培养细胞中cgamp诱导的STING寡聚化检测方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-06-20 Epub Date: 2025-03-21 DOI:10.1016/j.xpro.2025.103709

Lizhi Liu, James L Manley

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引用次数: 0

摘要

STING（干扰素基因刺激因子）在先天免疫反应中起关键作用，包括病毒感染、癌症免疫和一些自身免疫性疾病。在这里，我们提出了一种检测STING寡聚化的协议，这是STING激活的关键步骤。我们描述了在HEK293T细胞中表达STING的步骤，通过2‘3’环GMP-AMP （cGAMP）处理激活STING，并通过非还原SDS-PAGE和蓝色原生PAGE分析STING寡聚化。该方案是有用的研究STING功能，调节和失调。有关使用和执行本协议的完整细节，请参阅Liu和manley。

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Protocol for detection of cGAMP-induced STING oligomerization in cultured cells.

STING (stimulator of interferon genes) plays a critical role in the innate immune response, including in viral infection, cancer immunity, and several autoimmune diseases. Here, we present a protocol for detection of STING oligomerization, a critical step in STING activation. We describe steps for expressing STING in HEK293T cells, activating STING by 2'3' cyclic GMP-AMP (cGAMP) treatment, and analyzing STING oligomerization by non-reducing SDS-PAGE and blue native PAGE. This protocol is useful for studies of STING functions, regulation, and dysregulation. For complete details on the use and execution of this protocol, please refer to Liu and Manley.¹.

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