发布求助
文献互助 智能选刊 最新文献

STAR Protocols最新文献

筛选
英文 中文
Protocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system.
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-12-10 DOI: 10.1016/j.xpro.2024.103519
Ying Lin, Shekhar Singh, Chong Xu, Zeyu Wang, Cailin Feng, Dongyang Jiang, Lingfeng Luo, Weiming Li, Wenliang Che, Guofu Zhu
{"title":"Protocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system.","authors":"Ying Lin, Shekhar Singh, Chong Xu, Zeyu Wang, Cailin Feng, Dongyang Jiang, Lingfeng Luo, Weiming Li, Wenliang Che, Guofu Zhu","doi":"10.1016/j.xpro.2024.103519","DOIUrl":"10.1016/j.xpro.2024.103519","url":null,"abstract":"<p><p>Notch signaling is a pivotal regulator in the vascular system that is essential for development, angiogenesis, and maintaining vascular homeostasis. Here, we present a protocol for tyramide signal amplification (TSA) immunohistochemistry, tailored explicitly for detecting Notch signaling components in vascular tissues. We describe steps for utilizing tailored antigen retrieval techniques, specific blocking solutions, and a complex of avidin/biotin-horseradish peroxidase conjugate with tyramide, along with optimized washing steps. For complete details on the use and execution of this protocol, please refer to Zhu et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103519"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining.
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-12-11 DOI: 10.1016/j.xpro.2024.103482
Beatriz Maio, Nagore Elu, Cristina Martinez-Gonzalez, Emily K Osterweil, Susana R Louros
{"title":"Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining.","authors":"Beatriz Maio, Nagore Elu, Cristina Martinez-Gonzalez, Emily K Osterweil, Susana R Louros","doi":"10.1016/j.xpro.2024.103482","DOIUrl":"10.1016/j.xpro.2024.103482","url":null,"abstract":"<p><p>cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis. For complete details on the use and execution of this protocol, please refer to Louros et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103482"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol to evaluate virus-specific CD4+ and CD8+ T cell responses by spectral flow cytometry. 通过光谱流式细胞仪评估病毒特异性 CD4+ 和 CD8+ T 细胞反应的方案。
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-12-12 DOI: 10.1016/j.xpro.2024.103497
Xin-Jing Zhao, Sheng Zhang, Wang-Qian Wei, Qiang Xu, Chen-Long Lv, Guo-Lin Wang, Li-Qun Fang
{"title":"Protocol to evaluate virus-specific CD4<sup>+</sup> and CD8<sup>+</sup> T cell responses by spectral flow cytometry.","authors":"Xin-Jing Zhao, Sheng Zhang, Wang-Qian Wei, Qiang Xu, Chen-Long Lv, Guo-Lin Wang, Li-Qun Fang","doi":"10.1016/j.xpro.2024.103497","DOIUrl":"10.1016/j.xpro.2024.103497","url":null,"abstract":"<p><p>T cell responses play an important role in viral clearance and prevention of infection. Here, we present a protocol to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell responses using spectral flow cytometry. We describe steps for peripheral blood mononuclear cell (PBMC) thawing and stimulation with SARS-CoV-2 spike peptide pools. We then describe detailed procedures for cell confining, staining, and fixation. This protocol has potential application in spectral flow cytometry on other virus-specific T cell responses. For complete details on the use and execution of this protocol, please refer to Zhao et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103497"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol for generating brain metastatic tumor cells through repeated intracardiac injections in mice.
IF 1.3
STAR Protocols Pub Date : 2024-12-20 DOI: 10.1016/j.xpro.2024.103531
Li Wang, Xiaoxia Chen, Chengtao Dong, Shengqi Yin, Li Liang, Aidong Zhou
{"title":"Protocol for generating brain metastatic tumor cells through repeated intracardiac injections in mice.","authors":"Li Wang, Xiaoxia Chen, Chengtao Dong, Shengqi Yin, Li Liang, Aidong Zhou","doi":"10.1016/j.xpro.2024.103531","DOIUrl":"https://doi.org/10.1016/j.xpro.2024.103531","url":null,"abstract":"<p><p>Here, we present a protocol for generating the lung cancer cell line, LLC1-BMT5, with highly brain metastatic tropism through multiple rounds of in vivo selection. We describe steps for establishing the brain metastases (BrMs) mouse model through intracardiac injection of cancer cells. We then detail procedures for obtaining brain metastatic subpopulations, in vivo selection of LLC1-BMT5 cells, and validating metastatic potential. This protocol will facilitate the study of the molecular mechanisms of BrMs and the development of anti-BrM treatment strategies. For complete details on the use and execution of this protocol, please refer to Wang et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 1","pages":"103531"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol for the assessment, improvement, and harmonization of somatic variant calling using ONCOLINER.
IF 1.3
STAR Protocols Pub Date : 2024-12-20 DOI: 10.1016/j.xpro.2024.103533
Rodrigo Martín, Nicolás Gaitán, David Torrents
{"title":"Protocol for the assessment, improvement, and harmonization of somatic variant calling using ONCOLINER.","authors":"Rodrigo Martín, Nicolás Gaitán, David Torrents","doi":"10.1016/j.xpro.2024.103533","DOIUrl":"https://doi.org/10.1016/j.xpro.2024.103533","url":null,"abstract":"<p><p>The interoperability of variant identification pipelines is fundamental for achieving consistent clinical care across oncology research centers and hospitals. Here, we present a protocol for using ONCOLINER, a platform for the assessment, improvement, and harmonization of somatic variant discovery of multiple pipelines. We describe steps for acquiring benchmarking datasets and executing the user variant calling pipeline. We then detail the procedures for performing analyses to produce user-friendly reports showing the quality, scope, and applicable improvements for each tumor genome analysis. For complete details on the use and execution of this protocol, please refer to Martín et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 1","pages":"103533"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol to study ex vivo T cell priming by conventional dendritic cells from the mouse spleen.
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-12-11 DOI: 10.1016/j.xpro.2024.103382
Mitra Ashayeripanah, Jose A Villadangos
{"title":"Protocol to study ex vivo T cell priming by conventional dendritic cells from the mouse spleen.","authors":"Mitra Ashayeripanah, Jose A Villadangos","doi":"10.1016/j.xpro.2024.103382","DOIUrl":"10.1016/j.xpro.2024.103382","url":null,"abstract":"<p><p>Conventional dendritic cells (cDC) are professional antigen-presenting cells able to prime naive T cells. Here, we present a protocol for ex vivo T cell priming by murine splenic cDC. We describe the steps of injecting fluorescently labeled antigens to mice, purifying antigen-bearing cDC, and priming antigen-specific T cells ex vivo. This protocol is suitable for studying the T cell priming function of cDC in various murine models and helps factor in the effect of the microenvironment on cDC ability to uptake and process antigens. For complete details on the use and execution of this protocol, please refer to Ashayeripanah et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103382"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol for monitoring phagocytosis of cancer cells by TAM-like macrophages using imaging cytometry. 利用成像细胞仪监测 TAM 样巨噬细胞吞噬癌细胞的过程。
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-09-18 DOI: 10.1016/j.xpro.2024.103320
Alok K Mishra, Shahid Banday, Ritesh P Thakare, Sunil K Malonia, Michael R Green
{"title":"Protocol for monitoring phagocytosis of cancer cells by TAM-like macrophages using imaging cytometry.","authors":"Alok K Mishra, Shahid Banday, Ritesh P Thakare, Sunil K Malonia, Michael R Green","doi":"10.1016/j.xpro.2024.103320","DOIUrl":"10.1016/j.xpro.2024.103320","url":null,"abstract":"<p><p>Here, we present a protocol for monitoring phagocytosis by M2-type macrophages using automated counting of phagocytic events with an imaging cytometer. We describe steps for isolating and differentiating peripheral blood mononuclear cell (PBMC)-derived monocytes into M2-like macrophages, preparing cancer cells expressing a green fluorescence marker, labeling with a pH-sensitive dye, and co-culturing with macrophages. We then outline procedures for enumerating phagocytic events using an imaging cytometer. For complete details on the use and execution of this protocol, please refer to Mishra et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103320"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11426124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142297136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer. 利用生物发光共振能量转移测量和分析哺乳动物细胞中蛋白质相互作用的方案。
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-09-28 DOI: 10.1016/j.xpro.2024.103348
Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa
{"title":"Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer.","authors":"Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa","doi":"10.1016/j.xpro.2024.103348","DOIUrl":"10.1016/j.xpro.2024.103348","url":null,"abstract":"<p><p>Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of HEK293-EBNA cells. We describe steps for seeding, transfecting, and replating cells. We then detail procedures for their preparation for BRET measurements on a CLARIOstar microplate reader and detailed data analysis. For complete details on the use and execution of this protocol, please refer to Steffen et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103348"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol to study cis-regulatory activity of GWAS loci for specific gene promoters in human primary astrocytes using luciferase reporter assay. 利用荧光素酶报告分析法研究 GWAS 基因位点对人类原代星形胶质细胞中特定基因启动子的顺式调控活性。
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-09-25 DOI: 10.1016/j.xpro.2024.103338
Sheridan H Littleton, Struan F A Grant
{"title":"Protocol to study cis-regulatory activity of GWAS loci for specific gene promoters in human primary astrocytes using luciferase reporter assay.","authors":"Sheridan H Littleton, Struan F A Grant","doi":"10.1016/j.xpro.2024.103338","DOIUrl":"10.1016/j.xpro.2024.103338","url":null,"abstract":"<p><p>Genome-wide association study loci likely influence disease risk through cis-regulation of nearby genes. We present a protocol for measuring the cis-regulatory activity of risk variants on specific gene promoters in human primary astrocytes using luciferase reporter assays. We describe steps for transfection, conducting the assay, and data analysis. The transfection could be adapted to enable validation of putative risk variants in any cell type. For complete details on the use and execution of this protocol, please refer to Littleton et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103338"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol for isolating specific C. elegans neuron types for bulk and single-cell RNA sequencing. 用于大量和单细胞 RNA 测序的特定秀丽隐杆线虫神经元类型分离规程。
IF 1.3
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-11-06 DOI: 10.1016/j.xpro.2024.103439
Seth R Taylor, Rebecca D McWhirter, Brittany K Matlock, David K Flaherty, David M Miller
{"title":"Protocol for isolating specific C. elegans neuron types for bulk and single-cell RNA sequencing.","authors":"Seth R Taylor, Rebecca D McWhirter, Brittany K Matlock, David K Flaherty, David M Miller","doi":"10.1016/j.xpro.2024.103439","DOIUrl":"10.1016/j.xpro.2024.103439","url":null,"abstract":"<p><p>The C. elegans nervous system is compact and well described, ideal for identifying genetic programs that drive neuron-specific development, function, and connectivity. Here, we present a protocol for isolating specific neuron types for gene expression profiling by bulk or single-cell RNA sequencing. We describe steps for worm synchronization, dissociation, and fluorescence-activated cell sorting (FACS) isolation. We then detail procedures for RNA extraction and preparing cells for single-cell sequencing. This protocol is applicable to the isolation of individual cell types from larval and adult animals. For additional details on the use and execution of this protocol, see Taylor et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103439"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信