-phase high performance liquid chromatographic method for the quantitative determination of primaquine diphosphate and its impurities was developed and validated. Efficient chromatographic separation was achieved on an Inertsil ODS-3V column (150 mm × 4.6 mm, 5.0 μm) with mobile phase containing phosphate buffer (0.05 M KH2PO4, pH 4.5) in gradient combination with acetonitrile at a flow rate of 1 mL/min and the analyte was monitored using a photo-diode array detector at a wavelength of 254 nm. The forced-degradation of primaquine diphosphate was carried out by exposing it to acidic, basic, neutral, thermal, photolytic, and oxidative stress conditions. The peaks of the degradation products obtained were well resolved from that of primaquine diphosphate, indicating that the method developed was specific and stability-indicating. Further, the method was validated according to the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines, with respect to parameters such as specificity, precision, linearity, accuracy and robustness. The developed method could also be used for the quantification of primaquine diphosphate encapsulated in niosome formulation.","PeriodicalId":34293,"journal":{"name":"Biomedical Research Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88063283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Niosomes as Transdermal Drug Delivery System Niosomes作为经皮给药系统

Biomedical Research Journal Pub Date : 2018-01-10 DOI: 10.4103/2349-3666.244767

Amruta N Parmar, S. Brijesh

{"title":"Niosomes as Transdermal Drug Delivery System","authors":"Amruta N Parmar, S. Brijesh","doi":"10.4103/2349-3666.244767","DOIUrl":"https://doi.org/10.4103/2349-3666.244767","url":null,"abstract":"attempting to improve the efficiency of utilization of drugs for treatment of various diseases. In this endeavour, drug delivery systems have helped greatly by achieving reduced dose, dosage frequency, and side effects; better patient compliance; and maximum concentration of the drug at the target site. Recent years have seen an unprecedented growth in the use of nanotechnology in designing drug delivery systems. Use of nanostructured drug delivery systems has changed the landscape of pharmaceutical and biotechnology industries. Nanocarriers offer advantages such as, (1) encapsulation and prevention of the drug from degradation, (2) improved delivery of poorly water soluble drugs, (3) targeted drug delivery, (4) co-delivery of multiples drugs with varying solubility or modes of action, (5) controlled release, and (6) production on a large scale (Farokhzad and Langer, 2009). Drug delivery systems have been synthesized using substances varying from molecules of biological origin to inorganic or chemically synthesized substances. Biological molecules that have been used include, gelatin, albumin and chemical substances include various polymers and solid metal-containing NPs. Organic nanoplatforms include liposomes, polymeric nanoparticles, polymer-drug conjugates, polymeric micelles, hydrogel nanoparticles, proteinbased nanoparticles, and dendrimers; whereas inorganic platforms include noble metal nanoparticles, superparamagnetic nanoparticles, ceramic nanoparticles, carbon-based nanomaterials, and integrated nanocomposite particles (Bamrungsap et al., 2012).","PeriodicalId":34293,"journal":{"name":"Biomedical Research Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87795539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Odontogenic Fibroma of Maxilla: A Rare Clinical Entity 上颌骨牙源性纤维瘤:一种罕见的临床实体

Biomedical Research Journal Pub Date : 2018-01-10 DOI: 10.4103/2349-3666.244772

S. Pol, A. Dass, N. Gupta

引用次数: 0

Ce 3+ Sensitized YPO 4 :Tb 3+ as Luminescent Probe for Selective Detection of Cu 2+ Ions ce3 +敏化的YPO 4: tb3 +作为发光探针选择性检测cu2 +离子

Biomedical Research Journal Pub Date : 2018-01-10 DOI: 10.4103/2349-3666.244771

N. Singh, Ranjoy Wangkhem, M. Ahmed

引用次数: 0

Surfactant Modified Graphene Oxide for the Detection of Dopamine 表面活性剂修饰氧化石墨烯对多巴胺的检测

Biomedical Research Journal Pub Date : 2018-01-10 DOI: 10.4103/2349-3666.244768

Diptesh G. Naik, B. R. Naik, Akshay V. Salkar, Vrushali S. Joshi

引用次数: 0

Drug resistance in leukemia: Remediation by natural means 白血病的耐药性:自然手段的补救

Biomedical Research Journal Pub Date : 2017-04-01 DOI: 10.4103/2349-3666.240593

M. Roy, A. Mukherjee, S. Mukherjee, J. Biswas

{"title":"Drug resistance in leukemia: Remediation by natural means","authors":"M. Roy, A. Mukherjee, S. Mukherjee, J. Biswas","doi":"10.4103/2349-3666.240593","DOIUrl":"https://doi.org/10.4103/2349-3666.240593","url":null,"abstract":"Leukemia is a common cancer in the paediatric group with good prognosis and the overall survival rate for leukemia has improved over the years. However, despite the chemopreventive agents and better diagnostic and therapeutic strategies, novel strategies and approaches shows better prognosis and overall survival, particularly if detected early. The common modality of haematological malignancy management is chemotherapy. Although good response is noted early on treatment, resistance to the chemotherapy is observed later due to the drug resistance, may be due to increased expression of P-glycoprotein (Pgp) and multidrug resistance protein (MRP1), which may be reversed by inhibitors of the proteins. However, adverse side effects of the chemotherapeutic agents are a cause of concern in several patients. Hence, plant derived molecules (phytochemicals) may be considered as an alternative to the synthetic inhibitors. Phytochemicals possess not only chemopreventive and chemotherapeutic potential, but also can be used for prevention and reversal of drug resistance. Phytochemicals are generally nontoxic, economic and minimal adverse effects are observed. Potential phytochemicals may be used as stand-alone or in combination for cancer treatment.","PeriodicalId":34293,"journal":{"name":"Biomedical Research Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78354799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical PET, SPECT, CT, MRI and optical imaging in cancer research: An overview 临床前PET、SPECT、CT、MRI和光学成像在癌症研究中的应用综述

Biomedical Research Journal Pub Date : 2017-01-01 DOI: 10.4103/2349-3666.240599

P. Chaudhari

{"title":"Preclinical PET, SPECT, CT, MRI and optical imaging in cancer research: An overview","authors":"P. Chaudhari","doi":"10.4103/2349-3666.240599","DOIUrl":"https://doi.org/10.4103/2349-3666.240599","url":null,"abstract":"Cancer research is primarily dependent on rodent models. There has been a huge advancement in anticancer drug discovery in the last two decades, which is mainly due to improved animal models and introduction of non-invasive imaging technologies. Non-invasive imaging technology plays an important role in cancer and other biomedical research areas including neurology, cardiology, nanotechnology and stem cell biology. The intricate pathways in cancer initiation, progression and metastasis can be visualized and the processes can be analyzed quantitatively using these techniques. Robust preclinical data is the backbone of anticancer drug discovery, which can be obtained precisely using imaging technology. This review is focused on the dedicated preclinical imaging modalities such as Positron Emission Tomography (PET), Single Photon Emission Computed Tomography (SPECT), X-ray Computed Tomography (CT), Magnetic Resonance Imaging (MRI), Optical imaging and Cerenkov imaging. This paper discusses their present roles in basic and translational research towards evaluation of suitability of target molecules for further clinical trials.The unique features of the imaging modalities enable them to provide information necessary for understanding structural, functional and molecular processes involved in various stages of cancer development thus bridging the gap between bench and bedside.","PeriodicalId":34293,"journal":{"name":"Biomedical Research Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91356539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0